臺灣博碩士論文加值系統

本論文以丁香、山茱萸、山梔子、杏仁、防己、防風、黃芩、黃連、橘紅及龍膽等10種藥材及葛根湯、小青龍湯、桂枝湯、麻杏甘石湯及黃連解毒湯等5個方劑為研究對象，開發高效液相層析(HPLC)方法。
在藥材方面，丁香以eugenol、山茱萸以loganin、山梔子以geniposide 、杏仁以amygdalin 、防己以tetrandrine、防風以5-O-methylvisamminol為指標成分，黃芩以baicalin, baicalein, wogonin、黃連以berberine, coptisine, palmatine, berberastine、橘紅以hesperidin, nobiletin, tangeretin、龍膽以gentiopicroside, swertiamarin等為指標成分，開發各藥材之HPLC分析方法。
在方劑方面，葛根湯以葛根(puerarin，daidzin，daidzein)、桂枝(cinnamic acid)、白芍(paeoniflorin)、炙甘草(glycyrrhizin)及生薑(gingerol)等7種指標成分，小青龍湯以白芍(paeoniflorin，paeonol)、桂枝(cinnamic acid，cinnamaldehyde)、炙甘草(glycyrrhizin)、五味子(gomisin A，schizandrin)、乾薑(gingerol)等8種指標成分，桂枝湯以白芍(paeoniflorin，benzoic acid，paeonol)、桂枝(cinnamic acid，cinnamaldehyde)、炙甘草(glycyrrhizin)等6種指標成分，黃連解毒湯以黃連(coptisine，palmatine)、黃柏(palmatine)、黃芩(baicalin，baicalein，wogonin)、山梔子(geniposide) 等6種指標成分，麻杏甘石湯以杏仁(amygdalin)、炙甘草(glycyrrhizin)等2種指標成分，開發上述5種方劑多成分同時分析之HPLC分析方法。
上述10種藥材及5個方劑之HPLC分析方法皆經確效試驗之驗證，得到安定且值得信賴之定量法，建立檢驗標準，達到品質管制之目的。

Ten Chinese herbal medicines and five Chinese medicine preparations, including Caryophylli Flos, Corni Fructus, Gardeniae Fructus, Armeniacae Semen, Aristolochiae Fangchi Radix, Ledebouriellae Radix, Scutellariae Radix, Coptidis Rhizoma, Citri Exocarpium Rubrum, Gentianae Scabrae Radix, Ko-Keng-Tang, Hsiao-Ching-Lung-Tang, Kuei-Chih-Tang, Ma-Hsing- Kan-Shih-Tang, and Huang-Lien-Chieh-Tu-Tang were selected for model drugs to develop analytic HPLC techniques.
In the Chinese herbal medicine, eugenol in Caryophylli Flos, loganin in Corni Fructus, geniposide in Rardeniae Fructus, amygdalin in Armeniacae Semen, tetrandrine in Aristolochiae Fangchi Radix, 5-O-methylvisamminol in Ledebouriellae Radix, baicalin, baicalein, wogonin in Scutellariae Radix, berberine, coptisine, palmatine, berberastine in Coptidis Rhizoma, hesperidin, nobiletin, tangeretin in Citri Exocarpium Rubrum, gentiopicroside, swertiamarin in Gentianae Scabrae Radix were used as indicator components to analyze multi-components simultaneously by HPLC.
As for the five Chinese medicine preparation, we used these five preparations to analyze multi-components simultaneously by HPLC. Ko-Keng-Tang which includes puerarin, daidzin and daidzein in Puerariae Radix, cinnamic acid in Cinnamomi Ramulus, paeoniflorin in Paeoniae Radix, glycyrrhizin in Glycyrrhizae Radix, and gingerol in Zingiberis Rhizoma. In Hsiao-Ching-Lung-Tang includes paeoniflorin and paeonol in Paeoniae Radix, cinnamic acid and cinnamaldehyde in Cinnamomi Ramulus, glycyrrhizin in Glycyrrhizae Radix, gomisin A and schizandrin from Schizandrae Fructus, and gingerol in Zingiberis Rhizoma. In Kuei-Chih-Tang includes paeoniflorin, benzoic acid and paeonol in Paeoniae Radix, cinnamic acid and cinnamaldehyde in Cinnamomi Ramulus, glycyrrhizin in Glycyrrhizae Radix. In Huang-Lien-Chieh-Tu -Tang includes coptisine and palmatine in Coptidis Rhizoma, palmatine in Phellodendri Cortex, baicalin, baicalein and wogonin in Scutellariae Radix, and geniposide in Gardeniae Fructus, and Ma-Hsing-Kan-Shih-Tang includes amygdalin in Armeniacae Semen, and glycyrrhizin in Glycyrrhizae Radix.
The analytic HPLC methods described above have been confirmed to be reliable for quantification. These methods can also be used to establish standards for quality control to ensure the accuracy and efficiency in Chinese herbal medicine and preparations.

摘 要 I
Abstract III
誌 謝 V
目 錄 VI
圖表目錄 VII

壹、前言 1
貳、文獻回顧 3
一、丁香、山茱萸、山梔子、杏仁、防己、防風、 黃芩、黃連、橘紅及龍膽等10種中藥材簡述 3
二、葛根湯、小青龍湯、桂枝湯、麻杏甘石湯 及黃連解毒湯等五種方劑 9
三、層析法及高效能液相層析儀法(HPLC) 11
參、材料與方法 14
一、材料 14
二、方法 16
肆、結果 40
一、藥材的外觀及粉末特徵 40
二、10種藥材成分定量分析 41
三、5種方劑成分定量分析 83
伍、討論 117
一、單一藥材特徵與成分變化 117
二、葛根湯、小青龍湯、桂枝湯、麻杏甘石湯及 黃連解毒湯成分分析 123
陸、結論 125
參考文獻 127
附 錄 140
作者簡介 146

王軍、張寶善、陳錦屏 (2003) 紅棗營養成分及其功能的研究，食品研究與開發24：68-72。
王志堯 (1999) 小青龍湯對於過敏氣喘的免疫調控及免疫發炎反應的影響，行政院衛生署中醫藥年報18：47-64。
中藥檢驗方法專輯(九)-中藥濃縮製劑指標成分定量方法 (1996) 藥物食品叢書出版社，台北，台灣。
中華中藥典編修委員會 (2004) 中華中藥典，衛生署，台北，台灣。
台灣必安研究所 (2005) 防己，順天堂藥廠股份有限公司5：56-62。
沈紅 (1995) 感冒仍推葛根湯，東洋醫學23：49。
宋振民、周德剛、張建軍 (2006) 複方甘草酸苷(美能)治療活動性肝炎肝硬化的療效觀察，中華名醫論壇4：32-33。
宋曦 (2005) 從陰陽的角度認識桂枝湯為群方之魁，遼寧中醫雜誌32：453。
李水福 (1993) 中藥材品質管制之研究(3)：梔子指標成分-Geniposide台灣民間肝炎藥材之開發：葉下珠(2)，台北醫學院藥學研究所碩士論文。
李忠紅、聶晶、倪坤儀、杜冠華 (2005) 不同產地半夏的化學成分分析及比較，分析科學學報21：393-395。
李清、王璐、戴榮華、畢開順 (2004) 高效液相色譜法測定黃連解毒湯中鹽酸小檗鹼的含量，西北藥學雜誌19：51-52。
李國信、于豔、田原、劉桂芳 (2005) Rp-HPLC 法測定細辛根中馬兜鈴酸A的含量，遼寧中醫雜誌32：1080-1081。
林東煥 (2002) 以HPLC同步分析食品中五種防腐劑及兩種人工甜味劑，朝陽科技大學應用化學系碩士論文。
林敬二、林宗義 (1994) 儀器分析(下冊)，美亞書版股份有限公司，台北，台灣。
科技產業現況與市場趨勢研討會 (2001) 生物技術及製藥產業、食品產業，台北，台灣。
洪秀珍 (1999) 葛根湯新用，陝西中醫學院學報22：18。
胡華勇、樂長高、梁華正 (2005) 梔子中京尼平甙的分離與含量測定，東華理工學院學報28：270-273。
徐士蘭、謝淑鐘、謝昀志、王慧瑛 (2002) 黃芩苷元對內皮細胞之生物反應，勝昌藥誌雜誌社34：161。
徐丹春 (2006) 桂枝湯新用，新中醫38：70。馬海琴、王志清 (2005) 甘草的開發與利用，特種經濟動植物10：22-23。
徐曉暉、趙磊、吳福祥、劉本亮 (2005) HPLC法測定黃柏膠囊中鹽酸小檗鹼的含量22：43-44。
財團法人製藥工業技術發展中心 (1995) 高效液相層析儀(HPLC)、氣相層析儀(GC)之應用與實習講義，台北，台灣。
高尚德 (1998) 麻杏甘石湯及其減方對過敏激發無麻醉天竺鼠立即性與遲發性反應呼吸阻力與發炎細胞影響，行政院衛生署中醫藥年報，16：273-295。
馬斌、任冬梅、趙焰、婁紅祥 (2002) 葛根湯口服液的薄層色譜鑑別。山東大學學報40：446-447。孫逸民、陳玉舜、趙敏勳、謝明學、劉興鑑 (2004) 儀器分析，權威圖書公司，台北，台灣。
許清祥、徐昀耀、李明憲 (2000) 中醫平喘方劑對於過敏原特異性呼吸道發炎反應的作用機轉評估，中國醫藥雜誌11: 222-121。
黃山、高紅寧、郭立瑋 (2006) 膜分離與樹脂聯用製備黃連解毒湯中藥固體製劑的研究，南京大學學報22：42-43。
張永勳 (2006) 封面介紹(麻黃)，名通醫藥349：1-1。
張永勳 (2005) 封面介紹(黃柏)，名通醫藥348：1-1。
張光雄 (1994) 中藥方劑之定性與定量。明通醫藥雜誌社。
張宏波、洪波 (2001) 乙醇回流萃取丁香酚的新方法，吉林大學自然科學學報2：106-108。
張朝棟 (1995) 麻杏甘石湯對家塵蟎致敏氣喘天竺鼠立即性與遲發性反應呼吸道阻力及發炎細胞之研究，中國醫藥學院中國醫學研究所碩士論文。
張賢哲、蔡貴花 (1992) 中藥炮製學，中國醫藥學院印行，台中，台灣。
張楠、王正、趙珊、羅人才 (2005) 保健食品中梔子中京尼平甙的測定，中國衛生檢驗雜誌15：1446-1447。
張儷俊 (2004) 小青龍湯治療小兒溢飲涕淚如湧泉之校驗報告，中醫藥研究論叢P.45。
勝昌藥誌編輯委員會 (2004) 封面藥用植物說明(山茱萸)，勝昌藥誌雜誌社36：7-10。
猶衛、王淑華 (2005) HPLC法測定複方甘草片中甘草苷的質量分數，寧夏工程技術4：354-355。
楊云、弓建紅、馮衛生、馬相斌 (2005) 大棗中多糖的化學研究，時針國醫國藥16：1215-1216。
楊世平、孫潤廣、陳國良、徐傳陽、王小勇 (2004) 紅棗中營養、藥用有效成分多醣的分離與提純及其鑑定，延安大學學報23：38-40。
楊玲玲、羅亞寧、陳立耿 (1999) 防風cimifugin之製備與HPLC品質管制，藥物食品分析7：191-197。
莊美淑、徐雅慧、張憲昌 (2001) 市售防風類藥材之鑑定，藥物食品檢驗局調查研究年報19：126-137。
莊武璋、李明宗、陳奎福、許順吉、張永賢 (2003) 加味逍遙散製劑之品質評價，中國醫藥雜誌14:183-191。
趙永娟、王蕾、侯琳、張向農、吉中強、岳旺 (2006) 半夏多糖抗腫瘤作用研究，中國藥理學通報22：368-371。
趙永席、馬國營、梁恒 (2006) 中藥山茱萸的高效液相色譜指紋圖譜及聚類分析，西安交通大學學報40：239-242。
雷鵬，劉韶、李新中、付文基 (2005) 黃連解毒湯傳統湯劑與配方顆粒湯劑主要活性成分含量比較，中藥新藥與臨床藥理16：353-356。
萬建麟 (2002) 柑橘類果皮成分分析及品質之研究，國立屏東科技大學熱帶農業研究所碩士論文。
葛爾寧 (2005) RP-HPLC法測定葛根湯中葛根素的的含量及變化，中國實驗方劑學雜誌11：12-13。
葉政洳 (2000) 黃芩成分之分析與基原之辨識，國立台灣師範大學化學研究所碩士論文。
鄧正賢、顧佑瑞、張永勳 (2002) 黃芩苷元對內皮細胞之生物反應，勝昌藥誌雜誌社34：161-162。
劉媛、楊文革、趙秀芳、陳昭文、包意康 (1996) 葛根湯的藥理研究及臨床應用18：38-39。
臧堃堂 (2005) 中藥材輕百科，山岳文化圖書有限公司，台北，台灣。
戴冰、王誠元、鄧雙華、劉磊、范新科 (2006) 不同來源山茱萸中馬錢苷含量的測定。湖南中醫學院學報26：21-22。
蔡東湖、洪傳岳、陳介甫 (1997) 以高效液相層析儀及相關技術中草藥的藥效成分，藥物食品分析5：303-318。
謝萬宗 (1959) 中藥材品種論述(中冊)，上海科學技術出版社，上海，中國。
顏焜熒 (1992) 原色生藥學。南天書局，台北，台灣。
竇紅霞、丁一方、劉愛華、李豔慧 (2005) 桂枝湯的現代研究進展，中醫藥信息22：52-53。
顧祐瑞、劉宜祝、郝景平、溫國慶、林哲輝、黃文鴻 (1995) 天麻藥材中指標成分Parishin, Parishin B及C之高效液相層析定量法之探討。藥物食品分析 3：287-294。
蘇淑于 (2005) 中藥材板藍根及大青葉之成分分析與基原辨識，國立台灣師範大學化學研究所碩士論文。
日本分析化學會北海道支部 (1989) 新分析化學實驗，(株)化學同仁，京都，日本。
原田正敏 (1989) 繁用生藥的成分定量，廣川書店，東京，日本。
Calis, I., M. F. Lahloub, and O. Sticher (2004) Loganin, loganic acid and periclymenoside, a new biosidic ester iridoid glucoside from Lonicera periclymenum L. Helvetica Chimica Acta 67: 160-165.
Campa, C., Ph. Schmitt-Kopplin, T. R. I. Cataldi, S. A. Bufo, D. Freitag, and A. Kettrup (2000) Analysis of cyanogenic glycosides by micellar capillary electrophoresis. J. Chromatogr. B 739: 95-100.
Chang, C. Y., M. Y. Lue, and T. M. Pan (2005) Determination of adenosine, coedycepin and ergosterol contents in cultivated Antrodia camphorata by HPLC method. J. Food Drug Anal. 13: 338-342.
Chang, L. C., and S. J. Sheu (1993) Quantitative Analysis of Cardamon and Fennel Combination. J. Food Drug Anal. 1: 183-189.
Chen, D. H., and W. K. D. Chen (2003) Determination of ganoderic acids in triterpenoid constituents of Ganoderma tsugae. J. Food Drug Anal. 11: 195-201.
Chen, G., H. W. Zhang, and J. N. Ye (2000) Determination of baicalein, baicalin and quercetin in Scutellariae Radix and its preparations by capillary electrophoresis with electrochemical detection. Talanta 53: 471-479.
Chen, Y. R., K. C. Wen, and G. R. Her (2000) Analysis of coptisine, berberine and palmatine in adulterated Chinese medicine by capillary electrophoresis-electrosprary ion trap mass spectrometry. J. Chromatog. A 866: 273-280.
Chen, Y. W., D. G. Li, J. X. Wu, Y. W. Chen, and H. M. Lu (2005) Tetrandrine inhibits activation of rat hepatic stellate cells stimulated by transforming growth factor-β in vitro via up-regulation of Smad 7. J. Ethnopharmacology 14: 299-305.
Chou, C. J., L. C. Lin, C. Peng, and Y. C. Chen (2002) Determination of tetrandrine, fangchinoline, cyclanoline and oblonging in radix stephanine tetrandrae by high-performance liquid chromatography. J. Chin. Med. 13: 39-48.
Gao, Z. H., K. X. Huang, X. L. Yang, and H. B. Xu (1999) Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochimica et Biophysica Acta 1472: 643-650.
Hamano, T., I Yasuda, N. Takahashi, I. Takano, T. Seto, Y. Watanabe, and K Akitama (1992) Pharmaceutical studies on Kampo Medicines (1). Simultaneous Determination of Morroniside, Loganin, Paeoniflorin and Paeonol in“Rokumi-gan”by High Performance Liquid Chromatography. Shoyakugaku Zasshi 47: 79-83.
Han, M. K., S. I. Kim, and Y. J. Ahn (2006) Insecticidal and antifeedant activities of medicinal plant extracts against Attagenus unicolor japonicus. J. Stored Products Research 42: 15-22.
Harada, M., Y. Ogihara, and Y Kano (1988) Quantitative Analysis of Chinese Pharmaceutical Preparations (Ⅰ). Iyakuhin Kenkyu 19: 852-860.
Harada, M., Y. Ogihara, and Y Kano (1989) Quantitative Analysis of Oriental Pharmaceutical Preparations (Ⅱ). Iyakuhin Kenkyu 20: 1300-1309.
Hayashi, H., G. Honda, M. Tabata, H. Yamamoto, E. Yesilada, and E. Sezik (1995) A survey of distribution and characteristics of Glycyrrhiza glabra L. in Turkey. Nat. Med. 49: 129-132.
He, X. G., M. W. Bernart, L. Z. Lian, and L. Z. Lin (1998) High-performance liquid chromatography-electrospray mass spectrometric analysis of pungent constituents of ginger. J. Chromatogr. A 796: 327-334.
Janbaz, K. H., and A. H. Gilani (2000) Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia 71: 25-33.
Jing, X., K. Akira, H. Hideki, and K. Fumiyo (2006) Determination of hesperidin in Pericarpium Citri Reticulatae by semi-micro HPLC with electrochemical detection. J. Pharm. Biomed. Anal. 21: 59-63.
Juang, L. J. and S. J. Sheu (2004) Chemical identification on the origins of Phellodendri Cortex. J. Food Drug Anal. 12: 46-51.
Kai, H., W. C. Chuang, and S. J. Sheu (1998) Separation of nine iridoids by capillary electrophoresis and high performance liquid chromatography. J. Chromatogr. A 803: 179-187.
Kakuda, R., T. Iijima, Y. Yaoita, K. Machida, and M. Kikuchi (2002) Triterpenoids from Gentiana scabra. Phytochemistry 59: 791-794.
Koo, J. Y., E. Y. Hwang, S. Cho, J. H. Lee, Y. M. Lee, and S. P. Hong (2005) Quantitative determination of maygdalin epimers from armeniacae semen by liquid chromatography. J. Chromatogr. B 814: 69-73.
Kumarasamy, Y., P. J. Cox, M. Jaspars, L. Nahar, and S. D. Sarker (2003) Cyanogenic glycosids from Prunus spinosa. Biochemical Systematics and Ecology 31: 1063-1065.
Lai, J. H. (2002) Immunomodulatory effects and mechanisms of plant alkaloid tetrandrine in antoimmune diseases. Acta Pharmacol. Sin. 23: 1093-1101.
Lai, M. Y., C. C. Chen, S. L. Hsiu, and P. D. Lee Chao (2001) Analysis and comparison of baicalin, baicalein, and wogonin contents in traditional decoctions and commercial extracts of Scutellariae Radix. J. Food Drug Anal. 9: 145-149.
Lay, H. L., C. C. Chen, S. C. Huang, T. M. Cham, and T. S. Wu (2005) Simultaneous analysis of ten components in patch preparation of Wan-Yin-Gau by high performance liquid chromatography. J. Food Drug Anal. 13: 118-124.
Lay, H. L., S. C. Huang, C. C. Chen, and T. S. Wu (2002) Studies on the component analysis and quality control in tonic wine preparation of King-Mon-Long-Fong-Jyo. J. Food Drug Anal. 11: 201-208.
Lay, H. L., C. C. Chen, and S. T. Chiang (2004) Simultaneous analysis of nine components in “Byi-Liang-Tang” preparation by high performance liquid chromatography. J. Food Drug Anal. 12: 115-119.
Lee, H. S., Y. E. Eom, and D. O. Eom (1999) Narrowbore high performance liquid chromatography of berberine and palmatine in crude drugs and pharmaceuticals with ion-pair extraction using cobalt thiocyanate reagent. J. Pharm. Biomed. Anal. 21: 59-63.
Lee, Y. C., C. Y. Huang, K. C. Wen, and T. T. Suen (1995) Determination of liquiritin, glycyrrhizin, hesperidin, cinnamic acid, cinnamaldehyde, honokiol and magnolol in the traditional Chinese medicinal preparation Wuu-Ji-San by high performance liquid chromatography. J. Chromatogr. A 692: 137-145.
Lei, V., W. K. A. Amoa-Awua, and L. Brimer (1999) Degradation of cyanogenic glycosides by Lactobacillus plantarum strains from spontaneous cassava fermentation and other microorganisms. International J. Food Microbiology 53: 169-184.
Li, Y., S. Cui, Y. Cheng, X. Chen, and Z. Hu (2004) Application of nonaqueous capillary electrophoresis for quantitative analysis of quinolizidine alkaloids in Chinese herbs. Analytica Chimica Acta 508: 17-22.
Lin, S. J., C. Y. Huang, K. C. Wen, and E. T. T. Suen (1994) Quantitative analysis of paeoniflorin, geniposide and glycyrrhizin in Jing-Jieh-Lian-Chyau-Tong by high performance liquid chromatography. J. Food Drug Anal. 2: 133-140.
Liu, S. L., L. Zhao, X. Liu, F. Zhou, and S. Jiang (2004) Simultaneous determination of bromhexine HCL and baicalin in Chinese compound medicine by a reversed-phase ion-pair HPLC. J. Food Drug Anal. 12: 306-310.
Mak, N. K., Y. L. Wong-Leung, S. C. Chan, J. M. Wen, K. N. Leung, and M. C. Fung (1996) Isolation of anti-leukemia compounds from citrus reticulata. Life Sciences 58: 1269-1276.
Nageswara Rao, R., and V. Nagaraju (2003) An overview of the recent trend in development of HPLC methods for determination of impurities in drugs. J. Pharm. Biomed. Anal. 33: 335-337.
Nout, M. J. R., G. Tunçel, and L. Brimer (1995) Microbial degradation of amygdalin of bitter apricot seeds. International J. Food Microbiology 24: 407-412.
Ohno, T., E. Mikami, H. Matsumoto, and N. Kawaguchi (2006) Identification tests of aristolochic acid in crude drugs by reversed-phase TLC/Scanning Densitometry. J. Health Sci. 52: 78-81.
Ortuño, A., I. Reynaldo, M. D. Fuster, J. Botía, D. G. Puig, F. Sabater, A. G. Lidón, I. Porras, and J. A. D. Río (1997) Citrus cultivars with high flavonoid contents in the fruits. Scientia Horticulturae 68: 231-236.
Pan, I. H., H. H. Chiu, C. H. Lu, L. T. Lee, and Y. K. Li (2002) Aqueous two-phase extraction as an effective tool for isolation of geniposide from gardenia fruit. J. Chromatogr. A 977: 239-246.
Peetermans, J. (1966) Factors affecting the stability of viral vaccine. Dev. Biol. Stand. 87: 97-101.
Peng, Q., Z. Wei, and B. H. S. Defense (1998) Fructus corni enhanes endothelial cell antioxidant defense. Gen. Pharmac. 31: 221-225.
Pourgholami, M. H., M. Kamalinejad, M. Javadi, S. Majzoob, and M. Sayyah (1999) Evaluation of the anticonvulsant activity of the essential oil of eugenia caryophyllata in male mice. J. Ethnopharmacology 64: 167-171.
Raman, G., G. K. Jayaprakasha, M. Cho, J. Brodbelt, and B. S. Patil (2005) Rapid adsorptive separation of citrus polymethoxylated flavones in non-aqueous conditions. Separation and Purification Technology 45: 147-152.
Rao, R. N., and V. Nagaraju (2003) An overview of the recent trends in development of HPLC methods for determination of impurities in drugs. J. Pharm. Biomed. Anal. 33: 335-377.
Shet, S. J., and C. F. Lu (1995) Determination of eight constituent of Hsiao-cheng-chi-tang by high performance liquid chromatography. J. Chromatogr. A 704: 518-523.
Sloley, B. D., S. R. Tawfik, K. A. Scherban and Y. K. Tam (2003) Quality control analyses for Ginkgo extracts require analysis of intact flavonol glycoside. J. Food Drug Anal. 11: 102-107.
Son, J., J. Lee, C. K. Han, W. J. Kwak, R. Yang, and D. H. Kim (2004) Simultaneous quantitation of loganin and sweroside in plasma using column-switching high- performance liquid chromatography. Analytica Chimica Acta 503: 257-262.
Sukumaran, K., and R. Kuttan (1995) Inhibition of tobacco-induced mutagenesis by eugenol and plant extracts. Mutation Research 343: 25-30.
Takano, I., N. Takahashi, T. Hamano, I. Yasuda, T. Seto, Y. Watanabe, and K. Akiyama (1994) Simultaneous Analysis of Various Components in Kampo Medicine“Un-Sei-In”(溫清飲) by High performance liquid chromatography. Natural Medicines 48: 111-115.
Teh, B. S., P. Chen, M. F. Lavin, W. K. Seow, and Y. H. Thong (1991) Demonstration of the induction of apoptosis (programmed cell death) by tetrandrine, a novel anti-inflammatory agent. Int. J. Immunopharmacol. 13: 1117–1126.
Tian, G., T. Zhang, F. Yang, and Y. Ito (2000) Separation of gallic acid from Cornus officinalis Sieb. et Zucc by high- speed counter-current chromatography. J. Chromatogr. A 886: 309-312.
Tirado, C. B., E. E. Stashenko, M. Y. Combariza, and J. R. Martinez (1995) Comparative study of Colombian citrus oils by high-resolution gas chromatography and gas chromatography-mass spectrometry. J. Chromatogr. A 697: 501-513.
Toshihiro, O. (2000) The protective effect of glycyrrhizin on anti-Fas antibody-induced hepatitis in mice. European J. Pharmacology 387: 229-232.
Tsai, P. L, and T. H. Tsai (2002) Simultaneous determination of berberine in rat blood, liver and bile using microdialysis coupled to high-performance liquid chromatography. J. Chromatog. A 961: 125-130.
Tsai, T. R., T. Y. Tseng, C. F. Chen, and T. H. Tsai (2002) Identification and determination of geniposide contained in Gardenia jasminoides and in two preparations of mixed traditional Chinese medicines. J. Chromatogr. A 961: 83-88.
Tuncel, G., M. J. R. Nout, and L. Brimer (1995) The effects of grinding, soaking and cooking on the degradation of amygdalin of bitter apricot seeds. Food Chemistry 53: 447-451.
Wang, S. F., X. G. Chen, Z. D. Hu, and Y. Ju (2003) Analysis of three effective components in Fructus corni and its preparations by micellar electrokinetic capillary chromatography. Bio. Chromatogr. 17: 306-311.
Wang, X., K. Saito, and Y. Kano (1993) On the Evaluation of the Preparation of Chinese Medicinal prescriptions (ⅤⅡ). HPLC Analysis of the Component in “Inchinko-to”(茵蔯蒿湯) and “ Inchin-gorei-san” (茵蔯五苓散). Shyoakugaku Zasshi 47: 243-248.
Wu, H. K., W. C. Chung, and S. J. Sheu (1998) Separation of nine iridoids by capillary electrophoresis and high-performance liquid chromatography. J. Chromatog. A 803: 179-187.
Yan, S. K., G. Luo, Y. Wang, and Y. Chen (2006) Simultaneous determination of nine components in Qingkailing injection by HPLC/ELSD/DAD and its application to the quality control. J. Pharm. Biomed. Anal. 40: 889-895.
Yoo, C. B., K. T. Han, K. S. Cho, J. Ha, H. J. Park, J. H. Nam, U. H. Kil, and K. T. Lee (2005) Eugenol isolated from the essential oil of eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells. Cancer Letters 225: 41-52.
Yoo, S. M., S. H. Oh, S. J. Lee, B. W. Lee, W. G. Ko, C. K. Moon, and B. H. Lee (2002) Inhibition of proliferation and induction of apoptosis by tetrandrine in HepG2 cells. J. Ethnopharmacology 81: 225-229.
Young, H. Y., Y. L. Luo, H. Y. Cheng, W. C. Hsieh, J. C. Liao, and W. H. Peng (2005) Analgesic and anti-inflammatory activities of [6]-gingerol. J. Ethnopharmacology 96: 207-210.
Zhou, T. T., G. R. Fan, Z. Y. Hong, Y. F. Chai, and Y. T. Wu (2005) Large-scale isolation and purification of geniposide from the fruit of Gardenia jasminoides Ellis by high-speed counter-current chromato- graphy. J. Chromatogr. A 1100: 76-80.