臺灣博碩士論文加值系統

基因治療是指利用基因工程將去氧核醣核酸送入宿主細胞，取代異常的基因，恢復正常的生理弁遄C基因治療的過程可分為供給、運送及表達三種步驟。供給方面包括ex vivo 、in situ 、in vivo三種方式。運送基因的載體通常分為病毒性的載體及非病毒性的載體。本實驗著重的主題即是要探討載體運送和表達的能力。主要利用人類多瘤性病毒，JCV，的主要殼體蛋白，VP1，自行組裝成的類病毒殼體(virus-like particle；VLP)，當做一種基因治療的基因運送載體，試圖了解此載體能否攜帶綠色螢光蛋白基因，並在多種腫瘤組織中表達此基因的弁遄C實驗結果顯示人類多瘤性病毒，JCV，組裝成的類病毒殼體，可以攜帶綠色螢光蛋白基因，並在肺腺癌腫瘤組織、直腸腺癌腫瘤組織中表達綠色螢光蛋白基因。而人類多瘤性病毒，JCV，類病毒殼體，除了能在人類直腸腺腫瘤SW620腫瘤組織中轉達綠色螢光蛋白基因，也可以在裸鼠的肝臟轉達綠色螢光蛋白基因。因此，人類多瘤性病毒，JCV，類病毒殼體，除了能在腫瘤部位轉達綠色螢光蛋白基因外，也能在正常的組織器官中轉達綠色螢光蛋白基因。或部A在未來人類基因治療上，人類多瘤性病毒，JCV，類病毒殼體能發展為不錯的基因運送載體。

Gene therapy refers sending deoxyribonucleic acids to the host cell by using genetic engineering, to substitute the abnormal genes and restore physiological functions. Gene therapy may divide into three steps that are supply, delivery and expression. Supply includes “ex vivo”,“in situ”and “in vivo”three ways. Delivery vectors usually include viral carrier and non-viral carrier. This study focuses on the gene transfer ability and transduction efficiency using the JCV VLP as a gene delivery vector. The major capsid protein, VP1, of the human polyomavirus, JCV, that can self-assemble into virus-like particle (VLP). We attempted to understand whether this vector could carry foreign gene (green fluorescence protein gene) and express the gene in various kinds of human tumors. The results showed that JCV VP1 VLP could carry and transduce green fluorescence protein (GFP) gene into lung adenocarcinoma and colon adenocarcinoma. Not only did JCV VP1 VLP transduce the green fluorescence protein gene in metastatic colorectal adenocarcinoma (SW620), but also transduce the gene into the liver of nude mice. However, JCV VP1 VLP may potentially be developed as a gene delivery vector for human gene therapy in the future.

國立中正大學分子生物研究所碩士論文 I
誌謝 II
中文摘要 III
英文摘要 IV
目錄 V
細目錄 VI
圖目錄 IX
表目錄 XIII
第一章 緒論 1
第二章 材料與方法 21
第三章 結果 32
第四章 討論 37
第五章 實驗結果圖表 40
參考文獻 92
附圖、表 100

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