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研究生:古琇瑩
研究生(外文):Ku shiou ying
論文名稱:機動車輛廢氣暴露對氧化壓力及基因毒性影響之研究
論文名稱(外文):Exposure to traffic exhausts on oxidative stress and genotoxicity
指導教授:賴錦皇賴錦皇引用關係
學位類別:碩士
校院名稱:國防醫學院
系所名稱:公共衛生學研究所
學門:醫藥衛生學門
學類:公共衛生學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
中文關鍵詞:高速公路收費人員微小核細胞頻率基因毒性氧化傷害8-羥基去氧鳥糞嘌呤核苷
外文關鍵詞:highway toll station workersmicronucleigenotoxicityoxidative DNA damage8-hydroxy-2’-deoxyguanosine
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機動車輛廢氣是台灣空氣污染的主要來源,車輛排放的廢氣為複雜的混和物,包含了多環芳香烴、懸浮微粒等有毒物質,這些有毒物質除了可增加體內自由基的產生也會增加體內基因毒性。本研究目的在於利用橫斷性研究評估暴露於機動車輛廢氣收費員與基因毒性之關係,並探討收費員氧化性DNA傷害與基因毒性之相關;此外,利用縱斷性研究觀察收費員於五天工作期間尿中8-OHdG濃度變化。
本研究橫斷性研究選取47位女性高速公路收費員為暴露組,對照組則選取未暴露機動車輛廢氣之27位女性新訓人員,以微小核頻率為生物性指標進行分析;縱斷性研究則選取輪休後第一天上班31位女性收費員,進行5天追蹤上班前、下班後尿液8-OHdG分析,暴露評估則以累積車流量、累積PM2.5濃度及尿中1-OHPG濃度為指標,探討暴露指標與8-OHdG濃度變化之關係。
研究結果指出,暴露組微小核細胞頻率為1.00±1.34與對照組1.19±1.08無顯著差異,抽菸者微小核頻率1.83±1.59顯著高於非抽菸者0.94±1.06 (p<0.05),飲酒者微小核頻率2.33±0.58也顯著高於非飲酒者1.05±1.22 (p<0.05)。且以Spearman correlation分析尿中1-OHPG、8-OHdG濃度與微小核細胞頻率無顯著相關(r=-0.140, r=-0.035)。縱斷性研究結果顯示第二天下班後8-OHdG濃度為最高,第三天後8-OHdG濃度立即下降至與第一天濃度相似,但五天均是下班後8-OHdG濃度高於上班前。尿中8-OHdG濃度與累積車流量、PM2.5濃度及尿中1-OHPG濃度無顯著相關,此外,白班與大卡車尿中8-OHdG濃度與每日增加濃度都顯著為高。由本研究顯示暴露機動車輛廢氣會增加氧化性DNA傷害,但未增加基因毒性,且暴露機動車輛廢氣能短時間反映DNA傷害,此外人體也可能因抗氧化系統而使氧化性DNA傷害不再持續累積。
Motor vehicles are the major source of ambient air pollution in Taiwan. Traffic exhausts constitute a complex mixture and carcinogenic agents, such as polycyclic aromatic hydrocarbons (PAHs) and particulate matter (PM).The carcinogenic agents not only induce the formation of multiple free radical species but also increase the genotoxicity in human body. The first aim of this study was to evaluate the effects of traffic exhausts exposure and genotoxicity, and the relations between oxidative DNA damage and genotoxicity among highway toll station workers. The second aim aim was to determine the mean trajectories urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG).
Cross-sectional study of 49 female highway toll station workers exposed to traffic exhausts and 27 female office workers as a reference group. Micronuclei level was used to evaluate the genotoxicity of the traffic exhausts. Repeated-measurment cohort study compased of 31 female highway toll station workers. Spot urine samples were collected from each subject pre-shift and post-shift for 5 days of work after a day off. Exposure assessment was based on cumulative traffic density, cumulative concentration of PM2.5 and urinary 1-hydroxypyrene-glucuronide (1-OHPG).
The results showed no difference between exposure workers (1.00±1.34) and controls (1.19±1.08) for micronuclei, and smokers were significantly higher than nonsmokers (p<0.05), drinkers were also significantly higher than non-drinkers (p<0.05). Spearman correlation analysis the urinary concentration of 1-OHPG, 8-OHdG were no significantly correlated with micronucleus frequency (r=-0.14, r=-0.04). Repeated-measures study showed that the 2 day post-shift had the highest 8-OHdG level, then the 3 day decline to similar the start of the start of the workweek. In addition, tollworkers in pos-shift had higher 8-OHdG level than pre-shift for 5 days of work. Multivariate GEE analysis indicated that urinary 8-OHdG had no significantly correlated with cumulative traffic density, cumulative concentration of PM2.5 and urinary 1-OHPG. Besides, tollworkers worked in day-shift and truck tollbwth had the highest urinary 8-OHdG level. Although this study suggest that exposure to traffic exhausts increases oxidative DNA damage, neither increase genotoxicity. And antioxidant system induce in human body oxidative DNA damage didn’t consecutive cumulative.
正文目錄
正文目錄 I
附 錄 III
表目錄 IV
圖目錄 VI
中文摘要 VII
英文摘要 1
第一章 前 言 1
第一節 研究背景 1
第二節 研究目的 4
第二章 文獻探討 8
第一節 空氣污染之流行病學研究 8
第二節 微粒特性與成分相關研究 12
第三節 懸浮微粒與氧化壓力介紹 15
第四節 氧化壓力指標-8-hydroxydeoxyguanosine 19
第五節 基因毒性指標微小核(micronucleus, MN) 24
第三章 研究假設 28
第四章 材料與方法 29
第一節 研究對象與地理位置 29
第二節 研究工具與研究步驟 31
第三節 實驗品質管制 39
第四節 統計分析方法 41
第五章 研究結果 45
第一節 橫斷性研究對象描述性檢定 45
第二節 淋巴球微小核細胞頻率與微小核總數描述與檢定 47
第三節 縱斷性研究對象描述性檢定 52
第四節 縱斷性研究尿中8-OHdH變化 53
第六章 討 論 59
第一節 微小核細胞頻率與總微小核數 59
第二節 縱斷性研究尿中8-OHdG濃度變化探討 65
第七章 結論與建議 71
參 考 文 獻 73
參 考 文 獻
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