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研究生:林韋利
研究生(外文):Wei-Li Lin
論文名稱:乙烯及1-Methylcyclopropene對蝴蝶蘭花朵壽命之影響
論文名稱(外文):Effects of Ethylene and 1-Methylcyclopropene on Flower Longevity in Phalaenopsis amabilis
指導教授:張耀乾張耀乾引用關係
指導教授(外文):Yao-Chien Chang
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:園藝學研究所
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:82
中文關鍵詞:蝴蝶蘭乙烯1-methylcyclopropene花朵壽命採後生理
外文關鍵詞:Phalaenopsisethylene1-methylcyclopropeneflower longevitypostharvest
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本研究以台灣蝴蝶蘭(Phalaenopsis amabilis)為植物材料,研究乙烯及1-methylcyclopropene (1-MCP)對花朵壽命的影響,探討蝴蝶蘭花序上不同發育階段之花朵對於乙烯敏感度的差異、乙烯作用抑制劑1-MCP對於蝴蝶蘭的絕對保護效期(本研究定義絕對保護為完全不受2 μL.L-1乙烯之影響)及欲達到絕對保護成效所需的處理條件,以提供Phal. amabilis前處理1-MCP的精確資料。
台灣蝴蝶蘭遭遇2 μL.L-1乙烯逆境會造成花朵及花苞快速萎凋,以較低濃度(0.3 μL.L-1)處理,觀察到花序上不論是已綻放的花朵或發育中的花苞,皆隨發育階段的成熟對於乙烯的敏感度提高,且花朵又較花苞敏感。同一花序中,成熟的花朵遭遇0.3 μL.L-1乙烯逆境後,於第26小時有乙烯大量產生的高峰;但最幼嫩的花朵,在處理乙烯後,乙烯產生量與對照組無異。
台灣蝴蝶蘭前處理1-MCP可以抑制外施乙烯造成的花朵萎凋,前處理濃度或時間會影響1-MCP對蝴蝶蘭的保護成效,而1-MCP的最低有效濃度似隨植株生理狀況及處理之季節變化而異。本研究結果顯示蝴蝶蘭盆花前處理1-MCP 12小時,縱使濃度低至0.005 μL.L-1,仍可降低花朵萎凋率,亦即只要有1-MCP的保護皆可減緩乙烯所造成的花朵快速萎凋,然欲達絕對保護成效,所需最低濃度為0.1 μL.L-1;前處理時間並可大幅縮短,1-MCP前處理濃度為0.05 μL.L-1時,處理時間應長於1小時,而處理濃度提高為0.2 μL.L-1時,只要處理0.5小時亦可達到絕對保護成效。此外即使將蝴蝶蘭花朵遭遇的乙烯逆境提高至20 μL.L-1,前處理蝴蝶蘭0.1 μL.L-1 1-MCP 12小時的保護成效不受影響。
蝴蝶蘭以0.8 μL.L-1 1-MCP前處理8小時,再分別於處理後不同天數給予乙烯逆境,比較花朵萎凋情形,結果顯示1-MCP對Phal. amabilis花朵的絕對保護效期約為6-8天;為瞭解多次施用1-MCP對於保護效期的影響,分別對蝴蝶蘭前處理一、二或三次之1-MCP,而後於不同天數給予2 μL.L-1乙烯逆境,由試驗結果得知,多次施用1-MCP可以延長保護效期,且三次施用可使1-MCP對台灣蝴蝶蘭的保護效期最少延長到24天。
In order to obtain information on anti-ethylene protection of 1-Methyl -cyclopropene (1-MCP; an ethylene action inhibitor ) in Phalaenopsis amabilsi, sensitivity to ethylene of flowers and buds in different developing stages, the residual effect of 1-MCP, and the interaction of 1-MCP concentration and fumigation time were determined.
Flower wilting of Phal. amabilis was induced by 2 μL.L-1 ethylene. Sensitivity to ethylene increased as the maturity of flowers or buds increased. However, flowers were more sensitive to ethylene than buds. Ethylene production was increased when full-opened flower were exposed to 0.3 μL.L-1 ethylene, and peaked 26 hours after treatment. However, ethylene production was similar in ethylene-treated and untreated young buds.
Pretreating Phalaenopsis amabilis with 1-MCP inhibited ethylene-induced senescence. However, effectiveness of 1-MCP depends on treatment duration and concentration. The data show pretreated Phal. amabilis with 1-MCP for 12 hours decresed flower wilting even at a very low concentration of 0.005 μL.L-1. Nevertheless, for a complete blocking of ethylene action (no damages by 2 μL.L-1 ethylene), a concentration of 0.1 μL.L-1 was required. The fumigation duration can be reduced to 1 hour. Moreover, the treatment duration should be longer than 1 hour when 1-MCP concentration was 0.05 μL.L-1; but a higher 1-MCP concentration at 0.2 μL.L-1 for a short duration (a half hour) was effective. In addition, pretreating Phal. amabilis with 0.1 μL.L-1 1-MCP for 12 hours was effective to inhibit 20 μL.L-1 ethylene from action.
By pretreating Phal. amabilis with 0.8 μL.L-1 1-MCP for 8 hours on Day 0, followed by 2μL.L-1 ethylene fumigation for 12 hours on designate days, we found the residual protection of 1-MCP on Phal. amabilis was approximately 6-8 days. Furthermore, multiple applications of 1-MCP extended the duration of 1-MCP protection against ethylene. Three times applications increased the residual protection of 1-MCP on Phal. amabilis to at least 24 days.
摘要 1
第一章 緒論 3
第二章 1-METHYLCYCLOPROPENE對蝴蝶蘭花朵的保護效期 7
前言 INTRODUCTION 7
材料與方法 MATERIALS AND METHODS 8
植物材料 8
1-Methycyclopropenl及乙烯的施用 8
乙烯濃度分析 9
試驗一: 1-Methylcyclopropene對蝴蝶蘭的保護效期I 9
試驗二: 1-Methylcyclopropene對蝴蝶蘭的保護效期II 9
試驗三: 多次施用1-methylcyclopropene對蝴蝶蘭保護效期之影響 I 10
試驗四: 多次施用1-methylcyclopropene對蝴蝶蘭保護效期之影響 II 10
資料分析 11
結果 RESULT 11
試驗一: 1-Methylcyclopropene對蝴蝶蘭的保護效期I 11
試驗二: 1-Methylcyclopropene對蝴蝶蘭的保護效期II 12
試驗三: 多次施用1-methylcyclopropene對蝴蝶蘭保護效期之影響 I 13
試驗四: 多次施用1-methylcyclopropene對蝴蝶蘭保護效期之影響 II 13
討論 DISCUSSION 14
第三章 前處理1-METHYLCYCLOPROPENE之濃度及時間對蝴蝶蘭保護效果的影響 24
前言 INTRODUCTION 24
材料與方法 MATERIALS AND METHODS 25
試驗一: 長時間前處理下1-methylcyclopropene的最低有效濃度 25
試驗二: 短時間前處理下1-methylcyclopropene的最低有效濃度I 25
試驗三: 短時間前處理下1-methylcyclopropene 最低有效濃度II 26
試驗四: 1-Methylcyclopropene 濃度與處理時間對保護效果的影響 26
試驗五: 乙烯濃度對1-methylcyclopropene保護效果的影響 26
結果 RESULTS 27
試驗一: 長時間前處理下1-methylcyclopropene的最低有效濃度 27
試驗二: 短時間前處理下1-methylcyclopropene的最低有效濃度 I 28
試驗三: 短時間前處理下1-methylcyclopropene 最低有效濃度II 28
試驗四: 1-Methylcyclopropene濃度與處理時間對保護效果的影響 29
試驗五: 乙烯濃度對1-methylcyclopropene保護效果的影響 30
討論 DISCUSSION 30
第四章 蝴蝶蘭不同發育階段花朵對乙烯敏感度的差異 48
前言 INTRODUCTION 48
材料與方法 MATERIALS AND METHODS 49
二氧化碳濃度分析 Analysis of carbondioxide gas 49
試驗一: 不同發育階段花朵及花苞對乙烯敏感度的差異 I 50
試驗二: 不同發育階段花朵及花苞對乙烯敏感度的差異 II 50
試驗三: 蝴蝶蘭花序上第一朵花之乙烯及二氧化碳釋放量的變化 50
試驗四: 不同發育階段花朵及花苞的乙烯及二氧化碳釋放量 51
試驗五: 不同發育階段花朵及花苞1-aminocyclopropane-1-carboxylate (ACC) oxidase活性的差異 51
結果 RESULTS 52
試驗一: 不同發育階段花朵及花苞對乙烯敏感度的差異I 52
試驗二: 不同發育階段花朵及花苞對乙烯敏感度的差異II 53
試驗三: 蝴蝶蘭花序上第一朵花之乙烯及二氧化碳釋放量的變化 54
試驗四: 不同發育階段花朵及花苞的二氧化碳釋及乙烯放量 54
試驗五: 不同發育階段花朵及花苞1-aminocyclopropane-1-carboxylate (ACC) oxidase活性的差異 56
討論 DISCUSSION 56
SUMMARY 73
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附錄 80
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