臺灣博碩士論文加值系統

蛋白質穩定的需求不只是在實驗室，在工業上的需求也是相當大，但總是苦惱無法用更快速有效的方式在事前得到目標蛋白質的穩定資訊，導致常見到事倍功半的實驗發生。而近年來的研究可以看出蛋白質穩定的成因並不是透個單一因素可以來做說明，曝露出預測單一蛋白質穩定因素工具的不足，因此我們透過實驗室過去發展的蛋白質溫度穩定預測演算法：Temperature Index，再合併蛋白質半衰期穩定預測的：Instability Index，將其發展成Web介面的線上工具，透過兩個焦點在不同蛋白質穩定因素的工具互相配合下，提供使用者更準確的建議，我們更將序列置換的調控功能置入，發展成為一套完整的線上蛋白質穩定預測工具，可應用在蛋白質工程方面做修改分析。

The demand of stable protein is not only in a laboratory , but also in the industry. Always it is hard to get protein stability information in a faster and more effective way before experiments, which leads to the fact that experiments often bring half of the result with twice or much more effort. Research in recent years shows that a single factor is not the only cause of protein stability. It reveals prediction deficiency with analysis of a powerful tool, therefore, we propose the algorithm that predicts the protein temperature stability developed in the past in the laboratory : Temperature Index, and Instability Index, which predicts the protein stability in protein’s half-life. We program the two algorithms to be an on-line tool, and provide more accurate suggestions to users through the cooperation of two tools focusing on different protein stability factors. Furthermore, we make a Sequence replace function on the tool as well, which can be utilized in the application of amendment analysis in protein engineering.

第一章 諸論
1.1：研究背景與動機................................. 1
1.2：研究目的....................................... 2

第二章 文獻探討
2.1：蛋白質穩定的重要性............................. 3
2.2：蛋白質穩定的研究方向........................... 4

第三章 研究方法與結果
3.1：建立運作的環境................................. 6
3.2：重建Predict Melting Temperature工具............ 7
3.3：驗證Predict Melting Temperature結果............17
3.4：重建Predict Stability..........................18
3.5：確認Temperature Index和Instability Index工.....19
3.6：建立網站服務
3.6.1：建立Protein Stability Tuner工具..............26
3.6.2：建立Discover Demark Rules工具................27
3.6.3：系統配置.....................................28
3.7：網頁操作說明
3.7.1：BioTuner網站地圖.............................29
3.7.2：Protein Stability Tuner網頁說明..............30
3.7.3：Protein Stability Tuner進階使用..............36
3.7.4：Discover Demark Rules工具....................42

第四章 結論與未來研究方向...........................52

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