神經退化性疾病在全世界發生的比例相當高，，且大部分為家族性遺傳，主要症狀包括認知功能的衰退以及記憶力的逐漸喪失，原因為神經記憶細胞接受不正常的訊息傳遞而產生細胞凋亡。而如果能夠預測細胞凋亡訊息傳遞的路徑，便能提早抑制病情的惡化，讓醫學治療有更進一步的發展。
首先我們先建立細胞凋亡訊息蛋白質相關詞彙字庫（SWISS-PROT），再從線上生物醫學文獻（PubMed）下載神經退化性疾病細胞凋亡相關文獻摘要，並且搜尋線上蛋白質資料庫（KEGG）中神經退化性疾病之細胞凋亡訊息蛋白質傳遞路徑，接著利用文獻探勘技術，使用已建立之細胞凋亡訊息蛋白質相關詞彙字庫，以程式自動在生物醫學文獻摘要中搜尋神經退化性疾病細胞凋亡相關蛋白質分別在，（1）一個句子、（2）二個句子、（3）一篇文獻，中同時出現的兩兩蛋白質關係，再與已知線上神經退化性疾病細胞凋亡訊息傳遞路徑資料庫的蛋白質兩兩關係做比較，找出兩者重複建立的部分，把這個結果與線上已知之細胞凋亡訊息傳遞路徑資料庫蛋白質關係作比較，由此找出尚未建立之細胞凋亡訊息傳遞路徑圖。

關鍵字：神經退化性疾病、細胞凋亡、訊息傳遞

Neurodegenerative disorders get higher quite in the proportions to be taken place at whole worlds and whether majority for family heredity, the main symptom includes the decline of the cognitive function and gradual forfeiture of memory, the reason is accepted by neural memory cell the abnormal signal transduction produces apoptosis . And if can predict that the apoptosis in the signal transduction pathway , can suppress the deterioration of the condition ahead of time , let medical science be treated and got further development.
First we first establish the apoptosis of related protein glossary database (SWISS-PROT), then downloads the neurodegenerative disorders of apoptosis related abstract from the on-line biomedicine literature (PubMed) , and searches
for the protein information database (KEGG) of the neurodegenerative disorders of apoptosis related pathway, then uses the data mining technology,with apoptosis of protein related glossary database use already establishment. Automatically searches for the neurodegenerative disorders of apoptosis related protein by the program in the biomedicine literature abstract separately in, (1) a sentence, (2) two sentences, (3) a
literature, finding simultaneously appears protein-protein interation. And comparing to the neurodegenerative disorders of apoptosis related protein database, looking for their repetition. This result comparing to the known neurodegenerative disorders of apoptosis related pathway, thus looking for unestablished neurodegenerative disorders of apoptosis pathway.

Key word: Neurodegenerative disorder, Apoptosis, Signal transduction

文件目錄
中文摘要………………………………………….………………..……..i
英文摘要………………………………………….………………..…….ii
誌謝………………………………………….………………..…………iii
文件目錄…………………………………….………………..…………iv
圖目錄……………………………………….……………….…………vii
表目錄……………………………………….………………..…………ix
縮寫字對照表……………………………….………………..……….…x

第一章 序論………………………………………….…………………1
1.1 研究背景……………………………………………………….………..1
1.2 研究目的…………………………………………………………….…..2
1.3 研究架構……………………………………………………………..….2
1.4 論文架構……………………………………………………………..….5
第二章 文獻回顧……………………………………………………….6
2.1 神經退化性疾病…………………………………………………….…..6
2.1.1阿茲海默症…………………………………………………...6
2.1.2帕金森氏症…………………………………………………...6
2.1.3亨丁頓舞蹈症…………………………………………….…..7
2.2 訊息傳遞……………………………………………………………….8
2.3 細胞凋亡……………………………………………………………….9
第三章 相關線上資料庫簡介………………………………..………..10
3.1 NCBI…………………………………………………………….…….10
3.1.1 PubMed………………………………………………………11
3.2 KEGG…………………………………………………………...……..12
3.3 SWISS-PROT……………………………………………………….....13
第四章 研究方法………………………………………………..……..15
4.1利用文獻探勘技術分析生物醫學文獻建立蛋白質線性關係…...….15
4.2利用文獻探勘技術分析生物醫學文獻建立蛋白質線性關係………15
4.2.1國際期刊研究文獻摘要資料蒐集…………………………...15
4.2.2 相關蛋白質字庫建立………………………………..………16
4.2.3 尋找文獻的規則性…………………………………………..17
4.2.4 程式開發:在相關文獻摘要中出現兩次以上的蛋白質關係18
4.3 利用人工方式建立已知的訊息蛋白質傳遞路徑兩兩相關蛋白質關係
……….……………………………………………………………………………….19
4.3.1資料來源…………………………………..………………….19
4.3.2方法與步驟………………………………..………………….21
第五章 結果……….…………………………………………………...22
5.1 細胞凋亡訊息蛋白質相關詞彙字庫…………………………………22
5.2在相關文獻摘要中出現兩次以上的蛋白質關係……………….……23
5.3 利用人工方式建立已知的訊息蛋白質傳遞路徑兩兩相關蛋白質關係
……….……………………………………………………………………………….24
5.4 比較關係………………………………………………………………25
第六章 綜合討論……………………………………………....………28
6.1 討論一……………………………………………………...…………28
6.2 討論二……………………………………………………...…………28
6.3討論三…………………….………………………………...…………28
6.4討論四……………………………………………………...….………29
參考文獻…………………………………..……………………………30
附錄1 細胞凋亡訊息蛋白質相關詞彙字庫共710個……………….33
附錄2 程式碼…………………………..………………………………50
附錄3 Publication………………………..…………………………..…57

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