臺灣博碩士論文加值系統

白血球在血管管壁的黏附與移行是動脈粥狀硬化進展的早期主要因素，且血管內皮細胞表現的黏附因子在動脈粥狀硬化過程中扮演重要角色。抗氧化物具有抑制細胞黏附分子表現，進而可降低動脈粥狀硬化的發生作用。故本研究主要探討具有抗氧化的紫色蕃薯葉萃曲液及其主要成份cyanidin與quercetin，對於前發炎因子腫瘤壞死因子α刺激人類主動脈內皮細胞之黏附因子與NF-κB表現的影響。將人類主動脈內皮細胞預先以紫色蕃薯葉萃取物（100 μg/mL）、quercetin（10μM）和cyanidin（10μM）處理18小時，可顯著性減少人類單核球細胞株U937的黏附。而使用細胞酵素連結免疫吸光分析法，顯示紫色蕃薯葉萃取物及其主要成份quercetin和cyanidin可顯著降低血管黏附分子表現分別達（VCAM-1）54％、28％、37％，但只有紫色蕃薯葉萃取物及Quercetin顯著降低細胞間黏附分子（ICAM-1）表現分別達19％、38％表現；對內皮細胞黏附因子（E-selectin）的表現則不具影響。並且紫色蕃薯葉萃取物降低NF-κB p65 表現，可能造成抑制NF-κB表現，進而影響黏附因此可能在延緩動脈粥狀硬化與發炎反應因子表現。

The adhesion and migration of circulating leukocytes into the vessel wall is an early event in atherogenesis and the expression of cell adhesion molecules by the arterial endothelium plays a major role during atherogenesis. Antioxidants have been proposed to inhibit the expression of adhesion molecules and there by attenuate the processes leading to atherosclerosis. In this study, the effects of antioxidant extract from purple sweet potato leaves and its major components cyanidin and quercetin on the expression of adhesion molecules and NF-κB in tumor necrosis factor-a (TNF-a) treated human aortic endothelial cells (HAEC) were investigated. Pretreated of HAEC with purple sweet potato leaves (100 μg/mL), quercetin (10 μM), cyaniding (10 μM) for 18h significantly suppressed cellular binding between the human monocytic leukemic cell line-U937 and TNF-α-stimulated HAEC by using an in vitro binding assay.
Cell-enzyme-linked immunosorbent assay (Cell-ELISA) and immunoblot analysis showed that pretreated of HAEC with purple sweet potato leaves extracts (100 μg/mL) , quercetin (10 μM) and cyanidin (10 μM) for 18h significantly attenuated vascular cell adhesion molecule-1 (VCAM-1)expression by 54％,28％and 37％respectively. But only intercellular cell adhesion molecule-1(ICAM-1) expression by 19％and 38％recpectively. But only purple sweet potato leaves extracts and quercetin signifieantly attenuated intercellular cell. However, there had no effect on expression of endothelial cell E-selectin. Meanwhile, purple sweet potato leaves extracts significantly decreased the expression of NF-κB p65 witch may decrease the activity of NF-κB and further influence on the expression of adhesion molecules and there by attenuate the atherosclerosis and inflammatory responses.

目 錄
頁次
第一章 緒論 1
第一節 研究動機 2
第二節 研究目的 5
第二章 文獻探討 6
第一節 動脈血管粥硬化的成因 7
第二節 腫瘤壞死因子(Tumor necrosis factor-alpha)於發炎反應之功能與作用機制 11
第三節 動脈血管粥狀硬化與可反應性氧(Reactive Oxygen Species)相關性 15
第四節 動脈血管粥狀硬化與細胞黏附分子之相關性 18
第五節 細胞激素之功能 21
第六節 NF-κB調控發炎基因表現之機轉 24
第七節 植物化學物質 28
第三章 材料與方法 34
第一節 實驗架構 35

第二節 HPLC分析 36
第三節 細胞培養與各項分析內容 40
第四章 實驗結果 57
第五章 討論與結論 62
附圖 71
參考文獻 88

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