臺灣博碩士論文加值系統

腸病毒71型為微小病毒科(Picornaviridae)的成員，感染腸病毒71型會導致手口足病、疱疹性咽唊炎和腦炎等疾病。腸病毒71型2C蛋白和其他小RNA病毒科病毒相似性極高。科學家指出小兒麻痺病毒的2C蛋白跟病毒的複製有關。小兒麻痺病毒的2C蛋白會誘導細胞內膜重組和囊泡的堆積，影響細胞蛋白的運輸。本實驗室已經利用yeast two- hybrid的方法找出數個與腸病毒71型2C蛋白N端有作用的蛋白質。而我主要的研究目標為確認intersex like protein (IXL) 和 G-protein-coupled receptor 125 (GPR125)是否會和腸病毒71型2C蛋白N端作用。我們利用免疫螢光染色法和免疫沉澱法來驗證intersex IXL和GPR125與71型2C蛋白有交互作用。由實驗結果得知IXL不會和腸病毒71型2C蛋白N端作用。另一方面，由免疫沉澱實驗的結果顯示內生性的GPR125和腸病毒71型2C蛋白N端作用，並且由免疫螢光染色法得知GPR125與腸病毒71型2C蛋白共同分佈於內質網。

Enterovirus 71 (EV71) is a member of picornaviridae family. The major symptoms of enterovirus 71 infection included hand-food-and mouth disease, herpangina, and encephalitis. The 2C protein is highly conserved among all picornaviruses. Poliovirus 2C protein protein was required for viral replication. Poliovirus 2C protein induces intracelluar membranes rearrangement and vesicles accumulation. In our laboratory, we had found some putative cellular proteins that can interact with enterovirus 71 2C protein by yeast two-hybrid system. My project is using co-immunoprecipitation assay and indirect immunofluorescence assay to confirm the interaction between the N-terminal of enterovirus 71 2C protein and the putative cellular protein, intersex like protein (IXL) and G-protein-coupled receptor 125 (GPR125). In my data, I found that IXL can not interact with N-terminal of enterovirus 71 2C protein. However, we verify that N-terminal of enterovirus 71 2C can interact with GPR125 by co-immunoprecipitation assay. We further confirmed the interaction between GPR125 and N-terminal of enterovirus 71 2C protein by immunofluorescence assay. I found that both GPR125 and enterovirus 71 2C protein were colocalized in endoplasmic reticulum.

目錄
指導教授推薦書…………………………………………………
口試委員會審定書………………………………………………
碩士論文授權書…………………………………………………iii
誌謝………………………………………………………………iv
中文摘要…………………………………………………………v
英文摘要…………………………………………………………vi
目錄………………………………………………………………vii
縮寫表……………………………………………………………viii
緒論……………………………………………………………… 1
研究目的………………………………………………………… 11
材料與方法……………………………………………………… 12
結果……………………………………………………………… 19
討論……………………………………………………………… 27
圖表……………………………………………………………… 34
參考文獻………………………………………………………… 53
附錄……………………………………………………………… 58

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