臺灣博碩士論文加值系統

本研究主要包含聚乙二醇-鹽兩水相之三成分液液相平衡數據量測，與蛋白質在聚乙二醇-鹽兩水相之分佈係數的量測。三成份液液相平衡數據是利用折射儀量測PEG濃度，與利用原子吸收光譜量測鈉離子含量以推算鹽類濃度。水溶性聚合物選用PEG4000，三種鈉鹽選取硫酸鈉、硫酸氫鈉和氯化鈉。溫度選定在15℃、20℃、30℃，壓力在常壓下。蛋白質分佈係數的測量部分，以PEG4000-硫酸鈉水兩相系統，蛋白質選用雞卵蛋白。溫度在15℃、20℃、30℃，壓力在常壓下。
實驗結果顯示，含硫酸鈉、硫酸氫鈉系統之相平衡行為呈現溫度相依之效應，氯化鈉則觀看不到相分離現象。蛋白質溶解度隨鹽濃度的增加而增加。相平衡所得實驗數據則以Extended UNIQUAC model 作關聯計算。

The subject of the present study include the measurement of liquid-liquid equilibrium data and Partition Coefficient of protein in polyethylene glycol –salt –aqueous two phase system. The PEG concentration of liquid-liquid equilibrium data were measured with Refractometer and the salt concentration were measured with Atomic Absorption spectrophotometer. The polymer of glycol was PEG4000,and the salts were sodium sulfate、sodium bisulfate and sodium chloride. Experiment temperatures were chosen at 15℃、20℃、30℃.pressure in normal. The Partition Coefficient of protein was measured with PEG4000- sodium sulfate–aqueous two phase system, protein was Albumin form chicken egg Experiment temperatures were chosen at 15℃、20℃、30℃.pressure in normal.
According to experimental data, the phase behavior of sodium sulfate and sodium bisulfate system depend on temperature. Sodium chloride system can not spread to two phase. The Partition Coefficient of protein was increase with the tie line length. All of experimental data will correlate by Extended UNIQUAC model.

中文摘要……………………………………………………………………………. I
英文摘要…………………………………………………………………………….II
誌謝………………………………………………………………………………….III
目錄………………………………………………………………………………….IV
表目錄…………………………………………………………………………… …VI
圖目錄……………………………………………………………………………….VII
第一章 緒論 1
1.1水兩相相平衡及蛋白質分佈係數 1
1.2水兩相系統的模式與文獻回顧 4
1.3本研究的動機 10
1.4本文各章重點 10
第二章 液液相平衡實驗量測 11
2.1相平衡量測設備 11
2.2化學藥品 11
2.3檢量線的製作 12
2.4平衡曲線及縛線量測 13
2.5半經驗式關連 16
第三章 蛋白質分佈係數量測 36
3.1蛋白質濃度與分佈係數量測裝置 36
3.2化學藥品 36
3.3蛋白質濃度檢量線的製作 37
3.4蛋白質溶解度的量測 37
3.5蛋白質於兩水相系統之分佈係數量測 38
第四章 液液相平衡的計算 46
4.1 活性係數模式 46
4.2最適化計算 50
第五章 結論 61
參考文獻 64
附錄A 66
符號說明 70

1.Albertsson, P.A., Wiley-Interscience, New York, (1986).
2.Wu, Y.T., Zhu, Z.Q., Lin, D.Q., Mei, L.H., Fluid Phase Equilibria, Vol. 121, 125-139, (1996).
3.Gao, Y. L., Peng. Q. H., Li. Z. C., Li, Y. G.., Fluid Phase Equilibria, Vol. 63 , 157-171, (1991).
4.Gao, Y.L., Peng, Q.H., Li, Z.C., Li, Y.G.., Fluid Phase Equilibria, Vol. 63 , 173-182, (1991).
5.陳文章，周士農，臺北科技大學學報，第三十四之二期，民國九十年。.
6.Kuboi, R., Mruki,T., Tanaka, H., Komasawa, I., Journal of Fermenation And Bioengineering, Vol.78, 431-436, (1994).
7.Osseo-Asare, K., Zeng, X., Int. J. Miner. Process, Vol. 58, 319-330, (2000).
8.Jean, V., C. P., M. A.,C. G., Y van L., Int. J. Pharmaceutics, Vol. 176,241-249, (1999)..
9.Deirdre, O. C., Anne M.H., Owen I. C., Int. J. Pharmaceutics, Vol. 235,193-205, (2002).
10.Walter, H., Brooks, K.D., Ficher, D., Academic Press, New York,(1985).
11.Jose, C. M., Barbara, A. A., Juan,A. A., J. Chromat. B, Vol. 711, 285-293, (1998).
12.Sylvie de B., Bene. Le. B., Jon., H., Andrew, L., J. Chromat. B, Vol. 711, 19-29, (1998).
13.J. H. Han, Lee C.-H., Colloids and Surfaces B: Biointerfaces, Vol. 9,109-116, (1997).
14.Nina, B., Eric, C., Jean., L., Mathias, U.,Andres, V., Per-A. N., J. Biotechnology, Vol. 79, 161-172, (2000).
15.Rogers, R. D., Bord., A. H., Bauer, C.B., Sep. Sci. Technol., Vol. 28, 1091-1126,(1993).
16.Ananthapadmana, K.P., and Gooddard, E.D., US Patents 47, 225,358, (1987).
17.Chaiko, D. J., Mensah-Biney, R., Mertz, C. J., Rollins, A., Sep. Sci. Technol., Vol. 28, 765-780, (1993)
18.Chaiko, D. J., Vojta, Y., Takeuchi, M., Sep. Sci. Technol., Vol. 30, 1123-1137, (1995).
19.尤明隆，化工技術，第七卷第八期，200-209，民國八十八年。
20.Gaube, J. ,Peennig A. ,Stumpf M., Fluid Phase Equilibria, Vol. 83, 465-373, (1993).
21.H. Cabezas, Jr.J.D. Evans, J.D., Szlag, D.C., Fluid Phase Equilibria, Vol. 53, 453-462, (1989).
22.Zafarani-Moattar, M. T., Gasemi, J., Fluid Phase Equilibria, Vol. 198, 281-291, (2002).
23.Haynes, C.A., Blanch, H.W., Prausnitz, J.M., Fluid Phase Equilibria, Vol. 53 , 463-474, (1991).
24.Voros, N., Proust, P., Fredenslund, A., Fluid Phase Equilibria, Vol. 90 , 333-353, (1993).
25.Zafarani-Moattar, M. T., Sadeghi, R., Fluid Phase Equilibria, Vol. 203, 177-191, (2002)..
26.Peng, Q., Li, Z., Li, Y., Fluid Phase Equilibria, Vol. 95 , 341-357, (1994)..
27.Van Bochove G. H., Krooshof G.. J. ,P.,de Loos T. W., Fluid Phase Equilibria, Vol. 171, 45-58, (2000).
28.Wu, Y.T., Lin, D.Q., Zhu, Z.Q., Fluid Phase Equilibria, Vol. 147 , 25-43, (1998).
29.Wu, Y. T., Zhu, Z. Q., Lin, D. Q., Li, M., Fluid Phase Equilibria, Vol. 154 , 109-122, (1999).
30.Zaslavsky, B. Y., New York, Marcel Dekker Inc., Chapter 10, (1995).
31.van Berlo, M., Luyben, K. Ch.A.M., van der Wielen, L. A.M., Journal of Chromat. B, Vol. 711, 61-68, (1998).
32.Ho-Gutierrez, I. V., Cheluget, E.L., Vera, J. H., Weber, M.E., J. Chem. Eng. Data, Vol. 39, 245-248, (1994).
33.Hammer, S., Pfennig, A., Stumpf, M., J. Chem. Eng. Data, Vol. 39, 409-413, (1994)..
34.Graber, T.A., Taboada, M.E., J. Chem. Eng. Data, Vol. 45, 182-184, (2000).
35.Gonzalez-Tello, P., Camacho, F., Blazquez, G., Alarcon, F.J., J. Chem. Eng. Data, Vol. 41, 1333-1336, (1996).
36.Da Silva, L.H.M., Coimbra, J. S. R.,Meirelles, A. J. de A., J. Chem. Eng. Data, Vol. 42, 398-401, (1997).
37.Zafarani-Moattar, M. T., Nehrdad, A., J. Chem. Eng. Data, Vol. 45, 182-184, (2000).
38.Taboada M. E., Rocha O. A., Graber T. A., J. Chem. Eng. Data, Vol. 46, 308-311, (2001).
39.Kaj Thomsen, Ph.D. Thesis, Department of Chem. Eng. Tech. University, Denmark, (1997).
40.Dlamond n D., Hsu James T., J. Chem. Eng. Data, Vol. 35, 420-423, (1990).
41.Steven M. Snyder, Kenneth D. Cole, Davld C. Szlag, J. Chem. Eng. Data, Vol. 37, 268-274, (1992).
42.Rogerio A. G. Se , Martýn Aznar, J. Chem. Eng. Data, Vol. 47, 1401-1405, (2002).
43.Wu Y. T., Zhu Zi-Q., Mei L. H., J. Chem. Eng. Data, Vol. 41, 1032-1035, (1996).
44.Graber T. A.,Gallequillos H., J. Chem. Eng. Data, Vol. 47, 174-178, (2002).
45.Anton Pear, Instruction Manual,Xdlb10
46.季正偉 “聚乙烯乙二醇-鹽水兩相相平衡研究”,碩士論文
國立台北科技大學,民國九十四年一月。
47.Qinhua Peng, Zongcheng Li, Yigui Li Fluid Phase Equilibria, Vol. 107 , 303-315, (1995).
48. Proceedings ICUMSA P. 151(1974) + correction (1978)