臺灣博碩士論文加值系統

本研究改良先前學者之合成方法，合成核心為硒化鎘外層包覆硫化鋅的親水性奈米晶體(又稱為量子點)。以表面修飾帶有羧基(carboxyl groups，-COOH) 的量子點，其螢光為綠色及紅色，發射光譜分別為524.4 nm及635 nm，extinction coefficient依序為5.96 × 104和5.63 × 105來標定膠原蛋白纖維。以螢光強度的明顯增加間接證實硒化鎘外層確實包覆上硫化鋅而形成core/shell結構。利用核磁共振光譜儀分析，證實量子點成功地羧基化而變為親水性。利用EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) 交聯標定膠原蛋白纖維。以量子點改進傳統作為細胞標定的螢光染劑受限於其半衰期過短，僅能用特定波長激發及發出單一色光之缺點。由螢光強度衰逝測試，發現以量子點標定膠原蛋白纖維螢光強度不會隨連續激發而大幅衰減。在穿透式電子顯微鏡下觀察量子點於膠原蛋白纖維上附著的情形，統計其標定的位置。以觀察的方式直接找出膠原蛋白上標定位置，發現在特定的位置上確有較多量子點的附著，將其與膠原蛋白的胺基酸序列對照，發現這些位置確為帶電基團較密集處且皆有lysine的存在。使用不同螢光顏色的量子點標定膠原蛋白纖維，最後將雙色螢光標定的膠原蛋白纖維等量混合，作為將來細胞培養用基材。

My research modifies former scholars’ synthesis methods, synthesizing water-soluble CdSe/ZnS (core/shell) quantum dots (QDs). We label collagen fibers with carboxyl quantum dots, whose colors are green and red, emission wavelengths are 524.4 nm and 635 nm, and extinction coefficients are 5.96 × 104 and 5.63 × 105 respectively. Apparent increase in fluorescent intensity indirectly proves that CdSe QDs are overcoated with ZnS to form core/shell structure. We use NMR to analyze carboxyl QDs, and confirm that QDs are successfully carboxylized to become water-soluble. We use carboxyl QDs to label collagen fibers crosslinked by EDC (1-ethyl-3- (3-dimethylaminopropyl) carbodiimide). We use QDs to improve the limitations of fluorescent dyes for cell-labeling because of their short half-time, narrow excitation wavelength and single color emission. From fluorescent intensity decay test, we find that the fluorescent intensity of QD-labeled collagen fibers won’t remarkably decay under successive irradiation. Under transmission electron microscope, we can observe the binding situation of QDs on collagen fibers, and estimate the QDs binding on specific locations. We directly observe QDs on collagen fibers, and discover that there are more QDs labeled on specific locations. Compared with the amino acid sequence of collagen, we see that these locations are densely charged regions and also find the existence of lysine. At last, we mix equal volume of dual-color labeling collagen fibers to form a matrix for cell culture.

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尹邦躍編著 奈米時代 五南書局 2002
黃國瑋 懸浮性II-VI族化合物半導體奈米粒子之合成與鑑定 台灣大學化學研究所 碩士論文 2003