(44.192.112.123) 您好!臺灣時間:2021/03/04 06:05
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:莊盛豪
研究生(外文):Sheng-Hao Chuang
論文名稱:大鼠自發性棘徐波與性別、年齡、似焦慮及似憂鬱行為關係之研究
論文名稱(外文):Relation of spontaneous spike wave discharge with gender, age,anxiety-like and depression-like behaviors in rats
指導教授:謝坤叡
指導教授(外文):Kun-Ruey Shieh
學位類別:碩士
校院名稱:慈濟大學
系所名稱:神經科學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:50
中文關鍵詞:Long Evans 大鼠年齡性別似憂鬱似焦慮失神性癲癇
外文關鍵詞:Long Evans ratsagegenderdepression-likeabsence seizureanxiety-like
相關次數:
  • 被引用被引用:2
  • 點閱點閱:254
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
在Long Evans (LE)大鼠長時間腦電波記錄中常觀察到一種自發性7-12 Hz棘徐波放電(spike-wave discharge, SWD),經由電生理及藥理測試後已證實此棘徐波放電與人類失神性癲癇類似。目前用來作為失神性癲癇動物模式多為近親交配(WAG/Rij)或是經由篩選(GAERS)而培育出的癲癇大鼠,不同於LE品系為自然非近親交配鼠種。爲建立LE大鼠完整的基礎資料,本實驗在3, 6, 12月大的公母鼠進行額葉腦電波記錄,並計算出在兩小時記錄中SWD產生次數,發現在三個月大LE大鼠已出現SWD,且次數會隨著年紀增長而增加,到六個月大時達到最高峰,但這個趨勢無性別差異。利用陰道抹片判斷三個月大大鼠的時動情週期,也發現在動情前期SWD次數與時間相較其於動情周期其他期間較高,而且在連續記錄腦電波48小時發現SWD具有日變節律。利用焦慮行為評估工具〈架高型十字迷宮、開放空間、亮暗箱〉以及憂慮行為評估工具〈強迫游泳測試〉來比較Wistar與LE大鼠行為參數,另外在LE大鼠給予Ethosuximide (ETX, 100 mg/kg, i.p.)以完全抑制SWD,以便探討SWD對於LE大鼠的行為試驗的影響。從架高型十字迷宮與開放空間可發現大鼠移動距離隨著SWD次數的增加而減弱,這樣的運動功能抑制情況在給予ETX後則恢復正常。相對於Wistar大鼠而言LE大鼠的似焦慮程度較低且似憂鬱程度較高。而且SWD次數與似焦慮程度呈現正相關,在LE大鼠給予ETX後這種相關顯著性則幾乎完全消失,但是在似憂鬱實驗沒有發現此特徵。基於以上結果,本研究了解LE大鼠的SWD產生與年齡有關而且到六個月後達到最大值,且SWD可能會對憂鬱及焦慮有關神經網絡有不同的影響。
Spontaneous paroxysmal 7-12 Hz spike-wave discharges (SWDs), which may be associated with absence seizures based on several lines of electrophysiological and pharmacological evidences, are often observed in Long Evans rats. Compared to well-developed absence epileptic rat models, i.e., WAG/Rij and GAERS strains, the development and gender sensitivity of SWDs in Long Evans rats remains largely unknown. Moreover, the correlate of SWDs and psychological performance also remains poorly understood. To investigate these issues, skull electrodes were implanted in six groups of Long Evans rats classified by gender and age (3, 6, 12 months). The number and durations of SWDs, which were identified from 2-h recording sessions, increased as age increased and reached the maximum at 6 months old. These SWD indexes revealed no difference in gender. Moreover, the occurrence of SWDs displayed a circadian rhythm from a continuous 48-h recording. The number and duration of SWDs were slightly higher at the proestreus stage of estrus cycle. Moreover, four behavioral paradigms, including anxiety-like behavioral test (elevated plus-maze, open field and light-dark box choice) and depression-like behavioral test (forced swimming) were used to evaluate the anxiety and depression levels in both Long Evans and Wistar rats. Changes of 4 behavioral tests by the administration of ethosuximide (ETX, 100 mg/kg i.p.) were also compared in Long Evans rats. Movement in the elevated plus maze and open field tests decreased as SWD number increased, and ETX restored the motor ability. Compared to Wistar rats, Long Evans rats were the propensity of low anxiety and high depression. In addition, increase of SWD numbers was prone to increase of anxiety-like behavior, and the trend was abolished by ETX. However, no obvious trend was found between depressive-like behaviors and SWD numbers before and after ETX injection. Based on these results, we conclude that the development of SWDs in Long Evans rats is age-dependent, and SWDs may affect both anxiety- and depression-associated neural circuits differentially.
中文摘要 1
英文摘要 3
前言 4
材料與方法 11
結果 16
討論 21
結論 29
參考文獻 30
附圖 34
Aldinio C, Aporti F, Calderini G, Mazzari S, Zanotti A, Toffano G (1985) Experimental models of aging and quinolinic acid. Methods Find Exp Clin Pharmacol 7:563-568.
Bauer MS (1990) Intensity and precision of circadian wheel running in three outbred rat strains. Physiol Behav 47:397-401.
Brito GN (1983) The behavior of vasopressin-deficient rats (Brattleboro strain). Physiol Behav 30:29-34.
Brito GN, Thomas GJ (1981) T-maze alternation, response patterning, and septo-hippocampal circuitry in rats. Behav Brain Res 3:319-340.
Burr W, Korner E, Stefan H (1991) Circadian distribution of generalized spike-wave activity in relation to sleep. Epilepsy Res Suppl 2:121-135.
Buzsaki G, Smith A, Berger S, Fisher LJ, Gage FH (1990) Petit mal epilepsy and parkinsonian tremor: hypothesis of a common pacemaker. Neuroscience 36:1-14.
Coenen AM, Van Luijtelaar EL (1987) The WAG/Rij rat model for absence epilepsy: age and sex factors. Epilepsy Res 1:297-301.
Coenen AM, Drinkenburg WH, Peeters BW, Vossen JM, van Luijtelaar EL (1991) Absence epilepsy and the level of vigilance in rats of the WAG/Rij strain. Neurosci Biobehav Rev 15:259-263.
Danober L, Deransart C, Depaulis A, Vergnes M, Marescaux C (1998) Pathophysiological mechanisms of genetic absence epilepsy in the rat. Prog Neurobiol 55:27-57.
Dorsa DM, Smith ER (1980) Facilitation of mounting behavior in male rats by intracranial injections of luteinizing hormone-releasing hormone. Regul Pept 1:147-155.
Fanselow EE, Sameshima K, Baccala LA, Nicolelis MA (2001) Thalamic bursting in rats during different awake behavioral states. Proc Natl Acad Sci U S A 98:15330-15335.
Fontanini A, Katz DB (2005) 7 to 12 Hz activity in rat gustatory cortex reflects disengagement from a fluid self-administration task. J Neurophysiol 93:2832-2840.
Franck MC, Kuhlo W (1970) [Effects of alcohol on directed saccadic eye movements in man]. Arch Psychiatr Nervenkr 213:238-245.
Jacoby A, Baker GA, Steen N, Potts P, Chadwick DW (1996) The clinical course of epilepsy and its psychosocial correlates: findings from a U.K. Community study. Epilepsia 37:148-161.
Kaplan BJ (1985) The epileptic nature of rodent electrocortical polyspiking is still unproven. Exp Neurol 88:425-436.
Karbowski A, Camps L (1985) [Morphological, morphometric and stereological aspects of Blount's unilateral stapling of the growth plate in animal experiments]. Z Orthop Ihre Grenzgeb 123:403-408.
Klein JP, Khera DS, Nersesyan H, Kimchi EY, Waxman SG, Blumenfeld H (2004) Dysregulation of sodium channel expression in cortical neurons in a rodent model of absence epilepsy. Brain Res 1000:102-109.
Kleinlogel H (1985) Spontaneous EEG paroxysms in the rat: effects of psychotropic and alpha-adrenergic agents. Neuropsychobiology 13:206-213.
Manning JP, Richards DA, Leresche N, Crunelli V, Bowery NG (2004) Cortical-area specific block of genetically determined absence seizures by ethosuximide. Neuroscience 123:5-9.
Meeren HK, Pijn JP, Van Luijtelaar EL, Coenen AM, Lopes da Silva FH (2002) Cortical focus drives widespread corticothalamic networks during spontaneous absence seizures in rats. J Neurosci 22:1480-1495.
Mendez MF, Doss RC, Taylor JL, Salguero P (1993) Depression in epilepsy. Relationship to seizures and anticonvulsant therapy. J Nerv Ment Dis 181:444-447.
Nicolelis MA, Baccala LA, Lin RC, Chapin JK (1995) Sensorimotor encoding by synchronous neural ensemble activity at multiple levels of the somatosensory system. Science 268:1353-1358.
Nilsson L, Tomson T, Farahmand BY, Diwan V, Persson PG (1997) Cause-specific mortality in epilepsy: a cohort study of more than 9,000 patients once hospitalized for epilepsy. Epilepsia 38:1062-1068.
O'Donoghue MF, Goodridge DM, Redhead K, Sander JW, Duncan JS (1999) Assessing the psychosocial consequences of epilepsy: a community-based study. Br J Gen Pract 49:211-214.
Polack PO, Charpier S (2006) Intracellular activity of cortical and thalamic neurones during high-voltage rhythmic spike discharge in Long-Evans rats in vivo. J Physiol 571:461-476.
Robertson MM, Channon S, Baker J (1994) Depressive symptomatology in a general hospital sample of outpatients with temporal lobe epilepsy: a controlled study. Epilepsia 35:771-777.
Sarkisova KY, Kulikov MA (2006) Behavioral characteristics of WAG/Rij rats susceptible and non-susceptible to audiogenic seizures. Behav Brain Res 166:9-18.
Sarkisova KY, Midzianovskaia IS, Kulikov MA (2003) Depressive-like behavioral alterations and c-fos expression in the dopaminergic brain regions in WAG/Rij rats with genetic absence epilepsy. Behav Brain Res 144:211-226.
Semba K, Komisaruk BR (1984) Neural substrates of two different rhythmical vibrissal movements in the rat. Neuroscience 12:761-774.
Semba K, Szechtman H, Komisaruk BR (1980) Synchrony among rhythmical facial tremor, neocortical 'alpha' waves, and thalamic non-sensory neuronal bursts in intact awake rats. Brain Res 195:281-298.
Shaw FZ (2004) Is spontaneous high-voltage rhythmic spike discharge in Long Evans rats an absence-like seizure activity? J Neurophysiol 91:63-77.
Shaw FZ (2005) Role of high-voltage rhythmic spikes discharges in Long Evans rats evaluated by antiepileptic drugs and flash stimulation. Soc eurosci Abstr P.173.17.
Shaw FZ, Liao YF (2005) Relation between activities of the cortex and vibrissae muscles during high-voltage rhythmic spike discharges in rats. J Neurophysiol 93:2435-2448.
Shaw FZ, Lai CJ, Chiu TH (2002) A low-noise flexible integrated system for recording and analysis of multiple electrical signals during sleep-wake states in rats. J Neurosci Methods 118:77-87.
Shaw FZ, Lee SY, Chiu TH (2006) Modulation of somatosensory evoked potentials during wake-sleep states and spike-wave discharges in the rat. Sleep 29:285-293.
Sitnikova E, van Luijtelaar G (2004) Cortical control of generalized absence seizures: effect of lidocaine applied to the somatosensory cortex in WAG/Rij rats. Brain Res 1012:127-137.
Snead OC, 3rd, Depaulis A, Vergnes M, Marescaux C (1999) Absence epilepsy: advances in experimental animal models. Adv Neurol 79:253-278.
Van Luijtelaar EL, Coenen AM (1988) Circadian rhythmicity in absence epilepsy in rats. Epilepsy Res 2:331-336.
van Luijtelaar G, Budziszewska B, Tetich M, Lason W (2003) Finasteride inhibits the progesterone-induced spike-wave discharges in a genetic model of absence epilepsy. Pharmacol Biochem Behav 75:889-894.
van Luijtelaar G, Budziszewska B, Jaworska-Feil L, Ellis J, Coenen A, Lason W (2001) The ovarian hormones and absence epilepsy: a long-term EEG study and pharmacological effects in a genetic absence epilepsy model. Epilepsy Res 46:225-239.
Vanderwolf CH (2000) Are neocortical gamma waves related to consciousness? Brain Res 855:217-224.
Wiest MC, Nicolelis MA (2003) Behavioral detection of tactile stimuli during 7-12 Hz cortical oscillations in awake rats. Nat Neurosci 6:913-914.
Willoughby JO, Mackenzie L (1992) Nonconvulsive electrocorticographic paroxysms (absence epilepsy) in rat strains. Lab Anim Sci 42:551-554.
電子全文 電子全文(本篇電子全文限研究生所屬學校校內系統及IP範圍內開放)
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊
 
系統版面圖檔 系統版面圖檔