跳到主要內容

臺灣博碩士論文加值系統

(44.220.62.183) 您好!臺灣時間:2024/02/27 21:37
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:陳仁富
研究生(外文):Chen, Jen-Fu
論文名稱:飼育不同溫度與鹽度培養之龍鬚菜對九孔免疫抗病反應探討
論文名稱(外文):Studying on feeding various temperature and salinity treated Graciliria tenuistipitata and how it affect the (following) immunity and antidisease ability of abalone in Tawan
指導教授:翁韶蓮翁韶蓮引用關係
指導教授(外文):Wong, Saou-Lien
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:水產養殖系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:52
中文關鍵詞:九孔龍鬚菜酚氧化酵素超氧根離子
外文關鍵詞:AbaloneGracilaria tenuistipitataphenoloxidaseO2-
相關次數:
  • 被引用被引用:0
  • 點閱點閱:598
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:2
龍鬚菜(Gracilaria tenuistipitata)為台灣養殖九孔主要之餌料,且大型藻中所含有大量的多醣物質已被證明具有增強生理免疫抗病反應之功能。而藻類藻體內之營養鹽含量亦會因外界環境不同而有所不同。本研究將龍鬚菜以不同鹽度及溫度來培養,再以這些在不同環境下培養之龍鬚菜餵食九孔,並測其九孔體內血球酚氧化酵素(PO)、超氧根離子(O2-)免疫抗病反應,來觀測龍鬚菜在何種溫度及鹽度環境下對九孔之免疫反應提升有較佳的效果。由藻類培養結果找出龍鬚菜的最適溫度約在15 ℃~ 25 ℃之間,而鹽度範圍大致在15‰ ~29‰。然而在九孔免疫生理反應實驗中,則以餵食於溫度25 ℃~30 ℃,鹽度29‰、36‰培養之龍鬚菜有較佳的誘發免疫反應出現。因此,由本實驗的結果來評估考慮九孔之免疫抗病能力則餵食九孔之龍鬚菜最適合之培養環境應該在溫度20 ℃~ 25 ℃間,過高的溫度會使藻類培養不易,而鹽度則以29‰~36‰間為佳,過高或過低的鹽度培養對調節九孔之免疫機制上則較無明顯的反應。
Gracilaria tenuistipitata is major feed for abalone cultivation in Taiwan. Polysaccharides are already known to have the ability to stimulate the immune system from macroalgae. To understand how macroalgae quality affect the immune ability of abalone. There were 4 different temperatures and 4 salinities used to set up 20 environmental combinations to test growth of G. tenuistipitata and used as feed for abalone thereafter. This studies that breeding G. tenuistipitata by difference salinity and temperature and feed G. tenuistipitata by these under different environment cultured to abalone. The results showed that, measurement of in vivo hemocyte phenoloxidase (PO) and the ultra oxygen root ion (O2-) the immunity disease-resistant response from abalone indicated that G. tenuistipitata seemed to immune stimulate effect of abalone under what kind of temperature and the salinity environment has a better the immune response promotion. The optimal growth temperature and salinity ranged between 15 ℃ and 25 ℃, and 15‰ to 29‰ superlatively. However, the best temperature treatment of thalli to result in immunity physiological reaction of abalone was 25℃~30℃, while, it was 29‰~36‰ from salinity treatment. That is, it required higher temperature and salinity to result in better immunity physiological reaction of abalone for utilization of cultured G. tenuistipitata.
目錄
摘要................................................................I
Abstract..........................................................III
誌謝................................................................V
目錄...............................................................VI
圖表目錄............................................................IX
第1章 緒論...........................................................1
1.1 台灣鮑魚之分類與分佈.............................................. 1
1.2 九孔防禦機制......................................................2
1.2.1 細胞性防禦作用..................................................2
1.2.2 體液性防禦作用..................................................5
1.3 龍鬚菜的分類與分佈.................................................5
1.4 環境因子與大型藻之關係.............................................6
第2章 材料與方法......................................................9
2.1 龍鬚菜藻體與九孔取得...............................................9
2.2 藻體培養與成長適應.................................................9
2.2.1 成長適應試驗....................................................9
2.2.2 藻體蛋白質、游離胺基酸分析........................................9
2.2.3 藻體內硝酸分析.................................................10
2.2.4 藻體硝酸還原酵素分析............................................10
2.3 九孔免疫反應試驗.................................................12
2.3.1 酚氧化酵素之測定...............................................12
2.3.2 超氧化離子之測定...............................................12
第3章 結果..........................................................14
3.1 龍鬚菜在溫度與鹽度效應下生理活性反應................................14
3.1.1 藻體缺乏營養鹽狀態下成長適應測定.................................14
3.1.2 藻體蛋白質測定.................................................15
3.1.3 藻體游離胺基酸(free amino acid; FAA)測定.....................17
3.1.4 藻體內硝酸測定.................................................19
3.1.5 藻體硝酸還原酵素活性測定........................................20
3.2 龍鬚菜在各溫度與鹽度培養後餵食九孔其免疫抗病反應.....................21
3.2.1 九孔餵食各溫度與鹽度培養之龍鬚菜其PO反應..........................21
3.2.2 九孔餵食不同溫度與鹽度培養之龍鬚菜其O2-反應.......................28
第4章 討論..........................................................35
4.1 不同溫度與鹽度對大型藻類的影響.....................................35
4.2 龍鬚菜在不同溫度與鹽度下之生理活性.................................36
4.3 大型海藻多醣對免疫活性之影響......................................37
4.4 酚氧化酵素及超氧化離子之反應......................................38
第5章 結論..........................................................42
參考文獻............................................................43
作者簡介............................................................52
朱金錫、鍾虎雲、陳弘成、林恆雄,1990。台北縣九孔池水質與病害之初步研究。中國水產,451: 13-17。
江永錦,賴春福,1976。九孔之嗜食性研究。中國水產,284 : 6-7。
林明男,李棟樑,1960。龍鬚菜養殖法。台灣農業季刊,6:4。
桂德君,2004。環境因素對菊花心種龍鬚菜外表形態碎形維度的影響。國立中山大學海洋生物研究所碩士論文。
曾文陽,1985。鮑魚養殖學。前程出版社,pp. 191。
曾萬年,1976。台灣九孔貝生產生殖生態之研究。台灣水產學會刊,5(1): 24-32。
黃秋雁,1995。九孔。台灣農家要覽-漁業篇。豐年社,256-259。
張婉純,1998。龍鬚菜(Gracilaria tenuistipitata) (Gigartinales, Rhodophyta)高溫下游離輔胺酸(free proline)代謝。國立中山大學海洋生物研究所碩士論文。
莊豐銘,2002。鹽度對台灣鮑魚免疫反應之影響。國立屏東科技大學熱帶農業研究所碩士論文。
楊鴻禧、丁雲源,1989a。九孔陸上養殖池生產力之研究。台灣省水產試驗所試驗報告,46: 215-223。
楊鴻禧、丁雲源,1989b。九孔陸上養殖法專輯。台灣省水產試驗所試驗報告,1-36。
劉錦芬,2001。台灣龍鬚菜萃取物之免疫生理活性探討。國立台灣海洋大學食品科學系碩士論文。
Adachi, Y., Ohno, N., Ohsawa, M., Oikawa, S., Yadomae, T., 1990. Macrophage activation in vitro by chemically cross-linked (1,3)- β-D-glucans. Chem. Pharm. Bull. 38, 988-992.
Adema, C. M., van der Knaap, W. P. W., Sminia, T., 1991. Molluscan hemocytemediated cytotoxicity:the role of reactiveoxygenintermediates. Rev. Aquat. Sci. 4, 201-223.
Armisen, R., 1995. World-wide use and importance of Gracilaria. J. Appl. Phycol. 7, 231-243.
Asare, S. O., Harlin, M. M., 1983. Seasonal fluctuations in tissuenitrogen for five species of perennial macroalgae in Rhode Island Sound. J. Phycol. 19, 254-257.
Ashida, M., Yamazaki, H. I., 1990. Biochemistry of the phenoloxidase system in insects: with special reference to activation. In: Ohnishi, E. and M. Ishizaki(eds.), Molting and Metamorphosis. Sci. Soci.Tokyo: Japan, pp. 239-265.
Asokan, R., Arumugam, M., Mullainadhan, P., 1997. Activationofprophenoloxidase in the plasma and hemocytes of the marine mussel Perna viridis Linnaeus. Dev. Comp. Immunol. 21, 1-12.
Babior, B. M., Kipnes, R. S., Curnutte, J. T., 1973. The production byleukocytes of superoxide, a potential bactericidal agent. J. Clin. Invest. 52, 741-744.
Beer, S., Israel, L., 1983. Effects of photon fluence rate and light spectrum composition on growth, photosynthesis and pigment relations in Gracilaria sp. J. Phycol. 19, 516.522.
Bird, N. L., Chen, L. C. M., McLachlan, J., 1979. Effect oftemperature, light and salinity on growth in culture of Chondrus crispus, Furcellaria lumbricalis, Gracilaria tikvahiae (Gigartinales, Rhodophyta), and Fucus serratus (Fucales, Phaeophyta). Botanica Marina. 22, 521-527.
Bird, T. K., Benson, P. H., 1987. Seaweed Cultivation for Renewable Resources. Development in Aquaculture and Fisheries Science, 16, Elsevier, 200-201.
Blondin, C., Fischer, E., Boisson-Vidal, C., Kazatchkine, M. D., Jozefonvicz, J., 1994. Inhibition of complement activation by natural sulfatedpolysaccharides (fucoidans) from brown seaweed. Mol. Immunol. 31, 247-253.
Bradford, M. M., 1976. A rapid sensitive method for the quantitation ofmicrogram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
Brinkhuis, B. H., Williams, S. L., Tobin, S. 1986. Nitrogen and carbon profile in Laminaria saccharina(Phaeophyta)blade. Abstrct of the 25th Northeast Algal Symposium, April 19-20, 1986, Woods Hole, MA.
Brinkhuis, B. H., Wu, R., Li, C., Jiang, X. S., 1989. Nitrite uptake transient and consequence for in vivo algal nitrate reductase assays. J. Phycol. 25, 539-545.
Chen, J. C., Chen, W. C., 2000. Salinity tolerance of Haliotis diversicolor supertexta at different salinity and temperature levels. Aquaculture 181, 191-203.
Chen, J. C., Lee, W. C., 1999. Growth of Taiwan abalone Haliotis diversicolor supertexta fed on Gracilaria tenuistipitate and artificial diet in a multipletier basket system. J. Shellfish Res. 18, 627-635.
Chen, L. C., 1990. Aquaculture in Taiwan. Blackwell Scientific Publication Ltd. Great Britain, pp. 260-267.
Chen, H. C., Yang, H. H., 1979. Artificial propagation of the abalone Haliotis diversicolor supertexta. China Fish. Monthly. 314, 3-9.
Chen, J. H., 1996. Hemolymph collection in abalone(Haliotis diversicolor). Acta. Zool. Taiwan. 7, 61-71.
Chen, J. H., Yang, H. Y., Peng, S. W., Chen, Y. J., Tsai, K. Y., 1996. Characterization of abalone Haliotis diversicolor hemocytes in vitro. Biol. Bull. NTNU. 31(1), 31-38.
Church, F.C., Meade, J.B., Treanor, R.E., Whinna, H.C., 1989. Antitrombin activity of fucoidan: the interaction of fucoidan with heparin cofactor Ⅱ, antitrombin Ⅲ and trombin. J. Biol. Chem. 264, 3518-3623.
DeChatelet, L., Shirley, P. S., 1982. Chemiluminescence of human neutrophils induced by soluble stimuli:Effect of divalent cation. Infect. Immun. 35, 206-212.
Diamond, R. D., Clark, R. A., Haudenschild, C. C., 1980. Damage to Candida albicans Hyphae and pseudohyphae by the myeloperoxidase and oxidative products of neutrophil metabolism in-vitro. J. Clin. Invest. 66, 908-917.
Diamond, R. D., Kresicki, R., 1978. Mechanisms of attachment of neutrophils to Candida albicans pseudohyphae in the absence of serum, and of subsequent damage to pseudohyphae by microbicidal processes of netrophils in vitro. J. Clin. Invest. 61, 360-369.
Ekman, P., Yu, S., Pedersen, M. 1991. Effects of altered salinity, darkness and algal nutrient galactosidase activity and agar yield of cultivated Gracilaria sordida. Br. Phycol. J. 26, 123-131.
Elyakova, L.A., Zvyagintseva, T.N., 1974. A study of the laminarins of some far-eastern brown seaweeds. Carbohydr. Res. 34, 241-248.
Feng, S. Y., 1988. Cellular defense mechanisms of oyster and mussels. In: Fisher, W. S.(Ed.), Disease Processes in Marine Nivalve Molluscs. American fisheries society, Benthesda, Maryland, USA. 18, 153-168.
Fisher, W. S., 1986. Structure and function of oyster hemocytes. In: Brehelin, M.(Ed.), Immunity in invertebrates. Springr-Verlag, Berlin, Germany. pp. 25-35.
Fisher, W. S., Newell, R. I. E., 1986. Salinity effects on the activity of granular hemocytes of American oysters, Crassostrea virginica. Biol. Bull. Mar. Biol. Lab. Woods. Hole. 170(1), 122-134.
Fisher, W. S, Tamplin, M., 1988. Environmental influence on activities and foreign-particle binding by hemocytes of American oysters, Crassostrea virginica. Can. J. Fish. Aquat. Sci. 45(7), 1309-1315.
Foley, D. A., Cheng, T. C., 1975. A quantitative study of phagocytosis by hemolymph cells of the pelecypods Crassostrea virginica and Merecnaria merecenaria. J. Invertebr. Pathol. 25, 189-197.
Fridovich, I., 1978. The biology of oxygen radicals. Science. 201, 875-880.
Gao, Y., Smith, G. J., Alberte, R. S., 2000. Temperature dependence of nitrate reductase activity in marine phytoplankton: biochemical analysis and ecological implications. J. Phycol. 36, 304-313.
Hughes, H. P. A., 1988. Oxidative killing of intracellular parasites mediated by macrophages. Parasitol. Today 4, 12.
Hurtado-Ponce, A.Q., Umezaki, I., 1987. Growth rate studies of Gracilaria verrucosa (Gigartinales, Rhodophyta). Botanica Marina. 30, 223.226.
Ino, T., 1952. Biological studies on the propagation of Japanese abalone(genus Haliotis). Tokai Reg. Fish. Res. Lab. 102pp.
Jong, E. C., Mahmou, A. A. F., Klebanoff, S. J., 1981. Peroxidase mediated toxicity to schistosomula of Shistosoma mansoni. J. Immunol. 126, 468-471.
Kinne, O., 1963. The effect of temperature and salinity on marine and brackish water animals: I. Temperature. Oceanogr. Mar. Biol. Annu. Rev. 1, 301-340.
Klebanoff, S. J., Henderson, W. R., Jong, E. C., Jong, A., Locksley, R. M., Ramsey, P. G., 1982. Cidal mechanism and their dependence on hydrogen peroxide. In: Karnovsky, M. L., L. Bolis(Eds.), Phagocytosis-Past and Present. Academic Press, New York, pp. 449-461.
Kulicke, W. M., Lettau, A. I., Thielking, H., 1997. Correlation between immunological activity, molar mass and molecular structure of different (1→3)- β-D-glucans. Carbohydr. Res. 297, 135-143.
Lalli, C. M., Parsons, T. R. 1993. Biological Oceanography: An Introduction, Pergamon Press, oxford, UK. 295pp.
Lapointe, B. E., Duke, C. S. 1984. Biochemical strategies for growth of Gracilaria tikvahiae (Rhodphyta) in relation to light intensity and nitrogen availability. J. phycol. 20, 488-495.
Lee, T. M., Chang, Y. C., Lin, Y. H., 1999. Differences in physiological responses between winter and summer Gracilaria tenuistipitata (Gigartinales, Rhodophyta) to varying temperature. Bot. Bull. Acad. Sin. 49, 93-100.
Lionel, C., Alain, F., Frederic, C., Nelly, K., Corinne, S., Anne-Marie, F., Catherine, B., 1999. Further data on the structure of brown seaweed fucans: relationships with anticoagulation activity. Carbohydr. Res. 319, 154-165.
Lobban, C. S., Harrison, P. J. 1994. Seaweed ecology and physiology, Cambridge University Pree, New York. 366pp.
Lüning, K. 1990. Seaweeds-Their Environment, Biogeopraphy, and Ecophysiology, John wiley and Sons Inc, New York. 527pp.
McGlatjry, K. J. 1996. Changes in intracellular nitrogen pools and feedback control on nitrogen uptake in Chaetomorpha linum(chlorophyta). J. Phycol. 32, 393-401.
Meadows, P. S., Campbell, J. I. 1993. An Introduction to Marine Science, Blackie Academic and Professional, Glasgow, UK. 285pp.
Momma, H., Sato, R., 1969. The locomotion behavior of the disc abalone Haliotis discus hannai Ino, in a tank. Tohoku J. Agri. Res. 21, 20-28.
Nathan, C., Nogueria, N., Juangbhanich, C., Ellis, J., Cohn, Z., 1979. Activation of macrophages in vivo and in vitro. Correlation betweem hydrogen peroxide release and killing of Trypanosoma cruzi. J. Exp. Med. 149, 1056.
Parson, T. R., Maita, Y., Lalli, C. M., 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford, 173pp.
Paul, B., Sbarra, A. S., 1986. The role of phagocyte in host-parasite interaction. XIII. The direct quantitative measurement of H2O2 in phagocytizing cells. Biochem. Biophys. Perspect. Mar. Biol. 156, 168-178.
Poore, G. C. B., 1972. Ecology of New Zealand abalone, Haliotis species (mollusca: gastropoda)Ⅱ. Seasonal and diurnal movement. New Zealand J. Mar. Freshwat. Res. 6, 246-258.
Ratcliffe, N. A., Rowley, A. F., Fitzgerald, S., Rhodes, W. C. P., 1985. Invertebrate immunity: basic concepts and recent advances. Int. Rev. Cytol. 97, 183-350.
Rodriguez, J., Moullac, G. L., 2000. State of the art of immunological tools and health control of prnaeid shrimp. Aquaculture. 191, 109-119.
Root, R. K., Metcalf, J. A., 1977. Relationship to superoxide anion formation and cellular catabolism H2O2:studies with normal and cytochalasin b-treated cells. J. Clin. Invest. 60, 1266-1279.
Root, R. K., Metcalf, J. A., Oshino, N. and Chance, B., 1975. H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation, and some regulating factors. J. Clin. Invest. 55, 945-955.
Shepherd, S. A., 1973. Studies on southern Australian abalone(genus Haliotis)I. Ecology of five sympatric species. Aust. J. Mar. Freshwat. Res. 24, 217.257
Smit, A. J., Robertson, B. L., Du Preez, D. R., 1997. Influence of ammonium-N pulse concentrations and frequency, tank condition and nitrogen starvation on growth rate and biochemical composition of Gracilaria gracilis. J. Appl. Phycol. 8, 473-481.
Söderhäll, K., Cerenius, L., 1998. Role of prophenoloxidase activating system in invertebrate immunity. Curr. Opin. Immunol. 10, 23-28.
Song, Y. L., Hsieh, Y. T., 1994. Immunostimulation of tiger shrimp (Penaeus monodon)cytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev. Comp. Immunol. 18, 201-209.
Surresh, K., Mohandas, A., 1990. Number and types of hemocytes in Sunetta scripta and Villorita cyprinodes var. cochinensis(Bivalve), and leukocytosis subsequest to bacterial challenge. J. Invertebr. Pathol. 55, 312-318.
Tatiana, N. Z., Natalia M. S., Irina, V. N., Andrey, S. S., Pavel, A. L., Ludmila, A. E., 2000. Inhibition of complement activation by water-soluble polysaccharides of some far-eastern brown seaweeds. Comparative Biochemistry and Physiology, Part C 126, 209-215.
Thorne, K. J. I., Svvennsen, R. J., Franks, D., 1978. Role of hydrogen peroxide and peroxidase in the cytotoxicity of Trypanosma dionisii by human granulocytes. Infect. Immun. 21, 798-805.
Tzeng, W. N., 1977. Studies on the growth and rearing environment of the abalone, Haliotis diversicolor supertexta Lischke. China Fish Month. 292, 2-7.
Winston, G. W., Moore, M. N., Kirchin, M. A., Soverchia, C., 1996. Production of reactive oxygen species by hemocytes from the marine mussel, Mytilus edulis: lysosomal localization and effect of xenobiotics. Comp. Biochem. Physiol. 113(2), 221-229.
Yu, S., Pedersen, M., 1990. The effect of salinity changes on the activity of alpha-galactosidase of the red algae Gracilaria sordida and G. tenuistipitata. Botanica Marina 33, 385-391.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top