臺灣博碩士論文加值系統

鹽分逆境往往會造成植物內生性多元胺的累積，但對多元胺生合成基因的表現則不甚了解。本實驗利用鹽敏感型水稻台農67號 (Oryza sativa L. cv. Tainung 67) 及耐鹽型水稻台中178號 (Oryza sativa L. cv. Taichung 178)兩種耐性不同的稉稻( japonica type )懸浮細胞作為實驗材料，探討在150 mM NaCl鹽分逆境下多元胺生合成基因的表現及其含量變化，以了解水稻在鹽逆境下可能的生理變化及耐鹽機制。
根據本實驗的結果認為鹽分逆境能有效增加兩種耐性不同的水稻adc的表現量，因而增進Putrescine(Put)的累積；同時耐鹽性水稻能在逆境下維持較高及較久的adc表現量，推測是其抵抗鹽逆境的機制之ㄧ。至於samdc則在兩種水稻品系遭遇鹽分逆境下都有明顯表現，表示合成spermidine(Spd)所需的aminopropyl group供給無虞。然而台中178號在鹽逆境下並未提高Spd的含量，台農67號則在遭遇鹽分逆境時，能誘導spd syn的基因表現而增加Spd的含量。由於本實驗是以懸浮細胞當做材料，但水稻懸浮細胞基因的表現與植株的表現並無直接相關性，所以可能因此顯現出與過去研究上較不相同的實驗結果，也可能是水稻懸浮細胞的多元胺生合成相關基因有著不同的調控機制。根據上述實驗結果推測samdc基因的表現在Spd累積上並未扮演關鍵角色。
另外，由實驗中亦可證實，odc存在於水稻的genomic DNA中。然而水稻遭受鹽分逆境時卻傾向adc的表現，但由於無法偵測到odc的表現情況，因此推測逆境不易誘導odc表現。

The effects of salt stress (150 mM NaCl) on endogenous levels of polyamines were studied in the suspension rice cell lines of salt-tolerant (Oryza sativa L. cv. Taichung 178) and salt-sensitive (Oryza sativa L. cv. Tainung 67) cultivars. The changes in levels of polyamines and in transcripts of polyamine metabolic enzymes under salt stress were investigated. Salt stress significantly increased the levels of putrescine(Put) and adc mRNA in both rice cultivars measured along with time. The levels of spermidine(Spd) as well as those of spd syn mRNA were significantly increased in the salt-sensitive cultivar ( Tainung 67)。However, no significant changes in the salt-tolerant cultivar ( Taichung 178) under saline conditions. These results suggested that different suspension cell lines of rice were regulated differently under salt stress. However, gene expression of samdc revealed no significant effect on accumulation of Spd. Besides, although I could detect odc genes in both rice genomes, no odc cDNA were detected in both cultivars, suggesting that odc gene expression might be irrelevant to tolerance toward salt stress.

目錄
縮寫表 ……………………………………………………..1
英文摘要 …………………………………………………..2
中文摘要 …………………………………………………..3
壹、前言 …………………………………………………..5
貳、前人研究 ………………………………………………7
一. 鹽分逆境對植物的危害 ……………………………………7
二. 植物抵抗鹽分逆境的機制 …………………………………8
三. 多元胺的功能及代謝途徑 ………………………………10
四. 鹽分逆境下植物的多元胺生合成酵素表現………………13
参、材料與方法 ………………………………………………….19
肆、結果 ………………………………………………………….30
一. 鹽分逆境對不同耐鹽性水稻懸浮細胞鮮重之影響 ……30
二.鹽分逆境下不同耐鹽性水稻懸浮細胞多元胺含量變化…30
三. 鹽分逆境下不同耐鹽性水稻懸浮細胞的多元胺生合成基因表現 ………………………………………………………31
伍、討論 ……………………………………………………………54
一. 鹽分逆境對不同耐性的水稻懸浮細胞鮮重的影響 ……54
二.鹽分逆境下不同耐鹽性水稻懸浮細胞的多元胺含量變化 55
三. 鹽分逆境下不同耐鹽性水稻懸浮細胞的多元胺生合成基因表現 ………………………………………………………59
陸.結論 ……………………………………………………………69
柒.參考文獻 ………………………………………………………70
捌.附錄 ……………………………………………………………...79
附錄一 、多元胺在植物中的代謝途徑 .........82
附錄二、各基因之引子(primer)序列 .........83
附錄三 、N6D培養基成分 …………………………84

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