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研究生:陳昭寰
研究生(外文):Jau-Huan Chen
論文名稱:分子選殖吳郭魚啟動素βA次單元體基因及其應用於斑馬魚不孕性之研究
論文名稱(外文):Molecular cloning of activin βA subunit gene of tilapia (Oreochromis mossambicus) and its application on the sterilization of zebrafish (Danio rerio)
指導教授:陸振岡
指導教授(外文):Jenn-Kan Lu
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:102
中文關鍵詞:啟動素生殖不孕3D結構分子對位
外文關鍵詞:activinreproductionsterilization3D sturcturemolecular docking
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啟動素(activin)為一調控生殖生理賀爾蒙,被歸類為轉化生長因子β(TGF-β)超級家族一員。啟動素可刺激促濾泡激素的分泌與合成,亦能促進卵巢產生性類固醇賀爾蒙及促性腺釋放激素的合成與釋放。在本研究中,選殖出莫三鼻克吳郭魚啟動素βA次單位基因cDNA全長,包括1959個核苷酸,其中可演譯出458個胺基酸序列,一個238個核苷酸的5’端未轉譯區(5’ untranslation region, 5’ UTR)及一個344個核苷酸的3’端未轉譯區。根據Genomic DNA選殖結果顯示吳郭魚啟動素βA基因含有兩個外顯子(exon)及一個超過1.1 kb的內含子(intron)。將吳郭魚啟動素βA次單位基因與其它物種,如斑馬魚、金魚、蠑螈、雞、人類、小鼠、大鼠和豬等之啟動素βA次單位基因比較核酸同一性(identities)為48∼68%。吳郭魚啟動素βA次單位基因轉譯胺基酸序列及成熟胜肽序列與其它脊椎動物比較其相似性(similarities)分別為47∼65%及73∼91%。利用半定量RT-PCR分析吳郭魚各組織啟動素βA基因之表現模式,結果顯示吳郭魚生殖腺、腦及胃有較高表現量。利用比較分子模擬(comparative modeling)技術及分子對位(molecular docking)技術發現吳郭魚啟動素在finger 1功能區塊(domain)與啟動素第二型受體對位有較強之鍵結力。本研究中利用電腦虛擬篩選(in silico virtual screening)技術分別篩選出最適合與吳郭魚啟動素或魚類啟動素第二型受體接合位置之結合化合物(TRIPOS_1511_11501 _001與35085),此二分子可能影響啟動素之正常功能。利用啟動素第一型受體抑制劑SB-431542餵食斑馬魚,顯示可抑制斑馬魚卵巢發育及促進體細胞重。
Activin is a reproductive hormone and belongs to transforming growth factor β superfamily (TGF-β). It has been shown activin can simulate FSH and GnRH secretion, and gonadal steroidogenesis in mammals. In present study, a 1959 nucleotides of activin βA cDNA by RT-PCR method, encoded 458 amino acids was isolated from tilapia (Oreochromis mossambicus), containing a 238 bp 5’ untranslation region (UTR) fragment and a 344 bp 3’ UTR fragment. Activin βA genomic DNA cloning results indicated that activin βA gene containing two exons and an intron greater than 1.1kb. Comparing of tilapia activin βA gene with other vertebrates such as Denio ririo, Carassius aruatus, Cynops pyrrhogaster, Gallus gullas, Homo sapiens, Mus musculus, Rana nigromaculata, and Sus scrofa, the identities of nucleic acid sequence are ranging from 48% to 68%, respectively. Comparing of tilapia activin βA full length peptide and mature peptide sequence with other vertebrates the similarities of amino acid sequence are ranging 47~65% and 73~91%, respectively. Higher level expression of activin βA gene was detected in testis, ovary, brain, and stomach tissues. A strong binding site found in the finger 1 domain of tilapia Activin which can interact with activin type II receptor by molecular modeling and molecular docking techniques. Two most fit candidate molecules (TRIPOS_1511_11501_001 and 35085) can interact with tilapia activin and activin type II receptor, respectively, by molecular docking and in silico virtual screening techniques. An activin type I receptor inhibitor, SB-431542, was shown an inhibition activity on gonadal development and increasing somatic weight in zebrafish.
頁次
謝辭 i
中文摘要 ii
英文摘要 iii
目錄 iv
表目錄 vi
圖目錄 vii
壹、前言 1
一、基因轉殖水產生物之環境影響與不孕技術的研發 1
二、動物生殖生理與啟動素(Activin)調控之機制 2
三、啓動素、抑制素與卵泡抑素調控生殖生理功能及其訊息傳遞 3
四、啟動素受體(Activin receptor)6
五、轉化生長因子β超級家族(transforming growth factor β superfamily,TGF-β superfamily)7
六、分子模擬型化(Molecular modeling)、分子對位(Molecular docking)與化學資訊學(Chemoinformatics) 8
七、啟動素與啟動素第二型受體的結構模型(Structure modeling)與分子對位(Molecular docking) 9
八、哺乳動物剔除(knockout)啟動素相關基因在生殖生理及不孕性之影響 10
貮、材料與方法 14
一、實驗生物 14
二、實驗物種之蓄養 14
三、分子選殖(molecular cloning)莫三鼻克吳郭魚之Activin βA基因 15
四、吳郭魚啟動素βA 基因體DNA(Genomic DNA)序列選殖 23
五、莫三鼻克吳郭魚Activin βA次單元基因之特性分析 26
六、電腦模擬啟動素和啟動素第二型受體B三維立體結構與分子對位 30
七、餵食斑馬魚Activin type Ⅰ receptor抑制劑(SB-431542) 31
八、建立吳郭魚重組蛋白質(Recombinant Activin βA, rACTβA)之表現選殖 33
參、結果 38
肆、討論 45
伍、參考文獻 51
陸、圖表 61
附錄 89
伍、參考文獻
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