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研究生:鄭宗堯
研究生(外文):Tsung-Yao Cheng
論文名稱:真菌Aspergillusterreus生產lovastatin之增產策略
論文名稱(外文):Enhanced strategies for lovastatin production by Aspergillus terreus
指導教授:賴龍山賴龍山引用關係
指導教授(外文):Long-Shan Lai
學位類別:碩士
校院名稱:朝陽科技大學
系所名稱:應用化學系碩士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:89
中文關鍵詞:溫度轉換Aspergillus terreusLovastatin
外文關鍵詞:Aspergillus terreusLovastatinTemperature-shift
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本研究以Aspergillus terreus培養在28oC、5升醱酵槽,實驗結果說明在0-48小時的溶氧供應主宰此真菌菌絲顆粒之形成,也直接增加lovastatin的產量。配合溶氧(DO)控制在相對飽和度20%,本文發現在第4天將培養溫度由28oC降為23oC,在與恆溫(28oC)對照組比較,其lovastatin的最大產量可提升20%;此時,菌體產量卻只減少6%。使用此溫度轉換時,醱酵液中溶氧確會顯著增加因而導致細胞生理的變化(如pH變動趨勢、糖源利用等),可能因此造成產物的增產。但是,在使用5升醱酵槽若以轉速200 rpm控制時,儘管在醱酵後期會提升溶氧濃度,但降溫操作卻使產量稍低。基於本實驗結果,我們歸納出影響產物lovastatin生合成的主因是在細胞生長階段培養環境溶氧的供應充分與否。
Using a 5 l fermentor at 28oC, the dissolved O2 (DO) supplement of 0-48 h dominated pellet formation of Aspergillus terreus, which substantially increased the synthesis of lovastatin. Coupled with the DO comparatively controlled at 20%, a simple temperature-shift (28-23oC at day 4) was proved valuable, where the maximum of lovastatin production was enhanced by 20% in comparison with that at 28oC. Nevertheless, the product enhancement was only accompanied with a 6% reduction in biomass production. It was found that the temperature-shift method used here significantly increased the DO, closely altered the physiological states (such as the pH kinetics, sugar utilization etc.), probably thus resulting in the enhancement of product formation. However, using a 5 l fermentor operated at 200 rpm, this same temperature-shift contradictorily gave a slightly negative effect on product formation although the DO in broth was raised up in later stages of the fungal fermentation. According to the results of such experiments, we concluded that the disparity in product formation was mainly attributed to the difference of DO supplies in earlier growth stage of the fungus.
目 錄
中文摘要……………………………………………………Ⅰ
英文摘要……………………………………………………Ⅱ
表目錄………………………………………………………Ⅲ
圖目錄………………………………………………………Ⅳ


第一章 前言
1.1 研究動機………………………………………………1
1.2 研究目的………………………………………………4


第二章 文獻回顧
2.1膽固醇簡介………………………………………………6
2.2膽固醇抑制劑…………………………………………11
2.3真菌代謝物生產………………………………………19
2.3.1真菌的一次代謝物生產…………………………19
2.3.2真菌的二次代謝物生產…………………………22
2.4真菌醱酵技術…………………………………………30
第三章 材料與方法
3.1菌株……………………………………………………38
3.2實驗藥品及培養基
3.2.1實驗藥品…………………………………………39
3.2.2實驗培養基………………………………………41
3.3實驗儀器設備…………………………………………43
3.4實驗步驟
3.4.1搖瓶實驗…………………………………………45
3.4.2五升醱酵槽實驗…………………………………46
3.5分析方法
3.5.1細胞乾重…………………………………………48
3.5.2產物萃取…………………………………………48
3.5.3粒徑分佈…………………………………………48
3.5.4 lovastatin濃度定量分析………………………49
3.5.5殘糖濃度分析……………………………………49


第四章 結果與討論…………………………………50
4.1溶氧的重要性…………………………………………51
4.2變換溫度之影響………………………………………56
第五章 結論與建議…………………………………60
5.1結論……………………………………………………60
5.2建議……………………………………………………63


數據圖表…………………………………………………64


參考文獻…………………………………………………74



附錄
ㄧ、lovastatin濃度之校正曲線與HPLC之圖譜…………85
二、殘糖濃度之校正曲線…………………………………87
三、五升醱酵槽操作步驟…………………………………88
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