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研究生:張耀元
研究生(外文):Chang, Yao-Yuan
論文名稱:表沒食子兒茶素沒食子酸酯對於老化老鼠血液流變異常之改善評估
論文名稱(外文):Improving Effects of Epigallocatechin-3-gallate on Hemorheological abnormality in Aging Guinea Pigs
指導教授:劉得任劉得任引用關係
指導教授(外文):Liu, Der-Zen
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:生物醫學材料研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:76
中文關鍵詞:表沒食子兒茶素沒食子酸酯血液流變
外文關鍵詞:EGCGHemorheological abnormality
相關次數:
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表沒食子兒茶素沒食子酸酯(Epigallocatechin-3-gallate,EGCG)是綠茶中最豐富的兒茶素,同時在動物模型中具有顯著的抗氧化能力。在本研究中, 我們選擇了自然老化24個月大的天竺鼠做為動物實驗模型,分別以10、20、30 mg/kg/day EGCG (分別是實驗組A、B、C) 餵養28 天後,來探討EGCG對於老化天竺鼠異常血液流變值的效用。

結果顯示,對於經口服用EGCG 10mg/kg/day二十八天的天竺鼠(A組)的血液流變參數與控制群D比較(EGCG 0mg/kg/day),並沒有明顯的變化。但是,當EGCG的量到達20mg/kg/day(B組),雖然在他們的紅血球聚集度、血液黏度和血液黏彈性上仍沒有顯著的差異,但是紅血球膜的MDA值開始顯著減少(p<0.05),似乎表示了紅血球變形度的上升和紅血球流抗阻力的減少。而且,在給予老化天竺鼠EGCG 30mg/kg/day (高劑量)後,不論是血液黏度、血液黏彈性、紅血球聚集或紅血球變形度等血液流變參數,都有明顯的改善。
Epigallocatechin-3-gallate (EGCG), the most abundant catechin in green tea, has been shown to have pronounced anti-oxidative activity in animal models. In our study, we selected 24 months old aging guinea pigs as the experimental model, fed with diet containing 10, 20 and 30mg/kg/day of EGCG (experimental groups A, B, and C) for 28 days, respectively, in order to investigate the effects of EGCG on the abnormal hemorheological parameters of the aging guinea pigs.

The results showed that blood biochemical parameters of the aging guinea pigs remained unaffected by orally given EGCG for 28 days compared to the controls groups D ( 0mg/kg/day of EGCG). The hemorheological results indicate that aging guinea pigs fed with low dose of EGCG (10mg/kg/day) did not have significant changes in hemorheological parameters compared with the control group. However, as the EGCG content reached to 20mg/kg/day, though there were still no prominent alteration in their erythrocyte aggregation, blood viscosity and blood viscoelasticity, but, the erythrocyte membrane MDA levels of aging guinea pigs started to exhibit significantly reduced (P<0.05) that seems to be responsible for the rise in erythrocyte deformability and the reduction in erythrocyte flow resistance. Moreover, the feeding of EGCG at 30mg/kg/day (high dose), significantly improved most of abnormal hemorheological parameters whether blood viscosity, blood viscoelaticity, erythrocyte aggregation or erythrocyte deformability.
中文摘要
英文摘要
圖表索引
縮寫簡稱
第一章、緒論
1-1 前言
1-2 血液流變
1-2-1血液流變的定義
1-2-2血液流變對於生理之意義
1-2-3血液流變與疾病的關係
1-3 血液動力學參數
1-3-1 黏度(viscosity)
1-3-2 影響血漿及血液黏度的因素
1-3-3 黏彈流體之介紹
1-3-4 影響血液黏彈性的因素
1-4 紅血球變形度
1-4-1 紅血球膜
1-4-2 紅血球變形性生理及病理學之意義
1-4-3 影響紅血球變形度之因素
1-4-4 紅血球變形度測試
1-5 紅血球聚集性
1-5-1 紅血球聚集度測試方法
1-6 紅血球膜自由基反應
1-6-1 自由基
1-6-2 自由基造成的傷害
1-6-3 紅血球膜脂質過氧化的反應機制
1-6-4 紅血球脂質膜過氧化對紅血球之影響
1-6-5 紅血球膜脂質過氧化測試
1-7 纖維蛋白原對血液的影響
1-7-1纖維蛋白原(fibrinogen)
1-7-2 纖維蛋白原的生合成
1-7-3 纖維蛋白原的功能
1-8表沒食子兒茶素沒食子酸酯(EGCG)
1-8-1 EGCG的結構
1-8-2 EGCG的藥理作用
1-8-3 EGCG的副作用
1-9 研究目的
第二章、材料及方法
2-1 實驗設備
2-1-1 黏度計
2-1-2 紅血球變形及聚集測量儀(LG-B-190型)
2-2 實驗材料
2-3 動物模型Animals
2-4 血液生物化學值的測量
2-5 血液流變參數測量
2-5-1 血漿及血液黏度
2-5-2 血液黏彈性
2-5-3 紅血球細胞膜MDA分析
2-5-4 紅血球流抗阻力
2-5-5 紅血球變形度
2-5-6 紅血球聚集度
2-5-7 紅血球堅硬度與血液氧氣運輸效率
2-6 統計分析
第三章、結果
3-1 EGCG對生化與血液參數的影響
3-2 EGCG對血液流變參數的影響
3-3 EGCG對紅血球細胞的影響
第四章、討論
第五章、結論參考文獻
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