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研究生:涂哲彰
研究生(外文):Che-Chang Tu
論文名稱:表面增強型拉曼結合銀奈米顆粒應用於乳酸分子定量量測
論文名稱(外文):Surface Enhanced Raman Scattering Spectroscopy for Quantitative Lactate Measuring using Self-assembly Silver Nanoparticles
指導教授:江惠華江惠華引用關係
指導教授(外文):Huihua Kenney Chiang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生醫光電工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:英文
論文頁數:55
中文關鍵詞:表面增強拉曼光譜儀乳酸定量乙醇銀奈米顆粒
外文關鍵詞:SERSLACTATEALCOHOLSURFACE ENHANCED RAMAN SCATTERINGSILVER NANOPARTICLESQuantitative
相關次數:
  • 被引用被引用:2
  • 點閱點閱:228
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  • 下載下載:37
  • 收藏至我的研究室書目清單書目收藏:0
摘要
乳酸為無氧糖解中的副產物,已經在某些臨床情況下用來預測疾病的嚴重度。過度乳酸的堆積將會造成生理環境中的酸鹼失衡。在本論文中,我們將應用表面增強拉曼散射技術配合高解析度的拉曼光譜儀,來對生理濃度的乳酸分子做量測。我們採用更改後、有別於傳統的膠體銀製備方法,以避免在進行表面增強拉曼散射時,因之前的氧化還原反應過程產物,所產生不正常的拉曼譜線。在實驗中我們發現,將少許的乙醇加入銀奈米顆粒中將會明顯提升銀奈米顆粒增強效應。在穿透式電子顯微鏡下,可觀察到我們自行製備的銀奈米顆粒大小約為十到一百奈米。我們所量測到的最低乳酸濃度為零點五莫爾濃度,這相當於人體中乳酸正常值的最低值。再這個濃度下針對特定的波峰做面積積分,其濃度與面積的相關係數可達零點九八四與零點九九六八。
ABSTRACT
Lactate, the byproduct of anaerobic glycolysis, has been applied to predict the disease severity for several clinical circumstances. Excessive accumulation of lactate will disrupt the acid-base equilibrium of physiological environment. In the literature, we applied surface enhanced Raman scattering (SERS), equipped by LabRAM HR-800 spectrophotometer, to measure lactate solution in physiological concentration. We also modified the standard preparation of silver colloid, and thus prevented the formation of anomalous bands in SERS generated during redox process. Incidentally, we found that the addition of small amount ethanol has promoted the enhancement efficiency of silver nanoparticle solution enormously. We have obtained better signals to noise ratio, and let the monitoring of lactate in physiological concentration become more possible.
The average size of self-assembly silver nanoparticle is ranged from 10nm to 100nm observed by transmission electron microscopy. The minimum concentration of lactate measured by us is 0.5 mM, the lowermost physiological level in human body. The correlation coefficient of area integration at 1385cm-1 in 0.5mM lactate is 0.9968. The other correlation coefficient of area integration at 840cm-1 in 0.5mM lactate is 0.984.
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3. A Zuger, "Cerebrospinal Fluid Lactate May
Predict Postoperative Meningitis," Journal Watch (1999).
5. Ellen J.Zeman and George C.Schatz, "An Acurrate Electromagnetic Theory Study of Surface Enhancement Factors for Ag, Au, Cu, Li, Na, Al, In, Zn, and Cd," J. Phys. Chem. 91, 634-643 (1987).
6. Hans-Peter Schuster, "Prognostic value of blood lactate in critically ill patients," Resuscitation 11, 141-146 (1984).
7. F. A. Husain, M. J. Martin, P. S. Mullenix, S. R. Steele, and D. C. Elliott, "Serum lactate and base deficit as predictors of mortality and morbidity," Am J. Surg. 185, 485-491 (2003).
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