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研究生:丁菱
研究生(外文):Ling Ting
論文名稱:應用Th1/Th2基因轉殖小鼠對台灣株日本血吸蟲26KDaGST抗原免疫調控機轉及免疫保護力之研究
論文名稱(外文):The protective immunity and immunoregulatory mechanisms of 26 KDa GST antigen against Schistosoma japonicum(Taiwanese strain)in Th1/Th2 transgenic mice model
指導教授:李金木李金木引用關係
指導教授(外文):Kin-Mu Lee
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:寄生蟲學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:106
中文關鍵詞:日本血吸蟲GSTTh1/Th2基因轉殖小鼠磁石分離技術流式細胞儀
外文關鍵詞:Schistosoma japonicumGSTTh1/Th2 transgenic miceMACSFlow cytometry
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在血吸蟲疫苗的發展上,麩胺基硫S-轉移酶(Glutathione-S-
transferases;GST)為目前公認主要的候選抗原之一。本研究論文應用Th1/Th2基因轉殖小鼠模式進一步探討台灣株日本血吸蟲26 KDa rGST疫苗抗原在宿主體內的免疫調控機轉,同時配合磁石分離技術純化CD4+ T細胞及B細胞進行被動轉移試驗探討其免疫保護機轉。研究結果顯示,小鼠經SjT 26 KD rGST免疫後,其體內可產生具高度專一性的IgG及IgG2b之高抗體力價,在感染台灣株日本血吸蟲後,其體內所產生的CD4+ T細胞在第五週有明顯上升;而CD8+ T細胞在整個實驗過程中沒有明顯的變化趨勢。CD4+ 中Th1細胞在感染後第一週起有明顯的上升;而Th2細胞則在感染慢性期第十二週開始明顯上升。B細胞在感染第四、五及十二週有大量表現之情形;B細胞中之Be1在感染初期至急性期都有增加。NK細胞在感染急性期有表現較明顯之趨勢;NK細胞中NK1細胞在第五週增加表現最為明顯;NK2細胞則在慢性期有趨於上升之表現反應。CD4+ T細胞及B細胞免疫保護力測試結果顯示經由SjT 26 KD rGST免疫誘發所產生之淋巴細胞可引起相當程度的免疫保護功效。
Glutathione-S-transferase (GST)is generally recognized as one of the major candidate vaccine antigen. The purpose of this study is to use a transgenic mice model to further investigate the immunoregulatory mechanisms and protective immunity brought about by the 26 KDa GST vaccine antigen from a Taiwanese strain of Schistosoma japonicum. The adoptive immunity induced were investigated with CD4+ T and B cells purified by MACS technique. The results showed that anti 26 KDa GST total IgG and IgG2b antibodies in immunized mice were significantly higher than those in control mice. The CD4+ T cells increased significantly at wk 5 after infection, whereas, the CD8+ T cells showed no statistically significant differences during the experiment. The Th1 subset of CD4+ T cells began to increase at wk 1 while the Th2 subset CD4+ T cells began to rise at wk 12. B cells increased markedly and reached a maximum at wk 4、5 and 12. The Be1 subset of B cells increased significantly from early to acute stages. NK cells began to rise at acute stages. The NK1 subset of NK cells increased significantly at wk 5 while the NK2 subset of NK cells started to increase at chronic stages. The results of adoptive immunity indicated that CD4+ T and B cells induced by SjT 26 KDa rGST could confer a significant protection by reduction in worm burdens.
李貞瑩. 1999. 大陸株日本血吸蟲病細胞激素基因免疫調節之分析. 國立陽明大學碩士論文.
柳舒祥. 1996. 台灣株日本血吸蟲26 KDa麩胺基酸硫S-轉移酶基因之分子選殖及疫苗特性之研究. 國立陽明大學碩士論文.
陳念榮. 2000. 第一與第二型輔助型T細胞表面抗原之研究. 國立陽明大學博士論文.
黃志傑. 2001. 日本血吸蟲26 KDa GST疫苗抗原被動免疫分析及應用鋁佐劑誘導免疫效應之研究. 國立陽明大學碩士論文.
黃桂芬. 2000. 應用基因轉殖小鼠對日本血吸蟲GST疫苗抗原免疫調節機轉及其免疫病理之分析研究. 國立陽明大學碩士論文.
楊明芬. 1999. 以IL-12作為26 KDa麩胺基酸硫S-轉移酶疫苗佐劑抗日本血吸蟲之免疫效應. 國立陽明大學碩士論文.
劉吟芬. 2003. 應用基因轉殖小鼠對曼氏血吸蟲病Th1/Th2細胞免疫調節機轉之研究. 國立陽明大學碩士論文.
盧珍妏. 1998. 台灣株日本血吸蟲病Th1及Th2細胞免疫調節之分析研究. 國立陽明大學碩士論文.
羅進宗. 1990. 彰化縣日本血吸蟲疫區住民、學童及大陸探親榮民之寄生蟲調查. 中華民國寄生蟲學雜誌 3:97-100.
Acosra LP., McManus DP., Aligui GD., Olveda RM. and Tiu WU. 2004. Antiget-specific antibody isotype patterns to Schistosoma japonicum recombinant and native antigens in a defined population in Leyte, the Philippines. American Journal of Tropical Medicine & Hygiene 70(5):549-55.
Adrian P. Mountford, Sonia Anderson and R. Alan Wilson. 1996. Induction of Th1 cell-mediated protective immunity to Schistosoma mansoni by Co-Administration of larval antigens and IL-12 as an Adjuvant. The Journal of Immunology 156:4739-4745.
Akram A. Da’dara, Patrick J. Skelly, MaoMao Wang, Donald A. Harn. 2002. Immunization with plasmid DNA encoding the integral membrane protein, Sm23, elicits a protective immune response against schistosome infection in mice. Vaccine 20:359-369.
Chen H., Nara T., Zeng X., Satoh M., Wu G., Jiang w., Yi F., Kojima S., Zhang S. and Hirayama K. 2000. Vaccination of domestic pig with recombinant paramyosin against Schistosoma japonicum in China. Vaccine 18:2142-2146.
Dario AAV, Quentic DB and Martin GT. 1989. Immunity to Schistosoma mansoni in vivo:contradiction or clarification? Immmunology Today 10:410-416.
Dresden M.H. and Payne D.C. 1981. A sieving method for the collection of Schistosome eggs from mouse intestines. Journal of Parasitology 67(3):450-52.
Duvall R.H. and DeWitt W.B. 1967. An improved perfusion technique for recovering adult schistosomes from laboratory animals. The American Journal of Tropical Medicine and Hygiene 16:483-486.
Fan, P. C., K. M. Lee, C. C. Wu and C. Y. Liu. 1994. Further study on infectivity of zoophilic and anthropophilic strains of Schistosoma japonicum to different races of Oncomelania Hupensis Snails. Yonsei Reports on Tropical Medicine 25:23-29.
Fuhui S., Zhang Y., Ye P., Lin J., Cai Y., Shen W., Bickle Q.D. and Taylor M.G. 2002. Laboratory and field evaluation of Schistosoma japonicum DNA vaccines in sheep and water buffalo in China. Vaccine 20:462-467.
Gaur S., Kesarwala H., Gavai M. and Gupta M. 1994. Clinical Immunology and Infectious Disease. Pediatric Clinics of North America 41(4):745-778.
Geoffrey N. Gobert. 1998. Immunolocalization of Schistosome Proteins. Microscopy Research And Technique 42:176-185.
Gobert G.N., Stenzel D.J. and McManus D.P. 1998. Immunolocalisation of the glutathions S-transferases, GST-26 and GST-28, within adult Schistosoma japonicum. 1998. International Journal for Parasitology 28:1437-1443.
Grzych JM., Pearce E., Cheever A., et al. 1991. Egg deposition is the stimulus for the production of TH2 cytokines in murine Schistosomiasis mansoni. Journal of Immunology 146:1322-40.
Harris DP, Haynes L, Sayles PC, Duso DK, Eaton SM, Lepak NM, Johnson LL, Swain SL and Lund FE. 2000. Reciprocal regulatopn of polarized cytokine production by effector B and T cells. Nature 475-482.
Hernandez HJ, Wang Y and Stadecker MJ. 1997. In infection with Schistosoma mansoni, B cells are required for T with helper type 2 cell responses but not for granuloma formation. Journal of Immunology 158:4832-4837.
Hirata M., Nakashima T. and Fukuma T. 1999. Characteristic of eosinophilic inclusions within Schistosoma japonicum eggs. Parasitology 118:269-274.
Hsieh SL, Chen NJ, Liao NS, Lai YG, Lee KM, Lee KH, Wu SC, Han SH and McDevitt H. 2000. Transgenic mice expressing surface markers for Th1 and Th2 cells. Molcular Immunology 37:281-293.
Hsu CK. and Hsu Li SY. 1956. On the infectivity of the Formosan strain of Schistosoma japonicum in Homo sapiens. American Journal of Tropical Medicine and Hygiene 12:521-528.
Hsu CK. and Hsu Li SY. 1962. Schistosoma japonicum in Formosa:A critical review. Experimental parasitology 12:459-462.
Ingrid Schmid, Mirabelle D. Dagarag, Mary Ann Hausner, Jose L. Matud, Tom Just, Rita B. Effros, and Beth D. Jamieson. 2002. Simultaneous flow cytometric analysis of two cell surface markers, telomere length, and DNA content. Cytometry 49:96-105.
Jie Liu, Kachio Tasaka, Junqi Yang, Takehito Itoh, Muneo Yamada, Hideshi Yoshikawa and Yasuo Nakajima. 2001. Identification of a novel T-Cell epitope in soluble egg antigen of Schistosoma japonicum. Infection and Immunity 69(6):4154-4158.
Jinchun Zhou, Gary J. Waine, Qingren Zeng, Xianfang Zeng, Xinyuan Yi and Donald P. McManus. 1999. B-cell epitopes recognized by Chinese water buffaloes (Bos buffelus) on the 22 kDa tegumental membrane-associated antigen (Sj-22) of the Asiatic bloodfluke, Schistosoma japonicum. Veterinary Research 30:427-432.
Julin C. Scott and Donald P. McManus. 2000. Molecular cloning and enzymatic expression of the 28-kDa glutathione S-transferase of Schistosoma japonicum : evidence for sequence variation but lack of consistent vaccine efficacy in the murine host. Parasitology International 49:289-300.
Jun-Qi Yang, Kachio Tasaka, Cheng-Kuo Chuang, Hideshi Yoshikawa and Yasuo Nakajima. 1999. Characterization of murine Th1 clones specific to egg antigen of Schistosoma japonicum and their interaction with cytokine. Parasite Immunology 21(11):583-593.
Lee, K. M., C. T. Lo, P. C. Fan and C. K. Chung. 1992. Susceptibility on Oncomelania hupensis subspecies to infection with Chinese, Taiwan and Japanese strains of Schistosoma japonicum. Chinese Journal of Parasitology 5:67-71.
Lesley E., et al. 1996. Kinetics and mechanism of effector focus formation in the lungs of mice vaccinated with irradiated cercariae of Schistosoma mansoni. Parasite Immunology 18(7):359-69
Liu Shuxian, He Yongkang, Sing Guangchen, Luo Xing-song, Xu Yuxin and Donald P. McManus. 1997. Anti-fecundity immunity to Schistosoma japonicum induced in Chinese water buffaloes (Bos buffelus) after vaccination with recombinant 26 kDa glutathione-S-transferase (reSjc26GST). Veterinary Paraitology 69:39-47.
Liu S.X., Song G.C., Xu Y.X., Yang w. and McManus D. P. 1995. Anti-fecundity immunity induced in pigs vaccinated with recombine-
ant Schistosoma japonicum 26kDa glutathione-S-transferase. Parasite Immunology 17(7):335-340.
Liu S.X., Song G.C., Xu Y. X., Yang w. and McManus D. P. 1995. Immunization of mice with recombinant Sjc26GST induces a pronounced anti-fecundity effect after experimental infection with Chinese Schistosoma japonicum. Vaccine 13(6):603-607.
Loza MJ and Perussia B. 2001. Final steps of natural killer cell maturation:a model for type 1-type 2 differentiation? Nature Immunology 2(10):917-924.
McManus D.P., Wong J.Y.M., Zhou J., Cai C., Zeng Q., Smyth D., Li Y., Kalinna B.H., Duke M.J. and Yi X. 2002. Recombinant paramyosin (rec-Sj-97) tested for immunogenicity and vaccine efficacy against Schistosoma japonicum in mice and water buffaloes. Vaccine 20:870-878.
Osada Y., Tuenta Janecharut, Hata H., Yuvadee Mahakunkij-Charoen, Chen X.-w., Nara T., Kita K. and Kojima S. 2001. Protective immunity to Schistosoma japonicum infection depends on the balance of T helper cytokine reponses in mice vaccinated with γ-irradiated cercariae. Parasite Immunology 23(5):251-258.
Peritt D, Robertson S, Gri G, Showe L, Aste-Amezaga M and Trinchieri G. 1998. Differentiation of Human NK cells into NK1 and NK2 Subets. The Journal of Parasitology 161:5821-582.
Ross A.G.P., Sleigh A.C., Li Y.S., Williams G.M., Aligui G.D.L. and McManus D.P. 2000. Is there Immunity to schistosoma japonicum? Parasitology Today 16(4):159-164.
Runhua Hou, Olga Goloubeva, Donna S. Neuberg, Jack L. Strominger and S. Brian Wilson. 2003. Interleukin-12 and interleukin-2-induced invariant natural killer T-cell cytokine secretion and perforin expression independent of T-cell receptor action. Immunology 110:30-37.
Schmid I., Dagarag M.D., Hausner M.A., Matud J.L., Just T., Effros R.B. and Jamieson B.D. 2002. Simultaneous flow cytometric analysis of two cell surface markers, telomere length, and DNA content. Cytometry 49:96-105.
Serge Mostowy, Anthony G. Tsolaki, Peter M. Small and Marcel A. Behr. 2003. The in vitro evolution of BCG vaccines. Vaccine 21:4270-4274.
Shenghua Zhou, Shuxian Lin, Guangcheng Song, Yuxin Xu, Wenyu Sun. 2000. Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese Schistosoma japonicum. Vaccnie 18:3196-3204.
Shi F., Zhang Y., Lin J., Zuo X., Shen W., Cai Y., Ye P., Bickle Q.D. and Taylor M.G. 2002. Field testing of Schistosoma japonicum DNA vaccines in cattle in China. Vaccine 20:3629-3631.
Simone Korten, Lars Volkmann, Michael Saeftel et al., 2002. Expansion of NK cells with reduction of their inhibitory Ly-49C, and Ly-49G2 receptor-expressing subsets in a murine helminth infection:contribution to parasite control. The Journal of Immunology 168:5199-5206.
Smith, D. B., K. M. Davern, P. G. Board, W. U. Tiu, E. G. Garcia and G. F. Mitchell. 1986. Mr 26000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proceeding of National Academy of Science USA. 83:8703-8707.
Subramonia Pillai, Kathy Dermody and Ben Metcalf. 1995. Immunogenicity of genetically engineered Glutathione S-Transferase fusion proteins containing a T-cell epitope from diphtheria toxin. Infection and Immunity 63(4):1535-1540.
Takeshi Nara, Kazuyuki Tanabe, Yuvadee Mahakunkijcharoen, Yoshio
Osada, Naoki Matsumoto, Kiyoshi Kita and Somei Kojima. 1997. The B cell epitope of paramyosin recognized by protective monoclonal IgE antibody to Schistosoma japonicum. Vaccine 15(1):79-84.
Taylor M.G., Huggins M.C., Fuhui S., Lin J., Tian E., Ye P., Shen w., Qian C.G., Lin B.F. and Bickle Q.D. 1998. Production and testing of Schistosoma japonicum candidate vaccine antigens in the natural ovine host. Vaccine 16(13):1290-1298.
Thomas A. Wynn, Dragana Jankovic, Sara Hieny, allen W. Cheever and Alan Sher. 1995. IL-12 enhance vaccine-induced immunity to Schistosoma mansoni in mice and decreases T helper 2 cytokine expression, IgE production, and tissue eosinophilia. The Journal of Immunology 154:4701-4709.
Thomas A. Wynn, Alicia Reynolds, Stephanie James, Allen W. Cheever, Pat Caspar, Sara Hieny, Dragana Jankovic, Mette Strand and Alan Sher. 1996. IL-12 enhances vaccine-induced immunity to Schistosomes by augmenting both humoral and cell-mediated immune responses against the parasite. The Journal of Immunology 157:4068-4078.
Varley C.A., Dunne D.W. and Havercroft J.C. 1992. The influence of adjuvant on humoral responses to glutathione-S-transferase fusion proteins. Parasite Immunology 14:557-562.
Wynn T.A. and Hoffmann K.F. 2000. Defining a Schistosomiasis vaccination strategy-Is it really Th1 versus Th2? Parasitology Today 16(11):497-501.
Yaobi Zhang, Martin G. Taylor, Maria V. Johansen and Quentin D. Bickle. 2002. Vaccination of mice with a cocktail DNA vaccine induces a Th1-type immune response and partial protection against Schistosoma japonicum infection. Vaccine 20:724-730.
Yi-Xun He, Buz Salafsky and Kalyanasundaram Ramaswamy. 2001. Host-parasite relationships of Schistosoma japonicum in mammalian hists. TRENDS in Parasitology 17(7):320-324.
Yong zhan, Xi Song and G. Wayne Zhou. 2001. Structural analysis of regulatory protein domains using GST-fusion proteins. Gene 281:1-9.
Yuesheng Li, Alyson Auliff, Malcolm K.Jones, Xinyuan Yi and Donald P.McManus. 2000. Immunogenicitiy and immunolocalization of the 22.6 kDa antigen of Schistosoma japonicum. Parasite Immunology 22(8):415-424.
Zhang L., Mi J., Yu Y., Yao H., Chen H., Li M. and Cao X. 2001. IFN-γ gene therapy by intrasplenic hepatocyte transplantation:a novel strategy for reversing hepatic fibrosis in Schistosoma japonicum-infected mice. Parasite Immunology 23(1):11-17.
Zhang R., Yoshida A., Kumagai T., Kawaguchi H., Maruyama H., Suzuki T., Itoh M., EL-Malky M. and Ohta N. 2001. Vaccination with Calpain Induces a Th1-Biased Protective Immune Response against Schistosoma japonicum. Infection and Immunity 69(1):386-391.
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