臺灣博碩士論文加值系統

許多神經退化性疾病如阿茲海默症等，與長時間累積氧化壓力造成神經細胞的損傷有關。因此有學者建議以抗氧化藥物作為神經退化性疾病之預防及治療。女貞子為木犀科植物女貞之成熟果實。本研究首先進行女貞子、墨旱蓮及二至丸（女貞子、墨旱蓮各等份所組成）之抗氧化成分含量與活性評估，其次並以離體方式探討女貞子對H2O2誘導SH-SY5Y細胞氧化壓力而致神經細胞損傷之神經保護作用及作用機轉。
在抗氧化成分暨活性分析之研究結果發現，二至丸較女貞子及墨旱蓮具有較佳之抗氧化活性。女貞子粗抽物有抗氧化暨抗脂質過氧化等作用，且對超氧陰離子、氫氧自由基及脂自由基等均具有清除作用，而女貞子粗抽物經分析含有總酚類、類黃酮及鞣質等抗氧化成分。而在各分層中，抗氧化及超氧陰離子清除活性以乙酸乙酯層較佳；但在氫氧自由基及脂自由基清除活性則以氯仿層較佳。
其次，在H2O2誘導SH-SY5Y細胞氧化損傷之研究結果發現，女貞子粗抽物對H2O2誘導SH-SY5Y細胞氧化損傷具保護作用，且可降低細胞內氧化壓力及反轉因H2O2導致 GSH 含量暨GSH/GSSG比值減少之現象。而女貞子乙酸乙酯層及氯仿層亦可保護H2O2誘導SH-SY5Y細胞氧化損傷，其中以乙酸乙酯層較佳；同時乙酸乙酯層亦可降低細胞內氧化壓力、反轉因H2O2導致 GSH 含量減少及 caspase-3 活化之現象。
綜合上述結果，女貞子具神經細胞保護之作用，主要活性分層為乙酸乙酯層，其作用機轉可能因其所含總酚類成分之抗氧化活性，減少細胞氧化壓力並降低GSH消耗，因而降低細胞凋亡訊息caspase-3活性以增加細胞之存活有關。

Neurodegenerative diseases, such as Alzheimer’s disease, are closely related to free radical overloading and intracellular oxidative stress. Therefore, it is suggested antioxidants may be useful for the prevention and treatment of neurodegenerative diseases. Ligustri Lucidi Fructus is the dry ripe fruits of Ligustrum lucidum Ait. (Oleaceae). In this study the antioxidant activities, and antioxidant constituents of Ligustri Lucidi Fructus, Ecliptae Herba, and Erzhi Wan, were evaluated. The neuroprotecitvie effects of Ligustri Lucidi Fructus on hydrogen peroxide induced oxidative damage in SH-SY5Y cells were examined.
The experimental results demonstrated that: 1, Erzhi Wan exhibits stronger antioxidant activity than Ligustri Lucidi Fructus and Ecliptae Herba. Crude extract of Ligustri Lucidi Fructus scavenges superoxide anion, hydroxyl and peroxyl radical, and inhibits lipid peroxidation. Among all subfractions, ethyl acetate fraction possesses highest activities in antioxidation and scavenging superoxide anion; however, chloroform fraction possesses highest activities in scavenging hydroxyl and peroxyl radical.
2, crude extract of Ligustri Lucidi Fructus elevates cell viability, decreases intracellular oxidative stress and reverses the decrease in GSH contents and GSH/GSSG ratio in hydrogen peroxide treated SH-SY5Y cells. Ethyl acetate fraction possesses better neuroprotective activity than chloroform fractions in hydrogen peroxide induced neuronal oxidative damage. Ethyl acetate fraction also decreases intracellular oxidative stress, and reverses the decrease in GSH contents and caspase-3 activation caused by hydrogen peroxide.
Thus, Ligustri Lucidi Fructus possesses neuroprotective effects and its active constituents may exist in ethyl acetate fraction. The neuroprotective effects of Ligustri Lucidi Fructus might be due to its phenolic antioxidant activities, and decreasing intracellular oxidative stress, GSH consumption and caspase-3 activation by hydrogen peroxide.

目 錄 I
略字表 III
圖目錄 IV
表目錄 VII
中文摘要 IX
英文摘要 XI
第一章 緒　論 1
第二章 總　論 3
第一節 女貞子之文獻考察 3
一、 女貞子之本草學考察 3
二、 女貞子之藥用植物學考察 9
三、 女貞子之生藥學考察 10
四、 女貞子之成分文獻考察 14
五、 女貞子藥理作用之文獻考察 16
第二節 自由基之文獻考察 19
一、 自由基之特性與種類 19
二、 抗氧化防禦系統 28
第三節 氧化損傷與神經退化性疾病 32
一、 氧化損傷 32
二、 氧化損傷與神經退化性疾病之關係 33
三、 H2O2誘導SH-SY5Y神經細胞氧化損傷之實驗模式 33
第三章 實驗材料與方法 35
第一節 實驗材料 35
一、 女貞子、墨旱蓮與二至丸水煎液製備 35
二、 女貞子之萃取流程 35
三、 實驗試劑 36
四、 細胞培養 36
五、 實驗儀器 36
第二節 實驗方法 37
一、 二至丸、女貞子、墨旱蓮之總抗氧化能力測定 37
二、 女貞子之抗氧化成分含量測定 37
三、 女貞子之抗氧化活性分析 39
四、 女貞子對H2O2誘導神經細胞氧化損傷之保護作用 42
第三節 統計分析 44
第四章 實驗結果 45
一、 二至丸的總抗氧化能力 45
二、 女貞子之抗氧化成分含量測定 45
三、 女貞子之抗氧化活性分析 49
四、 女貞子對H2O2誘導神經細胞氧化損傷之保護作用 66
第五章 討　論 73
參考文獻 81

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