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研究生:洪啟盛
研究生(外文):Qi-Sheng Hong
論文名稱:篩選與特性描述會和Eyg結合的蛋白質
論文名稱(外文):Identification and Characterization of Proteins Interacting with Eyg
指導教授:孫以瀚孫以瀚引用關係
指導教授(外文):Y. Henry Sun
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:遺傳學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:英文
中文關鍵詞:果蠅眼睛發育
外文關鍵詞:Drosophilaeye goneyeasy two hybridebismrsnoeygeye development
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eyg gone (eyg)在果蠅眼睛發育上扮演一個相當重要的角色。當果蠅的eyg基因發生突變時,會造成果蠅眼睛變小或完全消失;相反地,當利用dpp-GAL4異位表現eyg時,則可以在果蠅正常眼睛的腹側誘發一獨立的小眼睛。雖然eyg對果蠅眼睛調控很重要,對於eyg調控眼睛的機制卻不甚了解。本論文藉由尋找和Eyg結合的蛋白質來探討eyg調控眼睛發育的可能機制。
eyg基因屬於Pax家族的一員。我們利用yeast two-hybrid的方法在果蠅胚胎表現的蛋白質中尋找可能會和Eyg結合的蛋白。eyg可以轉譯出一含不完整paired domain和完整homeodomain的蛋白質,這兩個domain在演化上都是相當保留的,若是以這兩個domain來當做bait可能會找到一些對Eyg並不具有專一性的蛋白。因此我們選用在這兩個domain中間linker和C端的部位當作bait來尋找和Eyg能直接作用的蛋白質。
在許多可能的蛋白中,經由近一步的檢驗,我們發現一個具有co-repressor作用的基因—ebi,當ebi一個基因發生突變時會使得eyg突變株(eyg1/eygM3-12)在蛹期死亡,並且發現ebi是藉由C端的部位來和Eyg作用。進一步發現這個co-repressor complex其他成員(Smr和Sno)的突變也會增加eyg突變株(eyg1/eygM3-12)在蛹期的死亡率,顯示Eyg可能藉由此co-repressor complex來執行他的功能。
As a member of Pax family, eyegone encodes a truncated paired domain and a homeodomain implicated in eye development. Loss-of-function mutations in eyg cause a reduced or eyeless phenotype, while ectopic expression of eyg using the dpp-GAL4 driver produces eye formation on the ventral regions of the adult head.
eyg plays a significant role in eye development, but there is little known about the mechanism of eyg function. In order to study what pathway regulating eye development eyg may be involved in, it is very important to identify what proteins interact with Eyg protein. I used the yeast two-hybrid system to ascertain physical protein-protein interaction.
Eyg has the conserved paired and homeobox domains, plus the linker region and C-terminal domain. In domain deletion and swap experiments, our lab has found that the linker B region and C-terminal domain may play some roles, perhaps in protein-protein interaction. So I used these two domains as baits to identify the proteins physically interacting with Eyg.
I showed that the F-box / WD40 repeats protein Ebi interacts with Eyg by the C-terminal domain, and loss-of-function mutation in ebi will increase the pupa lethality of eyg (eyg1/eygM3-12) mutant.
Bonini, N. M., Leiserson, W. M., and Benzer, S. (1993). The eyes absent gene: genetic control of cell survival and differentiation in the developing Drosophila eye. Cell 72, 379-395.
Brent, R., and Ptashne, M. (1985). A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell 43, 729-736.
Czerny, T., Halder, G., Kloter, U., Souabni, A., Gehring, W. J., and Busslinger, M. (1999). twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development. Mol Cell 3, 297-307.
Czerny, T., Schaffner, G., and Busslinger, M. (1993). DNA sequence recognition by Pax proteins: bipartite structure of the paired domain and its binding site. Genes Dev 7, 2048-2061.
Dong, X., Tsuda, L., Zavitz, K. H., Lin, M., Li, S., Carthew, R. W., and Zipursky, S. L. (1999). ebi regulates epidermal growth factor receptor signaling pathways in Drosophila. Genes Dev 13, 954-965.
Eberl, D. F., and Hilliker, A. J. (1988). Characterization of X-linked recessive lethal mutations affecting embryonic morphogenesis in Drosophila melanogaster. Genetics 118, 109-120.
Emmons, S., Phan, H., Calley, J., Chen, W., James, B., and Manseau, L. (1995). Cappuccino, a Drosophila maternal effect gene required for polarity of the egg and embryo, is related to the vertebrate limb deformity locus. Genes Dev 9, 2482-2494.
Erickson, J. W., and Cline, T. W. (1993). A bZIP protein, sisterless-a, collaborates with bHLH transcription factors early in Drosophila development to determine sex. Genes Dev 7, 1688-1702.
Fischbach, K. F. (1983). Neural cell types surviving congenital sensory deprivation in the optic lobes of Drosophila melanogaster. Dev Biol 95, 1-18.
Fischbach, K. F., and Technau, G. (1984). Cell degeneration in the developing optic lobes of the sine oculis and small-optic-lobes mutants of Drosophila melanogaster. Dev Biol 104, 219-239.
Giot, L., Bader, J. S., Brouwer, C., Chaudhuri, A., Kuang, B., Li, Y., Hao, Y. L., Ooi, C. E., Godwin, B., Vitols, E., et al. (2003). A protein interaction map of Drosophila melanogaster. Science 302, 1727-1736.
Guenther, M. G., Lane, W. S., Fischle, W., Verdin, E., Lazar, M. A., and Shiekhattar, R. (2000). A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness. Genes Dev 14, 1048-1057.
Halder, G., Callaerts, P., and Gehring, W. J. (1995). New perspectives on eye evolution. Curr Opin Genet Dev 5, 602-609.
Hershko, A., and Ciechanover, A. (1998). The ubiquitin system. Annu Rev Biochem 67, 425-479.
Hope, I. A., and Struhl, K. (1986). Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast. Cell 46, 885-894.
Jang, C. C., Chao, J. L., Jones, N., Yao, L. C., Bessarab, D. A., Kuo, Y. M., Jun, S., Desplan, C., Beckendorf, S. K., and Sun, Y. H. (2003). Two Pax genes, eye gone and eyeless, act cooperatively in promoting Drosophila eye development. Development 130, 2939-2951.
Jones, N. A., Kuo, Y. M., Sun, Y. H., and Beckendorf, S. K. (1998). The Drosophila Pax gene eye gone is required for embryonic salivary duct development. Development 125, 4163-4174.
Jun, S., and Desplan, C. (1996). Cooperative interactions between paired domain and homeodomain. Development 122, 2639-2650.
Jun, S., Wallen, R. V., Goriely, A., Kalionis, B., and Desplan, C. (1998). Lune/eye gone, a Pax-like protein, uses a partial paired domain and a homeodomain for DNA recognition. Proc Natl Acad Sci U S A 95, 13720-13725.
Keegan, L., Gill, G., and Ptashne, M. (1986). Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science 231, 699-704.
Lane, M. E., Elend, M., Heidmann, D., Herr, A., Marzodko, S., Herzig, A., and Lehner, C. F. (2000). A screen for modifiers of cyclin E function in Drosophila melanogaster identifies Cdk2 mutations, revealing the insignificance of putative phosphorylation sites in Cdk2. Genetics 155, 233-244.
Mardon, G., Solomon, N. M., and Rubin, G. M. (1994). dachshund encodes a nuclear protein required for normal eye and leg development in Drosophila. Development 120, 3473-3486.
Ostberg, T., Jacobsson, M., Attersand, A., Mata de Urquiza, A., and Jendeberg, L. (2003). A triple mutant of the Drosophila ERR confers ligand-induced suppression of activity. Biochemistry 42, 6427-6435.
Pignoni, F., Hu, B., and Zipursky, S. L. (1997). Identification of genes required for Drosophila eye development using a phenotypic enhancer-trap. Proc Natl Acad Sci U S A 94, 9220-9225.
Pile, L. A., and Wassarman, D. A. (2000). Chromosomal localization links the SIN3-RPD3 complex to the regulation of chromatin condensation, histone acetylation and gene expression. Embo J 19, 6131-6140.
Quiring, R., Walldorf, U., Kloter, U., and Gehring, W. J. (1994). Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans. Science 265, 785-789.
Scheffner, M., Nuber, U., and Huibregtse, J. M. (1995). Protein ubiquitination involving an E1-E2-E3 enzyme ubiquitin thioester cascade. Nature 373, 81-83.
Treisman, J., Harris, E., and Desplan, C. (1991). The paired box encodes a second DNA-binding domain in the paired homeo domain protein. Genes Dev 5, 594-604.
Treisman, J. E. (1999). A conserved blueprint for the eye? Bioessays 21, 843-850.
Tsai, C. C., Kao, H. Y., Yao, T. P., McKeown, M., and Evans, R. M. (1999). SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development. Mol Cell 4, 175-186.
Tsuda, L., Nagaraj, R., Zipursky, S. L., and Banerjee, U. (2002). An EGFR/Ebi/Sno pathway promotes delta expression by inactivating Su(H)/SMRTER repression during inductive notch signaling. Cell 110, 625-637.
Wilson, D., Sheng, G., Lecuit, T., Dostatni, N., and Desplan, C. (1993). Cooperative dimerization of paired class homeo domains on DNA. Genes Dev 7, 2120-2134.
Wilson, D. S., Guenther, B., Desplan, C., and Kuriyan, J. (1995). High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA. Cell 82, 709-719.
Wolff, T. a. R., D. F. (1993). Pattern formation in the Drosophila retina. Cold Spring Harb Laboratory, Plainview, NY, 1277-1326.
Xu, H. E., Rould, M. A., Xu, W., Epstein, J. A., Maas, R. L., and Pabo, C. O. (1999). Crystal structure of the human Pax6 paired domain-DNA complex reveals specific roles for the linker region and carboxy-terminal subdomain in DNA binding. Genes Dev 13, 1263-1275.
Zhang, K., Smouse, D., and Perrimon, N. (1991). The crooked neck gene of Drosophila contains a motif found in a family of yeast cell cycle genes. Genes Dev 5, 1080-1091.
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