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研究生:劉方欣
研究生(外文):Fang-Hsin Liu
論文名稱:視網醇乙酸對食鹽水誘發大鼠肺損傷發炎反應之影響
論文名稱(外文):Effect of retinol acetate on inflammation by saline induced lung injury of rat
指導教授:黃延君
指導教授(外文):Yan-Jiun Huang
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:59
中文關鍵詞:白血球著邊作用玻璃質膜發炎反應視網醇乙酸食鹽水誘發肺損傷
外文關鍵詞:hyaline membraneleukocytes marginationinflammationretinol acetatesaline induced lung injury
相關次數:
  • 被引用被引用:1
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摘要
呼吸系統疾病一直是全球人類所困擾的問題。維生素A與肺
組織的修復及發炎反應關係密切。本研究目的為探討維生素A(視
網醇乙酸)對食鹽水誘發大鼠肺損傷之影響。
實驗將Sprague–Dawley大鼠的支氣管肺泡施予4 ml生理食鹽
水進行4次灌洗誘發急性肺損傷。接著將大鼠分別給予0.01、
0.05、0.1 μg的視網醇乙酸處理60分鐘。實驗處理後,將大鼠安
樂死並測定支氣管肺泡灌洗液中的白血球數量。另外將損傷的肺
組織取下並於顯微鏡下鑑定損傷。結果發現支氣管肺泡灌洗會誘
發肺組織玻璃質膜的形成、多型核白血球的著邊作用及出血情
形,然而在視網醇乙酸處理下均可顯著降低玻璃質膜的形成。另
外0.01 μg視網醇乙酸可顯著降低多型核白血球的著邊作用。0.05
μg與0.1 μg視網醇乙酸處理可顯著降低肺間質與肺泡內水腫情
形。然而這些劑量的視網醇乙酸處理對白血球數量無顯著的不同。
綜合以上結果建議,適量視網醇乙酸處理對於支氣管肺泡灌
洗造成的大鼠肺臟損傷及所引發炎症反應之玻璃質膜現象、多型
核白血球的著邊作用與肺間質與肺泡內水腫情形有所改善,但其
機制仍需更進ㄧ步探討。
Abstract
Respiratory diseases are always a worldwide human question perplexed. Vitamin A has closely relationshipinvolved in the repairing and inflammatory reaction of lung tissue. The purpose of this study
was to investivgate the effect of vitamin A (retinol acetate) on saline induced lung injury of rat.

The bronchial alveoli of Sprague–Dawley rat were washed with 4 ml saline for four times to induce rat acute lung injury. Then the rats were treated with 0.01, 0.05 or 0.1 μg of retinol acetate for 60 minutes, respectively. After the treatment, rats were euthanized and white cell number in bronchial alveolar fluid was determined. In addition, the injury pulmonary tissue was isolated and identified the damage under microscope. The result showed that bronchialalveolar lavage induced hyaline membrane formation, polymorphonuclear leukocytes margination and hemorrhage in lung tissue, whereas, the administration of retinol acetate reduced hyaline membrane formation significantly. In addition, 0.01 μg retinol acetate reduced polymorphonuclear leukocytes margination. Both the administration of retinol acetate at the dosage level of 0.05 and 0.1 μg reduced interstitial and intraalveolar edema significantly. However, there was not any significant difference in the number of leukocytes between the administrated dosage levels of retinol acetate.
The results suggested that moderate dose of retinol acetate treatment significantly improved the lung injury and the inflammatory phenomenon of hyaline membrane formation, polymorphonuclear leukocytes margination, interstitial and intraalveolar edema which caused by bronchoalveolar lavage. Further experiments is necessary to elucidate the mechanism.
中文摘要……………………………………………………… Ⅰ
英文摘要……………………………………………………… Ⅲ
誌謝…………………………………………………………… Ⅴ
目錄…………………………………………………………… Ⅵ
表目錄………………………………………………………… Ⅹ
圖目錄………………………………………………………… ⅩI
第一章 緒言………………………………………………… 1
第二章 文獻回顧…………………………………………… 3
ㄧ、肺損傷………………………………………………… 3
二、維生素A……………………………………………… 9
(一)維生素A形式與來源……………………………… 9
(二)生理功能…………………………………………… 11
(三)維生素A與呼吸系統……………………………… 12
(四)維生素A與發炎…………………………………… 13
第三章 材料與方法…………………………………………. 16
一、實驗對象……………………………………………… 16
二、實驗材料……………………………………………… 16
三、實驗方法……………………………………………… 18
(一)實驗分組…………………………………………… 18
(二)大鼠手術前處理…………………………………… 19
(三)實驗步驟…………………………………………… 20
(四)白血球細胞總數計數……………………………… 21
(五)白血球細胞分類…………………………………… 21
(六)肺組織損傷程度的判定與分級…………………… 22
四、統計分析……………………………………………… 22
第四章 結果…………………………………………………. 23
ㄧ、支氣管肺泡灌洗液中白血球數量變化………………. 23
(一)不同處理組對大鼠支氣管肺泡灌洗液中總白血
球細胞數量之影響……………………………………. 23
(二)不同處理組對大鼠支氣管肺泡灌洗液中單核球
細胞數量之影響………………………………………. 23
(三)不同處理組對大鼠支氣管肺泡灌洗液中淋巴球
細胞數量之影響………………………………………. 24


(四)不同處理組對大鼠支氣管肺泡灌洗液中嗜中性
白血球細胞數量之影響………………………………. 24
二、 大鼠肺組織之變化……………………………………. 24
(一)玻璃質膜之形成……………………………………... 24
1、支氣管肺泡灌洗術對肺組織玻璃質膜形成之比較… 24
2、視網醇乙酸處理對肺組織玻璃質膜形成之比較…… 25
(二)多型核白血球著邊作用之形成……………………… 26
1、支氣管肺泡灌洗術對肺組織多型核白血球著邊
作用之比較……………………………………………. 26
2、視網醇乙酸處理對肺組織多型核白血球著邊作用
之比較…………………………………………………. 26
(三)肺間質與肺泡內水腫之變化………………………… 26
1、支氣管肺泡灌洗術對肺間質與肺泡內水腫之比較…. 27
2、視網醇乙酸處理對肺間質與肺泡內水腫之比較……. 28
(四)血管周圍與肺泡內出血之變化……………………… 28
1、支氣管肺泡灌洗術對血管周圍與肺泡內出血之比較. 28
2、視網醇乙酸處理對血管周圍與肺泡內出血之比較…. 29
第五章 討論………………………………………………… 31
第六章 結論………………………………………………… 43
第七章 參考文獻…………………………………………… 45
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