臺灣博碩士論文加值系統

本研究利用仙草癒合組織與十種唇形花科植物，比較其迷迭香酸與熊果酸含量、抗氧化能力與酪胺酸酶抑制能力差異。在仙草癒合組織培養累積迷迭香酸含量中，使用MS培養基添加4 mg/l NAA與0.5 mg/l KIN 於培養葉部及莖段培植體中，可得到最高的迷迭香酸含量，分別為99.31±0.97 與112.98±0.05 mg/g DW，此結果與田間之彩葉草（紅葉）之花序迷迭香酸含量相當。於相同液態培養基中添加5 g/l PVP進行懸浮培養，可將迷迭香酸含量提升至129.61±3.24 mg/g DW。而在熊果酸含量上，而在十種唇形花科植物中，溪黃草葉部有最高的熊果酸含量，為10.89±0.21 mg/g DW；仙草癒合組織略低於田間仙草的含量，為0.97±0.09 mg/g DW，介於十種唇形花科植物之中，但高於in vitro植株。在DPPH 清除能力中，田間唇型花科植物之各部位比較為花序>葉>莖。而仙草癒合組織與田間田間唇型花科植物在系統濃度為100 μg/ml 下，其清除能力均在90 % 以上。甲醇萃取物不論在何種系統濃度下均較水萃取物DPPH 清除能力為強。在酪胺酸酶抑制能力方面，仙草癒合組織或是田間取得之仙草，其酪胺酸酶抑制能力均不明顯，在十種唇形花科植物中，彩葉草（紅葉）、廣防風與金錢薄荷的酪胺酸酶抑制能力較佳。

The rosmarinic and ursolic acid content, antioxidant and tyrosinase inhibition activity in Mesona procumbens callus and ten kinds of Lamiaceae plants were examed in this study. The highest content of rosmarinic acid（112.98±0.05 mg/g DW）was obtained from the callus induced from stem explant of Mesona procumbens on MS media supplemented with 4 mg/l NAA or 0.5 mg/l KIN .This result was the same as the wild inflorescence of Solenostemon scutellarioides (red leaf). The contant of rosmarinic acid in cells raised to 129.61±3.24 mg/g DW when cells transfered to the same composition of liquid medium plus 5 g/l PVP. The content of ursolic acid in callus of Mesona procumbens（0.97±0.09 mg/g DW）was less than most other plants of Lamiaceae（1~10 mg/g DW）. The comparison of DPPH radical scavenging activity with different organs, inflorescence has the best radical scavenging activity, leaf second and stem third. The DPPH radical scavenging activity of methanol and water extract from callus of Mesona procumbens lower than inflorescence of Lamiaceae, and between the leaf and stem extract. Under 100 μg/ml of extract from callus Mesona procumbens and Lamiaceae plants,the DPPH radical scavenging activity were higher than 90 %. The average of DPPH radical scavenging activity of methanol extract was higher than water extract from all of the tested plants. The highest tyrosinase inhibition activity was expressed in Solenostemon scutellarioides（red leaf）. Neither callus nor wild plant of Mesona procumbens had obvious activity of tyrosinase inhibition.

中文摘要.................................................................i
ABSTRACT……………………………………………………………ii
目錄……………………………………………………………………iv
LIST OF TABLES………………………………………………………ix
LIST OF FIGURES…………………………………………………….xiv
縮寫字………………………………………………………………..xvii
一、前人研究……………………………………………………………1
1.1唇形花科植物特徵………………………………………………….1
1.2 十種台灣常見唇形花科植物簡介.................................................. 1
1.3 迷迭香酸之介紹 ………………………………………………...11
1.3.1 迷迭香酸之基本化學性質........................................................11
1.3.2 迷迭香酸之生合成路徑............................................................11
1.3.3 迷迭香酸之生理活性................................................................14
1.3.4 田間植株迷迭香酸含量...........................................................15
1.3.5 利用植物組織培養生產迷迭香酸............................................18
1.4 熊果酸之介紹.................................................................................21
1.4.1 熊果酸之基本化學性質............................................................21
1.4.2 熊果酸之生理活性....................................................................21
1.4.3 唇形花科植物之熊果酸含量....................................................21
1.4.4利用植物組織培養生產熊果酸.................................................23
1.5 抗氧化力.........................................................................................27
1.6 酪胺酸酶抑制能力.........................................................................30
1.6.1 黑色素的形成機轉....................................................................30
1.6.2 酪胺酸酶的性質........................................................................32
1.6.3 酪胺酸酶活性的抑制................................................................32
1.6.4 唇形花科植物酪胺酸酶活性抑制能力....................................33
二、材料與方法......................................................................................35
2.1 材料來源.........................................................................................35
2.2仙草癒合組織的誘導.......................................................................35
2.3仙草細胞懸浮培養..........................................................................40
2.4 樣品之乾燥製備.............................................................................40
2.5 熊果酸與迷迭香酸之萃取.............................................................40
2.6 熊果酸之分析.................................................................................40
2.7迷迭香酸之分析..............................................................................41
2.8 DPPH清除能力測定.......................................................................41
2.9 水萃取物對酪胺酸酶活性抑制之測定.........................................44
2.10 標準品...........................................................................................45
2.11 統計分析.......................................................................................45
三、結果..................................................................................................46
3.1 仙草癒合組織培養與迷迭香酸含量.............................................46
3.1.1 葉部癒合組織............................................................................46
3.1.2 莖段癒合組織............................................................................53
3.1.3 仙草細胞懸浮培養....................................................................61
3.2仙草癒合組織與十種唇形花科植物之迷迭香酸含量之比較......63
3.2.1仙草癒合組織迷迭香酸含量......................................................63
3.2.2十種唇形花科植物之各器官迷迭香酸含量..............................63
3.3仙草癒合組織與十種唇形花科植物之熊果酸含量之比較…......65
3.3.1仙草癒合組織熊果酸含量..........................................................65
3.3.2十種唇形花科植物各器官迷迭香酸含量..................................65
3.4仙草癒合組織與十種唇形花科植物之自由基清除能力之比較...68
3.4.1甲醇萃取物DPPH清除能力.....................................................68
3.4.1.1仙草癒合組織甲醇萃取物....................................................68
3.4.1.2十種唇形花科植物之各器官甲醇萃取物............................68
3.4.2水萃取物DPPH清除能力.........................................................74
3.4.2.1仙草癒合組織水萃取物........................................................74
3.4.2.2十種唇形花科植物之各器官水萃取物................................74
3.5仙草癒合組織與十種唇形花科植物酪胺酸酶抑制能力之比較.79
3.5.1仙草癒合組織............................................................................79
3.5.2十種唇形花科植物之不同部位................................................79
四、討論...................................................................................................82
4.1 仙草組織培養與迷迭香酸含量.....................................................82
4.1.1 葉部癒合組織............................................................................82
4.1.2 莖段癒合組織............................................................................83
4.1.3 仙草細胞懸浮培養....................................................................83
4.2 仙草癒合組織與十種唇形花科植物之迷迭香酸含量之比較.....84
4.3 仙草癒合組織與十種唇形花科植物之熊果酸含量之比較.........85
4.4 仙草癒合組織與十種唇形花科植物之DPPH清除能力之比較.87
4.4.1十種唇形花科植物各部位器官之DPPH清除能力.................87
4.4.2 不同萃取相DPPH清除能力之比較........................................88
4.4.3 不同器官DPPH清除能力之比較.............................................88
4.5 仙草癒合組織與十種唇形花科植物酪胺酸酶抑制能力之比較.88
4.6 各種不同來源之仙草比較.............................................................89
4.6.1 迷迭香酸含量比較....................................................................90
4.6.2 熊果酸含量比較………………………………………………90
4.6.3 DPPH清除能力比較..................................................................92
4.6.4 酪胺酸酶抑制力........................................................................94
五、結論..................................................................................................98
六、參考文獻..........................................................................................100

全國中草藥匯編. 1983. 人民衛生出版社. 四川. p. 15-860

李幸祥. 1999. 台灣藥草事典(二). 旺文社股份有限公司. 台北. p. 114-115

李學勇. 1987. 基礎植物學(下冊). 國立編譯館. 台北. p. 1423

洪千雅. 2001. 仙草抗氧化機能性之研究. 國立中興大學食品科學研究所. 博士論文.

姜金龍. 1999. 台灣野生仙草種內變異之研究. 國立中興大學農藝學系. 博士論文.

康兆星. 2003. 植物的風華. 鴻源彩色印刷製版股份有限公司. 台北. p: 134-200

梁靖宗. 2005. 羊肚菌與蜜環菌之抗氧化性質分析. 大同大學生物工程研究所. 碩士論文.

張川虎. 2004. 中草藥對抑制黑色素生合成與抗氧化活性之研究. 中國醫藥大學中國藥學研究所. 碩士論文.

張博仁. 2003. 仙草（Mesona procumbens）組織培養及其細胞萃取物抗氧化力之研究. 大同大學生物工程研究所. 碩士論文.

陳威丞. 2004. 兼具酪胺酸酶抑制力及抗氧化力中藥材之篩選. 大同大學生物工程研究所. 碩士論文.

黃增泉. 1999. 植物分類學：台灣維管束植物科誌. 南天書局有限公司. 台北. p. 360

楊舒婷. 2000. 利用牛舌草植物懸浮細胞培養生產迷迭香酸之研究. 國立台灣大學農業化學研究所. 碩士論文

劉伯康、陳惠英、顏國欽. 1999. 十種傳統食用植物甲醇萃取物抗氧化力之研究. 中國農業化學會誌 37: 105-116

鄭如娟. 2003. 利用Rosmarinus officinalis 懸浮細胞生產迷迭香酸之研究. 國立台灣大學農業化學研究所. 碩士論文

鄭涵云. 2004. 三十一種草藥之美白、抗氧化及抑菌性質之探討. 大同大學生物工程研究所. 碩士論文.

薛聰賢. 1989. 台灣花卉實用圖鑑（二）. 薛氏園藝有限公司出版部. 彰化. p. 60

Abe F., T. Yamauchi, T. Nagao, J. Kinjo, H. Okabe, H. Higo and H. Akahane. 2002. Ursolic acid as a trypanocidal constituent in rosemary. Biol. & Pharmaceut. Bull. 25: 1485-1487

Bilińska B. 2001. On the structure of human hair melanins from an infrared spectroscopy analysis of their interactions with Cu2+ ions. Spectrochim. Acta Part A 57:2525-2533

Bolta Z., D. Baricevic and P. Raspor. 2003. Biomass segregation in sage cell suspension culture. Biotech. Lett. 25: 61-65

Branen A. L. 1975. Toxicology and biochemistry of butylated hydroxyl anisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52: 59-63

Césarini J. P., M. Despiney and I. N. Sem. 1996. Melanins and their possible roles through biological evolution. Adv. Space Res. 18:35-40.

Chen H., F. Chen, Y. L. Zhang and J. Y. Song. 1999. Production of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza. J. Ind. Microbiol. & Biotech. 22: 133-138

Chen J. H., Z. H. Xia and R. X. Tan. 2003. High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens. J. Pharmaceut. and Biomed. Anal. 32: 1175-1179

D’Amelio, F. S. 1999. Botanicals, a phytocosmetic desk reference. CRC Press, London p. 361

De-Eknamkul W. and B. E. Ellis. 1984. Rosmarinic acid production and growth characteristics of Anchusa officinalis cell suspension cultures. Planta Med. 51: 346-350

De-Eknamkul W. and B. E. Ellis. 1985. Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis. Plant Cell Rep. 4: 40-49

Dorman H. J. D., A. Peltoketo, R. Hiltunen and M. J. Tikkanen. 2003. Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem. 83: 255-262

Dorman H. J. D. and R. Hiltunen. 2004. Fe(III) reductive and free radical-scavenging properties of summer savory（Satureja hortensis L.）extracts and subfractions. Food Chem. 88: 193-199

Duncan D. B. 1955. Multiple range and multiple F-test. Biometrics 11:1-24

Es-Saady D., A. Simon, M. Ollier, J. C. Maurizis, A. J. Chulia and C. Delage. 1996a. Inhibitory effect of ursolic acid on B16 proliferation through cell cycle arrest. Cancer Lett. 106: 193-197

Es-Saady D., A. Simon, C. Jayat-Vignoles, A. J. Chulia and C. Delage. 1996b. MCF-7 cell cycle arrested at G1 through ursolic acid, and increased reduction of tetrazolium salts. Anticancer Res. 16: 481-486

Feria-Romero I., E. Lazo, T. Ponce-Noyola, C. M. Cerda-Garcia-Rojas and A. C. Ramos-Valdivia. 2005. Induced accumulation of oleanolic acid and ursolic acid in cell suspension cultures of Uncaria tomentosa. Biotech. Lett. 27: 839-843

Flores-Sanchéz I. J., J. Ortega-López, M. C. Montes-Ilorcasitas and A. C. Ramos-Valdivia. 2002. Biosynthesis of sterols and triterpenes in cell suspension culture of Uncaria tomentosa. Plant Cell Physiol. 43: 1502-1509

Gamborg O. L., R. A. Miller and K. Ojima, 1968. Nutrient requirements of suspension culture of soybean root cells. Exp. Cell Res. 50: 151-158

Georgiev M., A. Pavlov and M. Ilieva. 2004. Rosmarinic acid production by Lavandula vera MM cell suspension：the effect of temperature. Biotech. Lett. 26: 855-856

Gertlowski C. and M. Petersen. 1993. Influence of the carbon source on growth and rosmarinic acid production in suspension cultures of Coleus blumei. Plant Cell, Tiss. Org. Culture 34: 183-190

Gülçin İ., D. Berashvili and A. Gepdiremen. 2005. Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis dence. J. Ethnopharm. 101: 287-293

Hao Z., B. Huang and Y. Wang. 1989. Hypoglycemic effect of oleanolic acid. Zhougguo Yooke Daxue Xuebao 22: 210-212

Hsu H. Y., J. J. Yang and C. C. Lin. 1997. Effects of olanolic acid and ursolic acid on inhibition tumor growth and enhancing the recovery of hematopoietic system postirradiation in mice. Cancer Lett. 111: 7-13

Imida k., S. Fukshima, T. Sgiva, M. Ohtani, K. Nakanishi and N. Ito. 1983. Promoting activities of butylated hydroxyanisole and butylated hydroxytoluene on 2-stage urinary bladder carcinogenesis and inhibition of γ-glutamyl transpeptidase-positive foci development in liver of rats. Carcinogenesis 4: 885-889

Ilieva M. and A. Pavlov. 1996. Rosmarinic acid by Lavandula vera MM cell suspension：phosphorus effect. Biotech. Lett. 18: 913-916

Ilieva M. and A. Pavlov. 1997. Rosmarinic acid by Lavandula vera MM cell-suspension culture. Appl. Microbiol. and Biotech. 47: 683-688

Ilieva M. and A. Pavlov. 1999. Rosmarinic acid production by Lavandula vera MM cell suspension culture：nitrogen effect. World J. Microbiol. & Biotech. 15: 711-714

Janicsák G., I. Máthé, V. MikloHossy-VaHari and G. Blunden. 1999. Comparative studies of the rosmarinic and caffeic acid contents of Lamiaceae species. Biochem. Sys. and Ecol. 27: 733-738

Janicsák G., K. Veres, A. Z. Kakasy and I. Máthé. 2006. Study of the oleanoic and ursolic acid contents of some species of the Lamiaceae. Biochem. Sys. Ecol. 34: 392-396

Jayasinghe C., N. Gotoh, T. Aoki and S. Wada. 2003. Phenolics composition and antioxidant activity of sweet basil（Ocimum bacilicum L.）. J. Agri. Food Chem. 51: 4442-4449

Karam N. S., F. M. Jawad, N. A. Arikat and R. A. Shibli. 2003. Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa. Plant Cell, Tiss. Org. Culture 73: 117-121

Kazuki H., K. Shinichi and Y. Michio. 1996. Natural antioxidants and active oxygen removers in food cosmetics, and pharmaceuticals. Chem. Abs. 124, 200690

Kim H. J., F. Chen, X. Wang and N. Rajapakse. 2005. Effect of chitosan on the biological properties of sweet basil（Ocimum bacilicum L.）. J. Agri. Food Chem. 53: 3696-3701

Kim H. K., S. R. Oh, H. K. Lee and H. Huh. 2001. Benzothiazole enhance the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze. Biotech. Lett. 23: 55-60

Kintzios S., A. Nikolaou, M. Skoula. 1999. Somatic embryogenesis and in vitro rosmarinic acid accumulation in Salvia officinalis and S. fruticosa leaf callus cultures. Plant Cell Rep. 18: 462-466

Kintzios S., O. Makri, E. Panagiotopoulos and M. Scapeti. 2003. In vitro rosmarinic acid accumulation in sweet basil ( Ocimum bacilicum L. ). Biotech. Lett. 25: 405-408

Kintzios S., H. Kollias, E. Straitouris and O. Makri. 2004. Scale-up micropropagation of sweet basil ( Ocimum bacilicum L. ) in an airlift bioreactor and accumulation of rosmarinic acid. Biotech. Lett. 26: 521-523

Komali A. S. and K. Shetty. 1998. Comparison of the growth pattern and rosmarinic acid production in rosemary ( Rosmarinus officinalis ) shoots and genetically transformed callus cultures. Food Biotech. 12: 27-41

Kovatcheva E., A. Pavlov, I. Koleva, M. Ilieva and M. Mihneva. 1996. Rosmarinic acid from Lavandula vera MM cell culture. Phytochem. 43: 1243-1244

Lai L. S., S. T. Chou and W. W. Chao. 2001. Studies on the antioxidative activities of Hsian-tsao（Mesona procumbens Hemsl）leaf gum. J. Agri. Food Chem. 49: 963-968

Larson R. A. 1988. The antioxidants of higher plants. Phytochem. 27 :969-978

Li W., K. Koike, Y. Asada, T. Yoshikawa and T. Nikaido. 2005. Rosmarinic acid production by Coleus forskohlii hairy root cultures. Plant Cell, Tiss. Org. Culture 80: 151-155

Liu Z., D. Wang, S. Wang, S. Ha and P. Li. 1994. Oleanolic acid in decreasing hyperglycemia. Chinese Pharmaceut. J. 29: 725-726

Liu J., Y. P. Liu and C. D. Klaassen. 1995. The effect of Chinese hepatoprotective medcines of experimental liver injury in mice. J. Ethnopharm. 42: 183-191

Marmol V. D., and B. F. Beermann. 1996. Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381:165-168

Mazumder, A., N. Neamati, S. Sunder, J. Schulz, H. Pertz, E. Eich and Y. Pommier. 1997. Curcumin analogs with altered potencies against HIV-1 intergrase as probes for biochemical machenisms of drug action. Euro. J. Med. Chem. 40: 3057-3063

Mimika-Dukic N., B. Bozin, M. Sokovic and N. Simin. 2004. Antimicrobial and antioxidant activities of Melissa officinalis L.（Lamiaceae）essential oil. J. Agri. Food Chem. 52: 2485-2489

Mizukami H., T. Ogawa, H. Ohashi and B. E. Ellis. 1992. Induction of rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures by yeast extract. Plant Cell Rep. 11: 480-483

Mizukami H., Y. Tabira and B. E. Ellis. 1993. Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep. 12: 706-709

Mohagheghzadeh A., M. Shams-Ardakani, A. Ghannadi and M. Minaeian. 2004. Rosmarinic acid from Zataria multiflora tops and in vitro cultures. Fitoterapia 75: 315-321

Morimoto S., Y. Goto and Y. Shoyama. 1994. Production of lithospermic acid B and rosmarinic acid in callus tissue and regenerated plantlets of Salvia miltiorrhiza. J. Nat. Prod. 57: 817-823

Murakami Y., T. Omoto, I. Asai, K. Shimomura, K. Yoshihira and K. Ishimaru. 1998. Rosmarinic acid and related phenolics in transformed root cultures of Hyssopus officinalis. Plant Cell, Tiss. Org. Culture 53: 75-78

Murashige T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiol. Planta. 15: 437:497

Nishino H., A. Nishino, J. Takayasu, A. Iwashima, K. Hirabayashi, S. Iwata and S. Shibata. 1988. Inhibition of the tumor-promoting action of 12-O-tetradacanoy-phorbol-13-acetate by some oleanane-type triterpennoid compounds. Cancer Res. 48: 5210-5215

Ozeki H., I. S., and I. I.. 1997. Chemical characterization of pheomelanogenesis starting from dihydroxyphenylalanine or tyrosinase and cysteine.Effects of tyrosinase and cysteine concentrations and reaction time. Biochim. Biophy. Acta 1336:539-548

Parnham M. J. and K. Kesselring. 1985. Rosmarinic acid. Drugs of the Future 10: 756-757

Pavlov A. and M. Ilieva. 1999. The influence of phenylalanine on accumulation of rosmarinic and caffeic acids by Lavandula vera MM cell culture. World J. Microbio. & Biotech. 15: 397-399

Pavlov A. I., M. P. Ilieva and I. N. Panchev. 2000. Nutrient medium optimization for rosmarinic acid production by Lavandula vera MM cell suspension. Biotech. Prog. 16: 668-670

Petersen M., E. Häusler, J. Meinhard, B. Karwatzki and C. Gertlowski. 1994. The biosynthesis of rosmarinic acid in suspension cultures of Coleus blumei. Plant Cell, Tiss. Org. Culture 38: 171-179

Petersen M. and M. S. J. Simmonds. 2003. Rosmarinic acid. Phytochem. 62: 121-125

Piao L. Z., H. R. Park, Y. K. Park, S. K. Lee, J. H. Park and M. K. Park.
2002. Mushroom tyrosinase inhibition activity on some chromones.
Chem. & Pharmaceut. Bull. 50: 309-311

Rady M. R. and N. M. Nazif. 2005. Rosmarinic acid content and RAPD analysis of in vitro regenerated basil ( Ocimum americanum ) plants. Fitoterapia 76: 525-533

Recio M. C., R M. Giner, S. Manez, J. Gueho, H. R. Julien, K. Hostettmann and J. L. Rios. 1995. Investigations on the steroidal anti-inflammatory activity of triterpenoids from Diospyros leucomelas. Planta Med. 61: 9-12

Santos-Gomes P. C., R. M. Seabra, P. B. Andrade and M. Fernandes-Ferreira. 2002. Phenolic antioxidant compounds produced by in vitro shoots of sage ( Salvia officinalis L. ). Plant Sci. 162: 981-987

Shimada K., K. Fujikawa, K. Yahara and T. Nakamura. 1992.
Antioxidative properties of xanthan on the autoxidation of soybean oil
in cyclodextrin emulsion. J. Agri. Food Chem. 40: 945-948

Singh G. B., S. Singh, S Bani, B. D. Gupta and S. K. Banerjee. 1992. Anti-inflammatory activity of oleanolic acid in rats and mice. J. Pharm. Pharmacol. 44: 456-458

Snyckers F. Q. and T. G. Fourle. 1984. Ulcer prevention and treatment with oleanoic acid. Chem. Abs. 100: 39613k

Su W. W. and F. Lei. 1993. Rosmarinic acid production in perfused Anchusa officinalis culture：effect of inoculum size. Biotech. Lett. 15: 1035-1038

Suh N., T. Honda, H. J. Finaly, A. Barchowsky, C. Williams, N. E. Benoit, Q. W. Xie, C. Nathan, G. W. Gribble and M. B. Sporn. 1998. Novel triterpenoids suppress inducible nitric oxide synthase（iNOS）and inducible cyclooxygenase（COX-2）in mouse macrophages. Cancer Res. 58: 717-723

Tada H., Y. Murakami, T. Omoto, K. Shimomura and K. Ishimaru. 1996. Rosmarinic acid and related phenolics in hairy root cultures of Ocimum bacilicum. Phytochem. 42: 431-434

Taniguchi S., Y. Imayoshi, E. Kobayashi, Y. Takamatsu, H. Ito, T. Hatano, H. Sakagami, H. Tokuda, H. Nishino, D. Sugita, S. Shimura and T. Yoshida. 2002. Production of bioactive triterpenes by Eriobotrya japonica calli. Phytochem. 59: 315–323

Tepe B., D. Daferera, A. Sokmen, M. Sokmen and M. Polissiou. 2005. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller（Lamiaceae）. Food Chem. 90: 333-340

Vasilenko Y. K., V. D. Ponomarev and E. T. Oganesyan. 1981. Comparative study of the hypolipemic properties of triterpenoids. Chemical Abs. 91: 49279r

Vasilenko Y. K., L. I. Lisevitskaya, L. M. Frolova, E. P. Parfenteva, I. V. Skulte, A. Y. Vasilenko, V. V. Kozharskii and V. L. Voskayan. 1982. Hypolopemic properties of triterpenoids. Farmacol. Toxicol. 45: 66-70

Wang H., G. J. Provan and K. Helliwell. 2004. Determination of rosmarinic acid and caffeic acid in aromatic herbs by HPLC. Food Chem. 87: 307-311

Wang J. W., Z. H. Xia, J. H. Chu and R. X. Tan. 2004. Simultaneous production of anthocyanin and triterpenoids in suspension cultures of Perilla frutescens. Enzyme and Microbial Tech. 34: 651–656

Wrzeciono U., I. Malecki, J. Budzianowski, H. Kierylowicz, L. Zaprutko, E. Beimvik and H. Kostepska. 1985. Nitrogenous triterpene derivatives. Part 10; Hemisuccunates of ome derivatives of oleanoic acid ad their antiulcer effects. Pharm. 40: 542-544

Yang R. and K. Shetty. 1998. Stimulation of rosmarinic acid in shoot cultures of oregano ( Origanum vulgare ) clonal line in response to proline, proline analogue, and proline precursors. J. Agri. Food Chem. 46: 2888-2893

Yen G. C. and C. Y. Hung. 2000. Effects of alkaline and heat treatment on antioxidative activity and total phenolics of extracts from Hsian-Tsao（Mesona procumbens Hemsl.）. Food Res. Int. 33: 487-492

Zaletova N. I., A. N. Shchavlinskii, O. N. Tolkachev. 1986. Preparation of some derivatives of ursolic acid and their antimicrobial activity. Chem. Abs. 106: 18867e