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研究生(外文):Yuan-Chang Hsu
論文名稱(外文):Effects of Chronic Exercise and Acute Stress on Tissue HSP72 Expression in Rat
指導教授(外文):Chauying J. Jen
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  壓力反應雖為生物體因應周遭刺激之保護作用,但也可能即時引發心臟血管疾病。相對地,規律性運動可降低心臟血管疾病之發病率或減緩其嚴重性。文獻中指出透過壓力的刺激可以使血管造成heat shock protein 72 (HSP72) 的表現,近期亦有文獻指出給予大白鼠主動式滾輪運動 (habitual physical activity) 可使腦部、身體周邊及免疫組織的細胞在受壓力時較易產生HSP72來增強抗壓的耐受力。此外,HSP72在中樞調控血壓的相關腦區:孤獨核 (Nucleus tractus solitarii, NTS) 與延腦鼻端網狀腹外側核 (Rostral ventrolateral medulla, RVLM) 之表現,對調控心血管功能扮演重要角色。本研究方法分為兩個階段;第一階段為建立急性壓力模式,第二階段給予Wistar大白鼠兩種長期 (八週) 運動模式:a) 主動式滾輪運動,或b) 強制式跑步機運動。大白鼠於長期運動訓練後,以鐵絲網纏繞並浸於1.5 cm水中三十分鐘 (急性壓力模式),接著在休息三十分鐘後犧牲,取其NTS、RVLM、胸主動脈及腎上腺。以西方墨點法偵測其中HSP72蛋白表現。結果顯示一) 壓力使得大白鼠的血壓持續升高;二) 壓力使得胸主動脈及腎上腺表現HSP72蛋白;三) 長期主動式滾輪運動使得腎上腺及RVLM表現HSP72蛋白;四) 長期強制式跑步機運動則使胸主動脈及腎上腺表現HSP72蛋白;五) 壓力使長期強制式跑步機運動訓練鼠胸主動脈及腎上腺表現更多HSP72蛋白。因此我們認為兩種不同的模式以不同的機制調節大白鼠受壓時心血管的功能:亦即主動式滾輪運動增加中樞血壓調控中心RVLM與腎上腺之HSP72表現,而強制式跑步機運動則使血管及腎上腺之HSP72表現明顯上升。
 Although the acute stress response is important for the survival of animals, stress is a well-known cardiovascular disease risk factor. In contrast, regular exercise has been shown to have beneficial effects in the prevention of cardiovascular diseases. Long-term wheel running suppresses excessive increases in blood pressure and heart rate in rats under stress. How chronic exercise ameliorates the stress-associated adverse effects is an intriguing question. An early study has shown that restraint stress evokes HSP72 expression in the aorta. Recent results also indicate that habitual physical activity enhances stress-induced HSP72 induction in brain and various peripheral tissues, including adrenal gland, liver, and immune tissue. Moreover, elevated HSP72 expression in nucleus tractus solitarii (NTS) and rostral ventrolateral medulla (RVLM) improves the baroreflex capacity when rats are under heat stroke or sepsis. We, therefore, hypothesize that the beneficial effects of chronic exercise on cardiovascular function may be accompanied with elevated stress-induced HSP72 expression in various peripheral and central tissues. In this study, male Wistar rats underwent exercise training for 8 weeks either by exposure to a running wheel (voluntary exercise) or by running on a treadmill (compulsive exercise). At the end of exercise training period, they were subjected to restraint/water exposure stress before sacrifice by CO2 euthanasia. Western blot analysis was used to quantify the protein levels of HSP72 in adrenal gland, aorta, NTS and RVLM. Our results showed that 1) blood pressure was elevated and maintained at high level in rats subjected to restraint/water exposure stress, but only transiently elevated in rats under restraint stress alone; 2) both adrenal gland and aorta expressed HSP72 protein when rats were subjected to restraint/water exposure stress; 3) voluntary exercise training induced the HSP72 expression in adrenal gland and RVLM; 4) compulsive exercise training induced the HSP72 expression in adrenal gland and aorta; and 5) stress induced additional HSP72 elevation in adrenal gland and aorta of compulsive exercise trained rats. In conclusion, the HSP72 expression is differentially upregulated in various peripheral and central tissues by two different types of exercise training.
中文摘要--------------------------------------------------- 1
英文摘要--------------------------------------------------- 2
導論------------------------------------------------------- 3
實驗材料與方法--------------------------------- --------- 7
實驗結果-------------------------------------------------- 12
討論------------------------------------------------------ 17
表-------------------------------------------------------- 23
圖-------------------------------------------------------- 24
附錄------------------------------------------------------ 38
參考文獻-------------------------------------------------- 40
作者簡介-------------------------------------------------- 45
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