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研究生:鐘緯儒
研究生(外文):Wei-Ju Chung
論文名稱:利用拉曼散射光譜測量色素上皮細胞層中黑色素的分布
論文名稱(外文):Melanin distribution in the porcine retinal pigment epithelium measured by Raman scattering spectroscopy
指導教授:張寅張寅引用關係
指導教授(外文):Yin Chang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生醫光電工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
中文關鍵詞:拉曼光譜黑色素視網膜色素上皮層
外文關鍵詞:Raman spectrummelaninRPE
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本研究的目的是想要知道視網膜色素上皮細胞層中的黑色素是否會干擾視網膜的扯曼光譜;且想要將黑色素定量,找出黑色素在視網膜組織裡的分布。黑色素的拉曼峰在155Ocm-1~160Ocm-1,利用豬眼睛當材料,得知色素上皮層在155Ocm-1~160Ocm-1也有拉曼峰。比較色素上皮層、鞏膜和色素上皮層覆蓋鞏膜這三種樣本的拉曼光譜,發現鞏膜並不會影響色素上皮層的訊號,另外兩種樣本在黑色素的拉曼波段有相同的光譜外形,但是強度上不一定一樣。我們發現豬眼睛色素上皮層不同位置的黑色素含量應該是相差不大,而個別豬眼睛黑色素含量在光譜強度上的差異大都在800a.u.附近。在黑色素是否會干擾視網膜的光譜實驗中,我們發現當視網膜尚未剝除,依然可以量到黑色素的訊號。當視網膜以正常狀態覆蓋於色素上皮層上時,黑色素拉曼強度跟沒有視網膜覆蓋時的黑色素拉曼強度一樣;若視網膜不是正常地覆於色素上皮層上,黑色素拉曼強度跟沒有視網膜覆蓋時的黑色素拉曼強度差別剖大。這應該是因為黑色素都聚集在色素上皮細胞的頂層,就包圍在感光細胞外節周圍,所以很容易就會收到黑色素的訊號。
The purpose of this study was to know whether the melanin in retinal pigment epithelium (RPE) would interfere with retina or not on Raman spectrum; to measure the contents of melanin and to determine the distribution of melanin in RPE. First of all, we tested the Raman spectrum of melanin powder which was synthesized. We got the range of spectrum of melanin powder from 1550cm-1 to 1600cm-1. We tried to measure the Raman spectrum of pig's RPE. The result of spectrum of pig's RPE had the same profile as melanin from 1550cm-1 to 1600cm-1, but the Raman intensity was not equal to melanin. We compared the signal of RPE, sclera, and RPE with sclera. We discover that the spectrum of sclera did not affect the signal of RPE, and we can see that the profile of RPE was the same as that of sclera. We found that the distribution of melanin in RPE didn't present regular model, and the difference of melanin contents was about 800 a.u. on Raman intensity. In the experiment about whether melanin would interfere the retina's Raman signal, we still catch the signal of melanin with and without retina covered on the RPE. The profiles of them were the same but the intensity was not. It would be due to the melanin accumulate at the apical part of RPE cell which surround with photoreceptor outer segment. Therefore, when we make laser light focused on the retina, melanin was easily in the tight focused area. Then we obtain the spectrum of melanin.
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