臺灣博碩士論文加值系統

CD200是一種表面膜蛋白，具有兩個免疫超族群區塊，目前的研究認為，透過這個免疫超族群區塊與其他細胞表面的蛋白鍵結，可用來驅動其他細胞表現各種功能。CD200分布非常廣泛，存在白血球、內皮細胞上、神經以及其他的細胞種類。目前的發現CD200的特色是與免疫反應有關。因此，CD200普遍認為是一種免疫表現反應的分子。然而CD200也是一種黏著分子，可以利用免疫超族群區塊進行細胞與細胞間的連結。同時，是否這樣在上皮系統中CD200在其細胞間的鍵結中，扮演了什麼角色，目前仍不清楚。但是，在我們的研究發現，CD200的確表現在大部分的內分泌腺體中，特別是位在唾液腺以及乳腺中，同時發現CD200的分佈有其特殊的位置與部位。免疫電子顯微鏡更進一步的證實，CD200位在肌上皮細胞上。而這些細胞顯示出明顯的CD200的免疫反應在其細胞膜及細胞膜穴狀內陷上，有趣的是CD200分布在靠基底板的細胞膜及細胞膜穴狀內陷(caveolae)上，而與腺細胞緊貼的細胞膜及細胞膜穴狀內陷則沒有表現CD200。我們將顎下腺與舌下腺的輸出管道出口用漨線予以結紮後肌上皮細胞會過度增殖且增加其cd200的表現。

CD200 is a membrane glycoprotein that contains two immunoglobulin superfamily domains and it functions through interactions with other cell-surface proteins. CD200 has broad distribution and present on leucocytes, endothelial cells, neurons and some other cell types. The recent characterization of CD200 suggests that CD200 may regulate immune responses. Up to now, CD200 is concluded as an immunosuppressive molecule. However, CD200 may also be an adhesion molecule and bind between cell and cell with IgG domain. Whether CD200 expression could function in the cell-cell adhesion in the epithelial system has not yet been determined. Our preliminary data showed that CD200 does exist in most exocrine glands, particularly in the salivary and mammary glands of which expressions varied and were dependent on their organ types. Immuno-electron microscopic data further revealed that CD200 was localized on the myoepithelial cells. The cells showed significant CD200 immunoreactivities on their cytoplasmic membrane and caveoli dramatically on those facing the basal lamina, in contrast to those intimately contacting with acinar cells being absent of CD200. During the atrophy of duct-ligated submandibular and sublingual glands, myoepithelial cells became hyperplasia and stronger CD200 immunoreactivities.

目錄 I
圖目錄 IV
中文摘要 V
英文摘要 VI
第一章 緒言 1
第一節 CD200 1
壹、CD200的發現 1
貳、CD200的相關研究 1
第二節 黏著分子 6
壹、黏著分子 6
貳、免疫球蛋白超家族 7
第三節 外分泌腺 9
壹、外分泌腺 9
貳、唾液腺發育 9
參、唾液腺一般結構 10
肆、三種主要唾液腺的結構特點 13
第四節 肌上皮細胞 15
壹、肌上皮細胞的特徵 15
貳、肌上皮細胞的發育 16
第二章 研究目的 19
第三章 材料與方法 20
實驗材料 20
壹、實驗動物 20
貳、實驗藥品 20
參、免疫組織化學染色抗體 20
肆、免疫螢光染色抗體 20
伍、其他化學試劑 21
陸、儀器設備 22
實驗動物準備 23
實驗組 23
對照組 23
冷凍切片之動物犧牲與標本組織取得 23
電子顯微鏡切片之動物犧牲與標本組織取得 24
免疫組織化學染色 24
免疫螢光染色 ( Immunofluorescent staining ) 26
陸、對比染色 ( Counter staining ) 及脫水 ( Dehydration ) 27
柒、電子顯微鏡切片之標本備製與切片 27
捌、統計分析 28
第四章 結果 29
CD200在大白鼠各唾液腺體的分佈情形 29
利用電子顯微鏡觀察CD200在正常大白鼠頷下腺的分佈情形 31
觀察大白鼠唾液腺結紮後，CD200的表現情形 33
CD200在唾液腺結紮後七十二小時的分佈情形 33
利用電子鏡觀察唾液腺不同時間點結紮後，CD200的表現情形 34
統計計算 35
第五章 討論 37
第一節 肌上皮細胞在唾液腺上的表現與形態 37
第二節 肌上皮細胞CD200特殊分佈與表現 42
第三節 腺體結紮與CD200 47
第六章 結論 50
參考文獻 79

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