臺灣博碩士論文加值系統

中 文 摘 要
目的：探討單次高強度運動誘發之氧化壓力對規律運動及/或長期吸菸者血液氧化指標及抗氧化酶活性的影響。方法：本研究分為控制組（C組）、運動組（E組）、吸菸組（S組）及吸菸加運動組（ES組）共四組，每組人數各為男性8人，共32人。實驗處理以跑步機實施85%VO2max的強度進行單次高強度運動至衰竭，並於運動前、後採集血液樣本分析血漿乳酸濃度、硫巴比妥酸反應物質 (TBARS) 濃度、氧化態低密度脂蛋白濃度 (oxLDL) 及紅血球溶胞液的過氧化氫酶 (CAT) 活性、麩胱甘肽過氧化酶 (GPx) 活性、超氧離子歧化酶 (SOD) 活性等。所得數據以獨立樣本 t 考驗、單因子變異數分析 (ANOVA) 與雙因子(運動 × 吸菸)共變數分析 (ANCOVA) 檢定。結果：除血漿乳酸濃度各組運動後皆顯著高於運動前 (p < .05) 外，其餘指標運動前後並無顯著差異。運動前各指標的比較如下：ES組GPx活性顯著高於E組 (p < .05)；E組、S組及ES組的SOD活性皆顯著高於C組 (p < .05)，且ES組亦顯著高於E組 (p < .05)；E組CAT活性稍高於C組，ES組亦稍高於S組 (惟未達顯著水準)；吸菸組（ES組及S組）血漿oxLDL顯著高於不吸菸組（C組及E組） (p < .05)，而血漿TBARS則無差異。結論：規律運動能增加紅血球抗氧化酶活性，而長期吸菸可能因加重氧化壓力而有加成效果，同時增加心血管疾病危險因子；且單次高強度運動並不會對吸菸者造成更高的氧化壓力。



關鍵詞：長期吸菸、規律運動、抗氧化酶

Abstract
Purpose: To investigate the effects of oxidative stress induced by one bout of high-intensity exercise on blood oxidative indicators and activities of antioxidative enzymes in subjects with regular exercise and/or with chronic cigarette smoking. Methods: There were four groups (8 subjects for each group) : control group (C group), exercise group (E group), smoking group (S group), and exercise-smoking group (ES group). Subjects performed one bout of high-intensity treadmill exercise ( 85％VO2max ) to volitional exhaustion. Blood samples were collected before and immediately after exercise to analyze plasma lactate, thiobarbituric acid reactive substances (TBARS), oxidized low density lipoprotein (oxLDL), and the activity of antioxidative enzymes in erythrocyte lysate, such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx). Paired t test, independent one way ANOVA and two-way (exercise × smoking) ANCOVA were used for statistical analysis. Results: Except for plasma lactate, which was significantly higher before exercise than after exercise (p < .05). There were no significant differences between time trials in all other indicators. The comparisons between groups were described as follows: GPx activity was significantly higher in ES group than in E group (p < .05), SOD activity was significantly higher in E group, S group and ES group in comparison with C group (p < .05), and was also significantly higher in ES group than in E group (p < .05). CAT activity was slightly higher in E group than in C group, and also in ES group than in S group, but not statistically. Plasma oxLDL in subjects with cigarette smoking (ES group and S group) was significantly higher than nonsmokers (C group and E group) (p < .05). However, plasma TBARS showed no significant differences between groups. Conclusion: These results indicated that regular exercise can increase the activity of antioxidative enzymes. However, chronic cigarette smoking may have an additive effect, aggravated with oxidative stress; and increase the risks of cardiovascular disease. In addition, one bout of high-intensity may not increase oxidative stress on smokers.




Keywords: chronic cigarette smoking, regular exercise, antioxidative enzymes

目 次

碩士論文通過簽名書…………………………………………… i
碩士論文授權書………………………………………………… ii
中文摘要………………………………………………………… iii
英文摘要………………………………………………………… iv
謝誌……………………………………………………………… v
目次……………………………………………………………… vi
表次……………………………………………………………… viii
圖次……………………………………………………………… ix
第壹章 緒論
一、研究背景………………………………………………… 1
二、研究目的………………………………………………… 3
三、研究假設………………………………………………… 3
四、名詞操作性定義………………………………………… 3
五、研究限制………………………………………………… 4
第貳章 相關文獻探討
一、氧化壓力………………………………………………… 5
二、吸煙與氧化壓力………………………………………… 7
三、運動與氧化壓力………………………………………… 8
四、本章總結………………………………………………… 11
第參章 研究方法
一、受試者…………………………………………………… 12
二、實驗設計………………………………………………… 12
三、實驗流程圖……………………………………………… 14
四、血液樣本分析…………………………………………… 15
五、統計分析………………………………………………… 17
第肆章 結果
一、受試者基本資料………………………………………… 18
二、乳酸……………………………………………………… 19
三、氧化指標………………………………………………… 20
四、抗氧化酶活性…………………………………………… 23
第伍章 討論與結論
一、單次高強度運動後血漿乳酸濃度的變化……………… 28
二、運動與吸菸對於氧化指標的影響……………………… 28
三、運動與吸菸對於抗氧化酶的影響……………………… 31
四、結論……………………………………………………… 33
引用文獻
一、中文部份………………………………………………… 34
二、西文部分………………………………………………… 35
附錄一 受試者須知…………………………………………… 40
附錄二 受試者參與同意書…………………………………… 41
附錄三 受試者基本資料及實驗相關調查表………………… 43
附錄四 受試者健康情況調查表……………………………… 44
附錄五 血液生化分析………………………………………… 45
個人小傳 51

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