臺灣博碩士論文加值系統

摘　要

本研究成功地利用微機電系統製程技術，發展可即時血糖監控並自動化胰島素注射的微流體系統，此微流體系統包含一微流體晶片(Microfluidic Chip)和可攜式的微流體控制器，其中包含空氣壓縮機、幫浦可程式控制系統和電磁閥。微流體晶片由上板微流體操控元件和下板感測電極兩個部分封裝完成，上板微流體操控元件包含微流管道、氣動式微幫浦與微閥門，係以聚二甲基矽氧烷（Polydimethylsiloxane, PDMS）材料製作，利用微流體控制器將壓縮空氣注入PDMS薄膜中，使PDMS薄膜產生幫浦和閥門的效果，下板感測電極包含葡萄糖感測電極和流速感測電極，葡萄糖電極是定電流的方式，利用導電性高分子材料，將葡萄糖氧化酵素（GOD）電聚合在鉑電極上以進行電化學檢測，再將測得的葡萄糖值換算成血糖的濃度，以進行血糖的監控。
和傳統的血糖檢測儀器比較，本系統除了能夠連續地檢測血糖濃度之外，搭配本晶片中商用的留滯針、新型微幫浦和微閥門等微流體控制元件和流速感測器，可以自動精準的進行胰島素注射，以保持血液中的葡萄糖濃度在正常的範圍。
本研究希望能藉由此系統的發展，省去過去糖尿病病患使用血糖計測量完之後仍須自行進行胰島素注射的種種不便，改善眾多糖尿病病患其生活品質。

Abstract

This paper presents a new microfluidic system capable of real-time measurement of glucose concentration and automatic insulin injection. The microfluidic system is composed of a microfluidic chip, a measurement and control circuit system, a compressed air source, and several electromagnetic valves to form a handheld system. The microfluidic chip is fabricated by using microfluidic techniques comprising of glucose sensing electrodes, a flow sensor, and polydimethylsiloxane (PDMS)-based microfluidic structures such as micropumps, microvalves, and microchannels. Commercially available needles are incorporated for continuous glucose monitoring and long-term insulin injection. The microfluidic system performs a variety of processes including blood sample collection, glucose concentration detection, and injection of insulin. Micropumps and microvalves are used to perform the whole process automatically. The valve switching and pumping effects are generated utilizing compressed air to deform thin PDMS membranes. Compared with traditional glucose monitoring platforms, the developed system can be used for on-line monitoring of glucose concentration and precise injection of proper doses of insulin, in order to maintain a stable glucose concentration in human blood. The developed microfluidic system could become a crucial tool for diabetes patients in the future.

目　錄

摘要　i
Astract　iii
誌謝　v
目錄　vii
表目錄　x
圖目錄　xi
縮寫及符號說明　xiv

第一章 緒論
1-1　前言　1
1-2　生醫微機電系統簡介　2
1-3　研究動機與目的　4
1-4　研究方法　5
1-5　文獻回顧　7
1-5-1　微幫浦文獻回顧　７
1-5-2　微閥門文獻回顧　15
1-5-3葡萄糖生物感測器文獻回顧　18
1-6 論文架構　20

第二章　理論
2-1　微流體操控系統　23
2-2　流速感測器　26
2-3　葡萄糖生物感測器　28
　2-3-1 生物感測器　28
　2-3-2 電化學傳感器　31
2-4　葡萄糖感測原理　33
2-5　酵素固定的方法　34
2-6　電聚合導電高分子酵素固法　37

第三章　晶片之設計及製作
3-1　血糖檢測及自動胰島素注射晶片設計　40
3-2　製程規劃與材料選擇　43
3-3　光罩製作　45
3-4　晶片製程　46
　3-4-1　晶片表面清洗　46
　3-4-2　微影製程　47
　3-4-3　金屬薄膜成沉積製程　52
　3-4-4　AZ4620製程與剝離製程　53
　3-4-5　SU-8母模製程與PDMS注模技術　56
3-5　晶片接合與封裝　61

第四章　實驗架設
4-1　微流體晶片實驗架設　65
4-2　流速感測實驗架設　66
4-3　血糖感測實驗架設　67

第五章　結果與討論
5-1　微氣動幫浦和微閥門效能測試　68
5-2　流速感測器測試　74
5-3　葡萄糖樣品測試　75
5-4　血液樣品測試 77
5-5　胰島素注射結果與分析　82

第六章　結論與未來展望
6-1　結論　88
6-2　未來展望　89

參考文獻　91
自述　98
著作　99

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