本實驗以番茄(Lycopersicon esculentum)做為研究的對象，探討植物和病原菌及植食昆蟲三者之間的交互作用。而所選用的植物病原菌和植食昆蟲分別為番茄嵌紋病毒(tomato mosaic virus, ToMV)與番茄夜蛾(Helicoverpa armigera)。實驗則主要偏重於探討番茄夜蛾的行為和生長表現(performance)，是否受到先前已感染病毒或是被蟲啃食的植物所影響，以及其影響的程度。在對植株進行病毒接種及蟲咬處理之後，分別藉由番茄夜蛾的取食偏好、產卵偏好、長期餵食分析以及短期餵食分析實驗，來探討各處理間的差異。結果顯示，接種病毒的植株會降低番茄夜蛾成蟲體重、四齡幼蟲消化食物轉換率(ECD)，以及消耗食物轉換率(ECI)，但在行為方面並無選擇偏好上的差異；而已先被昆蟲啃食過的植物則是會對於番茄夜蛾的取食和產卵行為具有忌避效果，並且也降低四齡幼蟲的消化效率(AD)、總消耗量(TC)、相對生長速率(RGR)、相對消耗速率(RCR)，以及延長發育天數(DUR)。此外，更進一步對被施予病毒接種處理及蟲咬處理的番茄在不同時間點進行化學成分分析，以證明病毒和昆蟲是否會改變及誘發植物體內的化學成分。結果顯示病毒與昆蟲之處理分別提高植物體內過氧化酵素(Peroxidase)和多酚氧化酵素(Polyphenol oxidase)的活性，且改變非結構性碳水化合物(nonstructural carbohydrates)的含量。佐以這些化學分析的證據可得知，夜蛾取食植株後能明顯影響植物體內化學成分改變以及後續取食該植物的番茄夜蛾生長表現。

The objective of this study is to investigate interactions between plants, phytopathogens, and herbivores. Tomato (Lycopersicon esculentum) was used as the test plant, and tomato mosaic virus (ToMV) and corn earworm (Helicoverpa armigera) were used as phytopathogen and herbivore, respectively. This study focused primarily on the performance of corn earworm which was reared on the tomato leaf that had been infected with ToMV or attacked by corn earworms. The performance parameters, such as feeding preference, oviposition preference, growth rates, consumption rates, and food processing efficiencies, were compared between treatments. The result shows that corn earworm had decreased adult weight, efficiency of conversion of ingested food (ECI), and efficiency of conversion of digest food (ECD) reared on tomato plants inoculated with ToMV. However, no difference was found in the feeding and oviposition preference trial. In contrast, tomato plants which damaged by corn earworm would deter the later corn earworms’ feeding and oviposition. Corn earworm’s approximate digestibility (AD), total consumption (TC), relative growth rate (RGR), and relative consumption rate (RCR) were decreased. In addition, the development duration (DUR) was prolonged in this assay. Furthermore, after attacked by ToMV and corn earworm, plant chemistry was analyzed at different time intervals. Results revealed that ToMV infection and corn earworm injury can increase host plant’s peroxidase and polyphenol oxidase activity, respectively. Besides, both ToMV and corn earworm would alter host plants’ soluble and non-soluble carbohydrate concentrations. Based on the chemical analysis results, we found that corn earworm’s performance may be affected by host plant chemistry. Overall, insect injury has stronger effect on plant chemistry and corn earworm’s performance than virus infection.

目錄
壹、中文摘要-----------------------------------------------1
貳、前言---------------------------------------------------2
參、前人研究-----------------------------------------------4
肆、材料與方法--------------------------------------------14
一、番茄栽培--------------------------------------------14
二、供試昆蟲飼養----------------------------------------14
三、植物病原--------------------------------------------14
四、試驗方式--------------------------------------------14
A.接種病毒--------------------------------------------15
B.蟲咬處理--------------------------------------------15
(一)、取食偏好試驗------------------------------------15
(二)、產卵偏好試驗------------------------------------16
(三)、長期餵食分析------------------------------------16
(四)、短期餵食分析------------------------------------17
(五)、植物化學成分分析--------------------------------19
(六)、統計分析----------------------------------------22
伍、結果--------------------------------------------------23
陸、討論--------------------------------------------------27
柒、參考文獻----------------------------------------------35
捌、英文摘要----------------------------------------------46
玖、圖表--------------------------------------------------48

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