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研究生:李育宗
研究生(外文):Yu-Tsung Lee
論文名稱:豬增生性壞死性肺炎病灶與常見病毒包括第二型豬環狀病毒、豬生殖與呼吸道綜合症病毒、豬流行性感冒病毒及豬小病毒之關聯性
論文名稱(外文):The Role of Viral Infection on the Development of Porcine Proliferative and Necrotizing Pneumonia-Like Lesions: PCV2, PRRSV, SIV, and PPV
指導教授:鄭謙仁
指導教授(外文):Chian-Ren Jeng
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:獸醫學研究所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:111
中文關鍵詞:豬增生性壞死性肺炎第二型豬環狀病毒豬生殖與呼吸道綜合症病毒豬流行性感冒病毒豬小病毒
外文關鍵詞:Porcine Proliferative and Necrotizing PneumoniaPorcine Circovirus type 2Porcine Reproductive and Respiratory Syndrome VirusSwine Influenza VirusPorcine Parvovirus
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豬增生性壞死性肺炎(Porcine proliferative and necrotizing pneumonia; PNP)最早於1988年加拿大魁北克省發現。顯微鏡下特徵為第Ⅱ型肺泡細胞增生,肺間質變厚且肺泡腔有許多單核細胞及脫落壞死細胞之蓄積,偶爾可見玻璃樣膜之形成及細支氣管壞死;因其顯微病灶呈現特異變化,且不明瞭是由何種病原所引起,故稱之為“豬增生性壞死性肺炎”。 本研究自台灣地區豬場,於臨床呈現呼吸急促、呼吸困難及腹式呼吸等症狀之患豬,從2002至2003年共收集275個病例。以組織病理學進行形態變化之篩選,發現有50個(18%)病例呈現增生性壞死性肺炎之形態;於此50個PNP病例,發現細菌與病毒混合感染病例占49例(98%),僅有1例(2%)為單一病毒感染所致。以聚合酶鏈鎖反應(PCR)偵測第二型豬環狀病毒(PCV2)、豬生殖與呼吸道綜合症病毒(PRRSV)及黴漿菌(Mycoplasma hyopneumoniae)分別有50(100%)、6(12%)及11(22%)個陽性病例;另外細菌分離以沙氏桿菌(Salmonella sp.)所占比例最高,約為22.4%。另運用原位雜交法(ISH)對PCV2、PRRSV及豬流行性感冒病毒(SIV)進行檢測,結果發現PCV2主要分佈於PNP的間質及壞死病灶區,並能於肺泡及終末細支氣管上皮細胞、巨噬細胞與壞死細胞碎片皆可見有病毒核酸;PRRSV則多分佈於間質病灶區及肺泡壁處,能於巨噬細胞與肺泡上皮細胞見到病毒核酸;SIV則存在於間質及壞死病灶區,且能於肺泡及終末細支氣管上皮細胞與巨噬細胞見有病毒核酸。另外,以免疫組織化學染色(IHC)檢測豬小病毒(PPV),其陽性訊號主要分佈於間質及壞死病灶區,此陽性訊號存於巨噬細胞與肺泡及終末細支氣管上皮細胞。由ISH結果顯示,PCV2與SIV兩種病毒多於壞死及間質病灶區見有病毒核酸,但SIV呈現之核酸訊號較少且弱。然而,PRRSV主要分佈位置與間質病灶區之肺泡壁較具有關聯性。PPV常於壞死及間質病灶區內見有陽性訊號,但亦能於終末細支氣管之化膿病灶區見有陽性訊號。以ISH及IHC探討四種不同病毒之分佈位置得知,PNP病灶應由不同病原混合感染所造成;其中又以PCV2及PPV兩種病毒最為重要,此兩種病毒之分佈與PNP病灶位置具有緊密之關聯性。另外,本實驗首次發現台灣地區豬隻具有高盛行率之PPV感染。
In the late 1980s, a new type of porcine pneumonia of unknown etiology called proliferative and necrotizing pneumonia (PNP) was described in Quebec, Canada. Histologically, it was characterized by a lymphohistiocytic interstitial pneumonia with a marked proliferation of type 2 pneumocytes and many coagulates of necrotic cells in the alveoli. Occasionally, hyaline membranes and bronchiolar necrosis were also observed. Between 2002 and 2003, 275 cases were collected from pig farms of Taiwan. The main clinical signs of these cases were tachypnoea, dyspnea, and abdominal respiration. Histopathological characteristic lesions of proliferative and necrotizing pneumonia (PNP) were found in 50 cases (18%). Single viral infection was only detected in 1 case (2%), whereas in other 49 cases (98%), a combination of viral and bacterial infection was noted. Polymerase chain reaction (PCR) was used for the detection of porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae, and the frequency of positive results were 50 (100%), 6 (12%) and 11 (22%) cases, respectively. The infection of Salmonella sp. was presented in 12 cases (24%) by bacterial isolation. By in-situ hybridization (ISH), PCV2 signals were chiefly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and necrotic debris. PRRSV signals were mainly located at interstitial lesions and alveolar wall, and the positive signals could be found in macrophages-like cells and epithelial cells of alveoli. Swine influenza virus (SIV) could be found in interstitial and necrotizing lesions, and the signals could be found in macrophages-like cells, and epithelial cells of terminal bronchiole and alveoli. By immunohistochemistry (IHC), porcine parvovirus (PPV) signals were mainly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and epithelial cells of terminal bronchiole and alveoli. The ISH results revealed that the signals of PCV2 and SIV were profoundly present in the necrotic and interstitial lesions, but SIV signals were weaker and less often. The signals of PRRSV were scattered and related to the alveolar wall of interstitial lesions. The IHC results showed the signals of PPV were present in the necrotic and interstitial lesions, the signals could occasionally be found in suppurative lesions of the terminal bronchiole. In conclusion, PNP lesions maybe induced by the co-infection of several pathogens, especially PCV2 and PPV. Additionally, this is the first study demonstrates a high prevalence of PPV infection in Taiwan.
目錄…………………………………………………………………………Ⅰ
表次…………………………………………………………………………Ⅴ
圖次…………………………………………………………………………Ⅵ
中文摘要……………………………………………………………………Ⅶ
英文摘要……………………………………………………………………Ⅸ
第一章 序言………………………………………………………………...1
第二章 文獻回顧……………………………………………………………3
第一節 肺臟的解剖生理及病理變化之簡介…………………………3
1-1 前言…………………………………………………………...3
1-1-1 肺臟的一般正常結構、形態與生理功能……………..3
1-1-1-1 肺內導氣部………………………………………...4
1-1-1-2 肺的呼吸部………………………………………...4
1-1-2 肺臟組織的再生與衰老變化…………………………..6
1-2 肺炎之分類、組織病理學型態及可能的特殊致病機制……6
1-2-1 支氣管性肺炎…………………………………………..7
1-2-2 間質性肺炎……………………………………………..7
1-2-3 栓子性肺炎……………………………………………..8
1-2-4 肉芽腫性肺炎…………………………………………..9
第二節 豬增生性壞死性肺炎………………………………………...10
2-1 歷史背景與發生概況………………………………………..10
2-2 豬增生性壞死性肺炎之臨床症狀與病理變化……………..10
第三節 豬增生性壞死性肺炎相關病原之特性………………………12
3-1 豬流行性感冒病毒之特性及病理變化……………………..12
3-1-1 豬流行性感冒病毒之特性、型態及構造…………….12
3-1-2 豬流行性感冒病毒之複製…………………………….13
3-1-3 豬流行性感冒病毒之流行病學……………………….13
3-1-4 豬流行性感冒病毒之臨床症狀與病理變化………….14
3-2 豬生殖與呼吸道綜合症病毒之特性及病理變化…………..15
3-2-1 豬生殖與呼吸道綜合症病毒之特性、型態及構造….15
3-2-2 豬生殖與呼吸道綜合症病毒之複製…………………..16
3-2-3 豬生殖與呼吸道綜合症病毒之流行病學……………..16
3-2-4 豬生殖與呼吸道綜合症病毒之臨床症狀與病理變化..17
3-3豬環狀病毒之特性與病理變化……………………………….18
3-3-1 豬環狀病毒之特性、型態及構造………………………18
3-3-2 豬環狀病毒之複製……………………………………...19
3-3-3 豬環狀病毒之流行病學………………………………...20
3-3-4 豬環狀病毒之臨床症狀與病理變化…………………...20
3-4 豬小病毒之特性與病理變化………….……………………...22
3-4-1 豬小病毒之特性、型態及構造…………………………22
3-4-2 豬小病毒之複製………………………………………...23
3-4-3 豬小病毒之流行病學…………………………………...24
3-4-4 豬小病毒之臨床症狀與病理變化……………………...24
3-5相似於豬增生性壞死性肺炎組織病灶之動物或人之疾病….25
3-5-1 Severe acute respiratory syndrome…………...................25
3-5-2 Acute respiratory distress syndrome…………………….26
3-5-3 Acute bovine pulmonary edema and emphysema………26
第四節 原位雜交……………………………………………………….27
第五節 免疫組織化學染色…………………………………………….29
第三章 材料與方法………………………………………………………….30
第一節 實驗設計……………………………………………………….30
第二節 實驗材料……………………………………………………….31
2-1 病材……………………………………………………………31
第三節 實驗方法……………………………………………………….31
3-1 病原診斷………………………………………………………31
3-2 組織病理學診斷………………………………………………32
3-3 豬隻基本資料與診斷之彙整…………………………………32
3-4 探針之製備……………………………………………………32
3-4-1 PCV2探針………………………………………………32
3-4-2 PRRSV探針…………………………………………….33
3-4-2-1 微量質體DNA萃取……………………………….33
3-4-2-2 聚合酶鏈鎖反應…………………………………...33
3-4-2-3 DNA洋菜膠片電泳分析………………………….34
3-4-2-4 PCR產物的直接核苷酸定序……………………..35
3-4-2-5 PRRSV探針之合成……………………………….35
3-4-3 SIV探針………………………………………………...35
3-4-3-1 流行性感冒病毒核酸之萃取……………………...35
3-4-3-2 反轉錄聚合酶鏈鎖反應…………………………...36
3-4-3-3 DNA洋菜膠片電泳分析…………………………..37
3-4-3-4 RT-PCR產物的直接核苷酸定序………………….37
3-4-3-5 SIV探針之合成……………………………………37
第四節 免疫膜點法…………………………………………………….37
第五節 原位雜交……………………………………………………….38
第六節 免疫組織化學染色…………………………………………….39
第四章 結果………………………………………………………………….41
第一節 實驗動物之背景、病原診斷與組織病理型態學之結果…….41
1-1 病原診斷……………………………………………………...41
1-2 組織病理型態學之分類……………………………………...41
第二節 增生性壞死性肺炎病例之組織學變化、病原診斷與年齡分佈情形……………………………………………………………45
2-1組織病理學之變化及特徵……………………………………45
2-2利用特殊染色排除此病灶為細菌團塊或鈣化現象…………45
2-3病原診斷結果及交叉分析……………………………………48
2-4罹病豬隻年齡分佈情形………………………………………48
第三節 探針製備結果………………………………………………….51
3-1 PRRSV之探針製備…………………………………………...51
3-2 AIV之探針製備……………………………………………….51
第四節 原位雜交及免疫組織化學染色結果………………………….56
4-1 PCV2原位雜交結果…………………………………………..56
4-2 PRRSV原位雜交結果………………………………………...63
4-3 SIV原位雜交結果…………………………………………….68
4-4 PPV免疫組織化學染色結果………………………………….73
4-5各病毒於PNP病灶區的相關位置及感染細胞……………....81
第五章 討論………………………………………………………………….83
第六章 參考文獻…………………………………………………………….97
附錄………………………………………………………………………….106
附錄一 50個PNP病例以PCR / RT-PCR、細菌分離與鑑定、原位雜交反應及免疫組織化學染色結果……………………………………….106
附錄二 50個PNP病例以PCR / RT-PCR、細菌分離與鑑定及組織病理學診斷結果…………………………………………………………….110
張志誠、鍾文彬、林敏雯、翁仲男、楊平政、邱雲棕、張文發、朱瑞民。1993。台灣地區豬繁殖與呼吸道症候群 Ⅰ. 病毒分離。中華獸醫誌。19: 268-276。
邱明堂、蘇保誠、莊孟修、林昭男。2004。台灣地區罹病豬隻中第二型豬環狀病毒感染狀況之調查。台灣獸醫誌。30: 163-168。
楊平政、張志成、朱瑞民。1996。豬病診療。台灣養豬科學研究所。苗栗縣。台灣。
劉振軒、王綉真、蘇豐振、傅千惠。1997。改良式免疫組織化學染色技術應用於福馬林液固定與石蠟包埋豬組織之研究。中華獸醫誌。23:523-530。
Astell, C.R., Chow, M.B., Ward, D.C., 1985, Sequence analysis of the termini of virion and replicative forms of minute virus of mice DNA suggests a modified rolling hairpin model for autonomous parvovirus DNA replication. J Virol 54, 171-177.
Allan, G.M., Ellis, J.A., 2000, Porcine circoviruses: a review. J Vet Diagn Invest 12, 3-14.
Allan, G.M., McNeilly, E., Kennedy, S., Meehan, B., Moffett, D., Malone, F., Ellis, J., Krakowka, S., 2000, PCV-2-associated PDNS in Northern Ireland in 1990. Porcine dermatitis and nephropathy syndrome. Vet Rec 146, 711-712.
Allan, G.M., McNeilly, F., Cassidy, J.P., Reilly, G.A., Adair, B., Ellis, W.A., McNulty, M.S., 1995, Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet Microbiol 44, 49-64.
Allan, G.M., McNeilly, F., Ellis, J., Krakowka, S., Meehan, B., McNair, I., Walker, I., Kennedy, S., 2000, Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication. Arch Virol, 145, 2421-2429.
Allan, G.M., McNeilly, F., Kennedy, S., Daft, B., Clarke, E.G., Ellis, J.A., Haines, D.M., Meehan, B.M., Adair, B.M., 1998, Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Invest 10, 3-10.
Aughey, E., Frye, F.L., 2001, Comparative veterinary histology with clinical correlates., Manson, London, 82-96 pp.
Baron, T., Albina, E., Leforban, Y., Madec, F., Guilmoto, H., Plana Duran, J., Vannier, P., 1992, Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation. Ann Rech Vet 23, 161-166.
Berns, K.I., 1996. Parvoviridae: the viruses and their replication. In: Fields, B.N., Knipe, D.M., Howley, P.M., Chanock, R.M., Melnick, J.L., Monath, T.P., Roizman, B. and Straus, S.E., Editors, 1996. Fields Virology (third ed.),, Lippincott-Raven Publishers, Philadelphia, pp. 2173–2197.
Bikour, M.H., Cornaglia, E., Weber, J.M., Elazhary, Y., 1994, Antigenic characterization of an H3N2 swine influenza virus isolated from pigs with proliferative and necrotizing pneumonia in Quebec. Can J Vet Res 58, 287-290.
Bilodeau, R., Archambault, D., Vezina, S.A., Sauvageau, R., Fournier, M., Dea, S., 1994, Persistence of porcine reproductive and respiratory syndrome virus infection in a swine operation. Can J Vet Res 58, 291-298.
Brigati, D.J., Myerson, D., Leary, J.J., Spalholz, B., Travis, S.Z., Fong, C.K., Hsiung, G.D., Ward, D.C., 1983, Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology 126, 32-50.
Brown, E.G., 2000, Influenza virus genetics. Biomed Pharmacother 54, 196-209.
Caron, J., Ouardani, M., Dea, S., 2000, Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in pigs by PCR amplification of the p36 and p46 genes. J Clin Microbiol 38, 1390-1396.
Cheon, D.S., Chae, C., 1999, Distribution of a Korean strain of porcine reproductive and respiratory syndrome virus in experimentally infected pigs, as demonstrated immunohistochemically and by in-situ hybridization. J Comp Pathol 120, 79-88.
Cheung, A.K., Bolin, S.R., 2002, Kinetics of porcine circovirus type 2 replication. Arch Virol 147, 43-58.
Cheung, O.Y., Chan, J.W., Ng, C.K., Koo, C.K., 2004, The spectrum of pathological changes in severe acute respiratory syndrome (SARS). Histopathology 45, 119-124.
Choi, C., Chae, C., 2000, Distribution of porcine parvovirus in porcine circovirus 2-infected pigs with postweaning multisystemic wasting syndrome as shown by in-situ hybridization. J Comp Pathol 123, 302-305.
Choi, C., Ha, S.K., Chae, C., 2004, Detection and isolation of H1N1 influenza virus from pigs in Korea. Vet Rec 154, 274-275.
Chueh, L.L., Lee, K.H., Jeng, C.R., Pang, V.F., 1999, A sensitive fluorescence in situ hybridization technique for detection of porcine reproductive and respiratory syndrome virus. J Virol Methods 79, 133-140.
Clark, E.G., 1996, Pathology of post-weaning multisystemic wasting syndrome of pigs. PROC WEST CAN ASSOC SWINE PRACT 22-25.
Conzelmann, K.K., Visser, N., Van Woensel, P., Thiel, H.J., 1993, Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology 193, 329-339.
Cotmore, S.F., Tattersall, P., 1992, In vivo resolution of circular plasmids containing concatemer junction fragments from minute virus of mice DNA and their subsequent replication as linear molecules. J Virol 66, 420-431.
Cox, M.A., Jenh, C.H., Gonsiorek, W., Fine, J., Narula, S.K., Zavodny, P.J., Hipkin, R.W., 2001, Human interferon-inducible 10-kDa protein and human interferon-inducible T cell alpha chemoattractant are allotopic ligands for human CXCR3: differential binding to receptor states. Mol Pharmacol 59, 707-715.
Crowther, R.A., Berriman, J.A., Curran, W.L., Allan, G.M., Todd, D., 2003, Comparison of the structures of three circoviruses: chicken anemia virus, porcine circovirus type 2, and beak and feather disease virus. J Virol 77, 13036-13041.
Curran, R.C., 2000, COLOUR ATLAS OF HISTOPATHOLOGY, 4th Edition. Harvey Miller, London, pp 212.
Dagerlind, A., Friberg, K., Bean, A.J., Hokfelt, T., 1992, Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry. Histochemistry 98, 39-49.
Dea, S., Bilodeau, R., Sauvageau, R., Montpetit, C., Martineau, G.P., 1992, Antigenic variant of swine influenza virus causing proliferative and necrotizing pneumonia in pigs. J Vet Diagn Invest 4, 380-392.
Dellmann, H.D., 1993, TEXTBOOK OF VETERINARY HISTOLOGY, 4th Edition. Lea & Febiger, PA, 144-152 pp.
Done, S.H., Paton, D.J., 1995, Porcine reproductive and respiratory syndrome: clinical disease, pathology and immunosuppression. Vet Rec 136, 32-35.
Doster, A.R., Mitchell, F.E., Farrell, R.L., Wilson, B.J., 1978, Effects of 4-ipomeanol, a product from mold-damaged sweet potatoes, on the bovine lung. Vet Pathol 15, 367-375.
Drolet, R., Larochelle, R., Morin et al., M., Delisle, B., Magar, R., 2003, Detection rates of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and swine influenza virus in porcine proliferative and necrotizing pneumonia. Vet Pathol 40, 143-148.
Drosten, C., Gunther, S., Preiser, W., van der Werf, S., Brodt, H.R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R.A., Berger, A., Burguiere, A.M., Cinatl, J., Eickmann, M., Escriou, N., Grywna, K., Kramme, S., Manuguerra, J.C., Muller, S., Rickerts, V., Sturmer, M., Vieth, S., Klenk, H.D., Osterhaus, A.D., Schmitz, H., Doerr, H.W., 2003, Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 348, 1967-1976.
Duan, X., Nauwynck, H.J., Pensaert, M.B., 1997, Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV). Vet Microbiol 56, 9-19.
Dulac, G.C., Afshar, A., 1989, Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can J Vet Res 53, 431-433.
Dungworth, D.L., 1993, In: Pathology of Domestic Animals, Vol 2, 4th Edition. Academic Press, New York, 624-625 pp.
Ellis, J., Clark, E., Haines, D., West, K., Krakowka, S., Kennedy, S., Allan, G.M., 2004, Porcine circovirus-2 and concurrent infections in the field. Vet Microbiol 98, 159-163.
Ellis, J., Krakowka, S., Lairmore, M., Haines, D., Bratanich, A., Clark, E., Allan, G., Konoby, C., Hassard, L., Meehan, B., Martin, K., Harding, J., Kennedy, S., McNeilly, F., 1999, Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J Vet Diagn Invest 11, 3-14.
Ellis, J.A., Bratanich, A., Clark, E.G., Allan, G., Meehan, B., Haines, D.M., Harding, J., West, K.H., Krakowka, S., Konoby, C., Hassard, L., Martin, K., McNeilly, F., 2000, Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12, 21-27.
Fenaux, M., Halbur, P.G., Gill, M., Toth, T.E., Meng, X.J., 2000, Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38, 2494-2503.
Fields, B.N., Knipe, D.M., Howley, P.M., 1996, Orthomyxoviridae, 3rd ed. Edition. Lippincott Raven, Philadelphia, pp1353-1395 pp.
Gall, J.G., Pardue, M.L., 1969, Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A 63, 378-383.
Gilbert, S.A., Larochelle, R., Magar, R., Cho, H.J., Deregt, D., 1997, Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay. J Clin Microbiol 35, 264-267.
Gilpin, D.F., McCullough, B.M., McNeilly, F., McNair, I., Stevenson, L.S., Foster, J.C., Eillis, J.A., Krakowka, S., Adair, B.M., Allan, G.M., 2003, In vitro studies on the infection and replication of porcine xircovirus type 2 in cells of the porcine immune system. Vet Immunol Immunopath 94, 149-161.
Harding, J.C., 1996, Post weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. PROC WEST CAN ASSOC SWINE PRACT: 21.
Harms, P.A., Sorden, S.D., Halbur, P.G., Bolin, S.R., Lager, K.M., Morozov, I., Paul, P.S., 2001, Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38, 528-539.
Hill, B.D., Wright, H.F., 1992, Acute interstitial pneumonia in cattle associated with consumption of mould-damaged sweet potatoes (Ipomoea batatas). Aust Vet J 69, 36-37.
Holtke, H.J., Kessler, C., 1990, Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection. Nucleic Acids Res 18, 5843-5851.
Hsu, S.M., 1990, Immunohistochemistry. Methods Enzymol 184, 357-363.
Hwang, D.M., Chamberlain, D.W., Poutanen, S.M., Low, D.E., Asa, S.L., Butany, J., 2005, Pulmonary pathology of severe acute respiratory syndrome in Toronto. Mod Pathol 18, 1-10.
Jiang, Y., Xu, J., Zhou, C., Wu, Z., Zhong, S., Liu, J., Luo, W., Chen, T., Qin, Q., Deng, P., 2005, Characterization of cytokine/chemokine profiles of severe acute respiratory syndrome. Am J Respir Crit Care Med 171, 850-857.
John, H.A., Birnstiel, M.L., Jones, K.W., 1969, RNA-DNA hybrids at the cytological level. Nature 223, 582-587.
Jung, T., Choi, C., Chae, C., 2002, Localization of swine influenza virus in naturally infected pigs. Vet Pathol 39, 10-16.
Kerr, L.A., Linnabary, R.D., 1989, A review of interstitial pneumonia in cattle. Vet Hum Toxicol 31, 247-254.
Kim, J., Chae, C., 2002, Simultaneous detection of porcine circovirus 2 and porcine parvovirus in naturally and experimentally coinfected pigs by double in situ hybridization. J Vet Diagn Invest 14, 236-240.
Kim, J., Chae, C., 2004, A comparison of virus isolation, polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine circovirus 2 and porcine parvovirus in experimentally and naturally coinfected pigs. J Vet Diagn Invest 16, 45-50.
Kim, J., Choi, C., Chae, C., 2003, Pathogenesis of postweaning multisystemic wasting syndrome reproduced by co-infection with Korean isolates of porcine circovirus 2 and porcine parvovirus. J Comp Pathol 128, 52-59.
Kim, J., Choi, C., Han, D.U., Chae, C., 2001, Simultaneous detection of porcine circovirus type 2 and porcine parvovirus in pigs with PMWS by multiplex PCR. Vet Rec 149, 304-305.
Kim, J., Chung, H.K., Chae, C., 2003, Association of porcine circovirus 2 with porcine respiratory disease complex. Vet J 166, 251-256.
Kim, J., Chung, H.K., Jung, T., Cho, W.S., Choi, C., Chae, C., 2002, Postweaning multisystemic wasting syndrome of pigs in Korea: prevalence, microscopic lesions and coexisting microorganisms. J Vet Med Sci 64, 57-62.
Kim, J., Han, D.U., Choi, C., Chae, C., 2003, Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction. J Vet Med Sci 65, 741-744.
Krakowka, S., Ellis, J.A., Meehan, B., Kennedy, S., McNeilly, F., Allan, G., 2000, Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet Pathol 37, 254-263.
Krakowka, S., Michael-Rings D., Ellis, J.A., Allan, G.M., McCullough, K.C., Botner, A., Nauwynck, H.J., Charreyre, C., 2003, PCV2 disease: intimate relationships between host and pathogen & a close-up on Asia. Merial APVS 1st., 1-70.
Ksiazek, T.G., Erdman, D., Goldsmith, C.S., Zaki, S.R., Peret, T., Emery, S., Tong, S., Urbani, C., Comer, J.A., Lim, W., Rollin, P.E., Dowell, S.F., Ling, A.E., Humphrey, C.D., Shieh, W.J., Guarner, J., Paddock, C.D., Rota, P., Fields, B., DeRisi, J., Yang, J.Y., Cox, N., Hughes, J.M., LeDuc, J.W., Bellini, W.J., Anderson, L.J., 2003, A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 348, 1953-1966.
Kuiken, T., Rimmelzwaan, G.F., Van Amerongen, G., Osterhaus, A.D., 2003, Pathology of human influenza A (H5N1) virus infection in cynomolgus macaques (Macaca fascicularis). Vet Pathol 40, 304-310.
Kurtzman, G., Frickhofen, N., Kimball, J., Jenkins, D.W., Nienhuis, A.W., Young, N.S., 1989, Pure red-cell aplasia of 10 years'' duration due to persistent parvovirus B19 infection and its cure with immunoglobulin therapy. N Engl J Med 321, 519-523.
Larochelle, R., Sauvageau, R., Magar, R., 1994, Immunohistochemical detection of swine influenza virus and porcine reproductive and respiratory syndrome virus in porcine proliferative and necrotizing pneumonia cases from Quebec. Can Vet J 35, 513-515.
Lazarowitz, S.G., Compans, R.W., Choppin, P.W., 1973, Proteolytic cleavage of the hemagglutinin polypeptide of influenza virus. Function of the uncleaved polypeptide HA. Virology 52, 199-212.
Lee, M.S., Chang, P.C., Shien, J.H., Cheng, M.C., Shieh, H.K., 2001, Identification and subtyping of avian influenza viruses by reverse transcription-PCR. J Virol Methods 97, 13-22.
Lee, N., Hui, D., Wu, A., Chan, P., Cameron, P., Joynt, G.M., Ahuja, A., Yung, M.Y., Leung, C.B., To, K.F., Lui, S.F., Szeto, C.C., Chung, S., Sung, J.J., 2003, A major outbreak of severe acute respiratory syndrome in Hong Kong. N Engl J Med 348, 1986-1994.
Liu, Q., Tikoo, S.K., Babiuk, L.A., 2001, Nuclear localization of the ORF2 protein encoded by porcine circovirus type 2. Virology 285, 91-99.
Loneragan, G.H., Gould, D.H., Mason, G.L., Garry, F.B., Yost, G.S., Lanza, D.L., Miles, D.G., Hoffman, B.W., Mills, L.J., 2001, Association of 3-methyleneindolenine, a toxic metabolite of 3-methylindole, with acute interstitial pneumonia in feedlot cattle. Am J Vet Res 62, 1525-1530.
Lopez, A., 1995, Respiratory system. In Thomson’s Special Veterinary Pathology 2nd ed., pp116-174. Edited by W.W. Carlton and M.D. McGavin. Mosby Press, St. Louis, Missouri, USA.
Mankertz, A., Persson, F., Mankertz, J., Blaess, G., Buhk, H.J., 1997, Mapping and characterization of the origin of DNA replication of porcine circovirus. J Virol 71, 2562-2566.
Mardassi, H., Massie, B., Dea, S., 1996, Intracellular synthesis, processing, and transport of proteins encoded by ORFs 5 to 7 of porcine reproductive and respiratory syndrome virus. Virology 221, 98-112.
Mardassi, H., Mounir, S., Dea, S., 1994, Identification of major differences in the nucleocapsid protein genes of a Quebec strain and European strains of porcine reproductive and respiratory syndrome virus. J Gen Virol 75 (Pt 3), 681-685.
Mason, J.N., Tomlinson, I.D., Rosenthal, S.J., Blakely, R.D., 2005, Labeling cell-surface proteins via antibody quantum dot streptavidin conjugates. Methods Mol Biol 303, 35-50.
McGeoch, D., Fellner, P., Newton, C., 1976, Influenza virus genome consists of eight distinct RNA species. Proc Natl Acad Sci U S A 73, 3045-3049.
McNicol, A.M., Farquharson, M.A., 1997, In situ hybridization and its diagnostic applications in pathology. J Pathol 182, 250-261.
Medeiros, R.M., Simoes, S.V., Tabosa, I.M., Nobrega, W.D., Riet-Correa, F., 2001, Bovine atypical interstitial pneumonia associated with the ingestion of damaged sweet potatoes (Ipomoea batatas) in Northeastern Brazil. Vet Hum Toxicol 43, 205-207.
Meehan, B.M., Creelan, J.L., McNulty, M.S., Todd, D., 1997, Sequence of porcine circovirus DNA: affinities with plant circoviruses. J Gen Virol 78 (Pt 1), 221-227.
Meng, X.J., Paul, P.S., Morozov, I., Halbur, P.G., 1996, A nested set of six or seven subgenomic mRNAs is formed in cells infected with different isolates of porcine reproductive and respiratory syndrome virus. J Gen Virol 77 (Pt 6), 1265-1270.
Mengeling, W.L., Cutlip, R.C., 1976, Reproductive disease experimentally induced by exposing pregnant gilts to porcine parvovirus. Am J Vet Res 37, 1393-1400.
Mengeling, W.L., Lager, K.M., Vorwald, A.C., 2000, The effect of porcine parvovirus and porcine reproductive and respiratory syndrome virus on porcine reproductive performance. Anim Reprod Sci 60-61, 199-210.
Mengeling, W.L., Paul, P.S., Brown, T.T., 1980, Transplacental infection and embryonic death following maternal exposure to porcine parvovirus near the time of conception. Arch Virol 65, 55-62.
Meulenberg, J.J., Hulst, M.M., de Meijer, E.J., Moonen, P.L., den Besten, A., de Kluyver, E.P., Wensvoort, G., Moormann, R.J., 1993, Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192, 62-72.
Meulenberg, J.J., Petersen-den Besten, A., 1996, Identification and characterization of a sixth structural protein of Lelystad virus: the glycoprotein GP2 encoded by ORF2 is incorporated in virus particles. Virology 225, 44-51.
Miller, L.C., Laegreid, W.W., Bono, J.L., Chitko-McKown, C.G., Fox, J.M., 2004, Interferon type I response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Arch Virol 149, 2453-2463.
Morin et al., M., Girard, C., Elazhary, Y., ElAzhary, Y., Fajardo, R., Drolet, R., and Lagace, A., 1990, Severe proliferative and necrotizing pneumonia in pigs: a newly recognized disease. Can J Vet 31, 837-839.
Morin et al., M., Phaneuf, J.B., Sauvageau, R., DiFranco, E., Marsolais, G., Boudreault, A., 1981, An epizootic of swine influenza in Quebec. Can Vet J 22, 204-205.
Morozov, I., Sirinarumitr, T., Sorden, S.D., Halbur, P.G., Morgan, M.K., Yoon, K.J., Paul, P.S., 1998, Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 36, 2535-2541.
Murphy, D.S., Hoare, S.F., Going, J.J., Mallon, E.E., George, W.D., Kaye, S.B., Brown, R., Black, D.M., Keith, W.N., 1995, Characterization of extensive genetic alterations in ductal carcinoma in situ by fluorescence in situ hybridization and molecular analysis. J Natl Cancer Inst 87, 1694-1704.
Nauwynck, H.J., Duan, X., Favoreel, H.W., Van Oostveldt, P., Pensaert, M.B., 1999, Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-mediated endocytosis. J Gen Virol 80 (Pt 2), 297-305.
Nicholls, J.M., Poon, L.L., Lee, K.C., Ng, W.F., Lai, S.T., Leung, C.Y., Chu, C.M., Hui, P.K., Mak, K.L., Lim, W., Yan, K.W., Chan, K.H., Tsang, N.C., Guan, Y., Yuen, K.Y., Peiris, J.S., 2003, Lung pathology of fatal severe acute respiratory syndrome. Lancet 361, 1773-1778.
Opriessning, T., Thacker, E.L., Yu, S., Fenaux, M., Meng, X.-J., Halbur, P.G., Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Vet Pathol 41, 624-640.
Paul, P.S., Mengeling, W.L., Brown, T.T., Jr., 1979, Replication of porcine parvovirus in peripheral blood lymphocytes, monocytes, and peritoneal macrophages. Infect Immun 25, 1003-1007.
Pearson, E.G., Andreasen, C.B., Blythe, L.L., Craig, A.M., 1996, Atypical pneumonia associated with ryegrass staggers in calves. J Am Vet Med Assoc 209, 1137-1142.
Pirzadeh, B., Dea, S., 1997, Monoclonal antibodies to the ORF5 product of porcine reproductive and respiratory syndrome virus define linear neutralizing determinants. J Gen Virol 78 (Pt 8), 1867-1873.
Pogranichniy, R.M., Yoon, K.J., Harms, P.A., Sorden, S.D., Daniels, M., 2002, Case-control study on the association of porcine circovirus type 2 and other swine viral pathogens with postweaning multisystemic wasting syndrome. J Vet Diagn Invest 14, 449-456.
Pogranichnyy, R.M., Yoon, K.J., Harms, P.A., Swenson, S.L., Zimmerman, J.J., Sorden, S.D., 2000, Characterization of immune response of young pigs to porcine circovirus type 2 infection. Viral Immunol 13, 143-153.
Pol, J.M., van Dijk, J.E., Wensvoort, G., Terpstra, C., 1991, Pathological, ultrastructural, and immunohistochemical changes caused by Lelystad virus in experimentally induced infections of mystery swine disease (synonym: porcine epidemic abortion and respiratory syndrome (PEARS)). Vet Q 13, 137-143.
Poutanen, S.M., Low, D.E., Henry, B., Finkelstein, S., Rose, D., Green, K., Tellier, R., Draker, R., Adachi, D., Ayers, M., Chan, A.K., Skowronski, D.M., Salit, I., Simor, A.E., Slutsky, A.S., Doyle, P.W., Krajden, M., Petric, M., Brunham, R.C., McGeer, A.J., 2003, Identification of severe acute respiratory syndrome in Canada. N Engl J Med 348, 1995-2005.
Prieto, C., Castro, J.M., 2005, Porcine reproductive and respiratory syndrome virus infection in the boar: a review. Theriogenology 63, 1-16.
Prikhod''ko, G.G., Reyes, H., Vasilyeva, I., Busby, T.F., 2003, Establishment of a porcine parvovirus (PPV) DNA standard and evaluation of a new lightcycler nested-PCR assay for detection of PPV. J Virol Methods 111, 13-19.
Pringle, J.H., Primrose, L., Kind, C.N., Talbot, I.C., Lauder, I., 1989, In situ hybridization demonstration of poly-adenylated RNA sequences in formalin-fixed paraffin sections using a biotinylated oligonucleotide poly d(T) probe. J Pathol 158, 279-286.
Rekik, M.R., Arora, D.J., Dea, S., 1994, Genetic variation in swine influenza virus A isolate associated with proliferative and necrotizing pneumonia in pigs. J Clin Microbiol 32, 515-518.
Rhode, S.L., 3rd, Klaassen, B., 1982, DNA sequence of the 5'' terminus containing the replication origin of parvovirus replicative form DNA. J Virol 41, 990-999.
Roberts, D.H., Cartwright, S.F., Wibberley, G., 1987, Outbreaks of classical swine influenza in pigs in England in 1986. Vet Rec 121, 53-55.
Rosell, C., Segales, J., Domingo, M., 2000, Hepatitis and staging of hepatic damage in pigs naturally infected with porcine circovirus type 2. Vet Pathol 37, 687-692.
Ross, R.F., 1992, Mycoplasma disease. In Diseases of Swine 7th ed., pp537-551. Edited by A.D. Leman, B.E. Straw, W.L. Mengeling, S. D’Alaire, and D.J. Taylor. Iowa State University Press, Ames, Iowa, USA.
Saoulidis, K., Kyriakis, S.C., Kennedy, S., Lekkas, S., Miliotis Ch, C., Allan, G., Balkamos, G.C., Papoutsis, P.A., 2002, First report of post-weaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome in pigs in Greece. J Vet Med B Infect Dis Vet Public Health 49, 202-205.
Segales, J., Calsamiglia, M., Rosell, C., Soler, M., Maldonado, J., Martin, M., Domingo, M., 2002, Porcine reproductive and respiratory syndrome virus (PRRSV) infection status in pigs naturally affected with post-weaning multisystemic wasting syndrome (PMWS) in Spain. Vet Microbiol 85, 23-30.
Segales, J., Rosell, C., Domingo, M., 2004, Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. Vet Microbiol 98, 137-149.
Shimizu, M., Yamada, S., Murakami, Y., Morozumi, T., Kobayashi, H., Mitani, K., Ito, N., Kubo, M., Kimura, K., Kobayashi, M., et al., 1994, Isolation of porcine reproductive and respiratory syndrome (PRRS) virus from Heko-Heko disease of pigs. J Vet Med Sci 56, 389-391.
Sirinarumitr, T., Zhang, Y., Kluge, J.P., Halbur, P.G., Paul, P.S., 1998, A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo. J Gen Virol 79 (Pt 12), 2989-2995.
Stevenson, G.W., Kiupel, M., Mittal, S.K., Choi, J., Latimer, K.S., Kanitz, C.L., 2001, Tissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors. J Vet Diagn Invest 13, 57-62.
Suarez, P., Diaz-Guerra, M., Prieto, C., Esteban, M., Castro, J.M., Nieto, A., Ortin, J., 1996, Open reading frame 5 of porcine reproductive and respiratory syndrome virus as a cause of virus-induced apoptosis. J Virol 70, 2876-2882.
Sweet, C., Smith, H., 1980, Pathogenicity of influenza virus. Microbiol Rev 44, 303-330.
Tattersall, P. and Cotmore, S.F., 1990. Reproduction of autonomous parvovirus DNA. In: Tijssen, P., Editor, , 1990. Handbook of Parvoviruses 1, CRC Press, Boca Raton.
Thacker, E.L., 2001, Porcine respiratory disease complex - what is it and why does it remain a problem? Pig J. 48, 66-70.
Thacker, E.L., Halbur, P.G., Ross, R.F., Thanawongnuwech, R., Thacker, B.J., 1999, Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. J Clin Microbiol 37, 620-627.
Thacker, E.L., Thacker, B.J., Janke, B.H., 2001, Interaction between Mycoplasma hyopneumoniae and swine influenza virus. J Clin Microbiol 39, 2525-2530.
Thanawongnuwech, R., Halbur, P.G., Ackermann, M.R., Thacker, E.L., Royer, R.L., 1998, Effects of low (modified-live virus vaccine) and high (VR-2385)-virulence strains of porcine reproductive and respiratory syndrome virus on pulmonary clearance of copper particles in pigs. Vet Pathol 35, 398-406.
Thomson, J., Smith, B., Allan, G., McNeilly, F., McVicar, C., 2000, PDNS, PMWS and porcine circovirus type 2 in Scotland. Porcine dermatitis and nephropathy syndrome. Post-weaning multisystemic wasting syndrome. Vet Rec 146, 651-652.
Tischer, I., Gelderblom, H., Vettermann, W., Koch, M.A., 1982, A very small porcine virus with circular single-stranded DNA. Nature 295, 64-66.
Tischer, I., Peters, D., Rasch, R., Pociuli, S., 1987, Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch Virol 96, 39-57.
Tsai, C.P., Pan, M.J., 2003, New H1N2 and H3N1 influenza viruses in Taiwanese pig herds. Vet Rec 153, 408.
Udobi, K.F., Childs, E.D., Touijer, K., 2003, Acute Respiratory Distress Syndrome. American Family Physician 67, 315-322.
Van Alstine, W.G., Kanitz, C.L., Stevenson, G.W., 1993, Time and temperature survivability of PRRS virus in serum and tissues. J Vet Diagn Invest 5, 621-622.
Voicu, I.L., Silim, A., Morin et al., M., Elazhary, M.A., 1994, Interaction of porcine reproductive and respiratory syndrome virus with swine monocytes. Vet Rec 134, 422-423.
Wang, C., Huang, T.S., Huang, C.C., Tu, C., Jong, M.H., Lin, S.Y., Lai, S.S., 2004, Characterization of porcine circovirus type 2 in Taiwan. J Vet Med Sci 66, 469-475.
White, J., Matlin, K., Helenius, A., 1981, Cell fusion by Semliki Forest, influenza, and vesicular stomatitis viruses. J Cell Biol 89, 674-679.
Wong, C.K., Lam, C.W., Wu, A.K., Ip, W.K., Lee, N.L., Chan, I.H., Lit, L.C., Hui, D.S., Chan, M.H., Chung, S.S., Sung, J.J., 2004, Plasma inflammatory cytokines and chemokines in severe acute respiratory syndrome. Clin Exp Immunol 136, 95-103.
Yoon, I.J., Joo, H.S., Christianson, W.T., Kim, H.S., Collins, J.E., Morrison, R.B., Dial, G.D., 1992, An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J Vet Diagn Invest 4, 144-147.
Yoon, K.J., Zimmerman, J.J., Swenson, S.L., McGinley, M.J., Eernisse, K.A., Brevik, A., Rhinehart, L.L., Frey, M.L., Hill, H.T., Platt, K.B., 1995, Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection. J Vet Diagn Invest 7, 305-312.
Young, B., Heath, J.W., 2000, Wheater''s functional histology., 4th Edition. CHURCHILL LIVINGSTONE, London, 222-236 pp.
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