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研究生:萬育仁
研究生(外文):yu-ren wan
論文名稱:金屬離子與合成聚乙醚精土複合物毒性分析研究
論文名稱(外文):The Toxicity Study of Metal Ion and Synthesized Polyether-Clay Compound
指導教授:李源弘
指導教授(外文):Yuan-Haun Lee
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:材料科學與工程學研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:77
中文關鍵詞:生醫材料麵包酵母菌劑量反應分析聚乙醚精土複合物
外文關鍵詞:biomaterialSaccharomyces cerevisiaedose-responseanalysissynthesized polyether-clay compound
相關次數:
  • 被引用被引用:1
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先前馮[2003]之研究(1)中發現,以麵包酵母菌(Saccharomyces cerevisiae CCRC 22049)作為藥物載體之蒙脫石(Montmorillonite))生物毒性評估,其與「蒙脫石對大鼠生物毒性實驗」結果均證明蒙脫石(Montmorillonite)基本上可視其為低毒性具可行性之生物材料,故本研究利用麵包酵母之生物毒性評估法檢測常見之一般生醫「植入」材料(如:F136 【Ti-6Al-4V】 、ASTM F75【Co-Cr-Mo】、F138 316L不鏽鋼【Fe-Ni-Cr】)以及合成之聚乙醚精土複合物(synthesized polyether-clay compound)為對象,並與文獻上之毒性結果作成份分析比較,以進一步判斷麵包酵母菌替代文獻中生物毒性評估法之可行性,再就新複合材料之毒理性作探討。研究首先,以合金中八種主要元素作單成分生物毒性分析,後續再就成份間交互作用(如: 協同作用【synergism】拮抗作用【antagonism】)以分別定義出主要毒性金屬及其對於合金成分之效應,並以毒理學上之「劑量反應分析」(dose-response analysis)方式作為研究評估分析之學理依據,試圖對生醫材料之安全與功能改善進行深入之探討。而後就合成之複合物作生物毒性分析結果探討。
Our previous study(1) indicated that the results of toxicity assay for montmorillonite as drug carrier in bacterial(Saccharomyces cerevisiae CCRC 22049) and rat model pointed out that the toxicity of the drug was very weak. Therefore, this study focused the toxicity of three popular biomaterials (example:ASTM F136 【Ti-6Al-4V】、ASTM F75【Co-Cr-Mo】、ASTM F138 316L stainless steel【Fe-Ni-Cr】) and synthesized polyether-clay compound in bacterial (Saccharomyces cerevisiae) assay. The toxicity of eight aqueous metal solutions was first deteremined. Then, aqueous metal solutions were then mixed according to the proportions of the elements found in these alloys, and their toxicity was measured with yeast. Mixture behavior was classified as synergistic or antagonistic by comparing measured toxicity to predicted toxicity. Then measured toxicity of biomaterial and synthesized polyether-clay compound was analyzed by dose-response analysis and compared with measured toxicity by Microtox(from reference(13)). In the result, the experiments demonstrate the safty and improvement of materials and discuss these.
頁數
中 文 摘 要 Ⅰ
英 文 摘 要 Ⅱ
目 錄 Ⅳ
第一章 緒 論 1
1.1研究目的 1
1.2 研究內容 2
第二章 文獻回顧與理論基礎 4
2.1 生醫材料毒理分析的模式 4
2.1.1 鐵鉻鎳合金(不�袗�)簡介 5
2.1.2 鈷鉻鉬合金簡介 6
2.1.3 鈦合金簡介 7
2.1.4 金屬離子對於人體的機能性 8
2.1.5 聚乙醚化合物 11
2.2 麵包酵母菌(Saccharomyces cerevisiae) 17
2.2.1 麵包酵母菌簡介 17
2.2.2微生物的生長模型 22
2.2.3非結構化之生長模 24
2.2.4麵包酵母菌應用於毒性分析(montmorillonite) 25
2.3毒理性分析方法 26
2.3.1 毒性反應分析(dose-response analysis) 26
2.3.2合金成份毒性預估公式及運算法 29
2.3.3 ISO 10993-5材料毒性試驗規範 30
2.3.4 Microtox毒理評估文獻整理 31
第三章 實驗儀器與實驗步驟 36
3.1實驗儀器 36
3.2實驗藥品 37
3.3實驗方法與流程 38
3.3.1酵母菌培養 38
3.3.2 pH值效應實驗 39
3.3.3金屬溶液配置 39
3.3.4酵母菌生長抑制實驗 40
3.3.5 陰離子對照實驗 41
第四章 結果與討論 43
4.1 pH值效應實驗 43
4.2 陰離子對照實驗 44
4.3酵母菌生長抑制實驗 52
4.4酵母菌模式與Microtox®之毒性結果比較 70
第五章 結論 71
附錄一 CF4之物理性質表 73
參 考 文 獻 74
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