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研究生:李幸禧
研究生(外文):Hsing-His Li
論文名稱:運用果蠅為模式系統研究人類基因之生理功能
論文名稱(外文):Functional analysis of human genes using Drosophila as a model system
指導教授:廖國楨
指導教授(外文):Gwo-Jen Liaw
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:遺傳學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:英文
論文頁數:61
中文關鍵詞:果蠅細胞週期微管
外文關鍵詞:DrosophiladtlkKIAApumiliomitosismicrotubulecell cycle
相關次數:
  • 被引用被引用:0
  • 點閱點閱:112
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  • 下載下載:7
  • 收藏至我的研究室書目清單書目收藏:0
癌細胞是由於調控細胞增生、分化以及基因體完整性的基因突變
所引起,在後基因體時代,轉錄體 (transcriptomic)和蛋白質體
(proteomic),被運用來觀察正常細胞與癌化細胞中,基因表現量的差
異。在我研究的62 個基因中,大多在肝癌細胞中有大量的表現,其
中有三十個,也會在細胞週期的G2 至M 時期有高量表現。為了要研
究這些基因在正常細胞內的功能,我運用果蠅及其GAL4/UAS系統,
將這些基因於特定位置及時間大量表現,藉由分析其所表現之性狀,
推測其功能。
經由大規模的篩選之後,發現九個 (KIAA1102、KIAA0152、
KIAA0553、KIAA0295、FLJ11164、FLJ14450、FLJ13490、PEG10 和
PUMH2)會增強一目前已知和細胞週期調控有相關之基因- dtlk的性
狀,在其中,我挑選PUMH2 做更進一步的研究,除了已知的功能是
參與轉譯抑制子,由目前的研究及我的實驗結果顯示, dtlk 可能透
過第三因子與PUMH2 進行相互作用,並作用於細胞分裂週期之不同
時期,影響細胞內微小管(Microtubules)的動態平衡,進而影響到細
胞分裂週期的進行。
Cancer development is due to mutations in multiple genes involved in cell proliferation, differentiation, apoptosis and genomic integrity. In the post-genomic era, the transcriptomic and proteomic analyses are used to determine gene expression profiles of certain cell types. Genes, upregulated in a certain cell types including cancer cells, can be easily identified. In my study, most genes, upregulated in hepatoma cells, were found by such ways. In collaboration with other fly labs, transgenic flies carrying 62 different human genes were established (at lease 6 lines per construct). My contribution is to make 26 constructs.
After analyzing the lines, I found nine dtlk enhancers (KIAA1102, KIAA0152, KIAA0553, KIAA0295, FLJ11164, FLJ14450, FLJ13490, PEG10, and PUMH2) using the GAL4/UAS system. Currently, I have focused on PUMH2. The results indicate that PUMH2 antagonizes DTLK at the G2/M transition, which partly through regulate the dynamic
changes of microtubules. This is consistent with the notion that the dynamic change of microtubules is crucial for the progression of cell cycle.
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