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研究生:李晉鳴
研究生(外文):Jim-Ming Lee
論文名稱:秀麗隱桿線蟲NCL-1與FIB-1在早期胚胎反比例表現與無核仁性狀之相關性
論文名稱(外文):The inversed expression of FIB to NCL-1 correlates with the anucleolate phenotype of early embryo in Caenorhaditis elegans
指導教授:羅時成羅時成引用關係
指導教授(外文):Szecheng John Lo
學位類別:碩士
校院名稱:長庚大學
系所名稱:基礎醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:英文
論文頁數:56
中文關鍵詞:秀麗隱桿線蟲無核仁核糖體野生型細胞質蛋白質反比例
外文關鍵詞:Caenorhaditis elegansanucleolatebeforefamily
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秀麗隱桿線蟲有一個名為異常核仁 (ncl-1) 的基因,它是屬於調控許多生物生長與分化的NHL家族成員。在已經知道功能的家族裡,有一個稱為lin-41的秀麗隱桿線蟲的基因,被認為與控制後轉錄修飾有關。不過,LIN-41及其他NHL蛋白質作用在後轉錄修飾的機制還不清楚。以前的研究已經顯示ncl-1在秀麗隱桿線蟲裡與抑制細胞生長和核糖體合成有關。相較於野生型 (N2),喪失ncl-1功能的秀麗隱桿線蟲有較大的核仁、較大的細胞,以及28S、18S與5S rRNA的高速率轉錄。為了要了解核仁形成與NCL-1的關係,我們檢查NCL-1的表現與熟知的核仁蛋白fibrillian (FIB-1)之間的關係。在不同發育階段線蟲的RNA 分析結果顯示,胚胎時期ncl-1 表達量比其它階段高。有趣的,相較於野生型胚胎時期FIB-1蛋白質在ncl-1突變株有明顯的上升,但是mRNA卻沒有差異。藉由細胞組成分離實驗得知NCL-1主要位於細胞質,並未在細胞核內發現。ncl-1突變株除了在胚胎時期有核仁的差異以外,本篇論文裡面新發現此突變株在延緩成蟲卵子核仁的消失,直到細胞核膜分解之前。總結本篇論文,胚胎時期NCL-1的出現抑制了FIB-1表現與核仁形成。至於NCL-1的功能是否為直接抑制核仁蛋白的轉譯,是未來必須去探討的。
The abnormal NuCLeoli (ncl-1) gene of Caenorhabditis elegans encodes a member of the conserved NHL family of proteins, which appear to regulate differentiation and growth in a variety of organisms. Previous studies have shown that NCL-1 is located in the cytoplasm and functions as an inhibitor of cell growth and ribosome synthesis. Interestingly, the most salient feature in ncl-1 loss of function mutants occurs in the nucleus—enlargement of nucleolar size. Whether the oocytes of ncl-1 mutant exhibite a delayed disappearance of nucleolus until just before nuclear nvelope breakdown (NEBD) has not been demonstrated yet. To gain more insight into the role of NCL-1 in the nucleolus disassembly and reformation, the expression of ncl-1 at different developmental stages of C. elegans and correlation with a well-known nucleolar protein, fibrillian (FIB-1), were examined. RNA analysis revealed that in the wild type strain, N2, the relative amount of ncl-1 transcripts was significantly higher in embryos than in the later stages of development. On the other hand, the fib-1 expression patterns in N2 and ncl-1 mutant were alike during all stages of development. Whereas the amount of fib-1 transcript in the ncl-1 embryo remained comparable to that of the N2, immunoblot analysis showed that the protein level of FIB-1 was higher in the ncl-1 embryo. These findings suggest that NCL-1 inhibits FIB expression in the posttranscription level at the embryonic stage. Whether NCL-1 function as a translation inhibitor remains to be explored.
摘要 ……………………………………………………………………1
Abstract ….………………….………………………………………...2
Introduction ……………………………………………………………..3
Material and Methods ………………………………………………….16
Results ………………………………………………………………..26
Discussion ……………………………………………………………...30
References ……………………………………………………………...35
Figures ………..………………………………………………………40
Appendix ……………………………………………………………….53
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