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研究生:張家豪
研究生(外文):Chia-Hao Chang
論文名稱:聚四氟乙烯及聚甲基丙烯酸甲酯之改質與生物相容性評估及運用
論文名稱(外文):Modification of PTFE and PMMA for biocompatibility evaluation and their applications
指導教授:楊台鴻
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:高分子科學與工程學研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:83
中文關鍵詞:小腦顆粒型神經細胞聚四氟乙烯丁基鋰推電子溶劑親疏水性細胞圖案微流道去氟反應烷化反應
外文關鍵詞:cerebellum cortical granule cellspolytetrafluoroethenebutyllithiumelectron-donating solventhydrophobicitycellular patternmicrofluidic channeldefluorinationalkylation
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本實驗利用丁基鋰化合物在不同推電子溶劑(乙二胺、丁二胺及乙胺)催化下,改質高分子聚四氟乙烯(PTFE),使其產生去氟烷化反應;利用FTIR-ATR、ESCA分析改質前後表面化學組成差異並測量接觸角以判斷親疏水性變化。
同時利用體外細胞培養的方法,來探討顆粒型神經細胞在改質前後PTFE的適合性,並且利用微流道技術設計細胞圖案,使PTFE產生親疏水性差異的表面。結果使用金相顯微鏡及掃描式電子顯微鏡觀察細胞生長。由結果得知,未改質前細胞無法貼附生長,在經由改質後之PTFE表面可以發現細胞群聚成球且生長突觸的現象。
In this study, butyllithium was catalyzed by different electron-donating solvents (ethyldiamine, butyldiamine and ethylamine) for defluorination and alkylation to modify PTFE. FTIR-ATR and a ESCA results were used to analyze the composition of unmodified / modified surfaces and contact angles were measured to distinguish the variation of hydrophobicity.
In vitro cerebellum cortical granule cells culturing was then used to test the biocompatibility of PTFE before and after modification, Microfluidic channels were also used to produce differential hydrophilic surfaces for cell patterning. Finally, a Metallurgical Microscope and a SEM were used to observe cell growing. According to the experimental results, cells could not adhere to unmodified PTFE surfaces. On the other hand, small populations of cells and synapse extension were observed on the modified PTFE surface.
第一章 緒論.................... 1
1-1 前言...................... 1
1-2 神經細胞簡介.................. 3
1-2-1 影響神經細胞生長的因素........... 4
1-3 細胞圖案簡介.................. 6
1-4 PTFE簡介................... 8
1-4-1 PTFE化學改質簡介............... 9
第二章 實驗流程與步驟............... 11
2-1 實驗架構.................... 11
2-2 實驗藥品..................... 12
2-3 實驗儀器..................... 15
2-4 PTFE表面改質................... 19
2-4-1實驗設備.................... 21
2-4-2實驗步驟................... 22
2-5 細胞圖案..................... 23
2-5-1 實驗裝置.................... 23
2-6 材料表面鑑定................... 24
2-6-1 接觸角實驗(Contact Angle)........... 24
2-6-2衰減全反射傅立葉紅外光譜儀(ATR-FTIR)...... 25
2-6-3化學分析電子光譜(ESCA)............. 26
2-7 神經細胞培養.................. 27
2-7-1 試藥配製.................... 28
2-7-2 材料前處理................... 31
2-7-3 塗佈聚離胺酸.................. 31
2-7-4顆粒型神經細胞初代培養............. 32
2-8 掃描式電子顯微鏡觀察(SEM)............ 33
2-8-1 細胞固定(Fixation)............... 34
2-8-2 臨界點乾燥(Critical Point Dryer)........ 35
2-8-3 樣品鍍金.................... 36
第三章 結果與討論................. 37
3-1 PTFE表面改質分析................. 37
3-1-1 接觸角實驗(Contact Angle)............37
3-1-2衰減全反射傅立葉紅外光譜儀(ATR-FTIR)...... 38
3-1-3化學分析電子光譜(ESCA)............. 40
3-2 神經細胞培養................... 42
3-2-1顆粒型神經細胞初代培養............. 42
3-3 細胞圖案..................... 43
第四章 結論.................... 44
第五章 未來展望.................. 45
第六章 參考文獻.................. 47
附錄........................ 66
1.Ravi Bellamkonda, Patrick Aebischer, “Review:tissue engineering in the nervous system,” Biotechnol. bioeng, 43(1994), 543-554
2.Chen Y.S., Hsieh C.L., Tsai C.C., Chen T.H., Cheng W.C., Hu C.L., Yao C.H., “Peripheral nerve regeneration using silicone rubber chambers filled with collagen, laminin, & fibronectin,” Biomaterials, 21(2000), 1541-1547
3.Woerly S., Morassutti D.J., “New aspects of neuroplantation,” Neurosurg Rev., 16(1993), 93-104
4.Gerald Karp, “Cell and molecular biology,” John Wiley and Sons, Inc. New York, USA, (2002)
5.Vellamkonda R., Raneri J.P., Bouche N., Arbischer P., “Hydrogel -based three-dimensional matrix for neural cell,” J. Biomed. Mater. Res., 29(1995), 663-671
6.Aebischer P., Valentini R.F., Dario P., Domenici C., Galletti P.M., “Piezoelectric guidance channels enhance regeneration in the mouse sciatic nerve after axotomy,” Brain Res., 436(1987),165-168
7.Dunnen W.F.A., Lei B.V.D., Schakenraad J.M., Stokroos I., Pennings A.J., Robinson P.H., “Long-term evaluation of nerve regeneration in a biodegradable nerve guide,” Microsurg., 14(1993), 508-515


8.Borkenhagen M., Stoll R.C., Neuenschwander P., Suter U.W., Aebischer P., “In vivo performance of a new biodegradable polyester urethane system used as a nerve guidance channel,” Biomaterials, 19(1998), 2155-2165
9.Woerly S., Marchand R., “Intracerebral implantation of synthetic polymer/biopolymer matrix: a new perspective for brain repair,” Biomaterials, 11(1990), 97-107
10.Harsch J. Calderon R.B., Timmons G.W., “Pulsed plasma deposition of allyamine on polysiloxane: a stable surface for neuronal cell adhesion,” J. Neurosci. Meth. 98(2000), 135-144
11.R. F. Valentini, A. M. Sabatini, P. Dario, P. Aebischer, “Polymer electret guidance channels enhance peripheral nerve regeneration in mice,” Brain res., 480(1989), 300-304
12.Sergio Martinoia, Marco Bove, Mariateresa Tedesco, Benno Margesin, Massimo Grattarola, “A simple microfluidic system for patterning populations of neurons on silicon micromachined substrates,” J. neurosci. methods, 87(1999), 35-44
13.Milan Mrksich, Laura E. Dike, Joe Tien, Donald E. Ingber, George M. Whitesides, ”Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver,” Exp. cell res., 235(1997), 305-313
14.Zhang S, Yan L, Altman M, Lassle M, Nugent H, Frankel F, Lauffenburger DA, Whitesides GM, Rich A., “Biological surface engineering: a simple system for cell pattern formation,” Biomaterials, 20(1999), 1213-1220
15.Degenaar P, Pioufle BL, Griscom L, Tixier A, Akagi Y, Morita Y, Murakami Y, Yokoyama K, Fujita H, Tamiya E., “A method for micrometer resolution patterning of primary culture neurons for SPM analysis,” J.Biochem. 130(2001), 367-376
16.Hirokazu Kaji, Kazuto Tsukidate, Tomokazu Matsue, and Matsuhiko Nishizawa,, “In Situ Control of Cellular Growth and Migration on Substrates Using Microelectrodes,” J. AM. CHEM. SOC., 126(2004), 15026-15027
17.Charles E. Carraher, Jr. ”Polymer Chemistry,” Marcel Dekker, Inc., New York, (2000)
18.Jiri Jansta, Frantisek P. Dousek, ”Electrochemical corrosion of polytetrafluorethylene contacting lithium amalgam,” Electrochim. acta., 18(1973) 673-674
19.J. Jansta, F.P. Dousek, J.Riha, J.Heyrovsky, “Quantitative explanation of the mechanism of corrosion of poly(tetrafluoroethylene) caused by active alkali metals,” J. appl. polym. sci., 19(1975), 3201-3210
20.Ladislav Kavan, Zdenek Bastl, Frantisek P. Dousek, Jiri Jansta, “XPS study of carbon in electrochemical reduction products of poly(tetrafluoroethylene),” Carbon, 22(1984), 77-81
21.L. KAVAN, P. JANDA, J. WEBER, “Surface modification of poly(tetrafluoroethylene) by magnesium amalgam,” J. mater. sci., 36(2001), 879-885



22.Yasuhiro Okuda, Fumihiro Hayashi, Hiroshi Sakurai, Masaru Shiotani, ”A spectroscopic study on defluorination of poly (tetrafluoroethylene) by alkyllithium/electron-donating solvents,” Spectrochim. Acta. Part A. 60(2004) 3071-3077
23.Jaromir Hlavaty, Ladislav Kavan, Zdenek Bastl, “Stimultaneous fluorine elimination and alkylation of poly(tetrafluoroethylene) ,” Angew. Macrom. Chem. 238(1996) 165-175
24.Thomas A. Zwier, Michael F. Burke, “Chemical Modification of a Carbon Adsorbent for Liquid Chromatography, “Anal. Chern., (53)1981, 812-816
25.Anthony J. Dias and Thomas J. McCarthy, “Surface Modification of Poly(chlorotrifluoroethy1ene) with Methyllithium,” Macromolecules, 18(1985), 1826-1829
26.Nilanjan Chakrabarti, John Jacobus, “The Chemical Reduction of Poly(tetrafluoroethy1ene) ,” Macromolecules, 21(1988), 3011-3014
27.Christine A. Costello, Thomas J. McCarthy, “ Surface Modification of Poly(tetrafluoroethylene) with Benzoin Dianion,” Macromolecules, 17(1984), 2940-2942
28.Hiroshi Sakurai, Yoshihiro Kubo, Masaru Shiotani, Hidenori Yahiro, Yasuhiro Okuda, “Reduction of Poly(tetrafluoroethylene) by Aromatic RadicalAnions: An ESR and NMR Study,” J. appl. polym. sci., 74(1999), 286-289



29.A. M. Dessouki, N. H. Taher, M. B. El-Arnaouty, “Gamma Ray Induced Graft Copolymerization of N-Vinylpyrrolidone, Acrylamide and Their Mixtures Onto Polypropylene Films,” Polymer International, 45 (1998), 67-76
30.El-Sayed A. Hegazy, N. H. Taher, H. Kamal, “Cationic Membranes From PTFE-g-PMAA,” J. appl. polym. sci., 38(1989), 1229-1242
31.D. J. Wilson, R. L. Williams, R. C. Pond, “Plasma modification of PTFE surfaces,” Surf. Interface Anal., 31(2001), 385-396
32.V. SVORCIK, I. MICEK, V. RYBKA, L. PALMETSHOFER, V. HNATOWICZ, “Ion Beam Ablation of Polytetrafluoroethylene,” J. appl. polym. sci., 69(1999), 1257 - 1261
33.J. Heitz, V. S vorcık, L. Bacakova, K. Rockova, E. Ratajova, T. Gumpenberger, D. Bauerle, B. Dvorankova, H. Kahr, I. Graz, C. Romanin, “Cell adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere,” J. biomed. materi. res., 67(2003), 130-137
34.SUNG R. KIM, “Surface Modification of Poly(tetrafluoroethylene) Film by Chemical Etching, Plasma, and Ion Beam Treatments,” J. appl. polym. sci., 77(2000), 1913-1920
35.Masashi Miwa, Akira Nakajima, Akira Fujishima, Kazuhito Hashimoto, Toshiya Watanabe, “Effects of the surface roughness on sliding angles of water droplets on superhydrophobic surface,” Langmuir 16(2000), 5754-5760


36.John P. LaFemina, “Surface analytical techniques for probing biomaterial processes,” Edited by John Davies et al., CRC Press, Inc. Boca Raton, USA, (1996)
37.Donald L. Pavia, Gary M. Lampman, George S. Kriz, “Introduction to spectroscopy,” Harcourt, Inc. ,Orlando, (2001)
38.Chittur, k. k., “FTIR/ATR for protein adsorption to biomaterial surfaces,” Biomaterials, 19(1998), 357-369
39.Hari Singh Nalwa, “Advances In Surface Science,” Academic press., London, (2001)
40.Santosh. R. D’Mello, Cinzia. Galli, Teresa. Ciotti, Pietro. Calissano, “Induction of apotosis in cerebellar granule neurons by low potassium: Inhibition of death by insulin-like growth factor I and CAMP,” Proc. Natl. Acad. Sci. 90(1993), 10989-10993
41.Oliver C. Wells, “Scanning electron microscopy,” McGraw-Hill, Inc., USA
42.黑田行昭原著,上野洋一郎, 溫秋明, 柯俊良, 陳怡菁, 郭順宇, 郭榮烈, 陳泓志, 胡威文, 張傳偉編譯 “組織培養的技術” , 藝軒圖書出版社,台北縣, 台灣, (2002)
43.L. Kavan, J. Kastner, “Carbyne forms carbon:continuation of the story, ”Carbon, 32(1994), 1533-1536
44.J.Kastner, H.Kuzmany, L.Kavan, F.P.Dousek, J.Kurti, “Reductive preparation of carbine with high yield. An raman scattering study, ”Macromolecules, 28(1995), 344-353
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2. 李坤崇(2002)。多元化教學評量理念與推動策略。教育研究,98,24-35頁。
3. 方炎明(1989)。近四十年來我國民中小學之科學教育。教育資料集刊第十四輯。台北市:國立資料教育館。
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5. [17]許道然(2002)。組織公民行為之研究。空大行政學報,12期,114-145頁。
6. [24]黃英忠、余德成(1989)。 工作滿足相關因素之研究:以楠梓加工出口區女性員工為例。管理科學學報,10(1)期,157–178頁。
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12. [9]汪芳興、劉漢文(2002),非晶矽TFT-LCD製造與畫素設計,電子月刊,8(8),102-109。
13. [15]洪維宗、陳景榮(2004),灰色關聯分析於整合田口方法多重品質特性最佳化-在食品工業製程實證,東海學報,45(10),69-88。
14. [18]莊榮仁(2001),田口式品質工程動態特性及其在化工技術開發上的應用例,化工技術,9(4),284-291。
15. [27]楊素芬、郭昭貝(2003),以田口方法改善陀螺旋轉之持久性,品質月刊,39(6),75-79。
 
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