臺灣博碩士論文加值系統

近年來在養殖魚類中發現疑似虹彩病毒的報告愈來愈多，但是這些分離株在虹彩病毒科內的分類地位並不明確，因此本論文延續之前研究，將研究室自1995到2004年間，分離自本省養殖石斑、金目鱸、龍膽與海鱺的虹彩病毒分離株，以及近期收集到疑似虹彩病毒感染之檢體鱼，進行細胞病理表現和特定基因序列分析比對。首先將檢體魚以石斑魚泳鰾細胞株 (SB cells) 進行病毒分離為初步檢測，結果有兩個檢體 (B和K) 出現和石斑虹彩病毒一樣細胞變大變圓的的細胞病變效應(Cytopathic effect, CPE)；而檢體F、I、J、U和M接種後，部份細胞也呈現變圓的CPE，但較不明顯。另外，檢體C、G、Q和S接種後，細胞呈現拉長而非變大變圓，這和來自金目爐的95u323造成的CPE相似。除此之外，檢體A、T和V接種結果和前兩者都不同，細胞則是出現顆粒化現象。進一步將所有檢體濾液以人工腹腔注射感染魚體，之後進行肥大細胞之細胞病理表現觀察。結果檢體B、F、G、J、M和S的感染魚臟器內皆可觀察到嗜鹼性肥大細胞，這和已知的虹彩病毒株，日本嘉鱲虹彩病毒(RSIV)、台灣石斑虹彩病毒(TGIV-1、TGIV-2)、龍膽石斑類虹彩病毒(GGILV) 的病理表現一致。另外，金目鱸類虹彩病毒 (PILV)感染魚的肥大細胞表現不明顯，而海鱺類虹彩病毒(CILV) 和石斑虹彩病毒(GIV)感染魚臟器內則完全觀察不到肥大細胞。由此結果初步判定，這些分離株和檢體在虹彩病毒科中的分類，可能分屬Megalocytivirus和Ranavirus兩屬。所以最後針對Megalocytivirus屬與Ranavirus屬的特定基因序列引子進行PCR的分析比較。選擇的引子包括針對Megalocytivirus屬MCP (major capsid protein)設計的MCIV MCP C50/C51、CY15n及其nested RY16，RSIV ATPase 3-F/3-R和RSIV PstⅠ fragment的 1-F/1-R；另外Ranavirus屬則選擇GIV PNP基因的 F/R1引子。結果略為分出RSIV、TGIV-1、GGILV、PILV基因型較為相似，歸屬Megalocytivirus，而TGIV-2、CILV、GIV基因型相似，較接近Ranavirus。藉由肥大細胞病理表現和特定基因序列分析，推測本省養殖魚類的虹彩病毒來源可能不同，有Megalocytivirus屬的TGIV-1、GGILV、PILV和新分離出之虎斑B (940707B)、F (940916B)、北門地區龍膽G (940922G)、七股龍膽石斑J (941026G)、點帶石斑M (941129O)、七星鱸Q (950124J)、金目鱸S (950125G) 及海鱺V (950508C)；而TGIV-2和CILV則可能歸入Ranavirus屬。

In the recent year, many iridoviral-like infections has been reported. But the classification of these iridovirus were not clear enough, so this thesis continued the previous research for classification of fish iridovirus. From 1995 to 2004, we collected the iridovirus-like isolates from agriculture grouper, perch, giant grouper, cobia and some other iridovirus-like isolates that collected after that collected afterward. We compared the cytopathological presentation and sequence analysis of particular viral genes of iridovirus isolates.First, preliminary examine was specimens had been used cell line from swimbladder of grouper to virus isolate. The result of two specimens B and K appeared CPE the same as grouper iridovirus infected cells that became large and round. However, after specimens F,I,J,U and M infected the cell line, cells in part appeared CPE that cells became round but not apparent. In addition, after specimens C,G, Q and S infected cells, cell presented stretch neither large nor round, this situation was similar the CPE from 95u323 isolates of perch. In addition of specimens A,T and V were results different from the preceding, cells appered the phenomenon of granulation.Further, the filtrates of all specimens were infected intraperitoneally in fish, then we observed cytopathological presentation with hypertrophy cell. The result was the viscera which infected fish by specimens B,F,G,J,M and S all could been observed basophilic enlarged cell. That cytopathological presentation was the same with known iridovirus isolates, red seabeam iridovirus (RSIV), grouper iridovirus of Taiwan-1(TGIV-1), grouper iridovirus of Taiwan-2(TGIV-2)and giant grouper iridovirus-like(GGILV). In additional, cytopathological presentation with hypertrophy cell that PILV infected fish not was apparent. However, cobia iridovirus-like(CILV)and grouper iridovirus(GIV)infected fish not to observe completely the enlarged cell in the viscera. Therefore, these results preliminary determined the classification of these known isolates and specimens in Iridovirus, to probably be belong to two genus Megalocytivirus and Ranavirus. Finally, amplified PCR products for the primers of particular viral gene sequence in the genus of Megalocytivirus and Ranavirus were analyzed and compared. Choice primers included MCIV MCP C50/C51,CY15n and nested RY16 primers to design for MCP(major capsid protein) of Megalocytivirus, RSIV ATPase 3-F/3-R and RSIV PstⅠ fragment 1-F/1-R to design for Megalocytivirus; in another hand, we chosen GIV PNP F/R1 primer for Ranavirus. The results slightly sorted genotype RSIV, TGIV-1, GGILV and PILV to belong to Megalocytivirus, then genotype TGIV-2, CILV and GIV to close to Ranavirus.By hypertrophy cytopathological presentation and sequence analysis of particular viral genes suggested the origin of iridovirus isolates from maricultured fishes in Taiwan maybe differ, like as TGIV-1, GGILV, PILV, and new isolates: B (940707B) and F (940916B) from brown marbled grouper, G (940922G) from giant grouper in BeiMen area, J (941026G) from giant grouper in ChiGu area, Orange-spotted grouper M (941129O), Japanese seaperch Q (950124J) , seaperch S (950125G) and cobia V (950508C) of the genus of Megalocytivirus; however,as TGIV-2 and CILV were belong to the genus of Ranavirus

中文摘要i
英文摘要iii
目錄v
圖目錄vii
表目錄ix
前言1
文獻整理3
一、虹彩病毒科的簡介以及分類依據3
二、魚類虹彩病毒之介紹5
三、亞洲地區海水魚類虹彩病毒感染症相關疫情報導7
四、亞洲地區魚類虹彩病毒之基因分析比較9
五、限制性多重蛋白質序列與結構排比14
材料與方法15
一、疑似虹彩病毒感染海水魚檢體之收集15
（一）、實驗材料15
1、檢體來源15
2、細胞株15
3、虹彩病毒15
（二）、研究方法15
1、檢體之背景資料訪查15
2、檢體之病毒分離試驗16
二、魚類虹彩病毒與各分離株之細胞病理表現觀察18
（一）、實驗材料18
1、已知之魚類虹彩病毒18
2、病毒分離陽性之檢體19
（二）、研究方法19
1、魚類虹彩病毒人工感染魚之組織細胞病理觀察20
2、檢體濾液感染魚之組織細胞病理觀察20
三、魚類虹彩分離株之特定基因序列分析與比較21
（一）、實驗材料21
1、Megalocytivirus特定基因序列之引導子21
2、Ranavirus特定基因序列之引導子22
（二）、研究方法22
1、魚類虹彩分離株之特定基因序列分析與分群22
(1) DNA 之萃取22
(2)魚類虹彩病毒與分離株之 PCR 反應23
(3)片段 PCR 序列比對分析24
結果25
討論29
參考文獻35
圖表43
附錄66

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