臺灣博碩士論文加值系統

由於人類序列在DNA轉錄的過程中，發生不可避免的重新排列組合，將會影響蛋白質的胺基酸序列表現，進而成為遺傳工程上重要的訊息，在這些序列所衍生出的染色體變異問題中，我們利用生物資訊方法，從dbEST資料庫取得五百多萬條人類的EST序列，利用轉位演算法，將其原本低分的序列裁切成二條新序列後，重新定位到基因體上，找到可能發生的刪除序列有1761條，反轉序列有1963條，以及轉位序列有37735條；此研究報告能讓生物資訊相關領域的學者與研究人員都能在更低的成本之下，得到所需求的資訊

Since there occurs an unavoidable rearrangement of the human DNA sequence during its transcription, such a rearrangement will influence the expression of the protein amino acid sequence, which becomes important information on genetic engineering. To approach the problem of chromosome variation derived from the sequence, we utilize bioinformatics procedures to search out chromosome sequence variations, deletion include 1761,inversion include 1963,translocation include 37735 sequences. It can provide the scholars and the researchers of related bioinformatics a more efficient and economic way to obtain desired information on chromosomal variation.

目錄

中文摘要 i
ABSTRACT ii
致謝 iii
目錄 iv
圖目錄 vi
表目錄 vii
第一章 緒論 1
1.1 動機說明 1
1.2 理論背景 3
1.3 研究目的 4
1.4 研究方法與架構 5
1.5 論文章節摘要 8
第二章 相關文獻回顧 9
2.1生物相關研究 9
2.2比對序列軟體 16
2.3低份序列分析方法相關研究 18
第三章 研究方法 22
3.1 裁切位之選擇 22
3.2 分析染色體變異型態 27
第四章 結果 31
4.1 刪除序列 31
4.2 反轉序列 34
4.3 轉位序列 35
第五章 討論 38
參考文獻 39
附錄A 41
附錄B 49
附錄C 57
附錄D 63
附錄E 75

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