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研究生:陳俊璋
研究生(外文):Jiun-Jhang Chen
論文名稱:海鱺成長期間體脂肪細胞大小密度及脂肪生成相關參數之變化
論文名稱(外文):Changes in body adipocyte size and density as well as parameters relating to adipogenesis during the growth of the cobia Rachycentron canadum
指導教授:陳宏遠陳宏遠引用關係
指導教授(外文):Houng-Yung Chen
學位類別:碩士
校院名稱:國立中山大學
系所名稱:海洋生物研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:59
中文關鍵詞:海鱺細胞體積增大細胞生成脂肪酸合成酶
外文關鍵詞:cobiafatty acid synthasehyperplasiahypertrophy
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本研究探討箱網養殖海鱺 (cobia, Rachycentron canadum)年齡5∼31週間體脂肪組織的變化並分析細胞大小密度與脂肪生成相關生理參數之變化和關聯。自同批次箱網養殖海鱺於孵化後於5、7、9、13、17、21、26及31週分別採樣,分析肝臟脂肪酸合成酶 (Fatty acid synthase, FAS)、蘋果酸酶 (Mailc enzyme, ME)及葡萄糖-6-磷酸脫氫酶 (Glucose-6-phosphate dehydrogenase, G6PDH)活性與血清總三酸甘油酯、總膽固醇、磷脂質與游離脂肪酸濃度,並自肝臟、腹部肌肉與背部肌肉組織採樣分析脂肪細胞直徑分佈及細胞濃度。海鱺孵化後5週平均體重為13.6 ± 0.5 g,於中間育成場成長至87.5 ± 3.8 g後移入箱網,養殖至31週平均體重1690.8 ± 106.6 g。海鱺肝臟、腹部肌肉與背部肌肉脂肪含量皆隨養殖時間增長顯著增加,31週肝臟脂肪含量36.1 ± 3.2%、腹部肌肉脂肪含量14.8 ± 0.2%、背部肌肉脂肪含量4.6 ± 0.0%。海鱺肝臟脂肪酸合成酶活性無顯著變化;蘋果酸酶活性於海鱺移入箱網之後顯著增加;葡萄糖-6-磷酸脫氫酶活性隨成長顯著降低。血清總三酸甘油酯及磷脂質濃度隨養殖時間增長顯著增加,總膽固醇濃度則無顯著變化,游離脂肪酸濃度以9週及13週最高。肝臟脂肪細胞直徑隨海鱺箱網養殖時間增加而且有變小趨勢,肝臟脂肪細胞密度於13週顯著增加,17週後脂肪細胞密度維持在3070∼3356 x 104 cell/g tissue之間不再明顯變化。腹部肌肉脂肪細胞密度於13週顯著增加且隨養殖時間增長而增加,脂肪細胞平均直徑及中數無明顯趨勢變化。背部肌肉脂肪細胞密度亦於13週顯著增加,17週後脂肪細胞密度維持在255∼269 x 104 cell/g tissue之間不再顯著增加,脂肪細胞平均直徑及中數無明顯趨勢變化。由以上結果顯示:海鱺脂肪細胞在體重100∼400 g年齡7∼17週間大量生成 (hyperplasia),細胞變大 (hypertrophy)的現象在養殖至31週時並無出現,但中間育成階段未進入箱網養殖之前,肝臟脂肪細胞似有hypertrophy的現象。
This research investigated the changes in body adipocyte size and density as well as parameters relating to adipogenesis in growing cobia, Rachycentron canadum. The cobia from a same batch were sampled 5 , 7 , 9 , 13 , 17 , 21 , 26 and 31 weeks after hatching, Liver activities of fatty acid synthase, (FAS), mailc enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PDH) as well as serum total triacylglycerol (TG), total cholesterol (TC), phospholipid (PL), non-esterified fatty acid (NEFA) were assayed. Adipocyte abundance and size spectrum in liver, ventral muscle and dorsal muscle were determined. The cobia attained an average weight of 13.6 ± 0.5 g at week 5, and were cultured in nursery outdoor ponds until an average weight 87.5 ± 3.8 g when moved to open ocean cages. They grew to an average weight 1690.8 ± 106.6 g at week 31. The crude lipid content in liver, ventral and dorsal muscle increased significantly with fish age, and were 36.1 ± 3.2 g, 14.8 ± 0.2g, 4.6 ± 0.0 g at week 31. No significant change was found in hepatic FAS activity. Hepatic ME activity increased significantly after the fish were stocked in the cages. Whilr hepatic ME activity reduced with age, TG and PL were both increased significantly with age. While adipocyte diameter in liver showed a decreasing trend as the fish grew its abundance increased significantly at week 13 and maintained between 3070∼3356 x 104 cell/g tissue during week 17-31. Similarly, adipocyte abundance in ventral muscle increased significantly at week 13, and maintained between 255∼269 x 104 cell/g tissue afterwards. There was no significant change in size of ventral muscle adipocyte as fish grew. These results indicate that the adipocyte hyperplasia occurred during week 7- 13, when body weight was 100- 400 g. Adipocyte hypertrophy was observed when the fish reached week 31, there was a sign of adipocyte hypertrophy in liver when the fish were at the last phase of nursery outdoor ponds before being moved to the offshore cages.
目錄
中文摘要……………………………………………………………………………Ⅰ
英文摘要……………………………………………………………………………Ⅲ
目錄…………………………………………………………………………………Ⅳ
表目錄………………………………………………………………………………Ⅴ
圖目錄………………………………………………………………………………Ⅵ
文獻整理……………………………………………………………………………1
壹、前言……………………………………………………………………………8
貳、材料與方法……………………………………………………………………10
參、結果……………………………………………………………………………18
肆、討論……………………………………………………………………………38
伍、結論……………………………………………………………………………43
陸、參考文獻………………………………………………………………………44
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