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研究生:方怡凱
研究生(外文):I-Kai, Fang
論文名稱:利用比較蛋白質體學分析陰道鞭毛蟲對低溫環境之反應
論文名稱(外文):Comparative proteomics analysis of cold stress response in Trichomonas vaginalis
指導教授:鄧致剛鄧致剛引用關係
指導教授(外文):Petrus Tang
學位類別:碩士
校院名稱:長庚大學
系所名稱:基礎醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:英文
外文關鍵詞:Cold shockTrichomonas vaginalis2 dimensional electrophoresis
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Trichomoniasis, caused by Trichomonas vaginalis, is the most common non-viral sexual transmitted infection (STI) in the world. Cumulative evidences showed that this parasite can survive as a pseudocystic stage in external environment ubder different stress conditions and still infectious. This experiment investigated the protein expression patterns of T. vaginalis grow in normal and cold stress conditions (37℃ and 4℃) by using 2-dimensional electrophoresis (2DE) in order to understand how this parasite can survive at a critical temperature. Our results showed that enzymes related to energy production such as glyceraldehyde-3-phosphate dehydrogenase (G3PD) and fructose 1-, 6-biphosphate aldolase (FBPA) were expressed at a higher level under cold stress condition. The stress responsible proteins heat shock protein 70 (HSP70) and thiol peroxidase (Tpx) also have a similar expression pattern. One interesting observation is detoxification enzyme superoxide dismutase (SOD) which showed high mRNA expression in the Expressed Sequence Tags (EST) library but low protein expression level at low temperature. This observation indicated that importance of post transcriptional modification of mRNA in the regulation of protein expression. The present study also showed selective expression of members in multiple protein families such as malate dehydrogenase (MDH) and HSP70 in which only specific kind of isoforms are highly expressed at low temperature.
指導教授推薦書
口試委員審定書
授權書 iii
ACKNOWLEDGMENT v
中文摘要 vii
ABSTRACT viii
CHAPTER 1 INTRODUCTION 1
CHAPTER 2 MATERAL AND METHODS 13
CHAPTER 3 RESULTS 21
CHAPTER 4 DISCUSSIONS 26
CHAPTER 5 FIGURES 32
CHAPTER 6 TABLES 51
CHAPTER 7 REFERENCES 55
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