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研究生:張家綺
研究生(外文):Chia Ch''i Chang
論文名稱:大白鼠胚胎腦發育中GFAP與S-100免疫細胞化學之研究
論文名稱(外文):GFAP-like- and S-100-like- immunoreactivities in the rat brain during embryonic development
指導教授:游祥明
指導教授(外文):Shang Ming Yu
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:解剖暨細胞生物學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:73
中文關鍵詞:胚胎GFAPS-100免疫細胞化學
外文關鍵詞:brainembryoGFAPS-100immunocytochemistry
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在腦部受到傷害後,星狀膠細胞具有輔助神經細胞回復正常以及調控神經細胞和神經膠細胞的生成的功能。然而,星狀膠細胞會在受傷處形成堅韌的疤痕,阻礙腦神經重新連接的纏絡過程,進一步使得腦部的恢復減緩或停滯。反觀在胚胎腦發育過程中,星狀膠細胞或它的前祖細胞(放射神經膠細胞)與神經細胞共存,扮演著指引神經細胞遷徙、調節神經細胞生成的角色。因此本實驗主在利用glial fibrillary acidic protein (GFAP)及S-100蛋白的免疫細胞化學染色觀察鼠腦胚胎發育時期中(E14至E20),放射神經膠細胞變化成星狀膠細胞之時間與空間的變異情況並探討兩者之間的相互關聯性。GFAP-like免疫化學反應最早出現在E14天的大腦皮質與中腦蓋腦室區及延腦,而在基底核分化區最明顯;S-100-like免疫化學染色反應最早出現在E15天的橋腦腦室區,再往中腦、小腦及延腦後擴散。其在第四腦室周圍的腦室區都非常明顯。GFAP-like都比S-100-like免疫化學染色反應出現的早。S-100-like免疫化學染色反應集中在腦室區而GFAP-like免疫化學反應大多均勻地分散。本實驗提供大鼠胚胎發育時期第E14天到出生前(第E20天)放射神經膠細胞變化成星狀膠細胞之可能時程與其發育、分化、生長的關係,做為日後學者研究不同胚胎時期放射膠細胞或未成熟星狀膠細胞分化與環境調控因子關係之參考依據。
Astrocytes can directly or indirectly be involved in neurongenesis, gliogenesis, and neuron repairing after brain injuries. It can also slow recovery by forming a tough scar at the damaged area, which may form a barrier that keeps the brain from growing new connections. In contrast, astrocytes or the radial glia coexist with neuron and regulate the migration and synthesis in the embryonic brain development. This study had highlighted the spatial/temporal distribution of radial glia or early astroglia by GFAP- and S-100- immunoreactivities during embryonic brain differentiation and development (E14-E20). GFAP-like- immuno- reactivities was first observed in tegmentum, neocortex ventricular zone and myelencephalon at E14. Intense GFAP-like- immunoreactivities was in the basal ganglion differential zone; S-100-like- immnuoreactivities was firstly appeared in the ventricular zone of pons at E15 and then expanded to tegmentum, cerebellum, and myelencephalon. S-100-like- immnuoreactivities was very intense around forth ventricle. GFAP-like- immunoreactivities appeared earlier than S-100-like- immnuoreactivities in all area of brain. These results provide the early astroglia proliferation, differentiation and development with time course changes for further investigation about astroglial and its interaction with enviroment.
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