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研究生:林銘璋
研究生(外文):Ming-Jang Lin
論文名稱:蘇力菌素防治水稻田福壽螺之探討
論文名稱(外文):Study on the Control of Pest Snail Pomacea canaliculata in Paddy field by thuringiensin containing fermentation broth from Bacillus thurngiensis
指導教授:曾耀銘曾耀銘引用關係
指導教授(外文):Yew-Min Tzeng
學位類別:碩士
校院名稱:朝陽科技大學
系所名稱:應用化學系碩士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:67
中文關鍵詞:蘇力菌素福壽螺
外文關鍵詞:thuringiensinPomacea canaliculata
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研究探討使用內含蘇力菌素(thuringiensin)之發酵液,防治水稻田福壽螺(Pomacea canaliculata)之可行性。其中,室內劑量檢定試驗於2003年進行,該室內試驗部分用29 x 38公分的塑膠盆內裝田土約10公分,加水深約3公分模擬水田進行劑量檢定,室內試驗蘇力菌素發酵液施用濃度分別為50、100、200、300、400 ppm等五種處理,三重複。室內試驗結果施用濃度100、200 ppm其福壽螺平均致死率分別為50%與100%,規劃室外田間試驗施用濃度分別為100、200 ppm。插秧後七天內以塑膠浪板圈圍面積3 m2的範圍進行,蘇力菌素發酵液施用濃度分別為100、200 ppm等兩種處理,另以70%耐克螺可濕性粉劑和苦茶粕為對照藥劑,三重複,於2005年進行試驗結果得知,二期作插秧後施用對照藥劑70%耐克螺可濕性粉劑和苦茶粕於第三天進行調查,福壽螺平均致死率均為100%,施用內含蘇力菌素發酵液濃度100、200 ppm於第六天進行調查,福壽螺平均致死率分別為53.3%與86.7%。
The purpose of the study is to test the thuringiensin (β-exotoxin) from Bacillus thuringiensis for controlling Pest Snail Pomacea canaliculata. Experiments were carried out both in the laboratory and in paddy field. The laboratory test started in 2003, the 29 x 38 cm plastic basins with 10 cm high of soil and 3 cm deep of water were used to imitate paddy field. The compositions of application of thuringiensin containing fermentation broth of Bacillus thuringiensis were 50, 100, 200, 300, 400 ppm with 3 replicates. Paddy field test started in 2005, areas of 3 m2 encircled by plastic board were used for this test. The laboratory test results showed that after using thuringiensin containing fermentation broth of Bacillus thuringiensis, the average death rates of the Ampullarium Canaliculatus were 50% and 100% for the 100 and 200 ppm treatments, respectively. Seven days after transplanting, thuringiensin containing fermentation broth were applied in the test spots. The composition of application of thuringiensin containing fermentation broth were 100 ppm and 200 ppm 3 replicating, in the first and the second rice crop of 2005. The 70% W.P. Niclosamide and tea-seed dregs were used as a chemical controls. The chemical controls that after using 70% W.P. Niclosamide and tea-seed dregs, the average death rates of the Ampullarium Canaliculatus were 100% both treatments after three days, respectively. The results showed that after using thuringiensin containing fermentation broth of Bacillus thuringiensis, the average death rates of the Ampullarium Canaliculatus were 53.3% and 86.7% for the 100 and 200 ppm treatments after seven days, respectively.
目錄
書名頁
論文電子檔案上網授權書
授權書(博碩士論文)
論文口試委員會審定書
致謝
中文摘要.......................................................II
英文摘要.......................................................III
目錄...........................................................IV
表目錄........................................................VII
圖目錄.......................................................VIII

第一章 序論....................................................1
1-1 前言.......................................................1
1-2 研究目的...................................................5

第二章文獻回顧.................................................6
2-1 蘇力菌素簡介...............................................6
2-2 福壽螺簡介................................................13

第三章劑量檢定與試驗設計
3-1 前言.......................................................31
3-2 供試藥劑...................................................31
3-3 動物來源...................................................31
3-4 試驗材料與方法.............................................32
3-5 結果與討論.................................................33

第四章實驗材料與方法..........................................41
4-1 供試藥劑..................................................41
4-2 動物來源..................................................42
4-3 試驗材料與方法............................................42

第五章結果與討論..............................................44

第六章結論與展望..............................................51
附錄..........................................................61
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