(54.236.58.220) 您好!臺灣時間:2021/03/04 22:42
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:劉哲亨
研究生(外文):Che-Heng Liu 
論文名稱:以嵌段式聚合微膠體投與大分子和小分子物質到裸鼠的不同組織
論文名稱(外文):Delivery of Large and Small Molecules to Different Tissues of Nude Mice by Polymeric Micelles (PM)
指導教授:廖嘉鴻
指導教授(外文):Jiahorng Liaw
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:藥學系
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
中文關鍵詞:微膠體裸鼠藥物
外文關鍵詞:Polymeric Micellesgene deliveryMethylprednisolone
相關次數:
  • 被引用被引用:0
  • 點閱點閱:101
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:10
  • 收藏至我的研究室書目清單書目收藏:0
本研究的初步目標,在於以嵌段式聚合微膠體作為載體,包覆三種物質,第一種是具有非特異性啟動子質體:pCMV-LacZ,以每天投與三個口服劑量,一共兩天的方式,在口服投與六個劑量,四十八小時之後,其基因的表現可以在裸鼠脊髓組織經由X-gal定性染色的方式觀察得到。第二種是具有特異性啟動子質體:pKeratin 12-LacZ,以每天點眼液投與三個劑量,一共兩天的方式,在投與六個劑量,四十八小時之後,而其基因的表現可以在裸鼠眼睛的上皮組織組織有所表現。第三種是醣質類固醇藥物-methylprednisolone (MP), 以靜脈注射的方式投與,觀察在七小時之內signal transducers and activators of transcription 5a (STAT5a), growth associated protein-43 (GAP-43) 基因表現,結果可以觀察到在裸鼠脊髓組織內STAT5a和GAP43有被MP影響其效果。
The goal of this project was used polymeric micelles (PM) as a molecule carrier. Three types of molecules were selected. First, orally deliver a non-specificity promoter plasmid (pCMV-LacZ) (7.2Kb). There times a day with six doses, after 48 hours, the expression of LacZ gene was observed in the nude mouse spinal cord tissue by the X-gal qualitative measurement. Secondly, eye drops deliver a specificity cornea-epithelial promoter plasmid (pKeratin 12-LacZ) (7.4Kb). There times a day with six doses, after 48 hours, LacZ the gene was also observed in the nude mouse epithelial region tissue. Thirdly, intravenous delivery of methylprednisolone (MP) was observed the signal transducers and activators of transcription 5a (STAT5a) and growth associated protein-43 (GAP-43) gene expression with 7 hours. It was found that STAT5a and GAP-43 were modified by PM in the nude mouse spinal cord tissue.
目錄
目錄---------------------------------------------------------------------------------I
附圖目錄-------------------------------------------------------------------------VI
中文摘要-----------------------------------------------------------------------VIII
英文摘要-------------------------------------------------------------------------IX
第一章 序論-----------------------------------------------------------------------1
第一節 嵌段式共聚合物--------------------------------------------------------1
第二節 基因治療與方法--------------------------------------------------------1
1.基因治療-------------------------------------------------------------------------1
2.基因載體-------------------------------------------------------------------------1
2.1病毒型載體------------------------------------------------------------------2
2.1.1反轉錄病毒載體-------------------------------------------------------2
2.1.2 Lentivirus ---------------------------------------------------------------2
2.1.3腺病毒載體------------------------------------------------------------3
2.1.4腺相關病毒載體-------------------------------------------------------3
2.2非病毒型載體---------------------------------------------------------------4
2.2.1直接注射----------------------------------------------------------------4
2.2.2微脂粒-------------------------------------------------------------------4
2.2.3高分子聚合物----------------------------------------------------------5
2.2.4物理方法---------------------------------------------------------------6
第三節 細胞專一性的基因傳遞----------------------------------------------6
1.投與路徑的選擇-------------------------------------------------------------6
2.載體或是配位基-------------------------------------------------------------7
3.組織或細胞專一性的啟動子----------------------------------------------7
4.專一性啟動因子-Keratins(角蛋白)-------------------------------------7
5.眼睛中角膜的構造----------------------------------------------------------7
第四節 醣質類固醇藥與訊息傳遞 ------------------------------------------9
1.醣質類固醇藥物-------------------------------------------------------------9
2.信號傳導和轉錄啓動因數5a --------------------------------------------9
3.生長相關的蛋白質43-----------------------------------------------------11
第二章 研究目的---------------------------------------------------------------12
第三章 研究方法與步驟------------------------------------------------------13
第一節 質體 DNA---------------------------------------------------------------13
1.質體 DNA的種類---------------------------------------------------------13
2.質體DNA之大量製備----------------------------------------------------13
第二節 聚合微膠體溶液------------------------------------------------------14
1.聚合微膠體之形成(臨界微膠濃度)------------------------------------14
2.聚合微膠體溶液之製備--------------------------------------------------14
3.聚合微膠體溶液之物化性質---------------------------------------------15
聚合微膠體溶液之表面電位(zeta potential)與粒徑大小----------15
4.DNA-聚合微膠體溶液之安定性----------------------------------------15
4.1聚合微膠體口服液----------------------------------------------------15
4.2聚合微膠體點眼液----------------------------------------------------15
第三節 體內基因傳遞藥物的傳遞------------------------------------------16
1.裸鼠(BALB/c-nu)------------------------------------------------------16
第四節 蛋白質濃度之測定---------------------------------------------------16
1.標準曲線的配製-----------------------------------------------------------16
2.檢品分析流程--------------------------------------------------------------17
第五節 b-galactosidase 的定量分析----------------------------------------17
1.標準曲線的配製-----------------------------------------------------------17
2.檢品分析流程--------------------------------------------------------------18
第六節 b-galactosidase之定性分析-----------------------------------------18
1.實驗流程--------------------------------------------------------------------19
第七節 反轉錄-聚合酵素鏈鎖反應-----------------------------------------19
1.組織RNA之萃取----------------------------------------------------------19
2.第一股cDNA的製備------------------------------------------------------21
2.1 b-actin的check-------------------------------------------------------21
2.2 b-gal--------------------------------------------------------------------22
第八節 膠體電泳位移分析---------------------------------------------------23
1.細胞核蛋白的製備--------------------------------------------------------23
2.DNA探針的序列-----------------------------------------------------------23
3.探針的標定-----------------------------------------------------------------24
4.膠體電泳位移分析--------------------------------------------------------24
第九節 即時聚合酶連鎖反應法---------------------------------------------25
1. SYBR-system -------------------------------------------------------------25
1.1實驗流程---------------------------------------------------------------25
2. TaqMan system------------------------------------------------------------26
2.1實驗流程 -------------------------------------------------------------27
附圖--------------------------------------------------------------------------------28
第四章 研究成果與討論------------------------------------------------------33
第一節 聚合微膠體溶液之表面電位大小---------------------------------33
1.聚合微膠體口服液--------------------------------------------------------33
2.聚合微膠體點眼液--------------------------------------------------------33
3.聚合微膠體注射液--------------------------------------------------------33
第二節 聚合微膠體溶液之粒徑大小---------------------------------------34
1.聚合微膠體口服液--------------------------------------------------------34
2.聚合微膠體點眼液--------------------------------------------------------34
3.聚合微膠體注射液--------------------------------------------------------34
第三節 臨界微膠濃度的測定------------------------------------------------35
第四節 DNase I安定性分析--------------------------------------------------35
1.聚合微膠體口服液--------------------------------------------------------35
2.聚合微膠體點眼液--------------------------------------------------------35
第五節 b-galactosidase 的定量分析----------------------------------------36
1.裸鼠脊隨組織b-galactosidase 的定量分析---------------------------36
2.裸鼠眼球組織b-galactosidase 的定量分析---------------------------36
第六節 b-galactosidase之定性分析-----------------------------------------37
1裸鼠脊隨組織b-galactosidase 的定性分析----------------------------37
第七節 膠體電泳位移分析---------------------------------------------------37
第八節 即時聚合酶連鎖反應法之分析(real time Polymerase Chain Reaction) --------------------------------------------------------------38
第五章 結論---------------------------------------------------------------------39
附圖--------------------------------------------------------------------------------41
第六章 參考文獻---------------------------------------------------------------50
第六章 參考文獻
1.Otsuka H, Nagasaki Y, Kataoka K. PEGylated nanoparticles for biological and pharmaceutical applications. Adv Drug Deliv Rev. 2003; 55: 403-419.
2.Adams ML, Lavasanifar A, Kwon GS. Amphiphilic block copolymers for drug delivery. J Pharm Sci. 2003; 92: 1343-1355.
3.Aiuti A, Ficara F, Cattaneo F, Bordignon C, Roncarolo MG. Gene therapy for adenosine deaminase deficiency. Curr Opin Allergy Clin Immunol. 2003; 3: 461-466.
4.Hirschhorn, R. Adenosine deaminase deficiency: molecular basis and recent developments. Clin. Immunol. Immunopathol 1995; 76: S219-S227.
5.Apasov SG, Blackburn MR, Kellems RE, Smith PT, Sitkovsky MV. Adenosine deaminase deficiency increases thymic apoptosis and causes defective T cell receptor signaling. J. Clin. Invest. 2001; 108: 131-141.
6.Nelson AW, James MW. Methods of gene delivery. Gene Ther.1998; 12: 483-501.
7.Niidome T, Huang L. Gene therapy progress and prospects: nonviral vectors. Gene Ther. 2002; 9: 1647-1652.
8.Ronald GC. Transfer of genes to humans: early lessons and obstacles to success. Science. 1995; 270: 404-410.
9.Lever AM, Strappe PM, Zhao J. Lentiviral vectors. J Biomed Sci. 2004; 11: 439-449.
10.Shiraishi M, Nagahama M, Obuchi Y, Taira K, Tomori H, Sugawa H, Kusano T, Muto Y. Successful gene transfer to the porcine liver in vivo with an adenoviral vector. J Surg Res. 1998; 76: 105-110.
11.Kotin RM, Siniscalco M, Samulski RJ, Zhu XD, Hunter L, Laughlin CA, McLaughlin S, Muzyczka N, Rocchi M, Berns KI. Site-spectific integration by adeno-associated virus.Proc.Natl Acad.Sci. USA. 1990; 87: 2211-2215.
12.Dickson G, Roberts ML, Wells DJ, Fabb SA. Recombinant micro-genes and dystrophin viral vectors. Neuromuscul Disord. 2002; 12: S40-44.
13.Wolff JA, Malone RW, Williams P, Chong W, Acsadi G, Jani A, Felgener, PL. Direct gene transfer into mouse muscle in vivo. Science. 1990; 247: 1465-1468.
14.Kawabata K, Takakura Y, Hashida M. The fate of plasma DNA after intravenous injection in mice: involvement of scavenger receptors in its hepatic uptake. Pharm. res. 1995; 12: 825-830.
15.Mahato RI, Kawabata K, Takakura Y, Hashida M. In vivo disposition characteristic of plasmid DNA complexed with cationic liposomes. J. Drug Target. 1995; 3: 149-157.
16.Tranchant I, Thompson B, Nicolazzi C, Mignet N, Scherman D. Physicochemical optimisation of plasmid delivery by cationic lipids. J Gene Med. 2004; 6: S24-35.
17.Hyndman L, Lemoine JL, Huang L, Porteous DJ, Boyd AC, Nan X. HIV-1 Tat protein transduction domain peptide facilitates gene transfer in combination with cationic liposomes. J Control Release. 2004; 99: 435-444.
18.Lee JT, Watarai S, Kakidani H, Onuma M, Zhao DD, Yasuda T. Evaluation of cationic liposomes for delivery of diphtheria toxin A-chain gene to cells infected with bovine leukemia virus. J Vet Med Sci. 1997; 59: 169-174.
19.Song LY, Ahkong QF, Rong Q, Wang Z, Ansell S, Hope MJ, Mui B. Characterization of the inhibitory effect of PEG-lipid conjugates on the intracellular delivery of plasmid and antisense DNA mediated by cationic lipid liposomes. Biochim Biophys Acta. 2002; 1558: 1-13.
20.Riche EL, Erickson BW, Cho MJ. Novel long-circulating liposomes containing peptide library-lipid conjugates: synthesis and in vivo behavior. J Drug Target. 2004; 12: 355-361.
21.Shi N, Pardridge WM. Noninvasive gene targeting to the brain. Proc Natl Acad Sci U S A. 2000; 97: 7567-7572.
22.Hacker U, Albrecht R, Maniak M. Fluid-phase uptake by macropinocytosis in Dictyostelium. J Cell Sci. 1997; 110: 105-112.
23.Gomez-Valades AG, Molas M, Vidal-Alabro A, Bermudez J, Bartrons R, Perales JC. Copolymers of poly-L-lysine with serine and tryptophan form stable DNA vectors: implications for receptor-mediated gene transfer. J Control Release. 2005; 102: 277-291.
24.Godbey WT, Barry MA, Saggau P, Wu KK, Mikos AG. Poly (ethylenimine)-mediated transfection: a new paradigm for gene delivery. J Biomed Mater Res. 2000; 51: 321-328.
25.Chen J, Yang WL, Li G, Qian J, Xue JL, Fu SK, Lu DR. Transfection of mEpo gene to intestinal epithelium in vivo mediated by oral delivery of chitosan-DNA nanoparticles. World J Gastroenterol. 2004; 10: 112-116.
26.Roy K, Mao HQ, Huang SK, Leong KW. Oral gene delivery with chitosan--DNA nanoparticles generates immunologic protection in a murine model of peanut allergy. Nat Med. 1999; 5: 387-391.
27.Chang SF, Chang HY, Tong YC, Chen SH, Hsaio FC, Lu SC, Liaw J. Nonionic polymeric micelles for oral gene delivery in vivo. Hum Gene Ther. 2004; 15: 481-493.
28.Liu F, Hunag L. Electric gene transfer to the liver following systemic dministration of plasmid DNA. Gene Ther. 1998; 8: 1531–1537.
29.Lin MT, Pulkkinen L, Uitto J, Yoon K. The gene gun: current application in cutaneous gene therapy. Int J Dermatol 2000; 39: 161–170.
30.Davidson JM, Krieg T, Eming SA. Particle-mediated gene therapy of wounds. Wound Repair Regen. 2000; 8: 452–459.
31.Muangmoonchai R, Wong SC, Smirlis D, Phillips IR, Shephardl EA. Transfection of liver in vivo by biolistic particle delivery: its use in the investigation of cytochrome P450 gene regulation. Mol Biotechnol 2002; 20: 145–151.
32.Kuriyama S, Mitoro A, Tsujinoue H, Nakatani T, Yoshiji H, Tsujimoto T, Yamazaki M, Fukui H. Particle-mediated gene transfer into murine livers using a newly developed gene gun. Gene Ther 2000; 7: 1132–1136.
33.Yoshida S, Kashiwamura SI, Hosoya Y, Luo E, Matsuoka H, Ishii A, Fujimura A, Kobayashi E. Direct immunization of malaria DNA vaccine into the liver by gene gun protects against lethal challenge of Plasmodium berghei sporozoite. Biochem Biophys Res Commun 2000; 271: 107–115.
34.Amabile PG, Waugh JM, Lewis TN, Elkins CJ, Janas W, Dake MD.High-efficiency endovascular gene delivery via therapeutic ultrasound. J Am Coll Cardiol 2001; 37: 1975–1980.
35.Taniyama Y, Tachibana K, Hiraoka K, Namba T, Yamasaki K, Hashiya N, Aoki M, Ogihara T, Yasufumi K, Morishita R.Local delivery of plasmid DNA into rat carotid artery using ultrasound. Circulation 2002; 105: 1233–1239.
36.Gao Z, Fain HD, Rapoport N. Ultrasound-enhanced tumor targeting of polymeric micellar drug carriers. Mol Pharm. 2004; 1: 317-330.
37.Unger EC, Hersh E, Vannan M, McCreery T. Gene delivery using ultrasound contrast agents. Echocardiography 2001; 18: 355– 361.
38.Lawrie A, Brisken AF, Francis SE, Cumberland DC, Crossman DC, Newman CM.Microbubble-enhanced ultrasound for vascular gene delivery. Gene Ther 2000; 7: 2023–2027.
39.Song J, Chappell JC, Qi M, VanGieson EJ, Kaul S, Price RJ. Influence of injection site, microvascular pressure and ultrasound variables on microbubble-mediated delivery of microspheres to muscle. J Am Coll Cardiol 2002; 39: 726–731.
40.Shohet RV, Chen S, Zhou YT, Wang Z, Meidell RS, Unger RH, Grayburn PA. Echocardiographic destruction of albumin microbubbles directs gene delivery to the myocardium. Circulation 2000; 101: 2554–2556.
41.Tousignant JD, Gates AL, Ingram LA, Johnson CL, Nietupski JB, Cheng SH, Eastman SJ, Scheule RK. Comprehensive analysis of the acute toxicities induced by systemic administration of cationic lipid: plasmid DNA complexes in mice. Hum Gene Ther 2000; 11: 2493–2513.
42.Unger EC, Hersh E, Vannan M, Matsunaga TO, McCreery T. Local drug and gene delivery through microbubbles. Prog Cardiovasc Dis. 2001; 44: 45-54.
43.Mastrobattista E, Kapel RH, Eggenhuisen MH, Roholl PJ, Crommelin DJ, Hennink WE, Storm G. Lipid-coated polyplexes for targeted gene delivery to ovarian carcinoma cells. Cancer Gene Ther. 2001; 8: 405-413.
44.Beusechem VW, Grill J, Mastenbroek DC, Wickham TJ, Roelvink PW, Haisma HJ, Lamfers ML, Dirven CM, Pinedo HM, Gerritsen WR. Efficient and selective gene transfer into primary human brain tumors by using single-chain antibody-targeted adenoviral vectors with native tropism abolished. J Virol. 2002; 76: 2753-2762.
45.Pollmann A, Kabisch H, Block A, Muller J, Hellwinkel OJ. Limited specificity of promoter constructs for gene therapy in osteosarcoma. Int J Mol Med. 2004; 14: 737-742.
46.Boccaccio GL, Colman DR. Myelin basic protein mRNA localization and polypeptide targeting. J Neurosci Res. 1995; 42: 277-286.
47.Nishida K, Honma Y, Dota A, Kawasaki S, Adachi W, Nakamura T, Quantock AJ, Hosotani H, Yamamoto S, Okada M, Shimomura Y, Kinoshita S. Isolation and chromosomal localization of a cornea-specific human keratin 12 gene and detection of four mutations in Meesmann corneal epithelial dystrophy. Am J Hum Genet. 1997; 61: 1268-1275.
48.Liu JJ, Kao WW, Wilson SE. Corneal epithelium-specific mouse keratin K12 promoter. Exp Eye Res. 1999; 68: 295-301.
49.Kinoshita S, Adachi W, Sotozono C, Nishida K, Yokoi N, Quantock AJ, Okubo K. Characteristics of the human ocular surface epithelium. Prog Retin Eye Res. 2001; 20: 639-673.
50.Ladage PM, Yamamoto K, Ren DH, Jester JV, Petroll WM, Bergmanson JP, Cavanagh HD. Recovery time of corneal epithelial proliferation in the rabbit following rigid gas-permeable extended contact-lens wear. Eye Contact Lens. 2003; 29: 61-64.
51.Ducker TB.Hamit HF. Experimental treatment of acute spinal cord injury J Neurosurg 1969; 30: 693-7.
52.Black P, Markowitz RS. Experimental spinal cord injury in monkeys:Comparison off steroids and local hypothermia Surg Forum 1971; 22: 409-411.
53.Green BA, Kahn T, Klose KJ. A comparative study of steroid therapy in acute experimental spinal cord injury. Surg Neurol. 1980; 13: 91-7.
54.Hall ED. Yonkers PA. Biochemistry and pharmacology of lipid antioxidants in acute brain and spinal cord injury. J of Neurotrauma.1992; 9: S425-442.
55.Cai M, Ogawa R. Effects of free radical scavengers, methylprednisolone, and ulinastatin on acute xanthine and xanthine oxidase-induced lung injury in rats. Circ Shock. 1994; 43: 71-8.
56.Ray SK, Wilford GG, Matzelle DC, Hogan EL, Banik NL. Calpeptin and methylprednisolone inhibit apoptosis in rat spinal cord injury. Ann N Y Acad Sci. 1999; 890: 261-269.
57.Sadrzadeh SM, Anderson DK, Panter SS, Hallaway PE, Eaton JW. Hemoglobin potentiates central nervous system damage. J Clin Invest. 1987; 79: 662-664.
58.Dumon S, Santos SC, Debierre-Grockiego F, Gouilleux-Gruart V, Cocault L, Boucheron C, Mollat P, Gisselbrecht S, Gouilleux F. IL-3 dependent regulation of Bcl-xL gene expression by STAT5 in a bone marrow derived cell line. Oncogene. 1999; 18: 4191-4199.
59.Sevilla L, Zaldumbide A, Carlotti F, Dayem MA, Pognonec P, Boulukos KE. Bcl-xL expression correlates with primary macrophage differentiation, activation of functional competence, and survival and results from synergistic transcriptional activation by Ets2 and PU.1. J Biol Chem. 2001; 276: 17800-17807.
60.Kim DH, Jahng TA. Continuous brain-derived neurotrophic factor (BDNF) infusion after methylprednisolone treatment in severe spinal cord injury. J Korean Med Sci. 2004; 19: 113-122.
61.Akira S. Functional roles of STAT family proteins: lessons from knockout mice. Stem Cells. 1999; 17: 138-146.
62.Cella N, Groner B, Hynes NE. Characterization of Stat5a and Stat5b homodimers and heterodimers and their association with the glucocortiocoid receptor in mammary cells. Mol Cell Biol. 1998; 18: 1783-1792.
63.Calo V, Migliavacca M, Bazan V, Macaluso M, Buscemi M, Gebbia N, Russo A. STAT proteins: from normal control of cellular events to tumorigenesis. J Cell Physiol. 2003; 197: 157-168.
64.Darnell JE. STATs and gene regulation. Science.1997; 277: 1630-1635.
65.Bulsara KR, Iskandar BJ, Villavicencio AT, Skene JH. A new millenium for spinal cord regeneration: growth-associated genes. Spine. 2002; 27: 1946-1949.
66.Ohlsson M, Westerlund U, Langmoen IA, Svensson M. Methylprednisolone treatment does not influence axonal regeneration or degeneration following optic nerve injury in the adult rat. J Neuroophthalmol. 2004; 24: 11-8.
67.Orchinik M, Carroll SS, Li YH, McEwen BS, Weiland, NG. Heterogeneity of hippocampal GABA (A) receptors: regulation by corticosterone. J. Neurosci. 2001; 21: 330-339.
68.Ariyoshi M, Akasu T. Glucocorticoid modulates the sensitivity of the GABA (A) receptor on primary afferent neurons of bullfrogs. Brain Res.1986; 367: 332-336.
69.Fukura H, Kitani Y, Komiya Y, Igarashi M. GABA (A) receptor in growth cones: the outline of GABA (A) receptor-dependent signaling in growth cones is applicable to a variety of alpha-subunit species. J Neurosci Res. 1999; 58: 407-416
70.Liaw J, Chang SF, Hsiao FC. In vivo gene delivery into ocular tissues by eye drops of poly (ethylene oxide)-poly (propylene oxide)-poly (ethylene oxide) (PEO-PPO-PEO) polymeric micelles. Gene Ther. 2001; 8: 999-1004.
71.Weihong L, David A. Validation of a quantitative method for real time PCR kinetics. Biochemical and Biophysical Research Communications 2002; 294: 347–353.
72.Groner B. Transcription factor regulation in mammary epithelial cells. Domest Anim Endocrinol. 2002; 23: 25-32.
73.Buettner R, Mora LB, Jove R. Activated STAT signaling in human tumors provides novel molecular targets for therapeutic intervention. Clin Cancer Res. 2002; 8: 945-954.
74.http://probes.invitrogen.com/handbook/images/g001355.gif
75.http://www.ukl.uni-freiburg.de/core-facility/taqman/taqman_img/taqmn.gif
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 王家通(民85)。教師法與教師專業。教師天地,83期,22~26。
2. 吳煥烘(民85)。從學校組織架構論教師專業精神。教師之友,37卷1期,11~16。
3. 呂錘卿、林生傳(民90)。國民小學教師專業成長指標及現況之研究。國立高雄師範大學教育學系教育學刊,17期,45~64。
4. 李安明(民87)。我國國小校長教學領導之研究與省思。教育研究資訊,6卷6期,121~146。
5. 沈姍姍(民84)。教師法未必能提昇教師的專業地位。教育心,10期,22~23。
6. 沈姍姍(民85)。專業主義、教師權力與教育行政體制關係:教師權力消長的動態研究。新竹師院學報,9期,103~128。
7. 沈姍姍(民87)。學校教育改革中教師的角色調整。教育資料與研究,22期,8~9。
8. 沈姍姍(民89)。教師專業成長的誘因與動力—自制度面分析。學校行政雙月刊,9期,28~31。
9. 姜添輝(民89)。論教師專業意識、社會控制與保守文化。教育與社會研究,1期,1~24。
10. 馬信行(民85)。從社會學角度析論教育專業—下波師範教育改革方向的構想。教師天地,83期,18~21。
11. 張德銳、李俊達(民88)。以學校為中心的教師專業成長。研習資訊,16卷6期,25~31。
12. 陳美玉(民85)。結構性限制與教師專業發展。教育研究雙月刊,52期,53~62。
13. 劉春榮(民85b)。教師專業自主權與學生受教權之關聯。教育資料與研究,10期,42~43。
14. 劉春榮(民87a)。教師專業自主。教育資料集刊,23輯,25~38。
15. 劉春榮(民87b)。教師組織與教師專業成長。教師天地,94期,4~11。
 
系統版面圖檔 系統版面圖檔