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研究生:劉仲陽
研究生(外文):Chung-Yang Liu
論文名稱:利用螢光光譜法探測chitin/chitosan之去乙醯化程度與複合材料結構
指導教授:林金福林金福引用關係
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:高分子科學與工程學研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:93
語文別:中文
論文頁數:87
中文關鍵詞:幾丁聚醣戊二醛螢光
外文關鍵詞:glutaradehydechitosanfluorescence
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本研究是利用螢光光譜法探測幾丁質與幾丁聚醣在不同程度去乙醯化的螢光光譜變化,試著以即時、快速且簡單的方式判斷去乙醯化程度對幾丁聚醣應用上有極大影響,並且進一步尋找結構與螢光的關係。在本研究中,不同去乙醯化幾丁質以紅外光譜判斷去乙醯化程度並配合紫外光譜與螢光光譜觀察其固態下與溶液下吸收光譜與螢光光譜對不同去乙醯的變化。高去乙醯化的幾丁聚醣利用不同含量過硫酸鉀與幾丁聚醣之間的降解反應,探討分子鏈對螢光光譜的關係。以此螢光探測法為基礎,延伸螢光探測上的應用於以幾丁聚醣經蒙脫石改質並以不同程度之戊二醛進行交聯反應所作成的支架,利用螢光光譜法觀察其變化,以期在實際應用面上能以螢光光譜法方便的探測幾丁聚醣結構。
In this study, we used the change of fluorescence parameters ( maximum wavelength , intensity and intensity ratio) of chitosan to correlate with their degree of deacetylation determined by FTIR. Moreover , potassium persulfate (KPS) was employed to degrade the chitosan in acidified aqueous solutions and the results were also correlated with the fluorescence spectroscopy . Furthermore , the fluorescence intensity ratio
(550nm/500nm) of the scaffolds prepared by chitosan/montmorillonite nanocomposites were employed to investigate their structure crosslinked by various content of glutaradehyde.
摘要……………………………………………………………………I
Abstract…………………………………………………………… …Ⅱ
目錄……………………………………………………………………Ⅲ
第一章緒論.............................................. 1
第二章文獻回顧………………………………………………………4
2.1.幾丁聚醣……………………………………………………… 4
2.1-1幾丁質與幾丁聚醣的構造……………………………… 4
2.1-2 幾丁聚醣的性質………………………………………… 6
2.1-3 幾丁聚醣的應用………………………………………… 7
2.1-4 幾丁聚醣基質生醫材料……….……………………… 9
2.1-5 過硫酸鉀降解幾丁聚醣制………………………… 11
2.1-6 去乙醯化分析……………………………………… 13
2.2蒙脫石………………………………………………………… 14
2.2-1 蒙脫石理論構造……………………………………… 14
2.2-2 蒙脫石應用…………………………………………… 15
2.3螢光探測………………………………………………… 15
2.3-1 螢光原理……………………………………………… 15
2.3-2 螢光測量……………………………………………… 17
2.3-3 螢光光譜分析法的應用……………………………… 19
2.4研究方向……………………………………………………… 20
第二章 實驗方法……………………………………………………21
3.1實驗藥品與材料…………………………………………. 21
3.2實驗設備與儀器………………………………………… 22
3.3 實驗方法與步驟………………………………………… 24
3.3-1不同程度去乙醯化幾丁質製備……………………… 24
3.3-2 固態幾丁聚醣螢光樣本製備……………………… 24
3.3-3 溶液幾丁聚醣螢光樣本製備……………………… 24
3.3-4 過硫酸鉀降解幾丁聚醣樣本製備…………… 25
3.3-5 蒙脫石的改質…………………………………… 25
3.3-6 以2wt%製備之幾丁聚醣/過硫酸鉀-蒙脫石複合材料支架製備……………………………………… 26
3.3-7 紅外光光譜分析……………………………… 27
3.3-8 紫外光光譜分析…………………………………… 28
3.3-9 螢光光譜分析……………………………………… 28
第四章 結果與討論………………………………………………… 29
4.1 幾丁質/幾丁聚醣固態、溶液螢光光譜比較…………… 30
4.2 不同程度之去醯化紅外光譜測定……………………… 30
4.3 不同去醯化程度幾丁質固態螢光光譜測定…………… 31
4.4 不同去醯化程度幾丁質液態紫外光/螢光光譜測定…… 31
4.5 不同含量過硫酸鉀降解幾丁聚醣紅外光譜測定.32
4.6 不同含量過硫酸鉀降解幾丁聚醣螢光譜測定…. 34

4.7 不同含量戍二醛交聯幾丁聚醣複材螢光光譜測定……………………………………………………… 36
4.7-1 不同含量戊二醛交聯幾丁聚醣支架螢光光譜測定…………………………………………… 36
4.7-2 不同含量戊二醛交聯幾丁聚醣複材支架螢光光譜測定……………………………………… 37
第五章 結論.............................................40
第六章 參考文獻.........................................42
第六章 參考文獻
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