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研究生:李啟銘
研究生(外文):li chi ming
論文名稱:以生物可降解性聚已內酯包埋溶磷菌Bacillussp.strainPG01進行微生物釋放與性質之研究
論文名稱(外文):Cell-release and properties of polycaprolactone composites encapsulating phosphate-solubilizing bacterium Bacillus sp. Strain PG01
指導教授:吳進三
學位類別:碩士
校院名稱:高苑科技大學
系所名稱:高分子環保材料研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
中文關鍵詞:生物可分解材料溶磷菌
外文關鍵詞:biodegrationbacillus
相關次數:
  • 被引用被引用:2
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  • 收藏至我的研究室書目清單書目收藏:1
中文摘要
本論文主要研究生物可分解材料PCL、PCL/Starch合膠及PCL/HA合膠,包覆溶磷菌(PBS),發展控制微生物肥料釋放之可行性。經由菌體釋出之PCL、PCL/Starch合膠及PCL/HA合膠表面型態上觀察到,溶磷菌釋出30~60天後的包覆材料表面結構出現裂痕,且配合釋放測試。顯示包覆材料之菌體釋放性為PCL/Starch (20Wt.%) > PCL alone > PCL/HA (20Wt.%)。由表面型態分析結果;得知PCL/Starch合膠因菌體釋放之裂痕,因而在熱分析測試中,發現熔融焓值上升。這是因聚已內酯/澱粉合膠包覆材料中,添加Starch能提高包覆材料之菌體釋放性,且Starch成為菌體生長之營養源,導致熔融焓值大量上升。並相較於PCL/HA合膠,因HA在複合材中形成膠狀物質,因而在熱性質分析中,測得熔融焓值微量下降,所以造成低菌體釋放之主因。故由本研究之結果建議,適當的改變菌體包覆之添加材料,可以達到控制微生物肥料釋放之目的。
英文摘要
  This study examined the feasibility of using polycaprolactone (PCL) and its composites (with Starch and/or HA) encapsulating cells of phosphate-solubilizing bacteria (PBS) for the development of biodegradable and “controlled-release” bacterial fertilizer. The PSB used in this work was able to degrade all the cell-release. Morphology observation indicates that severe disruption of the capsule structure occurred after incubation for 30-60 days. The biodegradability of the capsules decreased in the order of PCL/Starch (20Wt.%) > PCL alone > PCL/HA (20Wt.%). Similar trends were also observed for the decrease in enthalpy (△Hm), suggesting strong connections between biodegradability and the thermal properties.
  Addition of Starch appeared to enhance the biodegradability of the capsules,whereas the HA-blended composites were less biodegradable. The amount and rate of cell release from cell-encapsulated PCL-based capsules were positively dependent on the biodegradability and on the decrease in the thermal enthalpy of melting. The cell-release profiles were, however, quite similar for all types of capsules. The outcome of this work seems to suggest that by proper manipulation of composite composition, controlled release of the bacterial fertilizer (i.e.,Bacillus sp. PG01 cells) may be achievable.
總目錄
中文摘要………………………………………………………………………Ⅰ
英文摘要………………………………………………………………………Ⅱ
誌謝……………………………………………………………………………Ⅲ
總目錄…………………………………………………………………………IV
表目錄………………………………………………………………………....VI
圖目錄………………………………………………………………………..VII

第一章 前言…………………………………………………….……………...1

第二章 文獻回顧………………………………………….…………………...4
2.1 高分子分解………………………………………………….………..4
2.2 完全生物可分解性高分子……………………………………..…….6
2.3 聚已內酯……………………………………………………………...9
2.4 生物可分解添加材料Starch及HA之介紹…………………….…10
2.5 生物可分解材料包覆菌體溶磷菌之介紹………………………….16

第三章 實驗…………………………………………………………………..18
3.1 實驗藥品…………………………………………………………….18
3.2 實驗儀器…………………………………………………………….19
3.3 實驗流程…………………………………………………………….21
3.4 實驗步驟…………………………………………………………….22
3.5 分析與鑑定………………………………………………………….26

第四章 結果與討論…………………………………………………………..29
4.1 菌體的生長曲線…………………………………………………….29
4.2 菌體在包覆材料中之釋放生長情形曲線………………………….31
4.3 熱學性質分析……………………………………………………….37
4.4 表面型態觀察……………………………………………………….52
4.5 凝膠滲透層析分子量分析………………………………………….63
4.6 X光繞射分析……………………………………………………….70

第五章 結論…………………………………………………………………..73
參考文獻………………………...…………………...………………………..75
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