跳到主要內容

臺灣博碩士論文加值系統

(18.97.9.172) 您好!臺灣時間:2025/01/20 18:02
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:林子奕
研究生(外文):Tz -I Lin
論文名稱:探討血紅素氧化酶在樹突狀細胞的功能及CDK抑制劑抑制LPS誘導的樹突狀細胞成熟化
論文名稱(外文):Investigation of function of heme oxygenase in dendritic cell and CDK inhibitor, olomoucine, reduce LPS – mediated maturation of dendritic cell
指導教授:唐世杰
指導教授(外文):Shye-Jye Tang
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:73
中文關鍵詞:樹突狀細胞CDK抑制劑
外文關鍵詞:dendritic cellCDKI
相關次數:
  • 被引用被引用:0
  • 點閱點閱:252
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
樹突狀細胞是已知的免疫細胞中,最強的抗原呈獻細胞,在調節適應性免疫系統(adaptive immune system)上扮演著重要的角色。在臨床的應用上,可藉其可呈獻抗原的特性,來增強免疫系統抗腫瘤的能力。於是,如何促進樹突狀細胞活化、增加其抗原呈獻的能力,亦是一項重要的課題。另外已有文獻指出,參與heme代謝的heme oxygenase ( HO ),會促進T細胞發生因活化誘導引發的細胞死亡(activation-induced cell death, AICD),而影響生物體的免疫反應。因此,討論HO在免疫細胞內所扮演的角色亦是目前注目的研究方向。本研究中,便是利用HO抑制劑當作工具,以骨髓細胞經體外培養之樹突狀細胞為對象,將樹突狀細胞以血紅素氧化酶抑制劑進行前處理後,再探討血紅素氧化酶對於樹突狀細胞的影響,而進一步分析它在免疫治療中的角色。實驗結果顯示,抑制血紅素氧化酶能增加成熟中樹突狀細胞的存活率,此發現或許能提供新的策略來活化樹突狀細胞,進而增加抗腫瘤的專一性免疫。
使用LPS來誘導樹突狀細胞成熟化已是常見的實驗方法,CDK抑制劑在瞭解細胞週期的應用上也行之有年,然而以細胞生長停滯研究樹突狀細胞的成熟化卻是屈指可數。經實驗,抑制CDK活性使成熟中樹突狀細胞生長停滯會導致細胞死亡,並且降低樹突狀細胞經LPS刺激後產生NO的能力,減少由LPS刺激之樹突狀細胞CD11c、CD40的表現量。我們的研究結果有助於強化以LPS促細胞成熟,來設計樹突狀疫苗的效果;針對樹突狀細胞參與的自體免疫調控,也提供了一個新的研究方向。
Dendritic cells ( DC ) are potent antigen-presenting cells ( APC ) that play a key role in regulation of adaptive immune system. The cells are capable of presenting antigen to promote immune response. Heme oxygenase involves the metabolism of heme. This enzyme has shown that it modulates the allo-immune response by promoting activation-induced cell death of T cells. In this study, DC treated with HO inhibitor were observed the change on mix-lymphocyte reaction. In addition, the survive rate of dendritic cell was increased by the treatment of HO inhibitor during maturation.
DC was induced the maturation by using LPS. In previous study, olomoucine, cyclin-dependent kinase ( CDK ) inhibitor, may inhibit the pro-inflammatory response by reducing the activation of JNK and NF-κB. In this study, olomoucine might cause cell death during maturation, reduce the NO production, and decrease the expression of CD11c as well as CD40 after LPS stimulation. Our results provides a novel finding that olomoucine may affect LPS-mediated maturation of dendritic cell.
中文摘要……………………………………………………………5
英文摘要……………………………………………………………6
壹、序論………………………………………………….……….7
貳、實驗材料………………………………………………………18
參、實驗方法………………………………………………………20
肆、結 果………………………………………………………...27
伍、討 論………………………………………………………….33
陸、參考文獻………………………………………………………38
柒、圖表……………………………………………………………57
Abraham RT, Acquarone M, Andersen A, Asensi A, Belle R, Berger F, Bergounioux C, Brunn G, Buquet-Fagot C and Fagot D. 1995. Cellular effects of olomoucine, an inhibitor of cyclin-dependent kinases. Biol. Cell 83: 105-120

Aderem A, Ulevitch RJ. 2000. Toll-like receptors in the induction of the innate immune response. Nature 406: 782-7

Aiello S, Noris M, Piccinini G, Tomasoni S, Casiraghi F, Bonazzola S, Mister M, Sayegh MH, Remuzzi G. 2000. Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens. J. Immunol. 164: 4649–58

Akira S, Takeda K. 2004. TOLL-LIKE RECEPTOR SIGNALLING. Nat. Rev. Immunol. 4: 499-511

Amersi F, Buelow R, Kato H, Ke B, Coito AJ, Shen XD, Zhao D, Zaky J, Melinek J, Lassman CR. 1999. Upregulation of heme oxygenase-1 protects genetically fat Zucker rat livers from ischemia/reperfusion injury. J. Clin. Invest. 104:1631–1639

Andrews, D.M. et al. 2003. Functional interactions between dendritic
cells and NK cells during viral infection. Nat. Immunol. 4, 175–181

Banchereau J, Steinman RM. 1998. Dendritic cells and the control of immunity. Nature 392: 245-52

Banchereau J, Briere F, Caux C, Davoust J, Lebecque S, Liu Y-J, Pulendran B, Palucka K. 2000. IMMUNOBIOLOGY OF DENDRITIC CELLS. Annu. Rev. Immunol. 18: 767–811

Banchereau J, Palucka AK. 2005. DENDRITIC CELLS AS THERAPEUTIC VACCINES AGAINST CANCER. Nat. Rev. Immunol. 5: 296-306

Baranano DE, Snyder SH. 2001. Neural roles for heme oxygenase: contrasts to nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. 98: 10996–11002

Bhardwaj N, M.D., Ph.D. 2003. The modulation of immunity by dendritic cells. Clinical and Applied Immunology Reviews 3: 173-82

Bohnenkamp HR, Burchell JM, Taylor-Papadimitrioub J, Nolla T. 2004. Apoptosis of monocytes and the influence on yield of monocyte
-derived dendritic cells. J. Immunol. Methods 294: 67-80

Bredt D S, Hwang P M, Glatt, C E, Lowenstein C, Reed R R & Snyder S H. 1991. Cloned and expressed nitric oxide synthase structurally
resembles cytochrome-P450 reductase. Nature 351: 714–
718

Brouard S, Otterbein LE, Anrather J, Tobiasch E, Bach FH, Choi AM, and Soares MP. 2000. Carbon monoxide generated by heme oxygenase 1 suppresses endothelial cell apoptosis. J. Exp. Med. 192:1015–1026

Brouard S, Berberat PO, Tobiasch E, Seldon MP, Bach FH, and Soares MP. 2002. Heme oxygenase-1 derived carbon monoxide requires the activation of the transcription factor NF-kB to protect endothelial cells from TNF-alpha mediated apoptosis. J. Biol. Chem. 277: 17950–17961

Camara NOS, Soares MP. 2005. Heme oxygenase-1 (HO-1), a protective gene that prevents chronic graft dysfunction. Free Radic. Biol. Med. 38: 426-35

Caux C, Vanbervliet B, Massacrier C, Dezutter-Dambuyant C, Saint-Vis
B, Jacquet C, Yoneda K, Imamura S, Schmitt D, Banchereau J. 1996. CD34+ hematopoietic progenitors from human cord blood
differentiate along two independent dendritic cell pathways in response to GM-CSF+TNF alpha. J. Exp. Med. 184: 695-706

Chan FK, Zhang J, Cheng L, Shapiro DN and Winoto A. 1995. Identification of human/mouse p19, a novel CDK4/CDK6 inhibitor with homology to p16ink4. Mol. Cell. Biol. 15:2682–2688

Chang L, Karin M. 2001. Mammalian MAP kinase signalling cascades. Nature 410: 37-40

Chauveau C, Remy S, Royer PJ, Hill M, Tanguy-Royer S, Hubert F-X, Tesson L, Brion R, Beriou G, Gregoire M, Josien R, Cuturi MC, Anegon I. 2005. Heme oxygenase-1 expression inhibits dendritic cell maturation and pro-inflammatory function but conserves IL-10 expression. Blood DOI 10.1182/blood-2005-02-0494: 1-37

Chen J, Saha P, Kornbluth S, Dynlacht BD and Dutta A. 1996. Cyclin- binding motifs are essential for the function of p21Cip1. Mol. Cell. Biol. 16: 4673–4682

Clark JE, Foresti R, Green CJ, Motterlini R. 2000. Dynamics of haem oxygenase-1 expression and bilirubin production in cellular protection against oxidative stress. Biochem. J. 348: 615–619

Colonna M, Krug A, Cella M. 2002. Interferon-producing cells: on the front line in immune responses against pathogens. Curr. Opin. Immunol. 14: 373–9

Creusot RJ, Mitchison NA. 2004. How DCs control cross-regulation
between lymphocytes. Trends Immunol. 25: 126-31

Dawson T M, Snyder SH, 1994. Gases as biological messengers: nitric
oxide and carbon monoxide in the brain. J. Neurosci. 14, 5147–
5159

Fehsel, K., K. D. Kroncke, K. L. Meyer, H. Huber, V. Wahn, and
V. Kolb-Bachofen. 1995. Nitric oxide induces apoptosis in mouse thymocytes. J. Immunol. 155: 2858.

FERNANDEZ NC, LOZIER A, FLAMENT C, RICCIARDI
-CASTAGNOLI P, BELLET D, SUTER M, PERRICAUDET M, TURSZ T, MARASKOVSKY E, ZITVOGEL L. 1999. Dendritic cells directly trigger NK cell functions: Cross-talk relevant in innate anti-tumor immune responses in vivo. Nat. Med. 5: 405-11

Fujii S-i, Liu K, Smith C, Bonito AJ, Steinman RM. 2004. The Linkage of Innate to Adaptive Immunity via Maturing Dendritic Cells In Vivo Requires CD40 Ligation in Addition to Antigen Presentation and CD80/86 Costimulation. J. Exp. Med. 199: 1607-18

Galea E, Feinstein D L and Reis D J. 1992. Induction of calcium
-independent nitric oxide synthase activity in primary rat glial
cultures. Proc. Natl. Acad. Sci. USA 89: 10945-10949

Gerosa F, Baldani-Guerra B, Nisii C, Marchesini V, Carra G, Trinchieri G. 2002. Reciprocal Activating Interaction between Natural Killer
Cells and Dendritic Cells. J. Exp. Med. 195: 327-33

Glab N, Labidi B, Qin LX, Trehin C, Bergounioux C and Meijer L. 1994. Olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the G1 to S and G2 to M cell cycle transitions. FEBS Lett. 2:207-211

GRISHAM MB, JOURD’HEUIL D, WINK DA. 1999. Nitric Oxide
I. Physiological chemistry of nitric oxide and its metabolites: implications in inflammation. Am J Physiol. 276: G315-G21

Gu Y, Turck CW and Morgan DO. 1993. Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit. Nature 366:707–710

Hannon GJ and Beach D. 1994. p15INK4b is a potential effector of TGFβ-induced cell cycle arrest. Nature 371:257–261

Hilkens CM, Kalinski P, de Boer M, Kapsenberg ML. 1997. Human dendritic cells require exogenous interleukin-12-inducing factors to direct the development of naive T-helper cells toward the Th1 phenotype. Blood 90: 1920-1926

Hirai H, Roussel MF, Kato JY, Ashmun RA and Sherr CJ. 1995. Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol. Cell. Biol. 15: 2672–2681

Inguaggiato P, Gonzalez-Michaca L, Croatt AJ, Haggard JJ, Alam J, Nath KA. 2001. Cellular overexpression of heme oxygenase-1 up-regulates p21 and confers resistance to apoptosis. Kidney Int. 60: 2181–2191

Kampgen E, Koch F, Heufler C, Eggert A, Gill LL, Gillis S, Dower SK, Romani N, Schuler G. 1994. Understanding the dendritic ell lineage through a study of cytokine receptors. J. Exp. Med. 179: 1767-1776

Ke B, Shen XD, Zhai Y, Gao F, Busuttil RW, Volk HD, Kupiec-Weglinski JW. 2002. Heme oxygenase 1 mediates the immunomodulatory and antiapoptotic effects of interleukin 13 gene therapy in vivo and in vitro. Hum. Gene Ther. 13: 1845–1857

Kelleher M, Beverley PCL. 2001. Lipopolysaccharide modulation of dendritic cells is insufficient to mature dendritic cells to generate CTLs from naive polyclonal CD8+T cells in vitro, whereas CD40
ligation is essential. J. Immunol. 167: 6247–55

Kriehuber E, Bauer W, Charbonnier A-S, Winter D, Amatschek S, Tamandl D, Schweifer N, Stingl G, Maurer D. 2005. The balance between NF-kB and JNK/AP-1 activity controls dendritic cell life and death. Blood DOI 10.1182/blood-2004-08-3072: 1-43

Labeur MS, Roters B, Pers B, Mehling A, Luger TA, Schwarz T, Grabbe S. 1999. Generation of Tumor Immunity by Bone Marrow-Derived
Dendritic Cells Correlates with Dendritic Cell Maturation Stage. J. Immunol. 162: 168–75

Landmann S, Muhlethaler-Mottet A, Bernasconi L, Suter T, Waldburger J-M, Masternak K, Arrighi J-F, Hauser C, Fontana A, Reith W. 2001. Maturation of Dendritic Cells Is Accompanied by Rapid Transcriptional Silencing of Class II Transactivator (CIITA) Expression. J. Exp. Med. 194: 379-91

Lee MH, Reynisdottir I and Massague J. 1995. Cloning of p57Kip2, a
cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 9: 639–649

Lee TS, Chau LY. 2002. Heme oxygenase-1 mediates the anti-inflammatory effect of interleukin-10 in mice. Nat. Med. 8: 240-246

Liu N, Wang X, McCoubrey WK, and Maines MD. 2000. Developmentally regulated expression of two transcripts for heme oxygenase-2 with a first exon unique to rat testis: control by corticosterone of the oxygenase protein expression. Gene 241: 175–183

Luft T, Jefford M, Luetjens P, Toy T, Hochrein H, Masterman K-A, Maliszewski C, Shortman K, Cebon J, Maraskovsky E. 2002. Functionally distinct dendritic cell (DC) populations induced by physiologic stimuli: prostaglandin E2 regulates the migratory capacity of specific DC subsets. Blood 100: 1362-72

Lutz MB, Kukutsch N, Ogilivie A. LJ, Roβner S, Koch F, Romani N, Schuler G. 1999. An advanced culture method for generating quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods 223: 77-92

Maines MD. 1997. The heme oxygenase system: a regulator of second messenger gases. Annu. Rev. Pharmacol. Toxicol. 37:517–554

Maldonado-Lopez R, Smedt TD, Michel P, Godfroid J, Pajak B, Heirman C, Thielemans K, Leo O, Urbain J, Moser aM. 1999. CD8 alpha+ and CD8 alpha – subclasses of dendritic cells direct the development of distinct T helper cells in vivo. J. Exp. Med. 189: 587-92

Maraskovsky E, Brasel K, Teepe M, Roux E.R, Lyman SD, Shortman K, McKenna HJ. 1996. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic subpopulations identified. J. Exp. Med. 184: 1953-1962

Marletta MA. 1989. Nitric oxide: biosynthesis and biological significance. Trends Biochem Sci. 12: 488-92

Marletta MA. 1994. Nitric oxide synthase: aspects concerning structure and catalysis. Cell 78: 927–930

Matsue H, Edelbaum D, Shalhevet D, Mizumoto N, Yang C, Mummert ME, Oeda J, Masayasu H, Takashima A. 2003. Generation and function of reactive oxygen species in dendritic cells during antigen presentation. J. Immunol. 171: 3010-3018

Matsunaga T, Ishida T, Takekawa M, Nishimura S, Adachi M, Imai K. 2002. Analysis of gene expression during maturation of immature dendritic cells derived from peripheral blood monocytes. Scand J. Immunol. 56: 593-601.

Mattei F, Schiavoni G, Belardelli F, Tough DF. 2001. IL-15 Is Expressed by Dendritic Cells in Response to Type I IFN, Double-Stranded RNA, or Lipopolysaccharide and Promotes Dendritic Cell Activation. J. Immunol. 167: 1179–1187

McConnell BB, Gregory FJ, Stott FJ, Hara E and Peters G. 1999. Induced expression of p16INK4a inhibits both CDK4 and CDK2-associated kinase activity by reassortment of cyclin-CDK-inhibitor complexes. Mol. Cell. Biol. 19:1981–1989

McDaid J, Yamashita K, Chora A, Öllinger R, Strom TB, Li XC, Bach FH, Soares MP. 2005. Heme oxygenase-1 modulates the allo-immune response by promoting activation-induced cell death of T cells. FASEB J. doi:10.1096/fj.04-2217fje

McRae BL, Semnani RT, Hayes MP, van Seventer GA. 1998. Type I IFNs inhibit human dendritic cell IL-12 production and Th1 cell
development. J. Immunol. 160: 4298-4304

Melo LG, Agrawal R, Zhang L, Rezvani M, Mangi AA, Ehsan A, Griese DP, Dell’Acqua G, Mann MJ, Oyama J. 2002. Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase gene. Circulation 105: 602–607

Michelle Kelleher, Peter C. L. Beverley. 2001. Lipopolysaccharide Modulation of Dendritic Cells Is Insufficient to Mature Dendritic Cells to Generate CTLs from Naive Polyclonal CD8+ T Cells In Vitro, Whereas CD40 Ligation Is Essential. J. Immunol. 167: 6247–6255

Moncada S, Higgs EA. 1991. Endogenous nitric oxide: physiology, pathology and clinical relevance. Eur J Clin Invest. 4: 361-374

Montellano PR. 2000. The mechanism of heme oxygenase. Curr Opin Chem Biol 4:221–227.

Morse D, Choi AMK. 2002. Heme Oxygenase-1 The “Emerging Molecule” Has Arrived. Am. J. Respir. Cell Mol. Biol. 27: 8–16

Munder M., K. Eichmann, M. Modolell. 1998. Alternative metabolic states in murine macrophages reflected by the nitric oxide synthase/arginase balance: competitive regulation by CD41 T cells correlates with Th1/Th2 phenotype. J. Immunol. 160: 5347.

Munder M, Eichmann K, Mora´n JM, Centeno F, Soler Gn, Modolell M. 1999. Th1/Th2-Regulated Expression of Arginase Isoforms in Murine Macrophages and Dendritic Cells. J. Immunol. 163: 3771–7

Nathan CF, Hibbs JB Jr. 1991. Role of nitric oxide synthesis in macrophage anti-microbial activity. Curr. Opin. Immunol. 1: 65-70

Nathan C. 1992. Nitric oxide as a secretory product of mammalian cells. FASEB J. 12: 3051-64

Nathan C, Xie QW. 1994. Nitric oxide synthases: roles, tolls, and controls. Cell 78: 915–918

Nestle FO, Farkas A, Conrad C. 2005. Dendritic-cell-based therapeutic vaccination against cancer. Curr. Opin. Immunol. 17: 163–9

Nguyen, V. T., E. N. Benveniste. 2002. Critical role of TNF- and NF-B in IFN--induced CD40 expression in microglia/macrophages. J. Biol. Chem. 277: 13796-13803

Otterbein LE. Choi AMK. Heme oxygenase: colors of defense against cellular stress. Am. J. Physiol. 2000; 279: L1029–L1037

Otterbein LE. Soares MP. Yamashita K. Bach FH. 2003. Heme oxygenase-1: unleashing the protective properties of heme. Trends Immunol. 24: 449.

Otterbein LE. Bach FH. Alam J. Soares M. Tao Lu H. Wysk M. Davis RJ. Flavell RA. Choi AM. 2000. Carbon monoxide has anti-inflammatory effects involving the mitogenactivated protein kinase pathway. Nat Med. 6: 422-428

Pae H-O, Oh G-S, Choi B-M, Chae S-C, Kim Y-M, Chung K-R, Chung H-T. 2004. Carbon Monoxide Produced by Heme Oxygenase-1 Suppresses T cell Proliferation via Inhibition of IL-2 Production. J. Immunol. 172: 4744–51

Parry D. Bates S. Mann DJ. Peters G. 1995. Lack of cyclin D-cdk complexes in Rb-negative cells correlates with high levels of p16INK4/MTS1 tumor suppressor gene product. EMBO J. 14: 503–511

Parry D, Mahony D, Wills K and Lees E. 1999. Cyclin D-CDK subunit arrangement is dependent on the availability of competing INK4 and p21 class inhibitors. Mol. Cell. Biol. 19: 1775–1783

Piccioli D. Sbrana S. Melandri E. Valiante NM. 2002. Contact-dependent stimulation and inhibition of dendritic cells by natural killer cells. J. Exp. Med. 195: 335–341

Poss KD. Tonegawa S. 1997. Reduced stress defense in heme oxygenase
1-deficient cells. Proc. Natl. Acad. Sci. USA 94: 10925

PULENDRAN B, SMITH JL, G.CASPARY, BRASEL K, PETTIT D, E.MARASKOVSKY, MALISZEWSKI CR. 1999. Distinct dendritic cell subsets differentially regulate the class of immune response in vivo. Proc. Natl. Acad. Sci. USA 96: 1036-41

Rissoan MC, Soumelis V, Kadowaki N, Grouard G, Briere F, Malefyt RdW, Liu Y-J. 1999. Reciprocal control of T helper cell and dendritic cell differentiation. Science 283: 1183-6

Romani N. Gruner S. Brang D. Kampgen E. Lenz A. Trockenbacher B. Konwalinka G. Fritsch PO. Steinman RM. Schuler G. 1994 . Proliferating dendritic cell progenitors in human blood. J. Exp. Med. 180: 83-93

Russo AA. Jeffrey PD. Patten AK. Massague J. Pavletich NP. 1996. Crystal structure of the p27Kip1 cyclin-dependent kinase inhibitor bound to the cyclin A-cdk2 complex. Nature 382: 325–331

S.K.Walker L, Abbas AK. 2002. THE ENEMY WITHIN: KEEPING SELF-REACTIVE T CELLS AT BAY IN THE PERIPHERY. Nat. Rev. Immunol. 2: 11-9

Sato K, Balla J, Otterbein L, Smith RN, Brouard S, Lin Y, Csizmadia E, Sevigny J, Robson SC, Vercellotti G. 2001. Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants. J. Immunol. 166: 4185–4194

Scandella E, Men Y, Gillessen S, rster RFL, Groettrup M. 2002. Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells. Blood 100: 1354-61

Schnurr M, Then F, Galambos P, Scholz C, Siegmund B, Endres S, Eigler A. 2000. Extracellular ATP and TNF-alpha synergize in the activation and maturation of human dendritic cells. J. Immunol. 165: 4704-9

Serrano M. Hannon GJ. Beach D.1993. A new regulatory motif in cell cycle control causing specific inhibition of cyclinD/CDK4. Nature 366: 704–707.

Shortman K, Liu Y-J. 2002. MOUSE AND HUMAN DENDRITIC CELL SUBTYPES. Nat. Rev. Immunol. 2: 151-61

Smits HH, Jong EC, Wierenga EA, Kapsenberg ML. 2005. Different faces of regulatory DCs in homeostasis and immunity. Trends Immunol. 26: 123-9

Son Y-I, Egawa S-i, Tatsumi T, E. R, Jr. R, Kalinski P, Kanto T. 2002. A novel bulk-culture method for generating mature dendritic cells from mouse bone marrow cells. J. Immunol. Methods 262: 145-57

Song R, Mahidhara RS, Zhou Z, Hoffman RA, Seol D-W, Flavell RA, Billiar TR, Otterbein LE, Choi AMK. 2004. Carbon Monoxide Inhibits T Lymphocyte Proliferation via Caspase-Dependent Pathway. J. Immunol. 172: 1220–6

STOCKWIN LH, McGONAGLE D, MARTIN IG, BLAIR GE. 2000. Dendritic cells: Immunological sentinels with a central role in health and disease. Immunol. Cell Biol. 78: 91–102

Szabolcs P. Moore MA. Young JW. 1995. Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors cultured with c-Kit ligand, granulocyte macrophage colony-stimulating factor, and TNF-alpha. J. Immunol. 154: 5851-5861

Todd I. 2001. Cells of the Immune System. ENCYCLOPEDIA OF LIFE SCIENCES

Torti FM , Torti SV. 2002. Regulation of ferritin genes and protein. Blood 99: 3505–3516

Toyoshima H. Hunter T. 1994. p27, a novel inhibitor of G1 cyclin/cdk protein kinase activity, is related to p21. Cell 78: 67–74

Ulevitch RJ. 2000. Molecular Mechanisms of Innate Immunity. Immunol. Res. 21: 49-54

Vegh Z, Mazumder A. 2003. Generation of tumor cell lysate-loaded dendritic cells preprogrammed for IL-12 production and augmented T cell response. Cancer Immunol. Immunother. 52: 67-79

Verhasselt V, Vanden Berghe W, Vanderheyde N, Willems F, Haegeman G, Goldman M. 1999. N-acetyl-L-cysteine inhibits primary human T cell responses at the dendritic cell level: association with NF-kappaB inhibition. J. Immunol. 162: 2569-2574

Vremec D, Pooley J, Hochrein H, Wu L, Shortman K. 2000. CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen. J. Immunol. 164: 2978–86

Wesemann DR, Dong Y, O’Keefe GM, Nguyen VT, Benveniste EN. 2002. Suppressor of Cytokine Signaling 1 Inhibits Cytokine Induction of CD40 Expression in Macrophages. J. Immunol. 169: 2354–60

Whiteside TL, Odoux C. 2004. Dendritic cell biology and cancer therapy. Cancer Immunol. Immunother. 53: 240–8

Willoughby DA, Moore AR, Colville-Nash PR, Gilroy D. 2000. Resolution of inflammation. Int. J. Immunopharmacol. 22: 1131–1135

Woltman AM, Kooij SWvd, Coffer PJ, Offringa R, Daha MR, Kooten Cv. 2003. Rapamycin specifically interferes with GM-CSF signaling in human dendritic cells, leading to apoptosis via increased p27KIP1 expression. Blood 101: 1439-45

Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R and Beach D . 1993. p21 is a universal inhibitor of cyclin kinases. Nature 366: 701–704

Yang L, Quan S, and Abraham NG. 1999. Retrovirus-mediated HO gene transfer into endothelial cells protects against oxidant-induced injury. Am. J. Physiol. 277: L127–L133

Yu Y, Hagihara M, Ando K, Gansuvd B, Matsuzawa H, Tsuchiya T, Ueda Y, Inoue H, Hotta T, Kato S. 2001. Enhancement of Human Cord Blood CD34+ Cell-Derived NK Cell Cytotoxicity by Dendritic Cells. J. Immunol. 166: 1590–600

Zsuzsa Vegh, Amitabha Mazumder. 2003. Generation of tumor cell lysate-loaded dendritic cells preprogrammed for IL-12 production and augmented T cell response. Cancer Immunol. Immunother. 52: 67–79
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
1. 莊雅茹(民85):CAI軟體電腦動畫應用與學習成效分析。視聽教育雙月刊, 38(2), 9-16
2. 林君宜(民94):虛擬圖書館 改變知識流通路徑。商業周刊, 934, 144-146。
3. 林奇賢(民87):網路學習環境設計與應用。資訊與教育雜誌, 67, 34-49。
4. 沈中偉、胡志根(民81):圖書館視聽室的管理與服務。教學科技與媒體, 4, 3-17。
5. 沈中偉(民92)、余佩芬:多元化記憶策略電腦輔助教學對國小六年級學生社會學習領域配對學習之研究。視聽教育雙月刊, 44 (5) , 2-15。
6. 沈中偉(民87):即時群播遠距教學之教學設計與教學策略探討。遠距教育, 7, 13-19。
7. 陳佩正(民91):科學發展史的教學與學生的創意。應用心理研究, 15,
8. 張維安(民93):網路時代中孩童的電子邊疆。教育研究月刊, 118, 43-54
9. 張俊彥、翁玉華(民89):我國高一學生的問題解決能力與其科學過程技能之相關研究。科學教育學刊, 8(1), 35-56
10. 張靜嚳(民85):傳統教學有何不妥?建構與教學,4(1)。取自:http://scied.ncue.edu.tw/ct/v4-1.htm
11. 楊家興(民82):超媒體 一個新的學習工具。教學科技與媒體, 12, 28-39。
12. 詹志禹(民94):人類的創造力從何而來?科學人, 45, 39-40
13. 鍾蔚文(民94):創意是嚴肅的遊戲。科學人, 45 , 37-37
14. 饒見維(民90):九年一貫課程與教師專業角色的省思。教師天地, 113, 7-13。