臺灣博碩士論文加值系統

此論文我們報告了FePt奈米粒子的製備、相轉換程序、與生物分子的結合及其在微波響應等成果。程序上我們利用化學還原法，製備出約2-3 nm均勻分散的FePt奈米粒子。由於水溶性的磁性奈米粒子在部分生醫領域有廣泛的應用，本研究利用相轉換的程序使FePt奈米粒子從油溶性轉為水溶性，藉由FT-IR及XPS的結果檢驗奈米粒子的表面官能基變化，觀察到粒子表面在相轉換前後的特徵型態及鍵結方式。經由相轉換的FePt水溶性奈米粒子，我們嘗試與目標生物分子結合作為藥物釋放的前驅物，此研究中選用的是streptavidin和biotin，利用protein-label的方式檢驗FePt奈米粒子和分子結合的定量分析。

在此研究中我們亦嘗試將製備的FePt奈米粒子置於水溶液中觀察其介電係數的變化以作為其在生醫應用的參數，藉由我們設計的水溶液量測系統，可以精確量測到FePt在水溶液當中在不同頻率下(45 MHz-50 GHz)的介電係數。

In this thesis, we reported the synthesis of FePt nanoparticles, the phase transfer process, the conjugation of FePt nanoparticles with biomolecules, and the microwave response of FePt solutions. We have synthesized 2-3 nm monodispersed hydrophobic FePt nanoparticles by chemical reduction method. Nevertheless, the water-based nanoparticles have been more widely used in biomedical applications. We utilized the phase transfer process to transfer FePt nanoparticles from hydrophobic to hydrophilic. The surface modified FePt nanoparticles were examined by FT-IR and XPS. The surface characteristics and binding forms of different phase nanoparticles were observered. The ligand exchanged FePt nanoparticles were water-based which had been conjugated with the target biomolecules, streptavidin and biotin, to form the precursors for drug delivery. The quantitative analysis of the conjugation of FePt nanoparticles with biomolecules was examined by protein-label method.

To be an important parameter for the biomedical applications, the effective permittivity of these FePt nanoparticles filled in aqueous solution was measured by using an open-ended coaxial line technique. Their data detected from 45 MHz to 50 GHz through their microwave responses provided a very promising method of analysis of nanoparticles in solution for bio-applications.

目錄
中文摘要……………………………………………………………………………Ⅰ
英文摘要……………………………………………………………………………Ⅱ
目錄…………………………………………………………………………………Ⅲ
表目錄………………………………………………………………………………Ⅵ
圖目錄………………………………………………………………………………Ⅶ

第一章 緒論………………………………………………………………………1
1.1奈米技術在生醫的應用…………………………………………………………2
1.2奈米材料的性質…………………………………………………………………7
1.2.1 小尺寸效應………………………………………………………………7
1.2.2 表面效應…………………………………………………………………7
1.2.3 量子尺寸效應……………………………………………………………8
1.3磁性奈米粒子的特性……………………………………………………………10
1.3.1單一磁區現象………………………………………………………………10
1.3.2矯頑場上升現象……………………………………………………………12
1.3.3超順磁效應…………………………………………………………………13
1.3.4表面電子自旋不規則………………………………………………………14
1.3.5材料相變化…………………………………………………………………16
1.3.6表面磁異向性能……………………………………………………………16
1.4磁性奈米粒子在生化的應用……………………………………………………18

第二章 文獻回顧…………………………………………………………………22
2.1奈米粒子的製備…………………………………………………………………23
2.1.1 物理方法…………………………………………………………………24
2.1.2 化學方法…………………………………………………………………27
2.2 FePt奈米粒子的介紹…………………………………………………………34
2.2.1 FePt奈米粒子的特性……………………………………………………34
2.2.2 FePt奈米粒子的製備……………………………………………………36
2.2.3 FePt奈米粒子在生醫的應用………………………………………………39
2.3生物分子的介紹…………………………………………………………………53
2.3.1 Streptavidin……………………………………………………………53
2.3.2 Biotin……………………………………………………………………55
2.3.3 Streptavidin-Biotin的複雜結構……………………………………57
2.4生物結合系統與偵測分析的介紹…………………………………………………59
2.4.1 Surface Modification of Nanoparticles…………………………59
2.4.2 Zero-Length Cross-linkers……………………………………………61
2.4.3 酵素免疫分析法………………………………………………………………62
2.5奈米粒子水溶液的微波量測………………………………………………………64

第三章 水溶性FePt磁性奈米粒子的製備…………………………………………65
3.1研究動機與目的……………………………………………………………………66
3.2實驗設計……………………………………………………………………………67
3.2.1製備油溶性FePt奈米粒子…………………………………………………67
3.2.2製備水溶性FePt奈米粒子…………………………………………………68
3.3實驗藥品與儀器……………………………………………………………………70
3.3.1藥品……………………………………………………………………………70
3.3.2儀器……………………………………………………………………………71
3.4實驗步驟……………………………………………………………………………72
3.4.1製備油溶性FePt磁性奈米粒子………………………………………………72
3.4.2製備水溶性FePt磁性奈米粒子………………………………………………73
3.5結果與討論…………………………………………………………………………75
3.5.1 FePt奈米粒子的晶格結構……………………………………………………75
3.5.2 FePt奈米粒子的粒徑分布……………………………………………………76
3.5.3FePt奈米粒子的成分分析……………………………………………………80
3.5.4 FePt奈米粒子的磁性性質…………………………………………………80
3.5.5 FePt奈米粒子的表面特徵…………………………………………………81

第四章 FePt磁性奈米粒子與生物分子的結合……………………………………88
4.1研究動機與目的……………………………………………………………………89
4.2實驗設計……………………………………………………………………………90
4.2.1 Nanoparticles Conjugate with Proteins………………………90
4.2.2分析方法………………………………………………………………………91
4.3實驗藥品與儀器……………………………………………………………………92
4.3.1藥品……………………………………………………………………………92
4.3.2儀器……………………………………………………………………………92
4.4實驗步驟……………………………………………………………………………93
4.4.1將生物分子修飾在水溶性FePt奈米粒子表面………………………………93
4.4.2檢驗奈米粒子與生物分子的結合……………………………………………94
4.5結果與討論…………………………………………………………………………96
4.5.1 定性分析……………………………………………………………………96
4.5.2 定量分析……………………………………………………………………105

第五章 FePt磁性奈米粒子的微波響應……………………………………………111
5.1研究動機與目的……………………………………………………………………112
5.2實驗方法……………………………………………………………………………113
5.3結果與討論…………………………………………………………………………114
5.3.1去離子水的介電性質在理論與實際量測的結果……………………………114
5.3.2 FePt磁性奈米粒子的微波響應………………………………………………114

第六章 研究結論……………………………………………………………………120
第七章 參考文獻……………………………………………………………………123

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