跳到主要內容

臺灣博碩士論文加值系統

(100.28.2.72) 您好!臺灣時間:2024/06/16 06:44
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:吳俊志
研究生(外文):Jun-Zhi Wu
論文名稱:維他命C磷酸鎂鹽水膠面膜的製造與穿透性試驗
論文名稱(外文):The manufacture of the Magnesium ascorbyl phosphate hydrogel mask and penetration test
指導教授:許世興許世興引用關係
指導教授(外文):Ceshing Sheu
學位類別:碩士
校院名稱:朝陽科技大學
系所名稱:應用化學系碩士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:56
中文關鍵詞:高效能液相層析儀經皮穿透維他命C磷酸鎂鹽
外文關鍵詞:High-performance liquid chromatographytransdermal penetratesMagnesium Asocorbyl Phosphate
相關次數:
  • 被引用被引用:10
  • 點閱點閱:907
  • 評分評分:
  • 下載下載:10
  • 收藏至我的研究室書目清單書目收藏:1
本研究使用維他命C的安定性衍生物維他命C磷酸鎂鹽(Magnesium ascorbyl phosphate;MAP)為材料,研發製作具長效且安定的水膠面膜並添加月桂酸當促進劑予以改善配方。再利用高效能液相層析儀(HPLC),探討MAP水膠面膜製作及保存之最適條件,包含成分安定性、經皮穿透性等等。
經由高效能液相層析儀,發現酸鹼值對於MAP的安定性有明顯的影響,酸鹼值控制在中性時,成品將有較好的安定性,經過150天保存後含量仍有90.4%。經皮穿透實驗方面,在配方中添加月桂酸當促進劑的水膠面膜在對應大兔腹部皮膚的試驗,穿透率比未添加月桂酸的水膠面膜高30%以上。
HPLC的分析採用ODS Hypersil 250×4.6mm(5μ)的管柱,移動相以含0.5mM Tetrabutylammonium bromide(TBA-Br)離子對試劑(Ion-pair reagent)和50mM Na2HPO4•2H2O的緩衝液、移動相流速1.0ml/min、偵檢器檢出波長259nm,此分析方法可在8分鐘內完成。
In this searsch, the more aqueous stable material of L-ascobic acid derivative, being Magnesium Ascobyl Phosphate( MAP ) was formulate in the hydrogel mask for the stability study. Further more, the addition of Lauric acid in the formulation was pwnwtration enhances whether it world taciliate the MAP release through transdermal penetration. The High-performance liquid chromatography( HPLC ) was capitalized for the analytical work.

From the HPLC results, if was shown that MAP stability was dependent on pH of the formulation. As pH of 7, the hydrogel still residue 90% MAP after 150-day storage. In the transdermal penetration test, the formulation comprising lauric acid provided 30% more MAP release than those without lauric acid when the stomach skin of rabbit was used as a membrane for testing.

As for HPLC analysis, an ODS hypersil column (250 x 4.6 mm ; 5μm ) was used with a mobil phase consisting of 0.5mM Tetrabutylammonium bromide ( TBA-Br ) and 50 mM disodium hydrogen phosphate as a buffer. An UV detector set as 259nm wave length and the elution rate of 1.0mL/min were utilized. In general, each run about eight minutes of time.
目 錄

中文摘要…………………………………………………………………I
英文摘要………………………………………………………………II
誌謝……………………………………………………………………III
目錄……………………………………………………………………IV
圖目錄…………………………………………………………………VI
表目錄………………………………………………………………VIII

第一章 緒論……………………………………………………………1
一、 簡介………………………………………………………………1
二、 研究目的…………………………………………………………2
三、 前言………………………………………………………………3
四、 文獻回顧…………………………………………………………17
五、 相關成分介紹……………………………………………………19
第二章 實驗……………………………………………………………23
一、 藥品……………………………………………………………23
二、 儀器設備………………………………………………………24
三、 分析方法與條件之建立…………………………………………25
四、 面膜的製備………………………………………………………26
五、 空白水膠面膜的製備……………………………………………26
六、 標準品溶液的配製………………………………………………27
七、 不同酸鹼值對標準品溶液安定性的影響………………………27
八、 水膠面膜中成分的萃取…………………………………………27
九、 面膜中百分比含量與釋離量的計算……………………………28
十、 經皮穿透實驗……………………………………………………29
十一、分析方法的表現………………………………………………31
第三章 結果與討論……………………………………………………33
一、 不同酸鹼值對標準品溶液安定性的影響……………………33
二、 面膜成品在儲存期間的安定性………………………………35
三、 面膜成品的經皮穿透…………………………………………37
四、 時間對於水膠面膜外觀的影響………………………………45
五、 分析方法的表現………………………………………………46
1. 專一性(specificity)…………………………………46
2. 精密度(precision)……………………………………48
3. 準確度(accuracy)………………………………………50
4. 線性(linearty)…………………………………………50
5. 儀器偵測極限(LOD)……………………………………52
6. 分離因子導出結果…………………………………………52
第四章 結論……………………………………………………………53
第五章 參考文獻………………………………………………………54







圖 目 錄

圖1 表皮的三種不同的細胞 …………………………………………3
圖2 黑色素生成途徑……………………………………………………5
圖3 Hydroquinone、Arbutin及Kojic acid對黑色素形成抑制圖示…………………………………………………………………………7
圖4 維他命C的結構式…………………………………………………19
圖5 維他命C磷酸鎂鹽的結構式………………………………………21
圖6 月桂酸的結構式…………………………………………………22
圖7 維他命C磷酸鎂鹽之UV吸收圖.…………………………………25
圖8維他命C之UV吸收圖………………………………………………25
圖9直立式恆溫槽示意圖.……………………………………………29
圖10a直立式恆溫反應槽裝置圖………………………………………30
圖10b直立式恆溫反應槽的串聯………………………………………30
圖11維他命C磷酸鎂鹽標準溶液在各種酸鹼值環境下的安定性曲線34
圖12a面膜(未添加月桂酸)在儲存期間成份的含量變化…………36
圖12b面膜(添加月桂酸)在儲存期間成份的含量變化……………36
圖13a兩種水膠面膜在Nylon濾膜上所呈現的經皮穿透曲線圖……38
圖13b兩種水膠面膜在兔皮上所呈現的經皮穿透曲線圖……………40
圖14月桂酸的含量對於成分單位面積釋離量的變化………………42
圖15兩種維他命C磷酸鎂鹽水膠面膜在150天內的成分釋離量濃度變化………………………………………………………………………44
圖16維他命C磷酸鎂鹽標準品100ppm在HPLC的層析圖………………46
圖16b維他命C磷酸鎂鹽用Diode Array偵檢器之吸收圖……………46
圖17a維他命C磷酸鎂鹽水膠面膜樣品(未添加月桂酸)在HPLC的層析圖……………………………………………………………………47
圖17b維他命C磷酸鎂鹽水膠面膜樣品(添加月桂酸)在HPLC的層析圖………………………………………………………………………47
圖18維他命C磷酸鎂鹽標準品檢量線圖………………………………51









表目錄

表一、常見的外用藥物所使用的促進劑其作用原理與類型………10
表二、正相層析與逆相層析之特性比較……………………………13
表三、不同尺寸應用需求之管柱尺寸………………………………14
表四、各種型態層析管及其官能基…………………………………15
表五、高效能液相層析儀常用偵檢器之特性比較…………………16
表六、不同酸鹼值環境下,對於標準品溶液的濃度影響變化……33
表七、時間對於面膜中維他命C磷酸鎂鹽成分的含量變化數據……35
表八、兩種水膠面膜在Nylon濾膜的經皮穿透數據…………………37
表九、兩種水膠面膜在大兔腹部皮膚的經皮穿透數據……………39
表十、月桂酸的含量對於成分單位面積釋離量的數據……………41
表十一、兩種水膠面膜在150天內主成分釋離量數據………………43
表十二、兩種維他命C磷酸鎂鹽水膠面膜在儲存期間的外觀變化…45
表十三、維他命C磷酸鎂鹽標準溶液經HPLC分析之再現性數據……48
表十四、維他命C磷酸鎂鹽的回收率數據……………………………50
表十五、經由HPLC分析不同濃度維他命C磷酸鎂鹽標準品溶液的數據………………………………………………………………………51
1.Chang, L. C., Application of cosmetics Inspecting, Fayfar Publishing Co., Ltd, Taiwan, pp. 243-246 (1998).

2.Chen. W. D., Zheng, H. W., New Cosmetics, He Ji Publishing Co., Ltd, (2000).

3.Xu, L. R. “The Study on the Percutaneous Absorption of Piroxicam FAPG Ointment,” Thesis for the degree of Master, Kaohsiung Medical University,(2002).

4.Yang, C. R., “Optimization of Lipase-Catalyzed L-Ascorbyl Laurate by Response Surface Methodology,” Thesis for the degree of Master, Department of Bioindustry Technology, Da-Yeh University,(2001).

5.Li, Xin, & Wang, Hue,“Synthetical Evaluation of Promoting Effect of Some Kinds of Transdermal Enhancers with Grey Relational Cluster Method,” China Journal of Chinese Materia Medical, 1001-5302(2001)05-0417-04 (2001).

6.Chen. W. D., Zheng, H. W., New Cosmetics, He Ji Publishing Co., Ltd, (2000).

7.Zhou, J. H., “Introduction of the whitening cosmetics,” A news letter of food and drug, Vol. 256, pp.3-5 (2002).

8.Chang, H. C., “The safety and quality studies of Chinese herbs and their in vivo evaluations of whitening effect on human subjects,” Ms. Dissertation, Graduate Institute of Pharmaceutical chemistry, University of china medical, (2001).

9.Lowell A. Golgsmith, “Biochemistry and Physiology of the Skin 1983,” Oxford University Press,Inc.

10.Jara, J. R., Salano, F., Garcia-Borron, J. C., Aroca, P., and Lozano, J. A., “Regulation of mammalian melanogenesis. II: The role of metal cations, ”Biochimica. Et Biophysica. Acta, Vol. 1035, No. 3, pp.276-285 (1990).

11.Duckworth, H. W., Coleman, J. E., “Physicochemical and kinetic properties of mushroom tyrosinase,” J. Biol. Chem, Vol. 245, No. 7, pp. 1613-1625 (1970).

12.Linda, L. H., Pharm., D., “Use of hydroquinone as a bleaching cream,” Ann. Pharmacotherapy, No. 27, pp. 592-593 (1993).

13.Zhang, J. X., Quan, M. Y., Qiu, X. Q., Science on the skin operation, He Ji Publishing Co., Ltd, (1990).

14.Kazuhisa, M., Minoru, F., “In vitro effectiveness of several whitening cosmetic components in human melanocytes,” J. Soc. Cosmet. Chem, No. 42, pp. 361-368 (1991).

15.Schallreuter, K.U., Wood, J.W., “A possible mechanism of action for azelaic acid in the human epidermis,” Arch. Dermatol. Res, No. 282, pp. 168-171 (1990).

16.Chu, H. L., Shaw, J. F., “Biochemical characterization of isozymes of bighead shrimp tyrosinase,” J. Chinese. Agri. Chem. Soc., No. 27, pp. 360-372 (1989).

17.Chang, L. C., Application of cosmetics Inspecting, Fayfar Publishing Co., Ltd, Taiwan, pp. 243-246 (1998).


18.D. A. Skoog., F. J. Hooller., T. A.Nieman.,“Principle of Instrumental Analysis, Fifth edition, Harcourt Brance and Company Florida, pp.673~696,725~750 (1997).

19.R. K. Chen.,“Principle of LC-MS-MS and its Application in Food Analysis Food Industries,” Taiwan R.O.C, Vol.33, No.3, pp.44~54 (2001).

20.C. J. Argoudelis.,“Isocratic Liquid Chromatograph Method for Aspartame and Other Additives in Soft Drinks,” Journal of Chromatography, 303, pp.256~62 (1984).

21.H. D. Hua.,“The Main Points of Choose HPLC Column,” Agilent Technologies, Taiwan R.O.C, No.3, (2000).

22.Sandie Lindsay, “High Performance Liquid Chromatography,” 2nd edition, London: Wiley (1992).

23.W.H.A. Niessen and J. van der Greef, Liquid Chromatography, “Mass Spectrometry Principles and Applications”, 1st edition, New York: Dekker (1992).

24.Semenzato, A., Austria, C., Dall’Aglio, and Bettero A., “High-performance liquid chromatographic determination of ionic compounds in cosmetic emulsions: application to magnesium ascorbyl phosphate,” Journal of Chromatography A, No. 705, pp.385-389 (1995).

25.Austria, C., Semenzato, A., and Bettero A., “Stability of vitamin C derivatives in solution and topical formulations,” Journal of Pharmaceutical Biomedical Analysis, No.15, pp. 795-801 (1997).

26.Salai, T., Murata, H., and Ito, T., “High-performance liquid chromatographic analysis of ascorbyl-2-phosphate in fish tissues,” Journal of Chromatography B, No.685, pp. 196-198 (1996).

27.Sottofattori, E., Anzaldi, M., Balbi, A., and Tonello, G., “Simultaneous HPLC determination of multiple components in a commercial cosmetic cream, ” Journal of Pharmaceutical Biomedical Analysis, No.18, pp. 213-217 (1998).

28.Shih, Y., “Simultaneous determination of Magnesium L-Ascorbyl-2-Phosphate and Kojic acid in Cosmetic Bleaching Products by Using a Microbore Column and Ion-Pair Liquid Chromatography, ” Journal of AOAC INTERNATIONAL,Vol.84, No.4 (2001).
電子全文 電子全文(本篇電子全文限研究生所屬學校校內系統及IP範圍內開放)
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top