臺灣博碩士論文加值系統

近幾年來類磷脂質高分子在生醫科技的應用上具有舉足輕重的地位，其優異的生物相容性以及類似於生物細胞膜的組成等特性都是備受矚目的。MPC高分子抗凝血的特性在各應用上都有不錯的研究成果，如生物晶片、人工血管、組織修復、藥物控制釋放等等。本論文主要是要研究以聚PLA-PMPC雙團聯共聚物，以作為治療癌症之奈米藥物載體。
在一系列的實驗中，聚乳酸(PLA)與MPC以原子轉移自由基聚合法合成PLA-PMPC。以透析法製備成高分子微胞，探討溶劑與粒徑的關係，改變溶劑組成比來獲得不同粒徑的微胞。利用1H-NMR、AFM、以及界面電位等測定出微胞的型態與結構。以HFW與Hela兩株細胞測試材料以及高分子微胞之細胞毒性，證實材料具有優異之生物相容性以及安全性。包覆疏水性抗癌藥物Doxorubicin，將其包覆情形、藥物釋放的行為、以及毒殺的效果等結果進行比較，探討此合成之高分子材料在治療癌症之藥物控制釋放系統上之應用價值。

中文摘要…………………………………………………………………i
英文摘要………………………………………………………………ii
誌謝…………………………………………………………………………iii
目錄……………………………………………………………………iv
圖索引…………………………………………………………………vi
表索引…………………………………………………………………viii
第一章 研究背景與動機………………………………………………1
第二章 相關理論與文獻………………………………………………4
2.1 含磷脂質材料簡介……………………………………………4
2.1.1 MPC單體……………………………………………4
2.1.2 含磷脂質單體—MPC之特性………………………4
2.2 含磷脂質高分子具有優異生物相容性之機制………………7
2.2.1 高分子含水之結構……………………………………7
2.2.2 磷脂質吸附於高分子表面……………………………7
2.2.3 蛋白質吸附於高分子表面…………………………7
2.2.4 蛋白質吸附數量……………………………………7
2.2.5 高分子自由水含量和蛋白質吸附的關係…………7
2.2.6 高分子表面吸附磷脂質和蛋白質之關係…………8
2.3 生物可降解性高分子………………………………………9
2.3.1 生物可降解定義……………………………………9
2.3.2 生物可降解機制……………………………………9
2.3.3 生物可降解性高分子簡介…………………………10
2.4 高分子微胞在藥物制放上的應用…………………………12
2.5 陰離子聚合反應原理………………………………………14
2.5.1 起始劑之種類………………………………………14
2.5.2 起始反應之方式……………………………………14
2.5.3 成長反應……………………………………………15
2.5.4 無終止反應與其特色………………………………15
2.6 原子轉移自由基聚合(ATRP) ………………………………16
2.6.1 起始反應機制………………………………………16
2.6.2 適用之單體…………………………………………16
2.6.3 觸媒與配位基………………………………………17
2.6.4 多元的聚合法與其特性……………………………18
第三章 實驗方法………………………………………………………21
3.1 實驗藥品……………………………………………………21
3.2 實驗儀器與裝置……………………………………………21
3.3 合成PLA-PMPC雙團聯共聚物……………………………22
3.4 鑑定與分析…………………………………………………23
3.5 臨界微胞濃度………………………………………………23
3.6 製備PLA-PMPC高分子微胞………………………………23
3.7 粒徑與介面電位分析………………………………………24
3.8 體外藥物釋放模擬…………………………………………24
3.9 細胞存活率與細胞毒殺實驗………………………………25
第四章 結果與討論……………………………………………………26
4.1 合成PLA-PMPC兩團聯共聚物……………………………26
4.2 臨界微胞濃度………………………………………………30
4.3 粒徑與介面電位分析………………………………………32
4.3.1 透析法製備高分子微胞……………………………32
4.3.2 表面電位分析………………………………………38
4.4 細胞存活率…………………………………………………39
4.5 體外藥物釋放模擬…………………………………………41
4.6 細胞毒殺實驗………………………………………………44
第五章 結論……………………………………………………………45
參考文獻………………………………………………………………46

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