臺灣博碩士論文加值系統

祕魯酸漿(Physalis peruviana L.)是一種民間常用的生藥。經由超臨界二氧化碳流體萃取方式(supercritical fluid extraction/carbon dioxide; SFE-CO2)取得三種不同的祕魯酸漿超臨界二氧化碳萃取物包括SCEPP-0, SCEPP-4 及SCEPP-5。本研究為探討這些超臨界萃取物的總多酚及類黃餇含量，抗氧化及抗發炎活性與水及酒精萃取物的結果比較分析及比較這些超臨界萃取物、水及酒精萃取物之總多酚(total polyphenol)及類黃酮(flavonoid)濃度、抗氧化及抗發炎的活性。實驗結果顯示超臨界祕魯酸漿萃取物(SCEPP-5)具有最高之總多酚 (90.80±2.21 mg/g)及類黃酮(234.63± 9.61 mg/g) 含量。在濃度0.1~30 μg/mL之SCEPP-5顯示具有最強清除超氧陰離子(．O2-)的活性及抑制黃嘌呤氧化酶(XOD)效力。此外30 ~50 μg/mL濃度下，SCEPP-5顯著的可預防脂多醣(LPS)對鼠巨噬細胞(Raw 264.7)誘導之細胞毒殺效應。在10～50 μg/mL，SCEPP-5亦具有顯著的抑制脂多醣(LPS)所誘導之一氧化氮(NO)的釋放與前列腺素E2 (PGE2)之形成，並呈現劑量效應的關係。同時，30 μg/mL之SCEPP-5可有效地阻擾脂多醣(LPS)所誘導的iNOS及COX-2表現。綜合以上結果，超臨界萃取物SCEPP-5顯示具有最強的抗氧化及抗發炎活性，其抑制脂多醣(LPS)所誘導發炎的機制可能經由抑制iNOS與COX-2的表現。

Physalis peruviana L. (PP) is a medicinal herb widely used in folk medicine. Supercritical fluid extraction/carbon dioxide (SFE-CO2) method was employed to obtain three different PP extracts, namely SCEPP-0, SCEPP-4 and SCEPP-5. The total phenol and flavonoid concentrations, as well antioxidant and anti-inflammatory activities of these extracts were analyzed and compared them with aqueous and ethanolic PP extracts. Among all the extracts tested, SCEPP-5 demonstrated the highest total phenol (90.80±2.21 mg/g) and flavonoid (234.63± 9.61 mg/g) contents. At concentrations 0.1 to 30 μg/mL, SCEPP-5 also demonstrated the strongest superoxide anion scavenging activity and xanthine oxidase inhibitory effect. At 30 μg/mL, SCEPP-5 significantly prevented lipopolysaccharide (LPS)-induced cell cytotoxicity in murine macrophage (Raw 264.7) cells. At 10~50 μg/mL, it also significantly inhibited LPS-induced NO release and PGE2 formation in a dose-dependent pattern. SCEPP-5 at 30 μg/mL remarkably blocked the LPS induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Taken together, these results suggest that SCEPP-5, an extract of SFE-CO2, displayed the most potent antioxidant and anti-inflammatory activities. It protection against LPS-induced inflammation could be through the inhibition of iNOS and COX-2 expression.

目 錄
中文摘要………………………………………………………………I
英文摘要……………………………………………………………………II
目錄……………………………………………………………………III
圖次…………………………………………………………………………V
縮寫表………………………………………………………………………VI
第一章 緒論…………………………………………………………1
第二章 材料與方法……………………………………………………………8
祕魯酸漿酒精粗萃取物之製備…………………………………8
祕魯酸漿熱水萃取物之製備……………………………………9
祕魯酸漿超臨界萃取物之製備……………………………………9
實驗方法……………………………………………………………9
類黃酮含量測定……………………………………………………9
多酚含量測定…………………………………………………10
祕魯酸漿超臨界萃取物之抗氧化作用研究…………………10
Cytochrome c測定……….………………………………………10
黃嘌呤氧化酶(XOD)活性之測定………………………11
細胞培養…………………12
細胞存活率分析法(MTT assay)…………………………………12
一氧化氮(NO)之含量測定………………………………………13
前列腺素E2 (PGE2)之含量測定………………………………13
西方墨點法(Western blot)分析………………………13
統計分析……………………………………………………………14
儀器設備…………………………………………………………15
第三章 實驗結果……………………………………………………………16
第四章 討論………………………………………………………………20
第五章 結論………………………………………………………………23
參考文獻……………………………………………………………24
圖表………………………………………………………………33
研究成果與業績..............................................39

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