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研究生:葉昭君
研究生(外文):Chao-Chun Yeh
論文名稱:人參皂苷對白蝦成長及免疫反應之影響
論文名稱(外文):The effects of ginsenosides on the growth and immunoreaction of white shrimp, Litopenaeus vannamei
指導教授:沈士新沈士新引用關係
指導教授(外文):Shyn-Shin Sheen
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:56
中文關鍵詞:白蝦人參皂苷成長免疫反應
外文關鍵詞:white shrimpLitopenaeus vannameiginsenosidesgrowthimmunoreaction
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利用中草藥添加於水產飼料,可視為代替化學合成藥物的新一代飼料添加劑。人參皂苷為人參之藥性有效成分,具有刺激細胞合成代謝、調節補體活性等功能。添加人參皂苷提取粉末具有藥性純度高及節省成本之優點,本論文於飼料中添加人參總皂苷粉末,探討人參總皂苷對白蝦成長及免疫之影響。
本論文分為三部分:(一)添加0~0.5%人參總皂苷提取粉末於基礎飼料中,探討人參皂苷對白蝦成長之影響。(二)添加0~0.25%人參總皂苷提取粉末於基礎飼料中,確立對白蝦成長最適之人參皂苷添加量。(三)以六組不同濃度之人參皂苷飼料投餵白蝦(0%、0.1%、0.2%、0.3%、0.4%、0.5%),於投餵後第七天取血液樣品分別進行免疫指標檢測。
實驗一以等蛋白、等能量之基礎飼料,七個處理組分別添加0%、0.05%、0.1%、0.2%、0.3%、0.4%和0.5%人參皂苷,每處理組三重複。實驗蝦體初重為0.14g,實驗期間每日餵食三次,任食至飽,為期六週。結果為添加0.2%人參皂苷組顯著優於控制組,其增重率達811.52%。
實驗二探討對白蝦成長最佳之人參皂苷添加量。以實驗一之基礎飼料添加0%、0.1%、0.15%、0.2%和0.25%人參皂苷,共五個處理組,每組三重複,實驗蝦體初重為0.0332g,實驗期間每日餵食三次,任食至飽,為期六週。添加0.2%人參皂苷組別有顯著高的增重率3532.8﹪。
實驗三探討人參皂苷對白蝦免疫反應之影響,六個處理組含0%、0.1%、0.2%、0.3%、0.4%和0.5%人參皂苷飼料投餵11.93±4.2 g白蝦,於投餵七天後,取血液樣品進行總血球數、血藍素、酚氧化酵素活性及超氧離子活性之檢測。總血球數方面,0.5%處理組顯著高於控制組、0.1%及0.2%處理組,其中又以控制組有最低值。攜氧血藍素含量各處理組間並無顯著差異。酚氧酵素活性方面,控制組顯著低於0.1%、0.2%、0.3%及0.5%處理組。超氧陰離子活性表現上,以控制組有顯著最高值,0.1%、0.2%及0.3%處理組有顯著低值。
本論文研究發現0.2%的人參總皂苷添加對於白蝦有最佳促進成長之效果;於免疫指標上,人參總皂苷可促進白蝦總血球數及酚氧酵素活性。
The levels of dietary ginsenosides for maximum weight gain and immune responses of white shrimp, Litopenaeus vanamei were determined. Seven isonitrogenous and isoenergetic semi-perified diets with ginsenosides levels range from 0 to 0.5% in 0.1% increments were evaluated in the first experiment. 0.14g white shrimp was fed three times a day to satiation. After 6 weeks experiment, weight gains of juvenile shrimp fed with diet containing 0.2%ginsenosides had significantly higher than those of shrimp fed with diet without ginsenosides in the first experiment. 0.033g juvenile shrimps was used in the second experiment. The weight gains of juvenile shrimp fed with diet containing 0.2% ginsenosides had significantly higher than those of shrimp fed the other treatment groups. L. vannamei fed with diets cntaining ginsenosides ranging from 0 to 0.5% in 0.1% increments were examined for the immune responses in the third experiment. No significant differentces in oxyhemocyanin amount among different treatments. There were a maximum total haemocyte count and phenoloxidase activity in 0.5% treatment, and significantly higher than the control group. The superoxidase anion production of L. vannamei fed the diet without ginsengosides was significantly higher than that of 0.1%、0.2%、0.3% and 0.5% shrimp fed the diets containing. This study suggested that 0.2% ginsanosides included in the diet can promote shrimp growth, and dietary ginsengosides can enhance the total haemocyte count and phenoloxidase activity of the shrimp. The mechanism of how ginsenosides affect on the white shrimp immunoreaction is needed for further research.
目錄
中文摘要..................................................Ⅰ
英文摘要..................................................Ⅲ
表目錄....................................................Ⅴ
前言.......................................................1
文獻整理...................................................4
一、白蝦...................................................4
二、人參皂苷...............................................6
三、植物皂素對水生生物之影響...............................9
四、甲殼類免疫機制........................................10
材料與方法................................................13
ㄧ、實驗動物..............................................13
二、實驗系統..............................................13
三、實驗條件..............................................13
四、飼料配置..............................................14
五、分析項目..............................................15
六、統計分析..............................................20
結果......................................................21
實驗一....................................................21
一、一般粗成份............................................21
二、初重、末重、增重率、存活..............................21
三、白蝦肌肉與肝胰臟的一般粗分析..........................22
四、飼料中人參皂苷含量分析................................22
實驗二....................................................23
一、一般粗成份............................................23
二、初重、末重、增重率、存活..............................23
三、白蝦肌肉與肝胰臟的一般粗分析..........................23
四、飼料中人參皂苷含量分析................................24
實驗三....................................................24
人參皂苷對白蝦免疫因子的影響..............................24
討論......................................................26
一、添加人參總皂苷對白蝦成長之影響........................26
二、添加人參總皂苷對白蝦免疫因子之影響....................29
參考文獻..................................................33
圖表......................................................41
附件......................................................52
附錄......................................................56
參考文獻
丁雲源,2004。白蝦養殖發展史。白蝦產業發展暨技術創新研討會-會議資料。
中山醫學院中藥臨床應用編寫組,1988。中藥臨床應用,啟業書局,262-264。
王庭富,孟正木,1999。人參皂甙Rg3對免疫功能的影響。中國藥科大學學報 30 (2), 133-135.
王密,許明榮,楊柯,2001。人參二醇組皂甙對游泳訓練大鼠抗氧化效應及免疫調節的研究。廣西醫科大學學報 18(4), 479-481.
吳淑惠,1999。使用高效率液體層析法和毛細管電泳法來分析人參和靈芝的有效成分。淡江大學化學系碩士班碩士論文。
宋增淦,2002。皂素、福馬林及地特松對淡水長臂大蝦血淋巴生理及免疫反應的影響。國立屏東科技大學熱帶農業研究所碩士學位論文。
李衛平,陳敏珠,1998。人參皂甙的免疫調節作用。安徽醫科大學學報 33 (5), 348-350.
胡松華,林鋒強,2003。人參皂甙Rb1 的免疫佐劑作用。中國獸醫學報 23 (5), 480-482.
張志純譯,1983。人參的科學研究。徐氏基金會,台北,33-35。
梁華平,王正國,耿波,1996。黃芪多糖及人參莖葉皂甙對創傷小鼠免疫器官影響。中醫研究 9(2), 15-17.
許明榮,王密,楊柯,2001。人參二醇組皂甙、三醇組皂甙對游泳訓練大鼠細胞免疫的影響。武漢體育學院學報 35(4), 48-52.
郭仁杰,2004。台灣白蝦養殖產業現況。白蝦產業發展暨技術創新研討會-會議資料。
陳國威,1994。皂素對班節蝦成長、酸鹼平衡及氮代謝之影響。國立台灣海洋大學水產養殖研究所碩士學位論文。
湯弘吉,1976。用茶粕殺除淡水長臂大蝦池中的害魚。中國水產 291, 2-4.
黃淑萍,2003。飼料中蛋白質來源及量與P/E比值對白蝦成長之影響。國立台灣海洋大學水產養殖研究所碩士學位論文。
楊淑媚,2004。人參皂苷Rh2抑制肺腺癌細胞生長及造成凋亡之分子作用機轉研究。中國醫藥大學中國醫學研究所碩士論文。
楊德群,2004。人參皂苷對腸道細胞吸收葡萄糖的作用與對肝癌細胞ATP含量的影響之研究。國防大學國防醫學院藥學研究所碩士論文。
廖一九,郭錦朱,王惠滿,戴慶玲,1989。福馬林及皂素對草蝦、斑節蝦、熊蝦、紅尾蝦、砂蝦和淡水長臂大蝦等蝦苗藥浴之忍受度試驗。魚病研究專集 9, 23-27.
劉俊達,王舒,劉紅濤,1994。中藥皂甙成分對老年人淋巴細胞功能的作用。中國病理生理雜誌 29(12), 717-721.
蔣豔,王毅,邱全瑛,2001。人參皂甙Rg1 的腸內菌代謝産物Rh1 對小鼠免疫細胞功能的影響。中國病理生理雜誌 17(8), 815.
儲岳峰,李祥瑞,胡元亮,2004。9 種中藥成分對小鼠免疫細胞活性的影響。南京農業大學學報 27(1), 97-100.
顏正華,1991。中藥學。知音出版社。734-736。



REFERENCES

Anderson, D.P., 1992. Immunostimulants, adjuvants and vaccine carriers in fish: application to aquaculture. Annual. Rev. Fish Dis. 2, 281-307.
AOAC, 1984. Official Methods of Analysis. 14th edition, AOAC, Arlington, VA, 1141 pp.
Bachére, E., Miallhe, E., Rodríguez, J., 1995. Identification of defence parameters in the haemolymph of crustacean with particular reference to the shrimp Penaeus japonicus(Bate), prospects and application. Fish Shellfish Immunol. 5, 597-612.
Bayne, C.J., 1990. Phagocytosis and non-self recognition in invertebrates. Phagocytosis appears to be an ancient line of defense. Bioscience 40, 723-731.
Boone, L., 1931. Anomuran crustacea from panama and canal zone. Bull. Ame. Mus. Natu. Histo. 63(2), 137-189.
Bureau, D.P., Harris, A.M., Cho, C.Y., 1998. The effects of purified alcohol extracts from soy products on feed intake and growth of chinook salmon (Oncorhynchus tshawyscha) and rainbow trout (Oncorhynchus mykiss). Aquaculture 161, 27-43.
Chen, J.-C., Chen, K.-W., Chen, J.-M., 1996. Effects of saponin on survival, growth, molting and feeding of Penaeus japonicus juveniles. Aquaculture 144, 165-175.
Danesi, R., Figg, W.D., Reed, E., Myers, C.E., 1995. Paclitaxel(taxol)inhibits protein isoprenylation and induces apoptosis in PC-3 human prostate cancer cells. Mol. Pharmacol. 47, 1106-1111.
Destoumieux, D., Bulet, P., Loew, D., Van Dorsselaer, A., Rodríguez, J., Bachère, E., 1997. Penaeidins: a new family of antimicrobial peptides in th shrimp, Penaeus vannamei (Decapod). J. Biol. Chem. 272, 28398-28406.
Enomoto, Y., Ito, K., Kawagoe, Y., Morio, Y., Yamasaki, Y., 1986. Positive inotropic action of isolated atrial and papillary muscles from the guinea-pig. Br. J. Pharmacol. 88, 431-450.
Ey, P.L., 1991. Phagocytosis in crustacean: The role of opsonins. In: Gupta, A.P. (Ed.), Immunology of insects and other arthropods. CRC press, Boca Raton, FL, pp. 201-235.
Folch, J., Lee, M., Stanely, C.H.S., 1957. A simple method for the isolation and purification total lipids from animal tissue. J. Biol. Chem. 226, 497-509.
Francis, G., Makkar, H.P.S., Becker, K., 2002. Dietary supplementation with a Quillaja saponin mixture improves growth performance and metabolic efficiency in common carp (Cyprinus carpio L.). Aquaculture 203, 311-320.
Freeland, W.J., Calcott, P.H., Anderson, L.R., 1985. Tannins and saponin: interaction in herbivore diet. Biochem. Syst. Ecol. 13(2), 189-193.
Ghiretti-Magaldi, A., Milanesi, C., Tognon, G., 1977. Hematopoeisis in Decapoda: Origin and evolution of hemocytes and cyanocytes of Carcinus maenas. Cell Differ. 6, 167-186.
Herández-López, J., Gollas-Galván, T.S., Vargas-Albores, F., 1996. Activation of the prophenoloxidase system of the brown shrimp (Penaeus californiensis Holmes). Comp. Biochem. Physiol. 113C, 61-66.
Hose, J.E., Martin, G.C., 1989. Defence functions of granulocytes in the ridgeback prawn Sicyonia ingentis. J. Invert. Pathol. 53, 335-346.
Jaing, Y., Zhong, G.G., Chen, L., Ma, X.Y., 1992. Influences of ginsenosides Rb1, Rb2 and Rb3 on electric and contractile activities of normal and damaged cultured myocardiocytes. Chung Kuo Yao Li Hsueh Pao 13, 403-406.
Johansson, M.W., Söderhäll, K., 1989. A cell adhesion factor from crayfish hemocytes has degranulating activity towards crayfish granular cells. Insect Biochem. 2, 183-190.
Kang, S.Y., Lee, K.Y., Lee, S.K., 1994. Ginsenoside-Rg1 regulates the induction of tyrosine amminotransferase gene transcription in rat hepatocyte cultures. Biochem. Biophys. Res. Commun. 205, 1696-1701.
Kim, N.D., Kang, S.Y., Kim, M.J., Park, J.H., Schini, K.V., 1999. The ginsenoside Rg3 evokes endothelium-independent relaxation on rat aortic ring: role of K+ channels. Eur. J. Pharmacol. 367, 51-57.
Le Moullac, G., Le Grounmellec, M., Ansquer, D., Froissard, S., Levy, P., Aquscop., 1997. Heamotological and phenoloxidase activity changes in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis. Fish Shellfish Immunol. 7, 227-234.
Lee, S.J., Sung, J.H., Lee, S.J., Moon, C.K., Lee, B.H., 1999. Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin. Cancer Lett. 144, 39-43.
Muñoz, M., Cedeño, R., Rodríguez, J., Knaap, W.P.W., Mialhe, E., Bachère, E., 2000. Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus vannamei. Aquaculture 191, 89-107.
Nickerson, K.W., Vanholde, K.E., 1971. A comparison of molluscan and arthropod hemocyanin- circular dichroism and absorption spectra. Comp. Biochem. Physiol. 39B, 855-872.
Onning, G., Wang, Q., Westrom, B.R., Asp, N.G., Karlsson, S.W., 1996. Influence of oat saponins on intestinal permeability in vitro and in vivo in the rat. Br. J. Nutr. 76, 141-151.
Perazzolo, L.M., Barracco, M.A., 1997. The prophenoloxidase activating system of the shrimp Penaeus paulensis and associated factors. Dev. Comp. Immunol. 21, 385-395.
Pére-Farfante, I., Kensley, B.F., 1997. Penaeoid and Sergestoid shrimps and prawns of the world: Keys and diagnoses for the families and genera. Mem. Mus. natl. Hist. Nat. 175, 1-233.
Preaux, G., Gielens, C., 1984. Hemocyanins in copper protein and copper enzymes. CRC Rress, Boca Raton, Florida, 159-205 pp.
Rodríguez, J., Le Moullac, G., 2000. State of the art of immunological tools and health control of penaeid shrimp. Aquaculture 191, 109-119.
Roy, P.K., Munshi, J.D., 1989. Effect of saponin extracts on oxygen uptake and haematology of an air-breathing climbing perch, Anabas testudineus (Bloch). J. Freahwater Biol. 1, 167-172.
Roy, P.K., Munshi, J.D., Dutta, H.M., 1990. Effect of saponin extracts on morpho-history and respiratory physiology of an air breathing fish, Heteropneustes fossilis (Bloch). J. Freshwater Boil. 2, 135-145.
Salim, K.N., McEwen, B.S., Chao, H.M., 1997. Ginsenoside Rb1 regulates ChAT, NGF and trkA mRNA expression in the rat brain. Brain Res. Mol. 47, 177-182.
Senkbeil, E.G., Wriston, J.C.J., 1981. Hemocyanin synthesis in the American lobster (Homarus americanus). Comp. Biochem. Physiol. 68B, 163-171.
Serrano Jr., A., Focken, U., Francis, G., Makkar, H.P.S., Becker, K., 1998. Effects of Quillaja saponins on the activity of selected gut and liver enzymes of carp, Cyprinus carpio. In: Jarayabhand, P., Chaitanawisuti, N., Sophon, A., Kritsanapuntu, A., Panichpol, A. (Eds.), The Fifth Asian Fisheries Forum, International conference on Fisheries and Food Security Bevond the Year 2000. Asian Fisheries Society, Bangkok, Thailand, pp. 204.
Shibata, S., 1993. Chemistry and cancer preventing cativities of ginseng saponin and some related triterpenoid compounds. korean Med. J. 16(suppl), 28-37.
Söderhäll, K., Cerenius, L., 1992. Crustacean immunity. Ann. Rev. Fish Dis. 3-23.
Söderhäll, K., Smith, V.J., Johansson, M.W., 1986. Exocytosis and uptake of bacteria by isolated hemocytes populations of two crustacean: Evidence for cellar cooperation in the defense reactions of arthropods. Cell Tissue Res. 245, 43-49.
Song, Y.L., Hsieh, Y.T., 1994. Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev. Comp. Immunol. 18, 201-209.
Song, Y.L., Lien, T.W., Yu, C.I., Lin, M.N., Huang, C.C., 2000. Hemolymph analysis of pacific white shrimp (Litopenaeus vannamei) infected with Taura syndrome virus., FA COA Aquaculture Series No.1, Reports of Fish Disearch, pp. 95-116.
Sun, H., Yang, Z., Ye, Y., 2006. Structure and biological activity of protopanaxatriol-type saponins from the roots of Panax notoginseng. Int. Immunopharmacol. 6, 14-25.
Sung, H.H., Yang, Y.L., Song, Y.L., 1996. Enhancement of microbicidal activity in the tiger shrimp Penaeus monodon via immunostimulation. J. Crustacean Biol. 16, 278-284.
Uglow, R.F., 1969. Haemolymph protein concentations in protunid crabs-2: The effects of imposed fasting on Carcinus maenas. Comp. Biolchem. Physiol. 31, 959-967.
Vargas-Albores, F., Guazmán, M.A., Ochoa, J.L., 1993. A lipopolysaccharide-binding agglutinin isolated from brown shrimp hemolymph. Comp. Biochem. Physiol. 104A, 407-413.
Wakabayashi, C., Murakami, K., Hasegawa, H., Murata, J., Saiki, I., 1998. An intestinal bacterial metabolite of ginseng protopanaxadiol saponins has the ability to induce apoptosis in tumor cells. Biochem. Biophys. Res. Commun. 246, 725-730.
Yokozawa, T., Yasui, T., Oura, H., 1996. Molecular biological analysis of the effects of ginsengoside-Rb2 on albumin mRNA in streptozotocin-induced diabetic rats. J. Pharm. Pharmacol. 48, 763-767.
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