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研究生:徐東儀
研究生(外文):Hsu-Tung Yi
論文名稱:淡水長臂大蝦感染酵母菌之生理反應
論文名稱(外文):Physiological Respones of Yeast (Debaryomyces hensenii)Infected Giant Freshwater Prawn, Macrobrachium rosenbergii.
指導教授:鄭文騰鄭文騰引用關係
指導教授(外文):Winton Cheng
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:水產養殖系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:76
中文關鍵詞:淡水長臂大蝦酵母菌滲透壓離子調節酸鹼平衡葡萄糖肝醣
外文關鍵詞:Macrobrachium rosengergiiDebaromyces hanseniiosmolalityionsacid-base balanceglucoseglycogen
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本研究做為評估淡水長臂大蝦(Macrobrachium rosenbergii)被酵母菌(Debaryomyces hansenii)感染的生理代謝因子的變化。探討淡水長臂大蝦注射3×105 cfu/ml之酵母菌,分析其致病濃度及對於血淋巴生理指標之影響,包括:血淋巴滲透壓及鈉離子、鉀離子、氯離子、鈣離子、鎂離子之變動、酸鹼平衡及氣體分壓之變動、葡萄糖及乳酸之變動、肝胰腺及肌肉中肝醣濃度之變動等。血淋巴中酵母菌濃度高達109 cfu/ml時,K+濃度、pH值、二氧化碳分壓(pCO2)、肝胰臟之肝醣含量等生理指標沒有顯著影響;血淋巴中酵母菌濃度為105 cfu/ml時,滲透壓及Na+濃度、Cl-濃度出現顯著性下降;血淋巴中類酵母菌濃度為109 cfu/ml、107 cfu/ml時,Ca2+濃度、Mg2+濃度分別出現明顯的上升;淡水長臂大蝦感染酵母菌濃度為103 cfu/ml時,血淋巴之乳酸、葡萄糖及氨濃度均出現明顯的上升。淡水長臂大蝦感染酵母菌濃度高於104 cfu/ml時,氧分壓(pO2)呈顯著下降;血淋巴中酵母菌濃度高於109 cfu/ml,肌肉中肝醣的含量會出現明顯的上升趨勢。這些結果證實了淡水長臂大蝦感染類酵母菌後,造成器官受損,因而導致生理代謝調節功能失調。
This present study profiles the physiological metabolism of giant freshwater prawn (Macrobrachium rosenbergii) infected with yeast (Debaromyces hansenii). The relationship between the concentration of yeast in prawn hemolymph and physiological parameters, which including hemolymph osmolality, sodium, potassium, chloride, calcium, magnesium, ammonia, glucose, lactate, partial pressure of oxygen (pO2), partial pressure of carbon dioxide (pCO2), acid-base balance and the concentration of glycogen in muscle and hepatopancreas, were analyzed after prawn were injected with yeast at 3x105 cfu/ml. Hemolymph potassium, pH, pCO2 and glycogen of hepatocancreas content of prawn were no significantly different while the concentration of yeast in hemolymph were up to109 cfu/ml. The hemolymph osmolality, sodium and chloride of prawn were significantly decreased while the concentration of yeast in hemolymph was higher than 105 cfu/ml. The hemolymph calcium and magnesium of prawn were significant increased while the concentration of yeast in hemolymph were 109 cfu/ml and 107 cfu/ml, respectively. The hemolymph glucose and lactate content of prawn were significant increase while the prawn infected with yeast at more than 103 cfu/ml. The concentration of hemolymph ammonia was significantly increased following prawn infected with yeast at more than 103 cfu/ml. The hemolymph pO2 of prawn was decreased significantly while prawn infected with yeast at more than 104 cfu/ml. The glycogen of muscle content of prawn was significantly increased while the concentration of yeast in hemolymph was more than 109 cfu/ml. The regulation physiological metabolism of prawn was influenced after prawns infected with yeast due to some organs of prawn were damaged.
目錄

摘要………………………………………………………………………. I
Abstract…………………………………………………………………… II
誌謝………………………………………………………………………. IV
目錄 ……………………………………………………………………. Ⅴ
圖表目錄…………………………………………………………………. VIII
第1章 前言……………………………………………………………. 1
第2章 文獻探討………………………………………………………. 5
2.1 淡水長臂大蝦分類地位、分布及生態習性…………………….. 5
2.2 台灣淡水長臂大蝦養殖沿革與現況…………………………….. 6
2.3 淡水長臂大蝦常見的疾病……………………………………….. 7
2.4 酵母菌之分類地位…………….…………………………………. 10
2.5 酵母菌之分離及鑑定方法……………..………………………… 11
2.6 甲殼類生理調節機制……………………………………………. 12
2.6.1 滲透壓及離子調節………………………………………….. 12
2.6.2 酸鹼平衡…………………………………………………….. 13
2.6.3 肝醣、葡萄糖及乳酸代謝………………………………… 14
2.6.4 氨(NH3)代謝…………………………………………….. 15
2.7 Debaryomyces hansenii對甲殼類生理調節機制的影響……… 17
第3章 材料與方法……………………………………………………. 20
3.1 試驗設計……………………………………………………….…. 20
3.2 實驗用水……………………………………………………….…. 20
3.3 實驗用蝦………………………………………………………….. 20
3.4 試驗用試液的製備.………………………………………………. 20
3.5 病原菌的製備…………………………………………………….. 21
3.5.1淡水長臂大蝦血淋巴病原菌濃度的測定…………………… 21
3.6 Debaromyces hansenii對淡水長臂大蝦血淋巴生理因子分析...... 21
3.6.1血淋巴滲透壓分析…………………………………………… 21
3.6.2血淋巴Na+、K+及Cl-分析…………………………………. 21
3.6.3血淋巴Ca2+分析……………………………………………… 22
3.6.4血淋巴Mg2+離子分析……………………….........................… 22
3.6.5酸-鹼平衡測定.……………………………………………….. 22
3.6.6乳酸和葡萄糖測定.…………………………………………… 23
3.6.6.1對血淋巴之影響測定………………………………….… 23
3.6.6.2血淋巴乳酸含量之測定……………………………….… 23
3.6.6.3血淋巴葡萄糖含量之測定…………………………….…. 24
3.6.7組織中肝醣之測定.…………………………………………… 24
3.6.8 NH3的測定.………………………………………….………. 25
3.6.9統計分析.……………………………………………………… 25
第4章 結果……………………………………………………………. 26
4.1 D. hansenii對淡水長臂大蝦滲透壓之影響……………………… 26
4.2 D. hansenii對淡水長臂大蝦Na+、K+及Cl-調節之影響……… 27
4.3 D. hansenii對淡水長臂大蝦Ca2+、 Mg2+調節之影響………… 27
4.4 D. hansenii 對淡水長臂大蝦血淋巴酸-鹼平衡之影響…………. 30
4.5 D. hansenii對淡水長臂大蝦血淋巴葡萄糖及乳酸含量之影響………………………………………………………………... 32
4.6 D. hansenii對淡水長臂大蝦血淋巴及肝胰臟組織中肝醣含量之影響………………………………………………………... 34
4.7 D. hansenii 對淡水長臂大蝦血淋巴NH3之影響.…………….…. 35
第5章 討論……………………………………………………………. 36
5.1內外因子對D. hansenii感染淡水長臂大蝦之影響……………… 36
5.2 D. hansenii對淡水長臂大蝦滲透壓、Na+、K+及Cl-、Ca2+及Mg2+調節之影響………………………………………….. 37
5.3 D. hansenii 對淡水長臂大蝦血淋巴酸-鹼平衡之影響.………… 40
5.4 D. hansenii對淡水長臂大蝦血淋巴葡萄糖、乳酸及組織中肝醣含量之影響…………………………………………….….. 41
5.5 D. hansenii 對淡水長臂大蝦血淋巴NH3之影響………………. 43
第6章 結論……………………………………………………………. 45
參考文獻…………………………………………………………………. 46
附錄一……………………………………………………………………. 73
附錄二……………………………………………………………………. 74
作者簡介…………………………………………………………………. 76
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