臺灣博碩士論文加值系統

本研究探討靜電紡絲製程中電場電壓、溶液濃度、距離等條件，以明膠（gelatin）為溶質，甲酸、乙醇水溶液為溶劑所構成的溶液製備具備奈米結構之不織布薄膜，以得到各種不同纖維直徑的薄膜。由實驗結果，可得知最佳條件為濃度15%，噴絲距離15cm，電壓20kV，可得纖維之平均尺寸為50~100nm。所得之明膠不織布，用戊二醛（glutaraldehyde）交聯，同時量測活化部分凝血酶時間(APTT)、血小板凝集程度來評估凝血性質。此外明膠不織布在細胞植入效率和增生性測試，以CRL 1927腎臟間質細胞體外培養測試，結果發現明膠不織布會有很高的細胞植入效率且能促進腎臟間質細胞的增生。

This research discusses the effect of the electrospinning conditions including voltage, concentration, and distance on the thermal and biological properties of gelatin fibers. The spinning dope consisted of gelatin, formic acid, ethanol, and water as solvent. The results show that the gelatin fiber spun with 20kV from the 15% dope solution exhibited better nanostructure. The highest uniformity in the fiber diameter was attained when the distance was 15cm. After crosslinking with glutaraldehyde, the nonwoven exhibited higher thermal stability. In addition, gelatin non-woven exhibits a higher cell seeding efficiency than the control (TCPS) and also promotes CRL 1927 proliferation.

摘要 i
ABSTRACT ii
誌謝 iii
第一章 緒論 1
1.1研究背景 1
1.2研究目的 1
1.2.1奈米材料 2
1.2.2奈米纖維 3
第二章 文獻回顧 4
2.1靜電紡絲工程與原理 4
2.2組織工程 5
2.3明膠簡介 5
2.4血液凝固學理論概要 6
2.4.1凝血作用程序 7
2.4.2血液的凝固機制 9
2.4.3活化部分凝血活酶時間(activated partial thromboplastin time, APTT ) 9
2.5血小板之簡介 10
2.6高分子生醫材料 10
第三章 實驗材料與方法 12
3.1實驗藥品 12
3.2實驗設備 13
3.3實驗流程 14
3.4明膠凝結點測定 14
3.5黏度測定 15
3.6掃瞄式電子顯微鏡（SEM） 16
3.7薄膜製備 16
3.8明膠熱性質分析 17
3.9凝血實驗 18
3.10血小板附著評估 18
3.11細胞相容性 19
3.11.1明膠不織布之細胞培養技術 19
3.11.2細胞濃度之計數方式 19
3.11.3細胞增生實驗 20
3.11.4細胞增生能力測定(MTT assay) 20
第4章 結果與討論 21
4.1凝結點測定 21
4.2黏度測定 22
4.3 電子顯微鏡 25
4.3.1 尺寸分佈 31
4.4 製程變因與薄膜結構關係 34
4.4.1 溶液濃度與纖維直徑關係 34
4.4.2 射出電壓與纖維直徑關係 35
4.4.3噴絲距離對尺寸分佈之影響 36
4.5 DSC測試 36
4.6 TGA測試 38
4.7凝血時間實驗 39
4.8血小板吸附實驗 40
4.9 廣角X-ray 繞射分析 41
4.10 腎臟間質細胞培養 43
第五章 結論 45
第六章 參考文獻 46
作者簡介 50

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